Fungi represent a valuable source of bioactive compounds,offering potential applications in functional foods and pharmaceuticals.Fistulina hepatica(Schaeff.)With.and Volvopluteus gloiocephalus(DC.)Vizzini,Contu&Ju...Fungi represent a valuable source of bioactive compounds,offering potential applications in functional foods and pharmaceuticals.Fistulina hepatica(Schaeff.)With.and Volvopluteus gloiocephalus(DC.)Vizzini,Contu&Justo,two autochthonous fungi from Serbia,remain underexplored for their mycochemical composition and biological activities.This study aims to evaluate their bioactive potential,emphasizing antioxidant,enzyme inhibition,and cytotoxic properties.Extracts from F.hepatica and V.gloiocephalus were obtained using solvents of different polarities(acetone,CHCl3,80%MeOH,70%EtOH,H_(2)O and PSH),and dried fruiting bodies were subjected to chemical analysis.The chemical analyses included Fourier–transform infrared spectroscopy(FTIR)analysis with mycroanalysis,liquid chromatography–mass spectrometry(LC-MS/MS),and atomic absorption spectroscopy(AAS)profiling.While AAS profiling of minerals was conducted on the dried fruiting bodies,all other analyses were performed on the extracts.In vitro assays were performed to assess the antioxidant capacity,cytotoxicity against CCRF-CEM cells,and digestive enzyme inhibition(α-amylase,α-glucosidase,and lipase)of the extracts.Both fungi exhibited significant antioxidant activity comparable to the standard antioxidant compound propyl gallate.Additionally,extracts showed potent enzyme inhibition,including hypoglycemic and lipase activities,indicating their potential as hypoglycemic and hypolipidemic agents,particularly in F.hepatica.Conversely,the acetone extract of V.gloiocephalus demonstrated notable cytotoxicity against CCRF-CEM cancer cells.PCA analysis confirmed correlations between bioactive compounds and bioactivities.These findings highlight the potential of F.hepatica and V.gloiocephalus as promising sources of natural bioactive compounds with applications in addressing metabolic disorders,cancer and oxidative stress,as functional foods,nutraceuticals,and pharmaceuticals.展开更多
Volvopluteus gloiocephalus is an underexplored edible mushroom with potential anti-inflammatory and metabolic regulatory properties.This study aimed to evaluate its effects on inflammatory mediators and steroid-relate...Volvopluteus gloiocephalus is an underexplored edible mushroom with potential anti-inflammatory and metabolic regulatory properties.This study aimed to evaluate its effects on inflammatory mediators and steroid-related molecular targets using a bioactivity-guided approach.To capture metabolites of different polarities,five sol-vent extracts(acetone,CHCl_(3),80%MeOH,70%EtOH,and H_(2)O)were prepared,while polysaccharide-rich fraction(PSH)was prepared to investigate the contribution of high-molecular-weight constituents,particu-larlyβ-glucans.Anti-inflammatory activity was assessed via inhibition of arachidonic acid metabolites in human platelets and direct COX-1/COX-2 enzyme assays.Steroid receptor binding(ERα,ERβ,AR,and GR)was evalu-ated using a yeast-based fluorescent biosensor,while AKR1C3 and AKR1C4 inhibition was determined by a NADPH-consumption assay.Structural characterization of the PSH fraction was performed by ^(1)H NMR,and molecular docking explored interactions of quantified polyphenolics with AKR1C3 and COX enzymes.The extracts exhibited pronounced,pathway-dependent anti-inflammatory activity.CHCl_(3)showed the strongest inhibition of the 12-LOX-derived metabolite 12-HETE(89.45%),while the 80%MeOH markedly reduced COX-derived metabolites PGE2 and TXB2(84.50%and 82%,respectively).Notably,PSH fraction and polar extracts demonstrated strong and selective glucocorticoid receptor binding without estrogenic or andro-genic activity.All extracts except 80%MeOH potently inhibited AKR1C3,with the PSH fraction showing the strongest effect(96.17%)exceeding ibuprofen.^(1)H NMR indicated enrichment inβ-glucans.Although docking supported phenolic-enzyme interactions,experimental findings suggest a substantial contribution of poly-saccharides and other non-phenolic constituents.These results highlight V.gloiocephalus as a promising multitarget bioactive source for functional food and nutraceutical applications.展开更多
基金supported by the Ministry of Science,Technological Development,and Innovation of the Republic of Serbia(Grant No.451-03-66/2024-03/200125&451-03-65/2024-03/200125).
