Leontopodium alpinum is a traditional alpine medicinal plant,is extensively used in Tibetan and Mongolian medicine.Its applicability in managing febrile epidemics and respiratory conditions requires deeper study,espec...Leontopodium alpinum is a traditional alpine medicinal plant,is extensively used in Tibetan and Mongolian medicine.Its applicability in managing febrile epidemics and respiratory conditions requires deeper study,especially given the recurring threat of infectious diseases.This study uses bibliometric analysis to examine the current state and trends in Leontopodium alpinum research,integrates scattered information,and suggests pathways for subsequent investigation.This study retrieved Chinese literature from the China National Knowledge Infrastructure(CNKI)platform and international literature from the Web of Science(WoS),with data collection finalized by March 2025.CiteSpace 6.3.R1 and VOSviewer 1.6.20 were used to visually analyze publication trends,keyword co-occurrence,and author collaboration networks.The analysis has found that Chinese publications rose markedly after 2003 but declined after 2019,focusing largely on chemical components–such as chlorogenic acid and volatile oils–and pharmacological activities.International output,though smaller,grew steadily,with an emphasis on compositional analysis and antioxidant effects.Keyword clustering revealed that both Chinese and global research expanded from foundational taxonomy to chemical constituents,bioactivity,and environmental adaptation.Core author and institutional analyses identified Liaoning University of Traditional Chinese Medicine and the University of Chinese Academy of Sciences as domestic leaders,while the University of Innsbruck(Austria)played a leading international role.In conclusion,this study offers the first systematic summary of research and publishing trends for Leontopodium alpinum,while also identifying key knowledge gaps.Future studies should prioritize multi-omics approaches to clarify the mechanisms of its bioactive compounds and advance clinical application.Due to its notable efficacy against febrile epidemics and respiratory diseases,expanded research is strongly recommended–particularly in light of rising infectious disease risks where this species could serve an essential role.展开更多
Temporary plugging and diversion fracturing(TPDF)is widely used to promote the uniform and complex distribution of multi-clustered hydraulic fractures(HFs)in a horizontal well of the unconventional formations.However,...Temporary plugging and diversion fracturing(TPDF)is widely used to promote the uniform and complex distribution of multi-clustered hydraulic fractures(HFs)in a horizontal well of the unconventional formations.However,the migration behavior of temporary plugging agent(TPA),as a function of the concentration and particle size of TPA and cluster-perforation numbers,etc.,determining the effectiveness of this technique,remains unclear.Therefore,this study conducted innovatively a series of TPDF simulation experiments on transparent polymethyl methacrylate(PMMA)specimens(cubic block of 30 cm×30 cm×30 cm)to explore visually the migration behavior of TPA in multi-clustered HFs in a horizontal well.A laboratory hydraulic sandblasting perforation completion technique was implemented to simulate the multi-cluster perforations.All the distributions of wellbore,perforations,HFs,and TPA can be seen clearly inside the PMMA specimen post the experiment.The results show that there are four characteristic plugging positions for the TPA:mouth of HF,middle of HF,tip of HF,and the intersection of HFs.Small particle size TPA tends to migrate to the fracture tip for plugging,while large particle size TPA tends to plug at the fracture mouth.The migration of the TPA is influenced obviously by the morphology of the fracture wall.A smooth fracture wall is conducive to the migration of the TPA to the far end of HFs,but not conducive to generating the plugging zone and HF diversion.In contrast,a"leaf vein"fracture of rough wall is conducive to generating the plugging layer and the diversion of HFs,but not conducive to the migration of the TPA to the far end of HFs.The migration ability of TPA in a"shell"pattern is intermediate between the two above cases.Increasing TPA concentration can encourage TPA to migrate more quickly to the characteristic plugging position,and thereby to promote the creation of effective plugging and subsequently the multi-stage diversion of the HFs.Nevertheless,excessive concentration may cause the TPA to settle prematurely,affecting the propagation of the HFs to the far end.Increasing the number of clusters to a certain extent can encourage TPA to migrate into the HFs and form plugging,and promote the diversion.An evaluation system for the migration ability of granular TPA has been established,and it was calculated that when there is no plugging expectation target,the comprehensive migration ability of small particle size TPA is stronger than that of large particle size TPA.This research provides theoretical foundation for the optimization of temporary plugging parameters.展开更多
