Drought is one of the abiotic stresses limiting the production of soybean(Glycine max).Elucidation of the genetic and molecular basis of the slow-wilting(SW)trait of this crop offers the prospect of its genetic improv...Drought is one of the abiotic stresses limiting the production of soybean(Glycine max).Elucidation of the genetic and molecular basis of the slow-wilting(SW)trait of this crop offers the prospect of its genetic improvement.A panel of 188 accessions and a set of recombinant inbred lines produced from a cross between cultivars Liaodou 14 and Liaodou 21 were used to identify quantitative-trait loci(QTL)associated with SW.Plants were genotyped by Specific-locus amplified fragment sequencing and seedling leaf wilting was assessed under three water-stress treatments.A genome-wide association study identified 26 SW-associated single-nucleotide polymorphisms(SNPs),including three located in a 248-kb linkage-disequilibrium(LD)block on chromosome 2.Linkage mapping revealed a major-effect QTL,qSW2,associated with all three treatments and adjacent to the LD block.Fine mapping in a BC_(2)F_(3) population derived from a backcross between Liaodou 21 and R26 confined qSW2 to a 60-kb interval.Gene expression and sequence variation analysis identified the gene Glyma.02 g218100,encoding an auxin transcription factor,as a candidate gene for qSW2.Our results will contribute significantly to improving drought-resistant soybean cultivars by providing genetic information and resources.展开更多
Changes of RWC, wilting degree and ABA in different ramie varieties under drought stress were studied. The results showed: (1) wilting degree and ABA increased but RWC decreased with the increase of drought sress,and ...Changes of RWC, wilting degree and ABA in different ramie varieties under drought stress were studied. The results showed: (1) wilting degree and ABA increased but RWC decreased with the increase of drought sress,and (2) drought resistant varieties had higher RWC and ABA but lower wilting degree than drought sensitive varieties.展开更多
Wild peanut(Arachis)species are promising sources of disease resistance for improving peanut cultivars.The objective of this study was to assess cross-compatibility among cultivated and wild peanuts in crosses between...Wild peanut(Arachis)species are promising sources of disease resistance for improving peanut cultivars.The objective of this study was to assess cross-compatibility among cultivated and wild peanuts in crosses between eight peanut cultivars and 27 wild species carrying the A,B,E,Ex,F,K,P,and H genomes.Embryo culture and chromosome doubling led to polyploids representing hybrids between cultivated peanut and A.stenosperma,A.macedoi,A.duranensis,A.villosa,and A.diogoi.The first two showed greater resistance to bacterial wilt than their cultivated parents.DNA markers were developed for verifying the hybrids and for identifying translocation or introgression lines with alien chromosome fragments.展开更多
Coffee wilt represents one of the most devastating diseases of Arabica coffee(Coffea arabica L.)plantations in the primary coffee-producing regions.In this study,coffee trees manifesting wilt symptoms accompanied by t...Coffee wilt represents one of the most devastating diseases of Arabica coffee(Coffea arabica L.)plantations in the primary coffee-producing regions.In this study,coffee trees manifesting wilt symptoms accompanied by the defoliation and drying of the whole tree were observed in the Jazan,El Baha,Najran,and Asir regions.The purpose of this investigation was to isolate and identify the Fusarium species recovered from symptomatic coffee trees.The developed fungi were initially characterized based on their morphological features followed by molecular phylogenetic multi-locus analysis of the combined sequences of ITS,TEF1-α,RPB2,and CaM.Twenty-five isolates were recovered from 28 samples.All fungal isolates were categorized morphologically under the genus Fusarium.Phylogenetic analysis positioned all the representative 15 isolates into one cluster grouping together with Neocosmospora falciformis(formerly F.falciforme)confirming their taxonomic position.Pathogenicity tests of the N.falciformis isolates were subsequently conducted on coffee seedlings,and the results revealed that all isolates induced wilt symptoms resembling those recorded in the field,and the incidence was 100%.The fungicide sensitivity test of seven investigated fungicides revealed that Maxim XL^(®) followed by Moncut^(®) exhibited the highest inhibitory effect against N.falciformis KSA 24-14,reaching 93.33%and 91.67%,respectively.To our knowledge,N.falciformis is a new causal pathogen of coffee wilt in Saudi Arabia.Remarkably,these results offer important insights for devising effective approaches to monitor and control such diseases.展开更多
Aim: This study was carried out in order to evaluate the potential of bacteriophages in controlling tomato bacterial wilt disease caused by Ralstonia solanacearum. Study design: A purposive sampling technique was used...Aim: This study was carried out in order to evaluate the potential of bacteriophages in controlling tomato bacterial wilt disease caused by Ralstonia solanacearum. Study design: A purposive sampling technique was used to collect samples from bacterial wilt hot spot tomato growing areas in Kenya. Place and duration of study: The research work was done at Jomo Kenyatta University of Agriculture and Technology, between June 2020 and July 2021. Methodology: Thirty diseased plants and corresponding soil were collected from five counties, Nyeri, Kajiado, Nyandarua, Kiambu and Taita Taveta. Bacteria were isolated and characterized, and then used as hosts to propagate the phages. Tests done were gram stain, oxidation test, potassium hydroxide solubility test, H2S production test catalase test, NaCl test and sugar fermentation test. Molecular analysis and phenotyping were also done in order to identify the bacteria. The bacteriophages were then isolated through a double overlay method using R. solanacearum as the host. They were characterized and assayed in a greenhouse setting to determine their effectiveness in controlling bacterial wilt. Results: Six host bacteria were isolated and all belonged to biovar II. Four phages were observed based on morphology. Upon characterization the phages were stable at 30˚C, PH range between 6 - 7 and tolerance of more than an hour under UV light. In the greenhouse experiment, treatment of plants with bacteriophage prevented wilting after subsequent inoculation with the pathogen. A bacteriophage mix of SN1 and WT1 were used for efficacy tests due to their efficiency in plating and infection. Phage SN1 and WT1 exhibited high lytic activity and relatively high thermotolerance and acid tolerance, thereby showing great potential in the biocontrol of bacterial wilt infection across a variety of conditions. Conclusion: The results obtained in this research show that bacteriophages offer potential for the biocontrol of bacterial wilt.展开更多
