Cucurbitaceae is one of the most important plant families distributed worldwide.Transcription factors(TFs)regulate plant growth at the transcription level.Here,we performed a systematic analysis of 42641 TFs from 63 f...Cucurbitaceae is one of the most important plant families distributed worldwide.Transcription factors(TFs)regulate plant growth at the transcription level.Here,we performed a systematic analysis of 42641 TFs from 63 families in 14 Cucurbitaceae and 10 non-cucurbit species.Whole-genome duplication(WGD)was the dominant event type in almost all Cucurbitaceae plants.The TF families were divided into 1210 orthogroups(OGs),of which,112 were unique to Cucurbitaceae.Although the loss of several gene families was detected in Cucurbitaceae,the gene families expanded in five species that experienced a WGD event comparing with grape.Our findings revealed that the recent WGD events that had occurred in Cucurbitaceae played important roles in the expansion of most TF families.The functional enrichment analysis of the genes that significantly expanded or contracted uncovered five gene families,AUX/IAA,NAC,NBS,HB,and NF-YB.Finally,we conducted a comprehensive analysis of the TCP gene family and identified 16 tendril-related(TEN)genes in 11 Cucurbitaceae species.Interestingly,the characteristic sequence changed from CNNFYFP to CNNFYLP in the TEN gene(Bhi06M000087)of Benincasa hispida.Furthermore,we identified a new characteristic sequence,YNN,which could be used for TEN gene exploitation in Cucurbitaceae.In conclusion,this study will serve as a reference for studying the relationship between gene family evolution and genome duplication.Moreover,it will provide rich genetic resources for functional Cucurbitaceae studies in the future.展开更多
为探讨1.4%甲磺·五氟·异噁颗粒剂在移栽稻田的应用前景,2014—2015年开展田间应用技术研究。结果表明,施药后56 d,1.4%甲磺·五氟·异噁颗粒剂231~588 g a.i./hm^2对移栽稻田杂草稗[Echinochloacrusgali(L.)Beauv.]...为探讨1.4%甲磺·五氟·异噁颗粒剂在移栽稻田的应用前景,2014—2015年开展田间应用技术研究。结果表明,施药后56 d,1.4%甲磺·五氟·异噁颗粒剂231~588 g a.i./hm^2对移栽稻田杂草稗[Echinochloacrusgali(L.)Beauv.]、异型莎草(Cyperus difformis L.)、水苋菜属杂草(Ammannia spp.)和鸭舌草[Monochoria vaginali(Burm.f.)Presl ex Kunth]的株数防效分别为95.40%~100.00%、94.30%~100.00%、92.96%~100.00%和96.11%~100.00%,总草株数防效达94.75%~100.00%,总草鲜质量防效达96.24%~100.00%;总草株数防效和总草鲜质量防效均显著优于各个对照药剂。在推荐剂量231~294 g a.i./hm^2下1.4%甲磺·五氟·异噁颗粒剂对水稻茎孽数、株高、地上部鲜质量、根长和地下部鲜质量等生长指标无不良影响。水稻移栽后5~7 d一次撒施即可有效解决移栽稻田整个生长季杂草危害,应用推广前景广阔。展开更多
Olfactory receptors(ORs)play a key role in the prime sensorial perception,being highly relevant for intra/interspecific interactions.ORs are a subgroup of G-protein coupled receptors that exhibit highly complex subgen...Olfactory receptors(ORs)play a key role in the prime sensorial perception,being highly relevant for intra/interspecific interactions.ORs are a subgroup of G-protein coupled receptors that exhibit highly complex subgenomes in vertebrates.However,OR repertoires remain poorly studied in fish lineages,precluding finely retracing their origin,evolution,and diversification,especially in the most basal groups.Here,we conduct an exhaustive gene screening upon 43 high-quality fish genomes exhibiting varied gene repertoires(2e583 genes).While the early vertebrates performed gas exchange through gills,we hypothesize that the emergence of new breathing structures(swim bladder and paired lungs)in early osteichthyans may be associated with expansions in the ORs gene families sensitive to airborne molecules.Additionally,we verify that the OR repertoire of moderns actinopterygians has not increased as expected following a whole genome duplication,likely due to regulatory mechanisms compensating the gene load excess.Finally,we identify 25 distinct OR families,allowing us to propose an updated universal nomenclature for the fish ORs.展开更多