Cerebral or intracranial aneurysm is a leading cause of subarachnoid hemorrhage. It was initially treated with open surgical clipping but as rapid development of technology a less invasive endovascular coiling techniq...Cerebral or intracranial aneurysm is a leading cause of subarachnoid hemorrhage. It was initially treated with open surgical clipping but as rapid development of technology a less invasive endovascular coiling technique of aneurysm revolutionized the treatment. Due to tortuous anatomy of parent artery and complicated morphology and location of aneurysm there is ongoing challenge in the complete obliteration of aneurysms. To aid in the advances of treating aneurysm stent assisted endovascular coiling was introduced to give more scaffold support to parent artery and decrease events of coil protrusion from aneurysms. Many types and generation of stents were developed. One of the most recently introduced stent is low profile visualized intraluminal support (LVIS) stent. Due to its low profile nature it can be used in 0.017-inch inner diameter microcatheter and reach small and complex vessels providing high aneurysmal neck coverage which was not possible through other traditional stent. In addition, its braided design with tantalum strands and radiopaque markers make it more visible during stent placement and post procedure stent evaluation. Despite of many advantages of LVIS stent it is related to high rate of thromboembolic complications and technical complications. Aim of this review paper was to evaluate therapeutic safety, effectiveness and feasibility of LVIS stent in endovascular coil embolization of intracranial aneurysms.展开更多
The development of uranyl ion detection technology has exhibited its significance in public security and environmental fields for the radioactivity and chemical toxicity of uranyl ion.The WHO standard of uranyl ion ma...The development of uranyl ion detection technology has exhibited its significance in public security and environmental fields for the radioactivity and chemical toxicity of uranyl ion.The WHO standard of uranyl ion makes it necessary to develop highly sensitive uranyl rapid warning system in drinking water monitoring.Herein,a visualized rapid warning system for trace uranyl ion is carried out based on electrochemiluminescence(ECL) imaging technology to give an ultra-low limit of detection(LOD) and high selectivity.Amidoxime,a bi-functional group with both uranyl ion capturing and co-reactive functions,is modified on a conjugated polymer backbone with strong ECL signal to be prepared into three-in-one polymer nanoparticles(PNPs) with self-enhanced ECL property.The captured uranyl ion can enhance the ECL signal of PNPs via resonance energy transfer process to give the LOD as 0.5 ng/L,which is much lower than the known luminescent uranyl sensors.Furthermore,ECL imaging technology is introduced into realizing visualized uranyl rapid warning,and can be successfully applied on natural water samples.This study provides a novel strategy for uranyl rapid warning,and shows its potential meaning in public security and environmental fields.展开更多
This study presents a robustness optimization method for rapid prototyping(RP)of functional artifacts based on visualized computing digital twins(VCDT).A generalized multiobjective robustness optimization model for RP...This study presents a robustness optimization method for rapid prototyping(RP)of functional artifacts based on visualized computing digital twins(VCDT).A generalized multiobjective robustness optimization model for RP of scheme design prototype was first built,where thermal,structural,and multidisciplinary knowledge could be integrated for visualization.To implement visualized computing,the membership function of fuzzy decision-making was optimized using a genetic algorithm.Transient thermodynamic,structural statics,and flow field analyses were conducted,especially for glass fiber composite materials,which have the characteristics of high strength,corrosion resistance,temperature resistance,dimensional stability,and electrical insulation.An electrothermal experiment was performed by measuring the temperature and changes in temperature during RP.Infrared thermographs were obtained using thermal field measurements to determine the temperature distribution.A numerical analysis of a lightweight ribbed ergonomic artifact is presented to illustrate the VCDT.Moreover,manufacturability was verified based on a thermal-solid coupled finite element analysis.The physical experiment and practice proved that the proposed VCDT provided a robust design paradigm for a layered RP between the steady balance of electrothermal regulation and manufacturing efficacy under hybrid uncertainties.展开更多