Peanut(Arachis hypogaea L.)bacterial wilt(BW)is a devastating soil-borne disease caused by Ralstonia solanacearum(RS)that poses a significant threat to peanut yield and quality.Nucleotide-binding leucine-rich repeat(N...Peanut(Arachis hypogaea L.)bacterial wilt(BW)is a devastating soil-borne disease caused by Ralstonia solanacearum(RS)that poses a significant threat to peanut yield and quality.Nucleotide-binding leucine-rich repeat(NBS-LRR)proteins are a class of plant-specific immune receptors that recognize pathogen-secreted effector molecules and activate immune responses to resist pathogen infections.However,the precise functions of AhCN genes(where CN is a class of nucleotide-binding site,leucine-rich repeat receptor(NLR)genes that lack LRR structural domains)in peanut plants are not fully understood.In this study,a total of 150 AhCN genes were identified and classified into nine subfamilies based on a systematic phylogenetic analysis.The AhCN genes showed highly conserved structural features,and the promoter cis-elements indicated involvement in plant hormone signaling and defense responses.After inoculation with RS,the highly resistant peanut variety‘H108’significantly outperformed the susceptible variety‘H107’based on physiological indicators such as plant height,main stem diameter,and fresh weight,likely due to the inhibition of bacterial proliferation and diffusion in the stem vascular bundle.AhCN34 was found to be significantly upregulated in‘H108’compared to‘H107’during plant infection and in response to treatments with each of three plant hormones.Importantly,AhCN34 overexpression in peanut leaves enhanced their resistance to BW.These findings demonstrate the great potential of AhCN34 for applications in peanut resistance breeding.Our identification and characterization of the AhCN genes provide insights into the mechanisms underlying BW resistance in peanut and can inform future research into genetic methods of improving BW resistance in peanut.展开更多
Multiparent advanced generation inter-cross(MAGIC)populations improve the accuracy of quantitative trait loci mapping compared to biparental populations by increasing diversity and reducing linkage disequilibrium betw...Multiparent advanced generation inter-cross(MAGIC)populations improve the accuracy of quantitative trait loci mapping compared to biparental populations by increasing diversity and reducing linkage disequilibrium between variants.Here we describe the development of two MAGIC populations derived from a cross of 11 founder lines from the INTA(National Institute for Agricultural Technology in Argentina)sunflower breeding program.The founder lines are fertility maintainer inbred lines that exhibit genetic diversity in several traits,including flowering cycle,plant architecture,disease resistance,oil content and oil quality.The crossing scheme applied follows the design of two-way,four-way and eight-way crosses with a modification to increase the number of recombinations and shorten the linkage disequilibrium.Phenotyping a subset of F_(2)families and F_5 lines for different traits shows an increase in the diversity of the analyzed traits compared to the parental lines.In addition,we identified lines with transgressive segregation for Sunflower Verticillium Wilt resistance.These results confirm that the MAGIC populations will serve as unique genetic and genomic resources to better characterize the genetics of complex traits and identify potentially superior alleles for sunflower breeding.It will also enrich the gene pool of fertility maintainers(A/B line)of the sunflower germplasm and facilitate the introduction of new breeding methods to select female parents for use in AxR combinations to obtain superior sunflower hybrids.展开更多
Bacterial wilt(BW)caused by Ralstonia solanacearum is a wide-spread and serious disease in peanut.To date,this soilborne disease could only be effectively controlled by planting resistant peanut cultivars.However,the ...Bacterial wilt(BW)caused by Ralstonia solanacearum is a wide-spread and serious disease in peanut.To date,this soilborne disease could only be effectively controlled by planting resistant peanut cultivars.However,the relatively lower yield potential of the available BW-resistant peanut cultivars is a key reason restricting productivity in most epidemic regions naturally infested with the pathogen.Even small pods or seeds and low number per plant has been regarded as the key factor for the low yield potential both in BW-resistant peanut germplasm lines and available released cultivars,whether the resistance is closely linked with key yield components remains unclear.In this study,the relationship between pod weight and BW resistance was analyzed by using a recombinant inbred lines(RIL)population derived from a crossing combination between a high yielding cultivar Xuhua 13 and a BW-resistant cultivar Zhonghua 6.From the experiments,it was found that the BW resistance was not significantly correlated with pod number per plant(PNP),hundred pod weight(HPW)and pod weight per plant(PWP)in the RIL population.Based on linkage analysis,the quantitative trait locus(QTL)s related to PNP were identified on A06,A07,A08 and B03.The QTLs for HPW were detected on A05 and A07,and the QTLs for PWP were on A06,A07 and B03.However,the QTL for BW resistance identified on B02.These results indicated that the BW resistance and the pod number per plant as well as pod weight were inherited independently.Two recombined lines(QT0944 and QT1028)with high level BW resistance and large pods(hundred pod weight over 185g)were identified from the RILs,and they possessed the favored alleles of identified QTLs from both parents,which could be used in peanut breeding for high yield and high level disease resistance.展开更多
The effectiveness of fluopyram suspension concentrate against pine wilt disease(PWD)is limited by spraying efficiency and water dependence.A traditional dust formulation with strong dispersibility can overcome these s...The effectiveness of fluopyram suspension concentrate against pine wilt disease(PWD)is limited by spraying efficiency and water dependence.A traditional dust formulation with strong dispersibility can overcome these shortcomings.However,its efficacy against PWD remains uncertain.This study evaluated the translocation of fluopyram dust within tree tissues,soil and water degradation,and its effective control against PWD.Nursery tests showed effective prevention;field tests showed dust absorption and translocation into pine tissues.Thirty days following application,residual concentrations in soil were low at 0.09 mg kg^(−1);no detectable residues were found in water samples.Three years after applying fluopyram,its effectiveness increased to approximately 87%.Based on this study,fluopyram had a half-life of 346 d with persistence lasting up to three years.This provides valuable insight for managing PWD through dust applications.展开更多
Background Cotton is an important cash crop in China and a key component of the global textile market.Verticil-lium wilt is a major factor affecting cotton yield.Single nucleotide polymorphism(SNP)markers and phenotyp...Background Cotton is an important cash crop in China and a key component of the global textile market.Verticil-lium wilt is a major factor affecting cotton yield.Single nucleotide polymorphism(SNP)markers and phenotypic data can be used to identify genetic markers and loci associated with cotton resistance to Verticillium wilt.We used eight upland cotton parent materials in this study to construct a multiparent advanced generation inter-cross(MAGIC)population comprising 320 lines.The Verticillium wilt resistance of the MAGIC population was identified in the green-house in 2019,and the average relative disease index(ARDI)was calculated.A genome-wide association study(GWAS)was performed to discover SNP markers/genes associated with Verticillium wilt resistance.Results ARDI of the MAGIC population showed wide variation,ranging from 16.7 to 79.4 across three replicates.This variation reflected a diverse range of resistance to Verticillium wilt within the population.Analysis of distribution pat-terns across the environments revealed consistent trends,with coefficients of variation between 12.25%and 21.96%.Families with higher ARDI values,indicating stronger resistance,were more common,likely due to genetic diver-sity and environmental factors.Population structure analysis divided the MAGIC population into three subgroups,with Group I showing higher genetic variation and Groups II and III displaying more uniform resistance performance.Principal component analysis(PCA)confirmed these divisions,highlighting the genetic diversity underlying Verticil-lium wilt resistance.Through GWAS,we identified 19 SNPs significantly associated with Verticillium wilt resistance,distributed across three chromosomes.The screening of candidate genes was performed on the transcriptome derived from resistant and susceptible cultivars,combined with gene annotation and tissue expression patterns,and two key candidate genes,Ghir_A01G006660 and Ghir_A02G008980,were found to be potentially associated with Verticillium wilt resistance.This suggests that these two candidate genes may play an important role in responding to Verticillium wilt.Conclusion This study aims to dissect the genetic basis of Verticillium wilt resistance in cotton by using a MAGIC population and GWAS.The study seeks to provide valuable genetic resources for marker-assisted breeding and enhance the understanding of resistance mechanisms to improve cotton resilience against Verticillium wilt.展开更多
