Bud dormancy represents a critical adaptation mechanism in perennial fruit plants,enabling survival during unfavorable environmental conditions.While this adaptive strategy is essential for plant survival and reproduc...Bud dormancy represents a critical adaptation mechanism in perennial fruit plants,enabling survival during unfavorable environmental conditions.While this adaptive strategy is essential for plant survival and reproduction,its molecular mechanisms remain incompletely understood.This study investigated two grape transcription factors:EARLY BUD BREAK(VvEBB)and SHORT VEGETATIVE PHASE 4(VvSVP4).Through heterologous transformation in poplar,the research demonstrated that VvSVP4 functions as a negative regulator of bud break,while VvEBB serves as a positive regulator.Transcriptome analysis revealed significant enrichment of plant hormone signaling pathways particularly ABA,IAA,and CK in plants overexpressing VvSVP4(VvSVP4-OE)and VvEBB(VvEBB-OE)compared to controls.Endogenous hormone level changes correlated positively with transcriptome data.During endodormancy,VvSVP4 directly and positively regulated the ABA receptor gene Vv PYL9 expression,maintaining bud dormancy.Conversely,during ecodormancy,rapid upregulation of VvEBB negatively regulated the expression of sucrose nonfermenting 1-related protein kinase 2 gene(Vv SAPK2),facilitating dormancy release.This study provides comprehensive insights into how VvSVP4 and VvEBB genes regulate dormancy and bud break through cell cycle regulation and multiple hormone signaling pathways.展开更多
基金supported by the China Agriculture Research System of MOF and MARA(CARS-29)the Xinjiang Uygur Autonomous Region Tianchi Talent Introduction Program,Chinathe Key R&D Technology Commissioner Projects in Hainan Province,China(ZDYF2024KJTPY008)。
文摘Bud dormancy represents a critical adaptation mechanism in perennial fruit plants,enabling survival during unfavorable environmental conditions.While this adaptive strategy is essential for plant survival and reproduction,its molecular mechanisms remain incompletely understood.This study investigated two grape transcription factors:EARLY BUD BREAK(VvEBB)and SHORT VEGETATIVE PHASE 4(VvSVP4).Through heterologous transformation in poplar,the research demonstrated that VvSVP4 functions as a negative regulator of bud break,while VvEBB serves as a positive regulator.Transcriptome analysis revealed significant enrichment of plant hormone signaling pathways particularly ABA,IAA,and CK in plants overexpressing VvSVP4(VvSVP4-OE)and VvEBB(VvEBB-OE)compared to controls.Endogenous hormone level changes correlated positively with transcriptome data.During endodormancy,VvSVP4 directly and positively regulated the ABA receptor gene Vv PYL9 expression,maintaining bud dormancy.Conversely,during ecodormancy,rapid upregulation of VvEBB negatively regulated the expression of sucrose nonfermenting 1-related protein kinase 2 gene(Vv SAPK2),facilitating dormancy release.This study provides comprehensive insights into how VvSVP4 and VvEBB genes regulate dormancy and bud break through cell cycle regulation and multiple hormone signaling pathways.