In a Mach 3.8 wind tunnel, both instantaneous and time-averaged flow structures of different scales around a blunt double-cone with or without supersonic film cooling were visualized via nano-tracer planar laser scatt...In a Mach 3.8 wind tunnel, both instantaneous and time-averaged flow structures of different scales around a blunt double-cone with or without supersonic film cooling were visualized via nano-tracer planar laser scattering （NPLS）, which has a high spatiotemporal resolution. Three experimental cases with different injection mass flux rates were carried out. Many typical flow structures were clearly shown, such as shock waves, expansion fans, shear layers, mixing layers, and turbulent boundary layers. The analysis of two NPLS images with an interval of 5 us revealed the temporal evolution characteristics of flow structures. With matched pressures, the laminar length of the mixing layer was longer than that in the case with a larger mass flux rate, but the full covered region was shorter. Structures like K-H （Kelvin-Helmholtz） vortices were clearly seen in both flows. Without injection, the flow was similar to the supersonic flow over a backward- facing step, and the structures were relatively simpler, and there was a longer laminar region. Large scale structures such as hairpin vortices were visualized. In addition, the results were compared in part with the schlieren images captured by others under similar conditions.展开更多
To work efficiently with DSS, most users need assistance in representation conversion, i. e., translating the specific outcome from the DSS into the universal language of visual. In generally, it is much easier to und...To work efficiently with DSS, most users need assistance in representation conversion, i. e., translating the specific outcome from the DSS into the universal language of visual. In generally, it is much easier to understand the results from the DSS if they are translated into charts, maps, and other scientific displays, because visualization exploits human natural ability to recognize and understand visual pattern. In this paper we discuss the concept of visualization for DSS. AniGraftool, a software system, is introduced as an example of Visualized User Interface for DSS.展开更多
A micro-nano pore three-dimensional visualized real-time physical simulation of natural gas charging, in-situ pore-scale computation, pore network modelling, and apparent permeability evaluation theory were used to in...A micro-nano pore three-dimensional visualized real-time physical simulation of natural gas charging, in-situ pore-scale computation, pore network modelling, and apparent permeability evaluation theory were used to investigate laws of gas and water flow and their distribution, and controlling factors during the gas charging process in low-permeability(tight) sandstone reservoir. By describing features of gas-water flow and distribution and their variations in the micro-nano pore system, it is found that the gas charging in the low permeability(tight) sandstone can be divided into two stages, expansion stage and stable stage. In the expansion stage, the gas flows continuously first into large-sized pores then small-sized pores, and first into centers of the pores then edges of pores;pore-throats greater than 20 μm in radius make up the major pathway for gas charging. With the increase of charging pressure, movable water in the edges of large-sized pores and in the centers of small pores is displaced out successively. Pore-throats of 20-50 μm in radius and pore-throats less than 20 μm in radius dominate the expansion of gas charging channels at different stages of charging in turn, leading to reductions in pore-throat radius, throat length and coordination number of the pathway, which is the main increase stage of gas permeability and gas saturation. Among which, pore-throats 30-50 μm in radius control the increase pattern of gas saturation. In the stable stage, gas charging pathways have expanded to the maximum, so the pathways keep stable in pore-throat radius, throat length, and coordination number, and irreducible water remains in the pore system, the gas phase is in concentrated clusters, while the water phase is in the form of dispersed thin film, and the gas saturation and gas permeability tend stable. Connected pore-throats less than 20 μm in radius control the expansion limit of the charging pathways, the formation of stable gas-water distribution, and the maximum gas saturation. The heterogeneity of connected pore-throats affects the dynamic variations of gas phase charging and gas-water distribution. It can be concluded that the pore-throat configuration and heterogeneity of the micro-nanometer pore system control the dynamic variations of the low-permeability(tight) sandstone gas charging process and gas-water distribution features.展开更多