Tomato bacterial wilt is an important disease caused by Ralstonia solanacearum,which is harmful to the development of tomato industry and seriously affects the yield and quality of tomato.In this study,the strain XF-8...Tomato bacterial wilt is an important disease caused by Ralstonia solanacearum,which is harmful to the development of tomato industry and seriously affects the yield and quality of tomato.In this study,the strain XF-8 with antagonism against R.solanacearum was isolated from soil and identified as Bacillus velezensis,and its control effect on tomato bacterial wilt was better than that of kasugamycin.The disease index of tomato plants treated with XF-8 was significantly reduced(P<0.05)in the pot experiment,and the control effect was 74.25%,which was higher than 45.11%of kasugamycin treatment group.The antibacterial activity of lipopeptide extract from the fermentation supernatant of strain XF-8 was further verified,and then the inhibitory rate of the substance at the concentration of 1 mg/mL was as high as 96.20%.The microscopic investigations indicated that the surface of R.solanacearum was wrinkled and deformed after treating with lipopeptide extract,and most of the bacteria were dead and rupture of the cell membrane,which leading to the cell death.Meanwhile,the active oxygen was produced and the lipopeptide extract could also significantly induced DNA injuries of R.solanacearum.All these results confirmed that the isolated strain B.velezensis exhibited excellent effect of preventing and controlling the tomato bacterial wilt.Moreover,XF-8 strain has high inhibitory effect on 10 types of pathogenic fungi,which shows broad-spectrum bacteriostasis.This study provides a theoretical basis and strain resources for the development and application of biopesticides,and broadened the potential biocontrol value of B.velezensis.展开更多
In order to elucidate the relationship between the pathogen carriage rate in seeds of muskmelon(Cucumis melo L.)and the incidence rate of Fusarium wilt of muskmelon(FWM),as well as to identify potential biological con...In order to elucidate the relationship between the pathogen carriage rate in seeds of muskmelon(Cucumis melo L.)and the incidence rate of Fusarium wilt of muskmelon(FWM),as well as to identify potential biological control agents against FWM,this study conducted both pot and field experiments to evaluate the efficacy of Gongzhulingmycin on FWM and its impact on muskmelon yield.The results indicated that the pathogen carriage rates of different species in muskmelon seeds varied significantly,showing a positive correlation with disease incidence during the seedling stage.The results from pot tests indicated that in comparison to the control,disease indices were significantly reduced following treatment with prochloraz and 100 times Gongzhulingmycin at both 7 days and 14 days post-emergence of FWM symptoms.Concurrently,root growth was enhanced.Field experiment outcomes demonstrated that relative to the control,there was a decrease in FWM incidence during the fruit-setting stage,along with an increase in theoretical output per square kilometer for muskmelon.Although the efficacy of Gongzhulingmycin against FWM was lower than that observed for prochloraz,it exhibited significant effects on biomass enhancement and disease resistance.Therefore,it showed promise as a potential biological control agent for managing FWM.展开更多
Verticillium wilt,caused by the infamous pathogen Verticillium dahliae,presents a primary constraint on cotton cul-tivation worldwide.The complexity of disease resistance in cotton and the largely unexplored interacti...Verticillium wilt,caused by the infamous pathogen Verticillium dahliae,presents a primary constraint on cotton cul-tivation worldwide.The complexity of disease resistance in cotton and the largely unexplored interaction dynamics between the cotton plant host and V.dahliae pathogen pose a crucial predicament for effectively managing cotton Verticillium wilt.Nevertheless,the most cost-effective approach to controlling this disease involves breeding and cul-tivating resistant cotton varieties,demanding a meticulous analysis of the mechanisms underlying cotton’s resistance to Verticillium wilt and the identification of pivotal genes.These aspects constitute focal points in disease-resistance breeding programs.In this review,we comprehensively discuss genetic inheritance associated with Verticillium wilt resistance in cotton,the advancements in molecular markers for disease resistance,the functional investiga-tion of resistance genes in cotton,the analysis of pathogenicity genes in V.dahliae,as well as the intricate interplay between cotton and this fungus.Moreover,we delve into the future prospects of cutting-edge research on cotton Verticillium wilt,aiming to proffer valuable insights for the effective management of this devastating fungus.展开更多
Vascular wilt caused by Fusarium oxysporum f.sp.batatas(Fob)is a devastating disease threatening global sweet potato production.To elucidate Fob’s pathogenicitymechanisms and informeffective control strategies,we gen...Vascular wilt caused by Fusarium oxysporum f.sp.batatas(Fob)is a devastating disease threatening global sweet potato production.To elucidate Fob’s pathogenicitymechanisms and informeffective control strategies,we generated a green fluorescent protein(GFP)-tagged Fob strain to track infection dynamics in sweet potato susceptible cultivar Xinzhonghua and resistant cultivar Xiangshu75-55,respectively.Through cytological observation,we found in the susceptible Xinzhonghua,Fob predominantly colonized stem villi,injured root growth points,and directly invaded vascular bundles through stemwounds.Spore germination peaked at 2-3 h post-inoculation(hpi),followed by cyclical mycelial expansion and sporulation within vascular tissues with sustaining infection.In contrast,the resistant Xiangshu75-55 exhibited strong suppression of Fob:spores rarely germinated in vascular bundles or on trichomes by 3 hpi,and mature hyphae were absent in stems at 24 hpi.Quantitative reverse transcription PCR(qRT-PCR)confirmed significantly higher Fob biomass in Xinzhonghua than in Xiangshu75-55 by 16 hpi.Additionally,transcriptional profiling revealed distinct pathogen-host interactions during the compatible and incompatible reactions.In Xinzhonghua,Fob virulence genes FobPGX1,FobICL1,FobCTF2,FobFUB5 and FobFUB6 were upregulated within 16 hpi.Conversely,host defense genes IbMAPKK9,IbWRKY61,IbWRKY75,IbSWEET10,IbBBX24 and IbPIF4 were activated in Xiangshu75-55 during the same period.This study provides spatiotemporal cytological and molecular insights into Fob pathogenicity and host resistance,offering a foundation for early disease detection and improved Fusarium wilt management in sweet potato.展开更多