The C-repeat binding factors/dehydrationresponsive element binding protein 1 s(CBFs/DREB1 s)have been identified as major regulators of cold acclimation in many angiosperm plants.However,their origin and evolutionary ...The C-repeat binding factors/dehydrationresponsive element binding protein 1 s(CBFs/DREB1 s)have been identified as major regulators of cold acclimation in many angiosperm plants.However,their origin and evolutionary process associated to cold responsiveness are still lacking.By integrating multi-omics data of genomes,transcriptomes,and CBFs/DREB1 s genome-wide binding profiles,we unveil the origin and evolution of CBFs/DREB1 s and their regulatory network.Gene collinearity and phylogeny analyses show that CBF/DREB1 is an innovation evolved from tandem duplication-derived DREBⅢgene.A subsequent event of e-whole genome duplication led to two CBF/DREB1 archetypes(CladesⅠandⅡ)in ancient angiosperms.In contrast to cold-insensitivity of Clade I and their parent DREBⅢgenes,CladeⅡevolved a further innovation in cold-sensitive response and was stepwise expanded in eudicots and monocots by independent duplications.In geological time,the duplication events were mainly enriched around the Cretaceous-Paleogene(K-Pg)boundary and/or in the Late Cenozoic Ice Age,when the global average temperature significantly decreased.Consequently,the duplicated CBF/DREB1 genes contributed to the rewiring of CBFs/DREB1 s-regulatory network for cold tolerance.Altogether,our results highlight an origin and convergent evolution of CBFs/DREB1 s and their regulatory network probably for angiosperms adaptation to global cooling.展开更多
Polyploidization via whole-genome duplications （WGD） is a common phenomenon in organisms. However, investigations into this phenomenon differ greatly between plants and animals. Recent research on polyploid plants i...Polyploidization via whole-genome duplications （WGD） is a common phenomenon in organisms. However, investigations into this phenomenon differ greatly between plants and animals. Recent research on polyploid plants illustrates the immediate changes that follow WGDs and the mechanisms behind in both genetic and epigenetic consequences. Unfortunately, equivalent questions remain to be explored in animals. Enlightened by botanical research, the study of polyploidization in vertebrates involves the identification of model animals and the establishment of strategies. Here we review and compare the research on plants and vertebrates while considering intrageneric or intraspecific variation in genome size. Suitable research methods on recently established poly- ploidy systems could provide important clues for under- standing what happens after WGDs in vertebrates. The approach yields insights into survival and the rarity of polyploidization in vertebrates. The species of Carassius and the allopolyploid system of goldfish × common carp hybridization appear to be suitable models for unraveling the evolution and adaptation of polyploid vertebrates.展开更多
基金supported by the Natural Science Foundation of Hebei(Grant No.C2021209005)National Natural Science Foundation of China(Grant No.32172583)+1 种基金the Natural Science Foundation for Distinguished Young Scholar of Hebei Province(Grant No.C2022209010)the China Postdoctoral Science Foundation(Grant Nos.2020M673188,2021T140097).
文摘Cucurbitaceae is one of the most important plant families distributed worldwide.Transcription factors(TFs)regulate plant growth at the transcription level.Here,we performed a systematic analysis of 42641 TFs from 63 families in 14 Cucurbitaceae and 10 non-cucurbit species.Whole-genome duplication(WGD)was the dominant event type in almost all Cucurbitaceae plants.The TF families were divided into 1210 orthogroups(OGs),of which,112 were unique to Cucurbitaceae.Although the loss of several gene families was detected in Cucurbitaceae,the gene families expanded in five species that experienced a WGD event comparing with grape.Our findings revealed that the recent WGD events that had occurred in Cucurbitaceae played important roles in the expansion of most TF families.The functional enrichment analysis of the genes that significantly expanded or contracted uncovered five gene families,AUX/IAA,NAC,NBS,HB,and NF-YB.Finally,we conducted a comprehensive analysis of the TCP gene family and identified 16 tendril-related(TEN)genes in 11 Cucurbitaceae species.Interestingly,the characteristic sequence changed from CNNFYFP to CNNFYLP in the TEN gene(Bhi06M000087)of Benincasa hispida.Furthermore,we identified a new characteristic sequence,YNN,which could be used for TEN gene exploitation in Cucurbitaceae.In conclusion,this study will serve as a reference for studying the relationship between gene family evolution and genome duplication.Moreover,it will provide rich genetic resources for functional Cucurbitaceae studies in the future.