Purified terephthalic acid(PTA) is an important chemical raw material. P-xylene(PX) is transformed to terephthalic acid(TA) through oxidation process and TA is refined to produce PTA. The PX oxidation reaction is a co...Purified terephthalic acid(PTA) is an important chemical raw material. P-xylene(PX) is transformed to terephthalic acid(TA) through oxidation process and TA is refined to produce PTA. The PX oxidation reaction is a complex process involving three-phase reaction of gas, liquid and solid. To monitor the process and to improve the product quality, as well as to visualize the fault type clearly, a fault diagnosis method based on selforganizing map(SOM) and high dimensional feature extraction method, local tangent space alignment(LTSA),is proposed. In this method, LTSA can reduce the dimension and keep the topology information simultaneously,and SOM distinguishes various states on the output map. Monitoring results of PX oxidation reaction process indicate that the LTSA–SOM can well detect and visualize the fault type.展开更多
Knowledge of migration and retention mechanisms of elastic gel particles(EGPs)in pore-throats is essential for the effective application of EGPs as a smart sweep improvement and profile control agent for enhanced oil ...Knowledge of migration and retention mechanisms of elastic gel particles(EGPs)in pore-throats is essential for the effective application of EGPs as a smart sweep improvement and profile control agent for enhanced oil recovery(EOR).The matching coefficient(defined as the ratio of particle size to pore-throat size)is used to investigate its influence on migration,retention and profile control performance of EGPs.A 1-D continuous pore-throat visualization model(PTVM),a 2-D heterogeneous PTVM and a 3-D heterogeneous core model were constructed and used to investigate pore-scale migration,retention and controlling mechanism of migration and retention characteristics on EGPs profile control.The results of the 1-D continuous PTVM indicated that while the matching coefficient was in the optimal range(i.e.,0.20-0.32),the EGPs could not only smoothly migrate to the deeper pore-throats,but also form stable retention in the pores to resist the erosion of injected water,which was conducive to the effective indepth profile control.The results of the 2-D heterogeneous PTVM verified that the sweep efficiency in low-permeability regions could be significantly improved by in-depth migration and stable retention of EGPs in the pore-throats with an optimal matching coefficient(0.29),which was much better than that in cases with a smaller matching coefficient(0.17)or an excessive matching coefficient(0.39).Moreover,the NMR displacement experiments of 3-D heterogeneous cores were carried out to simulate the EGPs profile control in actual reservoir porous media.Saturation images and T2 spectrum curves of crude oil showed that EOR in the low-permeability layer was highest(56.1%)using EGPs profile control with an optimal matching coefficient,attributing to the in-depth migration and stable retention of EGPs.展开更多
With the social development, we are stepping into an information technology world. In such a world, our life is getting more and more diversified and rich because of e-business. E-business not only provides us conveni...With the social development, we are stepping into an information technology world. In such a world, our life is getting more and more diversified and rich because of e-business. E-business not only provides us convenience but also large amounts of business data. However, how shall we better store, manage and use these business data has become a major field being studied by e-business. With the rapid growth of data volume, the relational database system cannot meet the requirements of the current status. In this paper, focusing on the visualized analysis model of Hadoop business data, it analyzed the business data in terms of the visualized platform, database and analysis model etc. Depending on the analysis, offline-data analysis and data visualization for Hive database will be greatly improved, so that references and suggestions can be provided for the visualized analysis model of Hadoop business data.展开更多