Bacterial wilt （BW） caused by Ralstonia solanacearum is an important constraint to peanut （Arachis hypogaea L.） production in several Asian and African countries, and planting BW-resistant cultivars is the most fe...Bacterial wilt （BW） caused by Ralstonia solanacearum is an important constraint to peanut （Arachis hypogaea L.） production in several Asian and African countries, and planting BW-resistant cultivars is the most feasible method for controlling the disease. Although several BW-resistant peanut germplasm accessions have been identified, the genetic diversity among these has not been properly investigated, which has impeded efficient utilization. In this study, the genetic relationships of 31 peanut genotypes with various levels of resistance to BW were assessed based on SSR and AFLP analyses. Twenty-nine of 78 SSR primers and 32 of 126 AFLP primer combinations employed in this study were polymorphic amongst the peanut genotypes tested. The SSR primers amplified 91 polymorphic loci in total with an average of 3.14 alleles per primer, and the AFLP primers amplified 72 polymorphic loci in total with an average of 2.25 alleles per primer. Four SSR primers （14H06, 7G02, 3A8, 16C6） and one AFLP primer （P1M62） were found to be most efficient in detecting diversity. The genetic distance between pairs of genotypes ranged from 0.12 to 0.94 with an average of 0.53 in the SSR data and from 0.06 to 0.57 with an average of 0.25 in the AFLP data. The SSR-based estimates of the genetic distance were generally larger than that based on the AFLP data. The genotypes belonging to subsp, fastigiata possessed wider diversity than that of subsp, hypogaea. The clustering of genotypes based on the SSR and AFLP data were similar but the SSR clustering was more consistent with morphological classification ofA. hypogaea. Optimum diverse genotypes of both subsp, hypogaea and subsp.fastigiata can be recommended based on this analysis for developing mapping populations and breeding for high yielding and resistant cultivars.展开更多
[Objective] The aim of this study was to breed new strains which have higher inhibitory effects on the pathogens of watermelon fusarium wilt.[Method] The endophytic Bacillus subtilis B47 strain was obtained from tomat...[Objective] The aim of this study was to breed new strains which have higher inhibitory effects on the pathogens of watermelon fusarium wilt.[Method] The endophytic Bacillus subtilis B47 strain was obtained from tomato stems by UV mutagenesis for two consecutive times,then genetic stability as well as physiological and biochemical properties of mutant strains were studied.[Result] The antibacterial activity of all the three mutant strains F303,F304 and F305 was higher than that of B74 strain.After subculture of 10 successive generations,the antibacterial activity of all the three mutant strains for the pathogens of watermelon fusarium wilt decreased,but the antibacterial activity of F305 strain decreased the least,indicating its best genetic stability among the tested strains.The antibacterial circle diameter of F305 strain was 5 mm larger than that of wild strain B47 under the same condition.The mutant strain F305 was in logarithmic growth phase within 36 h and in stationary phase within 36-96 h,while its optimum growth temperature was 35 ℃.F305 strain could grow in sodium salt with the concentration of 1%-10%,but it grew best at the concentration of 1%.Physiological and biochemical responses of F305 strain were in accordance with those of wild strain B47.[Conclusion] This study lays the foundation for the factorial production of antagonistic substance by B47 strain and new methods of preventing from the pathogens watermelon fusarium wilt.展开更多
[Objective] The aim of this study was to screen bacteria strains with stable antagonistic effect against watermelon fusarium wilt from soil and investigate the biological control of watermelon fusarium wilt by applyin...[Objective] The aim of this study was to screen bacteria strains with stable antagonistic effect against watermelon fusarium wilt from soil and investigate the biological control of watermelon fusarium wilt by applying the antagonistic bacteria strains into soil.[Method] Actinomycete strains,fluorescent bacteria strains and bacillus strains were isolated from soil samples by the dilution-plate method,then its resistance was screened respectively by the improved confront culture method after colonies were purified.Finally,bacteria strains with better antagonistic effect were identified.[Result] 29 bacteria strains with stable antagonistic effect against watermelon fusarium wilt were screened from 39 soil samples,which contained 15 fluorescent bacteria strains,5 bacillus strains and 9 actinomycete strains.Furthermore,three antagonistic bacteria strains of FM2,FM3 and FM4 with the strongest antagonism were identified primarily.[Conclusion] According to cultural characteristics,morphological observation,biochemical and physiological tests,FM2 belongs to bacillus subtilis,while FM3 and FM4 belong to micrococcus.展开更多
[Objective] Aimed to lay a scientific foundation for eggplant resistant breeding, the resistant field identification of eggplant resources to Verticillium wilt was carried out. [Method] Test materials were 35 eggplant...[Objective] Aimed to lay a scientific foundation for eggplant resistant breeding, the resistant field identification of eggplant resources to Verticillium wilt was carried out. [Method] Test materials were 35 eggplant resources, which were collected from various places in Sichuan province since 2001. These eggplant resources were made resistance evaluation to Verticillium wilt by watering injured roots with Verticillium dahliae spore suspension. [Result] The eggplant resources had good resistance to Verticillium wilt. Among 35 evaluated resources, 10 resources like 242-9OP, 41-1,35-R5, etc. were resistant, while 23 resources like 68 ×47, Zizaoqie, 32-1, etc. were tolerant. Some resistant resources like 41-1,35-R5, 47-R1, etc. had been transferred into new resistant varieties like EF9910, etc. by breeding institutes. [Conclusion] The resistant level and future resistance breeding direction of eggplant resources in Sichuan were clarified by the resistance evaluation of eggplant resources so as to provide a technical guidance for eggplant resistance breeding.展开更多
[Objectives] This paper aims to explore the possibility to intercrop garlic with pomegranate tree to control pomegranate wilt.[Methods] Root exudates of garlic is cultivated in 1/5 concentration of MS solution and dis...[Objectives] This paper aims to explore the possibility to intercrop garlic with pomegranate tree to control pomegranate wilt.[Methods] Root exudates of garlic is cultivated in 1/5 concentration of MS solution and distilled water is examined in lab to test their effect to growth of mycelia of pomegranate wilt pathogen（Ceratocystis fimbriata）and multiplication of Bacillus subtilis.[Results] The result shows that garlic root exudates whatever cultivated in MS solution or distilled water could not inhibit or promote mycelia growth of C.fimbriata.However,garlic root exudates cultivated in both methods effectively promote multiplication of B.subtilis.[Conclusions] It is suggested that intercropping garlic with pomegranate tree by combining application B.subtilis could be a promising way to prevent pomegranate wilt spread in practice.展开更多
基金The study was supported by the National Natural Science Foundation of China(32101795,32301782)National Key Research and Development Program of China(2016YFD0100201-01)+2 种基金Liaoning Provincial Major Special Project of Agricultural Science and Technology(2022JH1/10200002,2021JH1/10400038)Key Research and Development Plan of Liaoning Science and Technology Department(2021JH2/1020027)Shenyang Seed Industry Innovation Project(22-318-2-12).
