Glioma is a general malignant tumor with a dismal prognosis.Long noncoding RNAs(lncRNAs)have been implicated in the initiation and processes of tumors.An investigation of the GEPIA database revealed that long noncodin...Glioma is a general malignant tumor with a dismal prognosis.Long noncoding RNAs(lncRNAs)have been implicated in the initiation and processes of tumors.An investigation of the GEPIA database revealed that long noncoding RNA WEE2 antisense RNA 1(WEE2-AS1)is upregulated in glioma tissues compared to normal brain tissues,and validation with quantitative real-time polymerase chain reaction(qRT–PCR)revealed that WEE2-AS1 expression was consistent with the database prediction.Fluorescence in situ hybridization(FISH)assays revealed that WEE2-AS1 was localized primarily in the cytoplasm.Clone formation experiment and EDU assay were used to detect cell proliferation ability,and Transwell assay was used to detect cell migration and invasion ability,Western-blot assay and immunofluorescence were used to determine TPM3 protein level.Functional experiments revealed that the downregulation of WEE2-AS1 impeded cell proliferation,migration,and invasion in glioma cell lines.Furthermore,downregulation of WEE2-AS1 suppressed tumor growth in vivo.Bioinformatics predictions and integrated experiments indicated that WEE2-AS1 promoted tropomyosin 3(TPM3)expression by sponging miR-29b-2-5p.A dual-luciferase reporter assay was conducted to uncover the binding of WEE2-AS1 and miR-29b-2-5p and that of miR-29b-2-5p and TPM3.Additionally,a series of rescue assays showed that WEE2-AS1 promotes proliferation,migration,and invasion by targeting miR-29b-2-5p to regulate TPM3 expression.Ultimately,the results of this study indicate that WEE2-AS1 plays an oncogenic role in glioma and may promote further investigations of the diagnostic and prognostic value of WEE2-AS1 in glioma.展开更多
基金supported by Jiangsu Commission of Health(M2020046).
文摘Glioma is a general malignant tumor with a dismal prognosis.Long noncoding RNAs(lncRNAs)have been implicated in the initiation and processes of tumors.An investigation of the GEPIA database revealed that long noncoding RNA WEE2 antisense RNA 1(WEE2-AS1)is upregulated in glioma tissues compared to normal brain tissues,and validation with quantitative real-time polymerase chain reaction(qRT–PCR)revealed that WEE2-AS1 expression was consistent with the database prediction.Fluorescence in situ hybridization(FISH)assays revealed that WEE2-AS1 was localized primarily in the cytoplasm.Clone formation experiment and EDU assay were used to detect cell proliferation ability,and Transwell assay was used to detect cell migration and invasion ability,Western-blot assay and immunofluorescence were used to determine TPM3 protein level.Functional experiments revealed that the downregulation of WEE2-AS1 impeded cell proliferation,migration,and invasion in glioma cell lines.Furthermore,downregulation of WEE2-AS1 suppressed tumor growth in vivo.Bioinformatics predictions and integrated experiments indicated that WEE2-AS1 promoted tropomyosin 3(TPM3)expression by sponging miR-29b-2-5p.A dual-luciferase reporter assay was conducted to uncover the binding of WEE2-AS1 and miR-29b-2-5p and that of miR-29b-2-5p and TPM3.Additionally,a series of rescue assays showed that WEE2-AS1 promotes proliferation,migration,and invasion by targeting miR-29b-2-5p to regulate TPM3 expression.Ultimately,the results of this study indicate that WEE2-AS1 plays an oncogenic role in glioma and may promote further investigations of the diagnostic and prognostic value of WEE2-AS1 in glioma.