Objective: This study visualizes and analyzes the current status, hotspots, and frontiers of cardiac rehabilitation for patients with Coronary Heart Disease (CHD) over the past decade (2012.01-2022.12), and explores t...Objective: This study visualizes and analyzes the current status, hotspots, and frontiers of cardiac rehabilitation for patients with Coronary Heart Disease (CHD) over the past decade (2012.01-2022.12), and explores the future development trend and research direction of the CR for CHD patients in China. Methods: Relevant literature was searched, screened and downloaded from the Web of Science (WOS) database, and bibliometric and visualization analyses were performed using CiteSpace VI software. Results: Through the search and screening of related literature, 2443 English articles were finally included. Among them, most of the Chinese publishers were mainly universities and had less connection with each other, while the foreign publishers were mainly universities and medical institutions in the United States and Europe, and had close connection with each other. The research content of Chinese scholars mainly focuses on the assessment of patients’ life and psychological status, as well as the assessment of cardiac function. Foreign research focuses on physical training assessment, disease perception, etc. Conclusion: Through visualizing relevant research with CiteSpace VI software in the form of a knowledge map, the research frontiers and trends in the field of cardiac rehabilitation for coronary heart disease patients in China and abroad can be discovered more intuitively. Compared with foreign research, the development of cardiac rehabilitation for coronary heart disease patients in China is relatively slow and insufficient, and institutions lack cooperation. In the future, China should accelerate the connection between regions in the field of cardiac rehabilitation and develop a cardiac rehabilitation model suitable for coronary heart disease patients in China with its own characteristics.展开更多
The 2020 TKK Science Award in I Life Sciences went to CAS member SHI Yigong,a distinguished structural biologist,for his achievements in:elucidating the yeast and human splicing cycle in atomic detail,which allows us ...The 2020 TKK Science Award in I Life Sciences went to CAS member SHI Yigong,a distinguished structural biologist,for his achievements in:elucidating the yeast and human splicing cycle in atomic detail,which allows us to visually appreciate the dynamic process of the supermolecular machinery of spliceosome performing molecular surgery on the chain of a pre-message RNA(pre-mRNA)to give rise to a mature mRNA.展开更多
This study conducted a bibliometric and visualization analysis of 265 publications related to Gelsemium elegans Benth.(G.elegans)poisoning in China,retrieved from the China National Knowledge Infrastructure database c...This study conducted a bibliometric and visualization analysis of 265 publications related to Gelsemium elegans Benth.(G.elegans)poisoning in China,retrieved from the China National Knowledge Infrastructure database covering the years 1995–2025.It systematically summarizes the current status,developmental trends,and research hotspots in this field.The results indicate that research on G.elegans poisoning in China has evolved through three distinct phases:An initial stage from 1995 to 2005,rapid growth from 2005 to 2015,and a peak period after 2016,with publication peaks in 2019 and 2022,reflecting growing scientific and public attention to this issue.Key research institutions are primarily concentrated at the Hunan Agricultural University and Fujian University of Traditional Chinese Medicine,representing the core centers in the field.Keyword co-occurrence and clustering analyses revealed“G.elegans,”“poisoning,”and“Gelsemium alkaloids”as central themes,with studies on the toxicological mechanisms of indole alkaloids being particularly prominent.Research directions span toxicological mechanisms,epidemiology,and clinical management.Funding analysis further showed that the National Natural Science Foundation of China has provided substantial support for this field.Overall,research on G.elegans poisoning has advanced considerably,especially regarding alkaloid constituents and their toxic mechanisms.However,translational research,early diagnosis,antidote development,and misuse prevention remain underexplored.Future studies should emphasize multidisciplinary collaboration to promote advances in clinical management,toxicological assessment,and public health prevention strategies related to G.elegans poisoning.展开更多