文摘Drought is one of the abiotic stresses limiting the production of soybean(Glycine max).Elucidation of the genetic and molecular basis of the slow-wilting(SW)trait of this crop offers the prospect of its genetic improvement.A panel of 188 accessions and a set of recombinant inbred lines produced from a cross between cultivars Liaodou 14 and Liaodou 21 were used to identify quantitative-trait loci(QTL)associated with SW.Plants were genotyped by Specific-locus amplified fragment sequencing and seedling leaf wilting was assessed under three water-stress treatments.A genome-wide association study identified 26 SW-associated single-nucleotide polymorphisms(SNPs),including three located in a 248-kb linkage-disequilibrium(LD)block on chromosome 2.Linkage mapping revealed a major-effect QTL,qSW2,associated with all three treatments and adjacent to the LD block.Fine mapping in a BC_(2)F_(3) population derived from a backcross between Liaodou 21 and R26 confined qSW2 to a 60-kb interval.Gene expression and sequence variation analysis identified the gene Glyma.02 g218100,encoding an auxin transcription factor,as a candidate gene for qSW2.Our results will contribute significantly to improving drought-resistant soybean cultivars by providing genetic information and resources.
文摘Changes of RWC, wilting degree and ABA in different ramie varieties under drought stress were studied. The results showed: (1) wilting degree and ABA increased but RWC decreased with the increase of drought sress,and (2) drought resistant varieties had higher RWC and ABA but lower wilting degree than drought sensitive varieties.
基金supported by National Natural Science Foundation of China(32272153)Henan Province Science and Technology R&D Joint Fund(232301420025)+4 种基金National Key Research and Development Program of China(2023YFD1200200)the Key Research Project of the Shennong Laboratory(SN01-2022-03)Independent Innovation Foundation of Henan Academy of Agricultural Sciences(2024ZC024)China Agriculture Research System(CARS-13)Henan Provincial Agriculture Research System(S2012-5).
文摘Wild peanut(Arachis)species are promising sources of disease resistance for improving peanut cultivars.The objective of this study was to assess cross-compatibility among cultivated and wild peanuts in crosses between eight peanut cultivars and 27 wild species carrying the A,B,E,Ex,F,K,P,and H genomes.Embryo culture and chromosome doubling led to polyploids representing hybrids between cultivated peanut and A.stenosperma,A.macedoi,A.duranensis,A.villosa,and A.diogoi.The first two showed greater resistance to bacterial wilt than their cultivated parents.DNA markers were developed for verifying the hybrids and for identifying translocation or introgression lines with alien chromosome fragments.
基金funded by the Deanship of Scientific Research,Vice Presidency for Graduate Studies and Scientific Research,King Faisal University,Saudi Arabia,for supporting this work for work through grant number KFU242134.
文摘Coffee wilt represents one of the most devastating diseases of Arabica coffee(Coffea arabica L.)plantations in the primary coffee-producing regions.In this study,coffee trees manifesting wilt symptoms accompanied by the defoliation and drying of the whole tree were observed in the Jazan,El Baha,Najran,and Asir regions.The purpose of this investigation was to isolate and identify the Fusarium species recovered from symptomatic coffee trees.The developed fungi were initially characterized based on their morphological features followed by molecular phylogenetic multi-locus analysis of the combined sequences of ITS,TEF1-α,RPB2,and CaM.Twenty-five isolates were recovered from 28 samples.All fungal isolates were categorized morphologically under the genus Fusarium.Phylogenetic analysis positioned all the representative 15 isolates into one cluster grouping together with Neocosmospora falciformis(formerly F.falciforme)confirming their taxonomic position.Pathogenicity tests of the N.falciformis isolates were subsequently conducted on coffee seedlings,and the results revealed that all isolates induced wilt symptoms resembling those recorded in the field,and the incidence was 100%.The fungicide sensitivity test of seven investigated fungicides revealed that Maxim XL^(®) followed by Moncut^(®) exhibited the highest inhibitory effect against N.falciformis KSA 24-14,reaching 93.33%and 91.67%,respectively.To our knowledge,N.falciformis is a new causal pathogen of coffee wilt in Saudi Arabia.Remarkably,these results offer important insights for devising effective approaches to monitor and control such diseases.
文摘Aim: This study was carried out in order to evaluate the potential of bacteriophages in controlling tomato bacterial wilt disease caused by Ralstonia solanacearum. Study design: A purposive sampling technique was used to collect samples from bacterial wilt hot spot tomato growing areas in Kenya. Place and duration of study: The research work was done at Jomo Kenyatta University of Agriculture and Technology, between June 2020 and July 2021. Methodology: Thirty diseased plants and corresponding soil were collected from five counties, Nyeri, Kajiado, Nyandarua, Kiambu and Taita Taveta. Bacteria were isolated and characterized, and then used as hosts to propagate the phages. Tests done were gram stain, oxidation test, potassium hydroxide solubility test, H2S production test catalase test, NaCl test and sugar fermentation test. Molecular analysis and phenotyping were also done in order to identify the bacteria. The bacteriophages were then isolated through a double overlay method using R. solanacearum as the host. They were characterized and assayed in a greenhouse setting to determine their effectiveness in controlling bacterial wilt. Results: Six host bacteria were isolated and all belonged to biovar II. Four phages were observed based on morphology. Upon characterization the phages were stable at 30˚C, PH range between 6 - 7 and tolerance of more than an hour under UV light. In the greenhouse experiment, treatment of plants with bacteriophage prevented wilting after subsequent inoculation with the pathogen. A bacteriophage mix of SN1 and WT1 were used for efficacy tests due to their efficiency in plating and infection. Phage SN1 and WT1 exhibited high lytic activity and relatively high thermotolerance and acid tolerance, thereby showing great potential in the biocontrol of bacterial wilt infection across a variety of conditions. Conclusion: The results obtained in this research show that bacteriophages offer potential for the biocontrol of bacterial wilt.
基金supported by the grants from the National Natural Science Foundation of China(NSFC)-Henan United Fund(U22A20475 and U1704232)the Key Scientific and Technological Project of Henan Province,China(221111110500,161100111000,and HARS-22-05-G1)+2 种基金the Innovation Scientists and Technicians Troop Construction Projects of Henan Province,China(2018JR0001)the Henan Agricultural University High Level Talent Special Support Fund,China(30501418)the Key Scientific Research Project in Colleges and Universities of Henan Province,China(21A210018).
文摘Peanut(Arachis hypogaea L.)bacterial wilt(BW)is a devastating soil-borne disease caused by Ralstonia solanacearum(RS)that poses a significant threat to peanut yield and quality.Nucleotide-binding leucine-rich repeat(NBS-LRR)proteins are a class of plant-specific immune receptors that recognize pathogen-secreted effector molecules and activate immune responses to resist pathogen infections.However,the precise functions of AhCN genes(where CN is a class of nucleotide-binding site,leucine-rich repeat receptor(NLR)genes that lack LRR structural domains)in peanut plants are not fully understood.In this study,a total of 150 AhCN genes were identified and classified into nine subfamilies based on a systematic phylogenetic analysis.The AhCN genes showed highly conserved structural features,and the promoter cis-elements indicated involvement in plant hormone signaling and defense responses.After inoculation with RS,the highly resistant peanut variety‘H108’significantly outperformed the susceptible variety‘H107’based on physiological indicators such as plant height,main stem diameter,and fresh weight,likely due to the inhibition of bacterial proliferation and diffusion in the stem vascular bundle.AhCN34 was found to be significantly upregulated in‘H108’compared to‘H107’during plant infection and in response to treatments with each of three plant hormones.Importantly,AhCN34 overexpression in peanut leaves enhanced their resistance to BW.These findings demonstrate the great potential of AhCN34 for applications in peanut resistance breeding.Our identification and characterization of the AhCN genes provide insights into the mechanisms underlying BW resistance in peanut and can inform future research into genetic methods of improving BW resistance in peanut.