文摘为探讨1.4%甲磺·五氟·异噁颗粒剂在移栽稻田的应用前景,2014—2015年开展田间应用技术研究。结果表明,施药后56 d,1.4%甲磺·五氟·异噁颗粒剂231~588 g a.i./hm^2对移栽稻田杂草稗[Echinochloacrusgali(L.)Beauv.]、异型莎草(Cyperus difformis L.)、水苋菜属杂草(Ammannia spp.)和鸭舌草[Monochoria vaginali(Burm.f.)Presl ex Kunth]的株数防效分别为95.40%~100.00%、94.30%~100.00%、92.96%~100.00%和96.11%~100.00%,总草株数防效达94.75%~100.00%,总草鲜质量防效达96.24%~100.00%;总草株数防效和总草鲜质量防效均显著优于各个对照药剂。在推荐剂量231~294 g a.i./hm^2下1.4%甲磺·五氟·异噁颗粒剂对水稻茎孽数、株高、地上部鲜质量、根长和地下部鲜质量等生长指标无不良影响。水稻移栽后5~7 d一次撒施即可有效解决移栽稻田整个生长季杂草危害,应用推广前景广阔。
基金was supported by a PhD grant from "Fundagao para a Ciencia e a Tecnologia"(FCT)(L.S.:SFRH/BD/134565/2017,COVID/BD/151995/2021,T.M.:SFRH/BD/112458/2015supported by a PhD grant from“Fundação para a CiêL.R.:SFRH/BD/142322/2018)+3 种基金A.A.was partially supported by the Strategic Funding UIDB/04423/2020 and UIDP/04423/2020 through national funds provided by FCT and the European Regional Development Fund(ERDF)in the framework of the program PT 2020the European Structural and Investment Funds(ESIF)through the Competitiveness and Internationalization Operational Program e COMPETE 2020 through the FCT project PTDC/CTA-AMB/31774/2017(POCI-01-0145-FEDER/031774/2017)the projects Ocean3R(NORTE-01-0145-FEDER-000064)Atlantida(NORTE-01-0145-FEDER-000040).
文摘Olfactory receptors(ORs)play a key role in the prime sensorial perception,being highly relevant for intra/interspecific interactions.ORs are a subgroup of G-protein coupled receptors that exhibit highly complex subgenomes in vertebrates.However,OR repertoires remain poorly studied in fish lineages,precluding finely retracing their origin,evolution,and diversification,especially in the most basal groups.Here,we conduct an exhaustive gene screening upon 43 high-quality fish genomes exhibiting varied gene repertoires(2e583 genes).While the early vertebrates performed gas exchange through gills,we hypothesize that the emergence of new breathing structures(swim bladder and paired lungs)in early osteichthyans may be associated with expansions in the ORs gene families sensitive to airborne molecules.Additionally,we verify that the OR repertoire of moderns actinopterygians has not increased as expected following a whole genome duplication,likely due to regulatory mechanisms compensating the gene load excess.Finally,we identify 25 distinct OR families,allowing us to propose an updated universal nomenclature for the fish ORs.
基金supported by National Key R&D Program of China(2018YFD1000604)National Natural Science Foundation of China(31871233)Natural Science Foundation of Zhejiang Province(LY22C160005)。
文摘The C-repeat binding factors/dehydrationresponsive element binding protein 1 s(CBFs/DREB1 s)have been identified as major regulators of cold acclimation in many angiosperm plants.However,their origin and evolutionary process associated to cold responsiveness are still lacking.By integrating multi-omics data of genomes,transcriptomes,and CBFs/DREB1 s genome-wide binding profiles,we unveil the origin and evolution of CBFs/DREB1 s and their regulatory network.Gene collinearity and phylogeny analyses show that CBF/DREB1 is an innovation evolved from tandem duplication-derived DREBⅢgene.A subsequent event of e-whole genome duplication led to two CBF/DREB1 archetypes(CladesⅠandⅡ)in ancient angiosperms.In contrast to cold-insensitivity of Clade I and their parent DREBⅢgenes,CladeⅡevolved a further innovation in cold-sensitive response and was stepwise expanded in eudicots and monocots by independent duplications.In geological time,the duplication events were mainly enriched around the Cretaceous-Paleogene(K-Pg)boundary and/or in the Late Cenozoic Ice Age,when the global average temperature significantly decreased.Consequently,the duplicated CBF/DREB1 genes contributed to the rewiring of CBFs/DREB1 s-regulatory network for cold tolerance.Altogether,our results highlight an origin and convergent evolution of CBFs/DREB1 s and their regulatory network probably for angiosperms adaptation to global cooling.
基金supported by the National Natural Science Foundation of China(91331105,31360514)
文摘Polyploidization via whole-genome duplications （WGD） is a common phenomenon in organisms. However, investigations into this phenomenon differ greatly between plants and animals. Recent research on polyploid plants illustrates the immediate changes that follow WGDs and the mechanisms behind in both genetic and epigenetic consequences. Unfortunately, equivalent questions remain to be explored in animals. Enlightened by botanical research, the study of polyploidization in vertebrates involves the identification of model animals and the establishment of strategies. Here we review and compare the research on plants and vertebrates while considering intrageneric or intraspecific variation in genome size. Suitable research methods on recently established poly- ploidy systems could provide important clues for under- standing what happens after WGDs in vertebrates. The approach yields insights into survival and the rarity of polyploidization in vertebrates. The species of Carassius and the allopolyploid system of goldfish × common carp hybridization appear to be suitable models for unraveling the evolution and adaptation of polyploid vertebrates.