In situ imaging diagnosis and precise treatment of deep tumor tissues are hotspots in life sciences and medical research.In recent years,using focused ultrasound to remotely control engineered bacteria for drug releas...In situ imaging diagnosis and precise treatment of deep tumor tissues are hotspots in life sciences and medical research.In recent years,using focused ultrasound to remotely control engineered bacteria for drug release has become one of the methods for precise in vivo drug delivery.However,non-visualized engineered bacteria pose challenges for precise control within the body.Therefore,there is an urgent need for an engineered bacterial vector capable of deep tissue imaging to precisely locate bacteria in vivo.Acoustic reporter genes(ARGs)are biological elements used for deep tissue imaging,with gene clusters over 8 kb.However,ARGs are often tested on plasmids,which hinders stable expression in vivo and limits the space for inserting components that regulate drug release.Therefore,we used the attenuated Salmonella typhimurium VNP20009,known for its tumor-targeting ability,as the chassis bacteria.By using CRISPR-Cas9 technology,we inserted ARGs into the genome and optimized the promoter strength and copy number for ARG expression,constructing ultrasound-visible engi-neered bacteria expressing gas vesicles on the genome.Additionally,by knocking out the stress protein gene htrA in VNP20009,we increased the maximum injection dose by tenfold and the tumor specificity by a hundredfold.The constructed ultrasound-visible engineered bacteria can stably synthesize gas vesicles and output ultrasound signals while directly carrying drug plasmids for tumor therapy.Our research provides an effective vector for diagnosis and precise treatment.展开更多
The development of intelligent textiles that integrate impact protection with real-time sensing capabilities is of critical importance for next-generation wearable protective systems.Despite extensive usage of convent...The development of intelligent textiles that integrate impact protection with real-time sensing capabilities is of critical importance for next-generation wearable protective systems.Despite extensive usage of conventional protective films/elastomers,their inherent planar geometries compromise wearing comfort,and the universal absence of real-time impact detection/location capabilities restricts application prospects.To address these challenges,an intelligent shear-stiffening-based mechanoluminescent fiber(ML-TPS)is developed through integrated wet-spinning and coating technology.This fiber combines a shear-stiffening polymer core with a ZnS:Cu/polydimethylsiloxane(PDMS)mechanoluminescent coating,synergistically enabling excellent impact resistance and spatiotemporal force visualization.The resultant 4 mm-thick ML-TPS fabric maintains exceptional flexibility,breathability,and high impact energy dissipation(efficiency>90%)while demonstrating rapid damage localization(response time<6 ms)and quantitative impact assessment(R2=0.95 linear correlation),surpassing conventional materials in temporal resolution.The fabricated visual sensing matrix enables visual localization,showing unique advantages in scenarios requiring rapid impact response,such as sports protection and personal safety.Finally,the multi-scenario applicability of ML-TPS fibers is demonstrated through human motion monitoring and underwater warning validation.This work provides a new paradigm for developing active protection-type intelligent wearable systems.展开更多
Biological visions have inspired the development of artificial vision systems with diverse visual functional traits,however,the detected wavelength is only in visible light between 0.4 and 0.78μm,restricting their ap...Biological visions have inspired the development of artificial vision systems with diverse visual functional traits,however,the detected wavelength is only in visible light between 0.4 and 0.78μm,restricting their applications.Snakes generate a thermal image of animals due to pit organs for detecting and converting infrared,allowing them to accurately target predators or prey even under darkness.Inspired by natural infrared visualized snakes,we propose artificial vision systems with CMOS sensors-integrated upconverters to break visible light limitations to realize 3840×2160 ultra-high-resolution short-wave infrared(SWIR)and mid-wave infrared(MWIR)visualization imaging for the first time.Through colloidal quantum dot barrier heterojunction architecture design of infrared detecting units and the introduction of co-hosted emitting units,the luminance and upconversion efficiency reach up to 6388.09 cd m^(−2) and 6.41%for SWIR,1311.64 cd m^(−2) and 4.06%for MWIR at room temperature.Our artificial vision systems broaden a wide spectrum of applications within infrared,such as night vision,agricultural science,and industry inspection,marking a significant advance in bioartificial vision.展开更多