基金supported by a doctoral grant from the National Scientific and Technical Research Council(Consejo Nacional de Investigaciones Científicas y T ecnicas,CONICET)the National Institute of Agricultural Technology(INTA)+1 种基金by projects funded by INTA(CP_2019–2023 I-114,I-127,I-090/2023–2025 I-111,I-87)Agencia Nacional de Promocio n Científica y T ecnica(PICT 2017N?2523)。
文摘Multiparent advanced generation inter-cross(MAGIC)populations improve the accuracy of quantitative trait loci mapping compared to biparental populations by increasing diversity and reducing linkage disequilibrium between variants.Here we describe the development of two MAGIC populations derived from a cross of 11 founder lines from the INTA(National Institute for Agricultural Technology in Argentina)sunflower breeding program.The founder lines are fertility maintainer inbred lines that exhibit genetic diversity in several traits,including flowering cycle,plant architecture,disease resistance,oil content and oil quality.The crossing scheme applied follows the design of two-way,four-way and eight-way crosses with a modification to increase the number of recombinations and shorten the linkage disequilibrium.Phenotyping a subset of F_(2)families and F_5 lines for different traits shows an increase in the diversity of the analyzed traits compared to the parental lines.In addition,we identified lines with transgressive segregation for Sunflower Verticillium Wilt resistance.These results confirm that the MAGIC populations will serve as unique genetic and genomic resources to better characterize the genetics of complex traits and identify potentially superior alleles for sunflower breeding.It will also enrich the gene pool of fertility maintainers(A/B line)of the sunflower germplasm and facilitate the introduction of new breeding methods to select female parents for use in AxR combinations to obtain superior sunflower hybrids.
基金supported by the National Natural Science Foundation of China(32161143006,32171997)the Earmarked Fund for CARS-13,and the Agricultural Science and Technology Innovation Program of Chinese Academy of Agricultural Sciences(No.CAAS-ASTIP-2021-OCRI).
文摘Bacterial wilt(BW)caused by Ralstonia solanacearum is a wide-spread and serious disease in peanut.To date,this soilborne disease could only be effectively controlled by planting resistant peanut cultivars.However,the relatively lower yield potential of the available BW-resistant peanut cultivars is a key reason restricting productivity in most epidemic regions naturally infested with the pathogen.Even small pods or seeds and low number per plant has been regarded as the key factor for the low yield potential both in BW-resistant peanut germplasm lines and available released cultivars,whether the resistance is closely linked with key yield components remains unclear.In this study,the relationship between pod weight and BW resistance was analyzed by using a recombinant inbred lines(RIL)population derived from a crossing combination between a high yielding cultivar Xuhua 13 and a BW-resistant cultivar Zhonghua 6.From the experiments,it was found that the BW resistance was not significantly correlated with pod number per plant(PNP),hundred pod weight(HPW)and pod weight per plant(PWP)in the RIL population.Based on linkage analysis,the quantitative trait locus(QTL)s related to PNP were identified on A06,A07,A08 and B03.The QTLs for HPW were detected on A05 and A07,and the QTLs for PWP were on A06,A07 and B03.However,the QTL for BW resistance identified on B02.These results indicated that the BW resistance and the pod number per plant as well as pod weight were inherited independently.Two recombined lines(QT0944 and QT1028)with high level BW resistance and large pods(hundred pod weight over 185g)were identified from the RILs,and they possessed the favored alleles of identified QTLs from both parents,which could be used in peanut breeding for high yield and high level disease resistance.
基金supported by grants from the National Key R&D Program of China(grant number 2021YFD1400900)the National Natural Science Foundation of China(grant numbers U1905201,32171805)+6 种基金the Forestry Key Program of Science and Technology in Fujian Province(grant number 2021FKJ03)the Natural Science Foundation of Fujian Province,China(grant number 2021J01056)the Forestry Programs of Science and Technology in Fujian Province[grant number Mincaizhi(2020)601]the Science and Technology Program of Fujian Province(grant number 2018N5002)the Forestry Science Research Project of Fujian Forestry Department[grant number Minlinke(2017)03]the National Major Emergency Science and Technology Program of China(grant number ZD202001)the Forestry Peak Discipline Construction Project of Fujian Agriculture and Forestry University(grant numbers 72202200205,71201800720).
文摘The effectiveness of fluopyram suspension concentrate against pine wilt disease(PWD)is limited by spraying efficiency and water dependence.A traditional dust formulation with strong dispersibility can overcome these shortcomings.However,its efficacy against PWD remains uncertain.This study evaluated the translocation of fluopyram dust within tree tissues,soil and water degradation,and its effective control against PWD.Nursery tests showed effective prevention;field tests showed dust absorption and translocation into pine tissues.Thirty days following application,residual concentrations in soil were low at 0.09 mg kg^(−1);no detectable residues were found in water samples.Three years after applying fluopyram,its effectiveness increased to approximately 87%.Based on this study,fluopyram had a half-life of 346 d with persistence lasting up to three years.This provides valuable insight for managing PWD through dust applications.
基金supported by funding from the fund for National Key Research and Development Program of China(2023YFD2301203-05)National Natural Science Foundation of China(32260510)+3 种基金Special Financial Project for Seed Industry Development in the Autonomous Region(BNZJ2024-10,BNZJ2024-30)Key Project for Science and Technology Development of Shihezi city,Xinjiang Production and Construction Crops(2022NY01)Shihezi University high-level talent research project(RCZK202337)Science and Technol-ogy Planning of Shuanghe city,Xinjiang Production and Construction Crops(2021NY02).
