BACKGROUND Periodontitis is an inflammatory disease caused by the host’s immune response and various interactions between pathogens,which lead to the loss of connective tissue and bone.In recent years,mesenchymal ste...BACKGROUND Periodontitis is an inflammatory disease caused by the host’s immune response and various interactions between pathogens,which lead to the loss of connective tissue and bone.In recent years,mesenchymal stem cell(SC)transplantation technology has become a research hotspot,which can form periodontal ligament,cementum,and alveolar bone through proliferation and differentiation.AIM To elucidate the regulatory effects of WD repeat-containing protein 36(WDR36)on the senescence,migration,and osteogenic differentiation of periodontal ligament SCs(PDLSCs).METHODS The migration and chemotaxis of PDLSCs were detected by the scratch-wound migration test and transwell chemotaxis test.Alkaline phosphatase(ALP)activity,Alizarin red staining,calcium content,and real-time reverse transcription polymerase chain reaction(RT-qPCR)of key transcription factors were used to detect the osteogenic differentiation function of PDLSCs.Cell senescence was determined by senescence-associatedβ-galactosidase staining.RESULTS The 24-hour and 48-hour scratch-wound migration test and 48-hour transwell chemotaxis test showed that overexpression of WDR36 inhibited the migration/chemotaxis of PDLSCs.Simultaneously,WDR36 depletion promoted the migration/chemotaxis of PDLSCs.The results of ALP activity,Alizarin red staining,calcium content,and RTqPCR showed that overexpression of WDR36 inhibited the osteogenic differentiation of PDLSCs,and WDR36 depletion promoted the osteogenic differentiation of PDLSCs.Senescence-associatedβ-galactosidase staining showed that 0.1μg/mL icariin(ICA)and overexpression of WDR36 inhibited the senescence of PDLSCs,and WDR36 depletion promoted the osteogenic differentiation of PDLSCs.CONCLUSION WDR36 inhibits the migration and chemotaxis,osteogenic differentiation,and senescence of PDLSCs;0.1μg/mL ICA inhibits the senescence of PDLSCs.Therefore,WDR36 might serve as a target for periodontal tissue regeneration and the treatment of periodontitis.展开更多
WD蛋白亚家族成员包含WSB1 (WD Repeat and SOCS Box Containing 1)和WSB2 (WD Repeat and SOCS Box Containing 2),它们的共同结构是WD40重复序列和C端一个细胞因子信号传导抑制因子(SOCS)盒。WD蛋白亚家族在肝细胞癌、前列腺癌、胰腺...WD蛋白亚家族成员包含WSB1 (WD Repeat and SOCS Box Containing 1)和WSB2 (WD Repeat and SOCS Box Containing 2),它们的共同结构是WD40重复序列和C端一个细胞因子信号传导抑制因子(SOCS)盒。WD蛋白亚家族在肝细胞癌、前列腺癌、胰腺癌、神经母细胞瘤、乳腺癌等多种人体肿瘤中呈现异常表达,参与肿瘤细胞的增殖、转移、凋亡、治疗耐药等过程,并影响肿瘤的预后。本文主要就WD蛋白亚家族在肿瘤中的研究进展进行综述,以期为进一步研究其生物学功能、分子机制及临床研究提供理论参考。WD protein subfamily members include WSB1 (WD Repeat and SOCS Box Containing 1) and WSB2 (WD Repeat and SOCS Box Containing 2), which share a common structure consisting of a WD40 repeat sequence and a C-terminal SOCS (cellular factor signaling inhibitor) box. WD protein subfamily members exhibit abnormal expression in various human tumors, including hepatocellular carcinoma, prostate cancer, pancreatic cancer, neuroblastoma, and breast cancer, and participate in various processes of tumor cell proliferation, metastasis, apoptosis, and drug resistance. This review mainly summarizes the research progress of the WD protein subfamily in tumors.展开更多
文摘BACKGROUND Periodontitis is an inflammatory disease caused by the host’s immune response and various interactions between pathogens,which lead to the loss of connective tissue and bone.In recent years,mesenchymal stem cell(SC)transplantation technology has become a research hotspot,which can form periodontal ligament,cementum,and alveolar bone through proliferation and differentiation.AIM To elucidate the regulatory effects of WD repeat-containing protein 36(WDR36)on the senescence,migration,and osteogenic differentiation of periodontal ligament SCs(PDLSCs).METHODS The migration and chemotaxis of PDLSCs were detected by the scratch-wound migration test and transwell chemotaxis test.Alkaline phosphatase(ALP)activity,Alizarin red staining,calcium content,and real-time reverse transcription polymerase chain reaction(RT-qPCR)of key transcription factors were used to detect the osteogenic differentiation function of PDLSCs.Cell senescence was determined by senescence-associatedβ-galactosidase staining.RESULTS The 24-hour and 48-hour scratch-wound migration test and 48-hour transwell chemotaxis test showed that overexpression of WDR36 inhibited the migration/chemotaxis of PDLSCs.Simultaneously,WDR36 depletion promoted the migration/chemotaxis of PDLSCs.The results of ALP activity,Alizarin red staining,calcium content,and RTqPCR showed that overexpression of WDR36 inhibited the osteogenic differentiation of PDLSCs,and WDR36 depletion promoted the osteogenic differentiation of PDLSCs.Senescence-associatedβ-galactosidase staining showed that 0.1μg/mL icariin(ICA)and overexpression of WDR36 inhibited the senescence of PDLSCs,and WDR36 depletion promoted the osteogenic differentiation of PDLSCs.CONCLUSION WDR36 inhibits the migration and chemotaxis,osteogenic differentiation,and senescence of PDLSCs;0.1μg/mL ICA inhibits the senescence of PDLSCs.Therefore,WDR36 might serve as a target for periodontal tissue regeneration and the treatment of periodontitis.
文摘WD蛋白亚家族成员包含WSB1 (WD Repeat and SOCS Box Containing 1)和WSB2 (WD Repeat and SOCS Box Containing 2),它们的共同结构是WD40重复序列和C端一个细胞因子信号传导抑制因子(SOCS)盒。WD蛋白亚家族在肝细胞癌、前列腺癌、胰腺癌、神经母细胞瘤、乳腺癌等多种人体肿瘤中呈现异常表达,参与肿瘤细胞的增殖、转移、凋亡、治疗耐药等过程,并影响肿瘤的预后。本文主要就WD蛋白亚家族在肿瘤中的研究进展进行综述,以期为进一步研究其生物学功能、分子机制及临床研究提供理论参考。WD protein subfamily members include WSB1 (WD Repeat and SOCS Box Containing 1) and WSB2 (WD Repeat and SOCS Box Containing 2), which share a common structure consisting of a WD40 repeat sequence and a C-terminal SOCS (cellular factor signaling inhibitor) box. WD protein subfamily members exhibit abnormal expression in various human tumors, including hepatocellular carcinoma, prostate cancer, pancreatic cancer, neuroblastoma, and breast cancer, and participate in various processes of tumor cell proliferation, metastasis, apoptosis, and drug resistance. This review mainly summarizes the research progress of the WD protein subfamily in tumors.