In comparing with traditional polymer degradation toward unknown or unvalued products(i.e.,downcycling),new methods to degrade polymers into monomers or high-value-added materials(i.e.,upcycling)are preferable for sus...In comparing with traditional polymer degradation toward unknown or unvalued products(i.e.,downcycling),new methods to degrade polymers into monomers or high-value-added materials(i.e.,upcycling)are preferable for sustainable developments.In this work,CO_(2)-based linear and cross-linked unsaturated polyesters,that is,poly(alkynoate)s,were successfully degraded by benzamidine into diols and high-value-added pyrimidone or imidazolone derivatives through controlled reaction conditions.The degradation process could be visualized under UV light via gradually increased luminescent intensity of the reaction mixtures and fluorescence patterns could be generated through microcontact printing of the stamp on the thin films of poly(alkynoate)s.Moreover,the pyrimidone derivatives could be applied in bioimaging and inhibiting bacteria,and the imidazolone derivatives could be used for the detection and recovery of gold(III)ions from electronic waste and serve as the fluorescent sensor to detect in situ-generated amines from food spoilage.Thus,this work presents a visualized and high value productselective degradation to solve the end-of-life issue of polymers.展开更多
Small non-coding RNAs(sRNAs)have received much attention in recent years due to their unique biological properties,which can efficiently and specifically tune target gene expressions in bacteria.Inspired by natural sR...Small non-coding RNAs(sRNAs)have received much attention in recent years due to their unique biological properties,which can efficiently and specifically tune target gene expressions in bacteria.Inspired by natural sRNAs,recent works have proposed the use of artificial sRNAs(asRNAs)as genetic tools to regulate desired gene that has been applied in several fields,such as metabolic engineering and bacterial physiology studies.However,the rational design of asRNAs is still a challenge.In this study,we proposed structure and length as two criteria to implement rational visualized and precise design of asRNAs.T7 expression system was one of the most useful recombinant protein expression systems.However,it was deeply limited by the formation of inclusion body.To settle this problem,we designed a series of asRNAs to inhibit the T7 RNA polymerase(Gene1)expression to balance the rate between transcription and folding of recombinant protein.Based on the heterologous expression of Aspergillus oryzae Li-3 glucuronidase in E.coli,the asRNA-antigene1-17bp can effectively decrease the inclusion body and increase the enzyme activity by 169.9%.展开更多
基金supported by the National Key R&D Program of China:Intergovernmental Cooperation in International Science and Technology Innovation(2022YFE0119300)Mongolian Foundation for Science and Technology(MFST-CHN2022/277 and MFST 2022/176)Science and Technology Foundation,Ministry of Environment and Tourism of Mongolia(20BB11ShT3030).
文摘Leontopodium alpinum is a traditional alpine medicinal plant,is extensively used in Tibetan and Mongolian medicine.Its applicability in managing febrile epidemics and respiratory conditions requires deeper study,especially given the recurring threat of infectious diseases.This study uses bibliometric analysis to examine the current state and trends in Leontopodium alpinum research,integrates scattered information,and suggests pathways for subsequent investigation.This study retrieved Chinese literature from the China National Knowledge Infrastructure(CNKI)platform and international literature from the Web of Science(WoS),with data collection finalized by March 2025.CiteSpace 6.3.R1 and VOSviewer 1.6.20 were used to visually analyze publication trends,keyword co-occurrence,and author collaboration networks.The analysis has found that Chinese publications rose markedly after 2003 but declined after 2019,focusing largely on chemical components–such as chlorogenic acid and volatile oils–and pharmacological activities.International output,though smaller,grew steadily,with an emphasis on compositional analysis and antioxidant effects.Keyword clustering revealed that both Chinese and global research expanded from foundational taxonomy to chemical constituents,bioactivity,and environmental adaptation.Core author and institutional analyses identified Liaoning University of Traditional Chinese Medicine and the University of Chinese Academy of Sciences as domestic leaders,while the University of Innsbruck(Austria)played a leading international role.In conclusion,this study offers the first systematic summary of research and publishing trends for Leontopodium alpinum,while also identifying key knowledge gaps.Future studies should prioritize multi-omics approaches to clarify the mechanisms of its bioactive compounds and advance clinical application.Due to its notable efficacy against febrile epidemics and respiratory diseases,expanded research is strongly recommended–particularly in light of rising infectious disease risks where this species could serve an essential role.
