Whitebacked planthopper (WBPH,Sogatella furcifera Horvath) is one ofthe most destructive insects for rice.The utilization of WBPH resistancegenes is always an efficient solution tothis problem. Besides five WBPHresist...Whitebacked planthopper (WBPH,Sogatella furcifera Horvath) is one ofthe most destructive insects for rice.The utilization of WBPH resistancegenes is always an efficient solution tothis problem. Besides five WBPHresistance genes registered, Wbph1,Wbph 2 , Wbph 3 , wbph 4 , andWbphS, classical segregation analysis展开更多
Whitebacked planthopper (WBPH) is a kind of long-distance migratory pest. Its occurring amount is closely related to the climate change of large sphere. In recent years many researches have reported that the climate o...Whitebacked planthopper (WBPH) is a kind of long-distance migratory pest. Its occurring amount is closely related to the climate change of large sphere. In recent years many researches have reported that the climate of large sphere was affected by the Pacific sea temperature (ST). We studied the long-range forecast of the occurring amount of WBPH by using data provided by State Meteorological Administration, China. Taking the highest amount per 100 hills in main injurious generation of WBPH on early rice in Ganzhou Prefecture, Jiangxi Province as the object of forecast (y), and dividing y into six grades,i.e. 1-6 grades means the sequence of 1,000,1,001-3,000, 3,001-5,000, 5,001-10,000,10,001-20,000, and over 20,000 heads in order. Three regions were selected from the 286 grid points in the northwest Pacific. Region A was the maritime space between Black Tide Region and California Cold Flow Region, 160°E-130°W,展开更多
Although CRISPR/Cas9 has been widely used in insect gene editing,the need for the microinjection of preblastoderm embryos can preclude the technique being used in insect species with eggs that are small,have hard shel...Although CRISPR/Cas9 has been widely used in insect gene editing,the need for the microinjection of preblastoderm embryos can preclude the technique being used in insect species with eggs that are small,have hard shells,and/or are difficult to collect and maintain outside of their normal environment.Such is the case with Sogatella furcifera,the white-backed planthopper(WBPH),a significant pest of Oryza sativa(rice)that oviposits inside rice stems.Egg extraction from the stem runs the risk of mechanical damage and hatching is heavily influenced by the micro-environment of the rice stem.To bypass these issues,we targeted embryos prior to oviposition via direct parental(DIPA)-CRISPR,in which Cas9 and single-guide RNAs(sgRNAs)for the WBPH eye pigment gene tryptophan 2,3-dioxygenase were injected into the hemocoel of adult females.Females at varying numbers of days posteclosion were evaluated to determine at what stage their oocyte might be most capable of taking up the gene-editing components.An evaluation of the offspring indicated that the highest G0 gene-edited efficacy(56.7%)occurred in females injected 2 d posteclosion,and that those mutations were heritably transmitted to the G1 generation.This study demonstrates the potential utility of DIPA-CRISPR for future gene-editing studies in non-model insect species and can facilitate the development of novel pest management applications.展开更多
文摘Whitebacked planthopper (WBPH) is a kind of long-distance migratory pest. Its occurring amount is closely related to the climate change of large sphere. In recent years many researches have reported that the climate of large sphere was affected by the Pacific sea temperature (ST). We studied the long-range forecast of the occurring amount of WBPH by using data provided by State Meteorological Administration, China. Taking the highest amount per 100 hills in main injurious generation of WBPH on early rice in Ganzhou Prefecture, Jiangxi Province as the object of forecast (y), and dividing y into six grades,i.e. 1-6 grades means the sequence of 1,000,1,001-3,000, 3,001-5,000, 5,001-10,000,10,001-20,000, and over 20,000 heads in order. Three regions were selected from the 286 grid points in the northwest Pacific. Region A was the maritime space between Black Tide Region and California Cold Flow Region, 160°E-130°W,
基金supported by:the National Natural Science Foundation of China(grants 32370527,for PH,and 32260671,for MH)the Scientific Research Foundation of Guizhou University of China(grant 2017-33,for MH)+3 种基金the Program of Talent Cultivation of Guizhou University(grant(2019)05,for PH)the Science and Technology Support of Guizhou province(grant QKH(2017)2956,for MH)the Program of Introducing Talents of Discipline to Universities of China(111 Program,D20023)the Frontiers Science Center for Asymmetric Synthesis and Medicinal Molecules,Department of Education,Guizhou Province(Qianjiaohe KY number(2020)004).
文摘Although CRISPR/Cas9 has been widely used in insect gene editing,the need for the microinjection of preblastoderm embryos can preclude the technique being used in insect species with eggs that are small,have hard shells,and/or are difficult to collect and maintain outside of their normal environment.Such is the case with Sogatella furcifera,the white-backed planthopper(WBPH),a significant pest of Oryza sativa(rice)that oviposits inside rice stems.Egg extraction from the stem runs the risk of mechanical damage and hatching is heavily influenced by the micro-environment of the rice stem.To bypass these issues,we targeted embryos prior to oviposition via direct parental(DIPA)-CRISPR,in which Cas9 and single-guide RNAs(sgRNAs)for the WBPH eye pigment gene tryptophan 2,3-dioxygenase were injected into the hemocoel of adult females.Females at varying numbers of days posteclosion were evaluated to determine at what stage their oocyte might be most capable of taking up the gene-editing components.An evaluation of the offspring indicated that the highest G0 gene-edited efficacy(56.7%)occurred in females injected 2 d posteclosion,and that those mutations were heritably transmitted to the G1 generation.This study demonstrates the potential utility of DIPA-CRISPR for future gene-editing studies in non-model insect species and can facilitate the development of novel pest management applications.
