Regulation of seed size is a key strategy for improving crop yield and is also a basic biological question.However,the molecular mechanisms by which plants determine their seed size remain elusive.Here,we report that ...Regulation of seed size is a key strategy for improving crop yield and is also a basic biological question.However,the molecular mechanisms by which plants determine their seed size remain elusive.Here,we report that the GW2-WG1-OsbZIP47 regulatory module controls grain width and weight in rice.WG1,which encodes a glutaredoxin protein,promotes grain growth by increasing cell proliferation.Interestingly,WG1 interacts with the transcription factor OsbZIP47 and represses its transcriptional activity by associating with the transcriptional co-repressor ASP1,indicating that WG1 may act as an adaptor protein to recruit the transcriptional co-repressor.In contrary,OsbZIP47 restricts grain growth by decreasing cell proliferation.Further studies reveal that the E3 ubiquitin ligase GW2 ubiquitinates WG1 and targets it for degradation.Genetic analyses confirm that GW2,WG1,and OsbZIP47 function in a comm on pathway to control grain growth.Taken together,ourfindi ngs reveal a genetic and molecular framework for the control of grain size and weight by the GW2-WG1-OsbZIP47 regulatory module,providing new targets for improving seed size and weight in crops.展开更多
基金supported by grants from the National Natural Science Foundation of China(3181101602 to Y.L,91735302 to Y.L.,3187080209 to N.L.,91735304 to Q.Q.,31571742 to D.W.)the strategic priority research program of the Chinese Academy of Sciences(XDB27010102)+3 种基金the National Basic Researoh Program of China(2018YFD1000706-10 to R.X.,2016YFD0100501 to N.L.,2016YFD0100402 to P.D.,2016YFD0100401 to D.W.,2017YFD0101701 to R.X.)the National Ten-Thousand Talents Program of China to Y.LI.the Youth innovation promotion association CAS to P.D.(2019102)the National Special Project(2016ZX08009003-003).
文摘Regulation of seed size is a key strategy for improving crop yield and is also a basic biological question.However,the molecular mechanisms by which plants determine their seed size remain elusive.Here,we report that the GW2-WG1-OsbZIP47 regulatory module controls grain width and weight in rice.WG1,which encodes a glutaredoxin protein,promotes grain growth by increasing cell proliferation.Interestingly,WG1 interacts with the transcription factor OsbZIP47 and represses its transcriptional activity by associating with the transcriptional co-repressor ASP1,indicating that WG1 may act as an adaptor protein to recruit the transcriptional co-repressor.In contrary,OsbZIP47 restricts grain growth by decreasing cell proliferation.Further studies reveal that the E3 ubiquitin ligase GW2 ubiquitinates WG1 and targets it for degradation.Genetic analyses confirm that GW2,WG1,and OsbZIP47 function in a comm on pathway to control grain growth.Taken together,ourfindi ngs reveal a genetic and molecular framework for the control of grain size and weight by the GW2-WG1-OsbZIP47 regulatory module,providing new targets for improving seed size and weight in crops.