文摘Fungi represent a valuable source of bioactive compounds,offering potential applications in functional foods and pharmaceuticals.Fistulina hepatica(Schaeff.)With.and Volvopluteus gloiocephalus(DC.)Vizzini,Contu&Justo,two autochthonous fungi from Serbia,remain underexplored for their mycochemical composition and biological activities.This study aims to evaluate their bioactive potential,emphasizing antioxidant,enzyme inhibition,and cytotoxic properties.Extracts from F.hepatica and V.gloiocephalus were obtained using solvents of different polarities(acetone,CHCl3,80%MeOH,70%EtOH,H_(2)O and PSH),and dried fruiting bodies were subjected to chemical analysis.The chemical analyses included Fourier–transform infrared spectroscopy(FTIR)analysis with mycroanalysis,liquid chromatography–mass spectrometry(LC-MS/MS),and atomic absorption spectroscopy(AAS)profiling.While AAS profiling of minerals was conducted on the dried fruiting bodies,all other analyses were performed on the extracts.In vitro assays were performed to assess the antioxidant capacity,cytotoxicity against CCRF-CEM cells,and digestive enzyme inhibition(α-amylase,α-glucosidase,and lipase)of the extracts.Both fungi exhibited significant antioxidant activity comparable to the standard antioxidant compound propyl gallate.Additionally,extracts showed potent enzyme inhibition,including hypoglycemic and lipase activities,indicating their potential as hypoglycemic and hypolipidemic agents,particularly in F.hepatica.Conversely,the acetone extract of V.gloiocephalus demonstrated notable cytotoxicity against CCRF-CEM cancer cells.PCA analysis confirmed correlations between bioactive compounds and bioactivities.These findings highlight the potential of F.hepatica and V.gloiocephalus as promising sources of natural bioactive compounds with applications in addressing metabolic disorders,cancer and oxidative stress,as functional foods,nutraceuticals,and pharmaceuticals.
基金supported by the Ministry of Science,Technological Development and Innovation of the Republic of Serbia(Grant Nos.451-03-33/2026-03/200125 and 451-03-34/2026-03/200125).
文摘Volvopluteus gloiocephalus is an underexplored edible mushroom with potential anti-inflammatory and metabolic regulatory properties.This study aimed to evaluate its effects on inflammatory mediators and steroid-related molecular targets using a bioactivity-guided approach.To capture metabolites of different polarities,five sol-vent extracts(acetone,CHCl_(3),80%MeOH,70%EtOH,and H_(2)O)were prepared,while polysaccharide-rich fraction(PSH)was prepared to investigate the contribution of high-molecular-weight constituents,particu-larlyβ-glucans.Anti-inflammatory activity was assessed via inhibition of arachidonic acid metabolites in human platelets and direct COX-1/COX-2 enzyme assays.Steroid receptor binding(ERα,ERβ,AR,and GR)was evalu-ated using a yeast-based fluorescent biosensor,while AKR1C3 and AKR1C4 inhibition was determined by a NADPH-consumption assay.Structural characterization of the PSH fraction was performed by ^(1)H NMR,and molecular docking explored interactions of quantified polyphenolics with AKR1C3 and COX enzymes.The extracts exhibited pronounced,pathway-dependent anti-inflammatory activity.CHCl_(3)showed the strongest inhibition of the 12-LOX-derived metabolite 12-HETE(89.45%),while the 80%MeOH markedly reduced COX-derived metabolites PGE2 and TXB2(84.50%and 82%,respectively).Notably,PSH fraction and polar extracts demonstrated strong and selective glucocorticoid receptor binding without estrogenic or andro-genic activity.All extracts except 80%MeOH potently inhibited AKR1C3,with the PSH fraction showing the strongest effect(96.17%)exceeding ibuprofen.^(1)H NMR indicated enrichment inβ-glucans.Although docking supported phenolic-enzyme interactions,experimental findings suggest a substantial contribution of poly-saccharides and other non-phenolic constituents.These results highlight V.gloiocephalus as a promising multitarget bioactive source for functional food and nutraceutical applications.
