Anthocyanins are important pigments and nutrients in fruits.Red grape is popular because of the high anthocyanin content.Previous studies have identified VvMYBA1 and its homologs as key regulators of fruit color;howev...Anthocyanins are important pigments and nutrients in fruits.Red grape is popular because of the high anthocyanin content.Previous studies have identified VvMYBA1 and its homologs as key regulators of fruit color;however,other transcription factors(TFs)that contribute to fruit color remain poorly understood.The present study identified the R2R3-MYB TF VvMYB24,whose gene expression levels were significantly higher in red berries(L51,Vitis vinifera×Vitis labrusca L.)than in green berries(L20,V.vinifera×V.labrusca L.).Overexpression of VvMYB24 in grape calli increased anthocyanin biosynthesis by upregulating the expression of specific structural genes(VvDFR and VvUFGT).Furthermore,VvMYB24 interacted with VvMYBA1 to form a protein complex that additionally increased the expression of VvDFR and VvUFGT.In addition,light-responsive TF VvHY5 could bind to the VvMYB24 promoters to activate its transcription.Taken together,the results reveal a regulatory module,VvHY5-VvMYB24-VvMYBA1,that influences anthocyanin biosynthesis in grape.展开更多
Excessive exposure to high light can lead to photoinhibition,which impairs photosynthetic efficiency and causes oxidative damage in plants,such as sunburn in grapevines.This study investigates the role of resveratrol(...Excessive exposure to high light can lead to photoinhibition,which impairs photosynthetic efficiency and causes oxidative damage in plants,such as sunburn in grapevines.This study investigates the role of resveratrol(Res),a stilbenoid with antioxidant properties,in protecting plants from high light damage.We found that exposure to high light increased reactive oxygen species(ROS)accumulation and induced photoinhibition in grapevine leaves.In response,Res biosynthesis was upregulated,along with an increase in stilbene synthase(VvSTS)expression.Application of exogenous Res alleviated ROS accumulation and improved photosynthetic efficiency.Further analysis revealed that the VvHY5-VvBEE1 regulatory module plays a pivotal role in regulating VvSTS expression under high light conditions.Specifically,VvHY5 activated VvSTS expression,while VvBEE1 repressed it.Transgenic analysis showed that overexpression of VvHY5 enhanced Res production and photoprotection,whereas overexpression of VvBEE1 reduced Res levels and exacerbated light-induced damage.VvHY5 and VvBEE1 competed for binding to the VvSTS promoter,with brassinosteroids(BRs)modulating their interaction.Our findings reveal the interplay between light signaling and brassinosteroid pathways in regulating Res biosynthesis,providing insights for protecting grapevines from sunburn.展开更多
基金supported by the National Natural Science Foundation of China(Grant No.31972368)the China Agriculture Research System(Grant No.CARS-29-yc-6)+1 种基金the Major Agricultural Science Projects of Liaoning Province(Grant No.2023JH1/10200004)the Science and Technology Program of Shenyang(Grant No.23-410-2-03).
文摘Anthocyanins are important pigments and nutrients in fruits.Red grape is popular because of the high anthocyanin content.Previous studies have identified VvMYBA1 and its homologs as key regulators of fruit color;however,other transcription factors(TFs)that contribute to fruit color remain poorly understood.The present study identified the R2R3-MYB TF VvMYB24,whose gene expression levels were significantly higher in red berries(L51,Vitis vinifera×Vitis labrusca L.)than in green berries(L20,V.vinifera×V.labrusca L.).Overexpression of VvMYB24 in grape calli increased anthocyanin biosynthesis by upregulating the expression of specific structural genes(VvDFR and VvUFGT).Furthermore,VvMYB24 interacted with VvMYBA1 to form a protein complex that additionally increased the expression of VvDFR and VvUFGT.In addition,light-responsive TF VvHY5 could bind to the VvMYB24 promoters to activate its transcription.Taken together,the results reveal a regulatory module,VvHY5-VvMYB24-VvMYBA1,that influences anthocyanin biosynthesis in grape.
基金National Natural Science Foundation of China(32472664)the Chinese Universities Scientific Fund(15053348,15054003)the 2115 Talent Development Program of China Agricultural University.
文摘Excessive exposure to high light can lead to photoinhibition,which impairs photosynthetic efficiency and causes oxidative damage in plants,such as sunburn in grapevines.This study investigates the role of resveratrol(Res),a stilbenoid with antioxidant properties,in protecting plants from high light damage.We found that exposure to high light increased reactive oxygen species(ROS)accumulation and induced photoinhibition in grapevine leaves.In response,Res biosynthesis was upregulated,along with an increase in stilbene synthase(VvSTS)expression.Application of exogenous Res alleviated ROS accumulation and improved photosynthetic efficiency.Further analysis revealed that the VvHY5-VvBEE1 regulatory module plays a pivotal role in regulating VvSTS expression under high light conditions.Specifically,VvHY5 activated VvSTS expression,while VvBEE1 repressed it.Transgenic analysis showed that overexpression of VvHY5 enhanced Res production and photoprotection,whereas overexpression of VvBEE1 reduced Res levels and exacerbated light-induced damage.VvHY5 and VvBEE1 competed for binding to the VvSTS promoter,with brassinosteroids(BRs)modulating their interaction.Our findings reveal the interplay between light signaling and brassinosteroid pathways in regulating Res biosynthesis,providing insights for protecting grapevines from sunburn.