文摘Background Cotton is an important cash crop in China and a key component of the global textile market.Verticil-lium wilt is a major factor affecting cotton yield.Single nucleotide polymorphism(SNP)markers and phenotypic data can be used to identify genetic markers and loci associated with cotton resistance to Verticillium wilt.We used eight upland cotton parent materials in this study to construct a multiparent advanced generation inter-cross(MAGIC)population comprising 320 lines.The Verticillium wilt resistance of the MAGIC population was identified in the green-house in 2019,and the average relative disease index(ARDI)was calculated.A genome-wide association study(GWAS)was performed to discover SNP markers/genes associated with Verticillium wilt resistance.Results ARDI of the MAGIC population showed wide variation,ranging from 16.7 to 79.4 across three replicates.This variation reflected a diverse range of resistance to Verticillium wilt within the population.Analysis of distribution pat-terns across the environments revealed consistent trends,with coefficients of variation between 12.25%and 21.96%.Families with higher ARDI values,indicating stronger resistance,were more common,likely due to genetic diver-sity and environmental factors.Population structure analysis divided the MAGIC population into three subgroups,with Group I showing higher genetic variation and Groups II and III displaying more uniform resistance performance.Principal component analysis(PCA)confirmed these divisions,highlighting the genetic diversity underlying Verticil-lium wilt resistance.Through GWAS,we identified 19 SNPs significantly associated with Verticillium wilt resistance,distributed across three chromosomes.The screening of candidate genes was performed on the transcriptome derived from resistant and susceptible cultivars,combined with gene annotation and tissue expression patterns,and two key candidate genes,Ghir_A01G006660 and Ghir_A02G008980,were found to be potentially associated with Verticillium wilt resistance.This suggests that these two candidate genes may play an important role in responding to Verticillium wilt.Conclusion This study aims to dissect the genetic basis of Verticillium wilt resistance in cotton by using a MAGIC population and GWAS.The study seeks to provide valuable genetic resources for marker-assisted breeding and enhance the understanding of resistance mechanisms to improve cotton resilience against Verticillium wilt.
基金supported by the National Key R&D Program of China(grant no.2022YFD1400700)the Fujian Agriculture and Forestry University Construction Project for the Technological Innovation and Service System of the Tea Industry Chain(K1520005A03)+1 种基金The Open Funds of the State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops,Fujian Agriculture and Forestry University(SKL2023005)The Special Fund for Scientific and Technological Innovation of Fujian Agriculture and Forestry University(KFB23012).
文摘Tomato bacterial wilt is an important disease caused by Ralstonia solanacearum,which is harmful to the development of tomato industry and seriously affects the yield and quality of tomato.In this study,the strain XF-8 with antagonism against R.solanacearum was isolated from soil and identified as Bacillus velezensis,and its control effect on tomato bacterial wilt was better than that of kasugamycin.The disease index of tomato plants treated with XF-8 was significantly reduced(P<0.05)in the pot experiment,and the control effect was 74.25%,which was higher than 45.11%of kasugamycin treatment group.The antibacterial activity of lipopeptide extract from the fermentation supernatant of strain XF-8 was further verified,and then the inhibitory rate of the substance at the concentration of 1 mg/mL was as high as 96.20%.The microscopic investigations indicated that the surface of R.solanacearum was wrinkled and deformed after treating with lipopeptide extract,and most of the bacteria were dead and rupture of the cell membrane,which leading to the cell death.Meanwhile,the active oxygen was produced and the lipopeptide extract could also significantly induced DNA injuries of R.solanacearum.All these results confirmed that the isolated strain B.velezensis exhibited excellent effect of preventing and controlling the tomato bacterial wilt.Moreover,XF-8 strain has high inhibitory effect on 10 types of pathogenic fungi,which shows broad-spectrum bacteriostasis.This study provides a theoretical basis and strain resources for the development and application of biopesticides,and broadened the potential biocontrol value of B.velezensis.
基金Supported by the Key Research and Development Project of the Department of Science and Technology of Jilin Province(20230203175SF)。
文摘In order to elucidate the relationship between the pathogen carriage rate in seeds of muskmelon(Cucumis melo L.)and the incidence rate of Fusarium wilt of muskmelon(FWM),as well as to identify potential biological control agents against FWM,this study conducted both pot and field experiments to evaluate the efficacy of Gongzhulingmycin on FWM and its impact on muskmelon yield.The results indicated that the pathogen carriage rates of different species in muskmelon seeds varied significantly,showing a positive correlation with disease incidence during the seedling stage.The results from pot tests indicated that in comparison to the control,disease indices were significantly reduced following treatment with prochloraz and 100 times Gongzhulingmycin at both 7 days and 14 days post-emergence of FWM symptoms.Concurrently,root growth was enhanced.Field experiment outcomes demonstrated that relative to the control,there was a decrease in FWM incidence during the fruit-setting stage,along with an increase in theoretical output per square kilometer for muskmelon.Although the efficacy of Gongzhulingmycin against FWM was lower than that observed for prochloraz,it exhibited significant effects on biomass enhancement and disease resistance.Therefore,it showed promise as a potential biological control agent for managing FWM.
基金supported by National Natural Science Foundation of China(32201752)Xinjiang Tianchi Talents Program (TCYC2023TP02)Key Project of the Natural Science Foundation of Xinjiang Production and Construction Corps (2024DA001)
文摘Verticillium wilt,caused by the infamous pathogen Verticillium dahliae,presents a primary constraint on cotton cul-tivation worldwide.The complexity of disease resistance in cotton and the largely unexplored interaction dynamics between the cotton plant host and V.dahliae pathogen pose a crucial predicament for effectively managing cotton Verticillium wilt.Nevertheless,the most cost-effective approach to controlling this disease involves breeding and cul-tivating resistant cotton varieties,demanding a meticulous analysis of the mechanisms underlying cotton’s resistance to Verticillium wilt and the identification of pivotal genes.These aspects constitute focal points in disease-resistance breeding programs.In this review,we comprehensively discuss genetic inheritance associated with Verticillium wilt resistance in cotton,the advancements in molecular markers for disease resistance,the functional investiga-tion of resistance genes in cotton,the analysis of pathogenicity genes in V.dahliae,as well as the intricate interplay between cotton and this fungus.Moreover,we delve into the future prospects of cutting-edge research on cotton Verticillium wilt,aiming to proffer valuable insights for the effective management of this devastating fungus.
基金supported by the following grants,Earmarked fund for CARS-10-Sweet potato,High-quality development of agriculture“5511”collaborative innovation project(XTCXGC2021005)Natural Science Foundation of Fujian province(2021J01495)+1 种基金Basic Scientific Research Special Project for Fujian Provincial Public Research Institutes(2021R1031008)Science and Technology Innovation Team of Fujian Academy of Agricultural Sciences(CXTD2021012-1).
文摘Vascular wilt caused by Fusarium oxysporum f.sp.batatas(Fob)is a devastating disease threatening global sweet potato production.To elucidate Fob’s pathogenicitymechanisms and informeffective control strategies,we generated a green fluorescent protein(GFP)-tagged Fob strain to track infection dynamics in sweet potato susceptible cultivar Xinzhonghua and resistant cultivar Xiangshu75-55,respectively.Through cytological observation,we found in the susceptible Xinzhonghua,Fob predominantly colonized stem villi,injured root growth points,and directly invaded vascular bundles through stemwounds.Spore germination peaked at 2-3 h post-inoculation(hpi),followed by cyclical mycelial expansion and sporulation within vascular tissues with sustaining infection.In contrast,the resistant Xiangshu75-55 exhibited strong suppression of Fob:spores rarely germinated in vascular bundles or on trichomes by 3 hpi,and mature hyphae were absent in stems at 24 hpi.Quantitative reverse transcription PCR(qRT-PCR)confirmed significantly higher Fob biomass in Xinzhonghua than in Xiangshu75-55 by 16 hpi.Additionally,transcriptional profiling revealed distinct pathogen-host interactions during the compatible and incompatible reactions.In Xinzhonghua,Fob virulence genes FobPGX1,FobICL1,FobCTF2,FobFUB5 and FobFUB6 were upregulated within 16 hpi.Conversely,host defense genes IbMAPKK9,IbWRKY61,IbWRKY75,IbSWEET10,IbBBX24 and IbPIF4 were activated in Xiangshu75-55 during the same period.This study provides spatiotemporal cytological and molecular insights into Fob pathogenicity and host resistance,offering a foundation for early disease detection and improved Fusarium wilt management in sweet potato.
