目的:探究环氧化酶-2(cyclooxygenase-2,COX-2)、分泌型卷曲相关蛋白4(secreted frizzled-related protein 4,SFRP4)和WW域结合蛋白2(WW-domain binding protein 2,WBP2)在子宫腺肌病中的表达及其与子宫腺肌病临床特征的关系。方法:选取...目的:探究环氧化酶-2(cyclooxygenase-2,COX-2)、分泌型卷曲相关蛋白4(secreted frizzled-related protein 4,SFRP4)和WW域结合蛋白2(WW-domain binding protein 2,WBP2)在子宫腺肌病中的表达及其与子宫腺肌病临床特征的关系。方法:选取2019年1月-2020年6月在郑州大学第三附属医院确诊子宫腺肌病并进行手术切除子宫的患者40例,收集40例患者的异位内膜标本,统计患者的痛经、月经量及子宫大小等临床资料。采用免疫组化SP法分别检测COX-2、SFRP4和WBP2在子宫腺肌病异位病灶中的表达,分析其相互关系及其与子宫腺肌病临床特征的关系。结果:COX-2和SFRP4的表达呈正相关(r_(s)=0.533,P<0.01),COX-2和WBP2的表达呈正相关(r_(s)=0.544,P<0.01),WBP2和SFRP4的表达呈正相关(r_(s)=0.574,P<0.01)。无或轻度痛经及中度痛经组与重度痛经组COX-2和SFRP4低表达与高表达分布情况比较,差异均有统计学意义(P<0.05),而两组WBP2低表达与高表达分布情况比较,差异无统计学意义(P>0.05)。子宫体积正常组与子宫体积增大组COX-2、SFRP4及WBP2低表达与高表达分布情况比较,差异均无统计学意义(P>0.05)。月经量正常组与月经量增大组COX-2、SFRP4及WBP2低表达与高表达分布情况比较,差异均无统计学意义(P>0.05)。结论:COX-2、SFRP4和WBP2可能参与了子宫腺肌病的发生发展,COX-2可作为治疗子宫腺肌病潜在的分子靶点。展开更多
Sex-biased microRNAs(miRNAs)influence gonadal development in fish by directly targeting genes associated with estrogen production pathways.WW domain-binding protein 2(WBP2)functions as a crucial transcriptional co-act...Sex-biased microRNAs(miRNAs)influence gonadal development in fish by directly targeting genes associated with estrogen production pathways.WW domain-binding protein 2(WBP2)functions as a crucial transcriptional co-activator of the estrogen and progesterone receptors(PGR).This study investigates the direct modulation of a sex-biased miR-133b on wbp2 and its regulatory role in gonadal development in fish,the greater amberjack(Seriola dumerili).Using dual-luciferase reporter assays,we demonstrate that wbp2 is a direct target of miR-133b,with miR-133b-3p binding to the 3'untranslated region(3'UTR)of wbp2.In vitro,miR-133b mimic significantly downregulate wbp2 expression,while the miR-133b inhibitor increase wbp2 levels.Consistently,in vivo,wbp2 expression is upregulated following antagomir-133b treatment and downregulated following agomir-133b.RNA fluorescence in situ hybridization(RNA-FISH)results reveal miR-133b and wbp2 co-localization in ovarian interstitial cells.Notably,phylogenetic analysis indicates that miR-133b-3p and wbp2 are highly conserved among bony fish species.Additionally,dual-luciferase assays in other bony fish species including Oreochromis niloticus and Danio rerio also confirm the targeting effect of miR-133b-3p on wbp2,suggesting that this regulatory mechanism is conserved across bony fish.This research provides a theoretical foundation for further exploration of non-coding RNA-mediated regulation in gonadal development in teleost.展开更多
文摘目的:探究环氧化酶-2(cyclooxygenase-2,COX-2)、分泌型卷曲相关蛋白4(secreted frizzled-related protein 4,SFRP4)和WW域结合蛋白2(WW-domain binding protein 2,WBP2)在子宫腺肌病中的表达及其与子宫腺肌病临床特征的关系。方法:选取2019年1月-2020年6月在郑州大学第三附属医院确诊子宫腺肌病并进行手术切除子宫的患者40例,收集40例患者的异位内膜标本,统计患者的痛经、月经量及子宫大小等临床资料。采用免疫组化SP法分别检测COX-2、SFRP4和WBP2在子宫腺肌病异位病灶中的表达,分析其相互关系及其与子宫腺肌病临床特征的关系。结果:COX-2和SFRP4的表达呈正相关(r_(s)=0.533,P<0.01),COX-2和WBP2的表达呈正相关(r_(s)=0.544,P<0.01),WBP2和SFRP4的表达呈正相关(r_(s)=0.574,P<0.01)。无或轻度痛经及中度痛经组与重度痛经组COX-2和SFRP4低表达与高表达分布情况比较,差异均有统计学意义(P<0.05),而两组WBP2低表达与高表达分布情况比较,差异无统计学意义(P>0.05)。子宫体积正常组与子宫体积增大组COX-2、SFRP4及WBP2低表达与高表达分布情况比较,差异均无统计学意义(P>0.05)。月经量正常组与月经量增大组COX-2、SFRP4及WBP2低表达与高表达分布情况比较,差异均无统计学意义(P>0.05)。结论:COX-2、SFRP4和WBP2可能参与了子宫腺肌病的发生发展,COX-2可作为治疗子宫腺肌病潜在的分子靶点。
基金The Fund of Southern Marine Science and Engineering Guangdong Laboratory(Zhanjiang)under contract No.ZJW-2023-01the Guangdong Basic and Applied Basic Research Foundation under contract Nos 2023A1515010576 and 2024A1515012859the Marine Youth Talent Project of Zhanjiang under contract No.2023E0006.
文摘Sex-biased microRNAs(miRNAs)influence gonadal development in fish by directly targeting genes associated with estrogen production pathways.WW domain-binding protein 2(WBP2)functions as a crucial transcriptional co-activator of the estrogen and progesterone receptors(PGR).This study investigates the direct modulation of a sex-biased miR-133b on wbp2 and its regulatory role in gonadal development in fish,the greater amberjack(Seriola dumerili).Using dual-luciferase reporter assays,we demonstrate that wbp2 is a direct target of miR-133b,with miR-133b-3p binding to the 3'untranslated region(3'UTR)of wbp2.In vitro,miR-133b mimic significantly downregulate wbp2 expression,while the miR-133b inhibitor increase wbp2 levels.Consistently,in vivo,wbp2 expression is upregulated following antagomir-133b treatment and downregulated following agomir-133b.RNA fluorescence in situ hybridization(RNA-FISH)results reveal miR-133b and wbp2 co-localization in ovarian interstitial cells.Notably,phylogenetic analysis indicates that miR-133b-3p and wbp2 are highly conserved among bony fish species.Additionally,dual-luciferase assays in other bony fish species including Oreochromis niloticus and Danio rerio also confirm the targeting effect of miR-133b-3p on wbp2,suggesting that this regulatory mechanism is conserved across bony fish.This research provides a theoretical foundation for further exploration of non-coding RNA-mediated regulation in gonadal development in teleost.
