Some stress response-related genes have been identified in Ustilaginoidea virens,but it is not clear whether and how defects of stress responses affect the pathogenesis processes of U.virens.In this study,we identifie...Some stress response-related genes have been identified in Ustilaginoidea virens,but it is not clear whether and how defects of stress responses affect the pathogenesis processes of U.virens.In this study,we identified a general stress response factor UvWHI2 as a homolog of Saccharomyces cerevisiae Whi2 in U.virens.The relative expression level of Uv Whi2 was significantly up-regulated during infection,suggesting that UvWHI2 may be involved in pathogenesis.Furthermore,knockout of Uv Whi2 showed decreased mycelial growth,increased conidiation in the potato sucrose medium and a defect in pathogenicity.In addition,the RNA-Seq and phenotypic analysis showed that UvWHI2 was involved in response to oxidative,hyperosmotic,cell wall stress and nutrient limitation.Further studies revealed that the defects of stress responses of the?Uvwhi2 mutant affected the formation of secondary spores on the nutrient limited surface and the rice surface,resulting in a significant reduction of pathogenicity of U.virens.Our results suggest that UvWHI2 is necessary for fungal growth,stress responses and the formation of secondary spores in U.virens.In addition,the defects of stress responses can affect the formation of secondary spores on the rice surface,and then compromise the pathogenicity of U.virens.展开更多
Whisper Whisky is an oak-and-leather lounge that outwardly manifests sophistication,stocked with cigars and about 100 whiskies so far,with many more on the way.But where it succeeds in appearance,it falls a bit short ...Whisper Whisky is an oak-and-leather lounge that outwardly manifests sophistication,stocked with cigars and about 100 whiskies so far,with many more on the way.But where it succeeds in appearance,it falls a bit short in substance.展开更多
Everyone loves cookies,and Shanghai certainly has some great specimens.But which is the best?From artisanal handmade to mass-produced cookies,we blindfolded four That’s cookie lovers in search of unbiased impressions...Everyone loves cookies,and Shanghai certainly has some great specimens.But which is the best?From artisanal handmade to mass-produced cookies,we blindfolded four That’s cookie lovers in search of unbiased impressions of 10 cookies found in Shanghai.展开更多
基金funded by the Zhejiang Provincial Natural Science Foundation of China(Grant No.LQ19C140004)the Key Research and Development Project of Zhejiang Province,China(Grant No.2019C02018)Key Research and Development Project of China National Rice Research Institute(Grant No.CNRRI-2020-04)。
文摘Some stress response-related genes have been identified in Ustilaginoidea virens,but it is not clear whether and how defects of stress responses affect the pathogenesis processes of U.virens.In this study,we identified a general stress response factor UvWHI2 as a homolog of Saccharomyces cerevisiae Whi2 in U.virens.The relative expression level of Uv Whi2 was significantly up-regulated during infection,suggesting that UvWHI2 may be involved in pathogenesis.Furthermore,knockout of Uv Whi2 showed decreased mycelial growth,increased conidiation in the potato sucrose medium and a defect in pathogenicity.In addition,the RNA-Seq and phenotypic analysis showed that UvWHI2 was involved in response to oxidative,hyperosmotic,cell wall stress and nutrient limitation.Further studies revealed that the defects of stress responses of the?Uvwhi2 mutant affected the formation of secondary spores on the nutrient limited surface and the rice surface,resulting in a significant reduction of pathogenicity of U.virens.Our results suggest that UvWHI2 is necessary for fungal growth,stress responses and the formation of secondary spores in U.virens.In addition,the defects of stress responses can affect the formation of secondary spores on the rice surface,and then compromise the pathogenicity of U.virens.
文摘Whisper Whisky is an oak-and-leather lounge that outwardly manifests sophistication,stocked with cigars and about 100 whiskies so far,with many more on the way.But where it succeeds in appearance,it falls a bit short in substance.
文摘Everyone loves cookies,and Shanghai certainly has some great specimens.But which is the best?From artisanal handmade to mass-produced cookies,we blindfolded four That’s cookie lovers in search of unbiased impressions of 10 cookies found in Shanghai.