基金This work was supported by the National Natural Science Foundation of China(NSFC)(No.30070521 and 30270840).
文摘Bacterial wilt （BW） caused by Ralstonia solanacearum is an important constraint to peanut （Arachis hypogaea L.） production in several Asian and African countries, and planting BW-resistant cultivars is the most feasible method for controlling the disease. Although several BW-resistant peanut germplasm accessions have been identified, the genetic diversity among these has not been properly investigated, which has impeded efficient utilization. In this study, the genetic relationships of 31 peanut genotypes with various levels of resistance to BW were assessed based on SSR and AFLP analyses. Twenty-nine of 78 SSR primers and 32 of 126 AFLP primer combinations employed in this study were polymorphic amongst the peanut genotypes tested. The SSR primers amplified 91 polymorphic loci in total with an average of 3.14 alleles per primer, and the AFLP primers amplified 72 polymorphic loci in total with an average of 2.25 alleles per primer. Four SSR primers （14H06, 7G02, 3A8, 16C6） and one AFLP primer （P1M62） were found to be most efficient in detecting diversity. The genetic distance between pairs of genotypes ranged from 0.12 to 0.94 with an average of 0.53 in the SSR data and from 0.06 to 0.57 with an average of 0.25 in the AFLP data. The SSR-based estimates of the genetic distance were generally larger than that based on the AFLP data. The genotypes belonging to subsp, fastigiata possessed wider diversity than that of subsp, hypogaea. The clustering of genotypes based on the SSR and AFLP data were similar but the SSR clustering was more consistent with morphological classification ofA. hypogaea. Optimum diverse genotypes of both subsp, hypogaea and subsp.fastigiata can be recommended based on this analysis for developing mapping populations and breeding for high yielding and resistant cultivars.
基金Supported by the Fund of Science and Technology in GuangXi Zhuang Autonomous Region(0009018)~~
文摘[Objective] The aim of this study was to breed new strains which have higher inhibitory effects on the pathogens of watermelon fusarium wilt.[Method] The endophytic Bacillus subtilis B47 strain was obtained from tomato stems by UV mutagenesis for two consecutive times,then genetic stability as well as physiological and biochemical properties of mutant strains were studied.[Result] The antibacterial activity of all the three mutant strains F303,F304 and F305 was higher than that of B74 strain.After subculture of 10 successive generations,the antibacterial activity of all the three mutant strains for the pathogens of watermelon fusarium wilt decreased,but the antibacterial activity of F305 strain decreased the least,indicating its best genetic stability among the tested strains.The antibacterial circle diameter of F305 strain was 5 mm larger than that of wild strain B47 under the same condition.The mutant strain F305 was in logarithmic growth phase within 36 h and in stationary phase within 36-96 h,while its optimum growth temperature was 35 ℃.F305 strain could grow in sodium salt with the concentration of 1%-10%,but it grew best at the concentration of 1%.Physiological and biochemical responses of F305 strain were in accordance with those of wild strain B47.[Conclusion] This study lays the foundation for the factorial production of antagonistic substance by B47 strain and new methods of preventing from the pathogens watermelon fusarium wilt.
基金Supported by Scientific Research and Development Fund of Hefei University of Technology(070602F)Research Funding Project for Young Teachers in Colleges of Anhui Province(2008JQ1009)~~
文摘[Objective] The aim of this study was to screen bacteria strains with stable antagonistic effect against watermelon fusarium wilt from soil and investigate the biological control of watermelon fusarium wilt by applying the antagonistic bacteria strains into soil.[Method] Actinomycete strains,fluorescent bacteria strains and bacillus strains were isolated from soil samples by the dilution-plate method,then its resistance was screened respectively by the improved confront culture method after colonies were purified.Finally,bacteria strains with better antagonistic effect were identified.[Result] 29 bacteria strains with stable antagonistic effect against watermelon fusarium wilt were screened from 39 soil samples,which contained 15 fluorescent bacteria strains,5 bacillus strains and 9 actinomycete strains.Furthermore,three antagonistic bacteria strains of FM2,FM3 and FM4 with the strongest antagonism were identified primarily.[Conclusion] According to cultural characteristics,morphological observation,biochemical and physiological tests,FM2 belongs to bacillus subtilis,while FM3 and FM4 belong to micrococcus.
基金Supported by Breeding Key Project of Sichuan Province(2006-yzgg-25)~~
文摘[Objective] Aimed to lay a scientific foundation for eggplant resistant breeding, the resistant field identification of eggplant resources to Verticillium wilt was carried out. [Method] Test materials were 35 eggplant resources, which were collected from various places in Sichuan province since 2001. These eggplant resources were made resistance evaluation to Verticillium wilt by watering injured roots with Verticillium dahliae spore suspension. [Result] The eggplant resources had good resistance to Verticillium wilt. Among 35 evaluated resources, 10 resources like 242-9OP, 41-1,35-R5, etc. were resistant, while 23 resources like 68 ×47, Zizaoqie, 32-1, etc. were tolerant. Some resistant resources like 41-1,35-R5, 47-R1, etc. had been transferred into new resistant varieties like EF9910, etc. by breeding institutes. [Conclusion] The resistant level and future resistance breeding direction of eggplant resources in Sichuan were clarified by the resistance evaluation of eggplant resources so as to provide a technical guidance for eggplant resistance breeding.
基金Supported by National"973" Project(2011CB100400)~~
文摘[Objectives] This paper aims to explore the possibility to intercrop garlic with pomegranate tree to control pomegranate wilt.[Methods] Root exudates of garlic is cultivated in 1/5 concentration of MS solution and distilled water is examined in lab to test their effect to growth of mycelia of pomegranate wilt pathogen（Ceratocystis fimbriata）and multiplication of Bacillus subtilis.[Results] The result shows that garlic root exudates whatever cultivated in MS solution or distilled water could not inhibit or promote mycelia growth of C.fimbriata.However,garlic root exudates cultivated in both methods effectively promote multiplication of B.subtilis.[Conclusions] It is suggested that intercropping garlic with pomegranate tree by combining application B.subtilis could be a promising way to prevent pomegranate wilt spread in practice.