基金supported by the National Natural Science Foundation of China Joint Fund for Enterprise Innovation and Development,Enrichment Mechanism and Stereoscopic Development of Shale Oil in Continental Rift Basins(No.U24B6002).
文摘Temporary plugging and diversion fracturing(TPDF)is widely used to promote the uniform and complex distribution of multi-clustered hydraulic fractures(HFs)in a horizontal well of the unconventional formations.However,the migration behavior of temporary plugging agent(TPA),as a function of the concentration and particle size of TPA and cluster-perforation numbers,etc.,determining the effectiveness of this technique,remains unclear.Therefore,this study conducted innovatively a series of TPDF simulation experiments on transparent polymethyl methacrylate(PMMA)specimens(cubic block of 30 cm×30 cm×30 cm)to explore visually the migration behavior of TPA in multi-clustered HFs in a horizontal well.A laboratory hydraulic sandblasting perforation completion technique was implemented to simulate the multi-cluster perforations.All the distributions of wellbore,perforations,HFs,and TPA can be seen clearly inside the PMMA specimen post the experiment.The results show that there are four characteristic plugging positions for the TPA:mouth of HF,middle of HF,tip of HF,and the intersection of HFs.Small particle size TPA tends to migrate to the fracture tip for plugging,while large particle size TPA tends to plug at the fracture mouth.The migration of the TPA is influenced obviously by the morphology of the fracture wall.A smooth fracture wall is conducive to the migration of the TPA to the far end of HFs,but not conducive to generating the plugging zone and HF diversion.In contrast,a"leaf vein"fracture of rough wall is conducive to generating the plugging layer and the diversion of HFs,but not conducive to the migration of the TPA to the far end of HFs.The migration ability of TPA in a"shell"pattern is intermediate between the two above cases.Increasing TPA concentration can encourage TPA to migrate more quickly to the characteristic plugging position,and thereby to promote the creation of effective plugging and subsequently the multi-stage diversion of the HFs.Nevertheless,excessive concentration may cause the TPA to settle prematurely,affecting the propagation of the HFs to the far end.Increasing the number of clusters to a certain extent can encourage TPA to migrate into the HFs and form plugging,and promote the diversion.An evaluation system for the migration ability of granular TPA has been established,and it was calculated that when there is no plugging expectation target,the comprehensive migration ability of small particle size TPA is stronger than that of large particle size TPA.This research provides theoretical foundation for the optimization of temporary plugging parameters.
文摘Cerebral or intracranial aneurysm is a leading cause of subarachnoid hemorrhage. It was initially treated with open surgical clipping but as rapid development of technology a less invasive endovascular coiling technique of aneurysm revolutionized the treatment. Due to tortuous anatomy of parent artery and complicated morphology and location of aneurysm there is ongoing challenge in the complete obliteration of aneurysms. To aid in the advances of treating aneurysm stent assisted endovascular coiling was introduced to give more scaffold support to parent artery and decrease events of coil protrusion from aneurysms. Many types and generation of stents were developed. One of the most recently introduced stent is low profile visualized intraluminal support (LVIS) stent. Due to its low profile nature it can be used in 0.017-inch inner diameter microcatheter and reach small and complex vessels providing high aneurysmal neck coverage which was not possible through other traditional stent. In addition, its braided design with tantalum strands and radiopaque markers make it more visible during stent placement and post procedure stent evaluation. Despite of many advantages of LVIS stent it is related to high rate of thromboembolic complications and technical complications. Aim of this review paper was to evaluate therapeutic safety, effectiveness and feasibility of LVIS stent in endovascular coil embolization of intracranial aneurysms.
基金financially supported by the National Natural Science Foundation of China (Nos. U1867206, 21906115)State Key Laboratory of Analytical Chemistry for Life Science (No. SKLACLS2014)+1 种基金the China Postdoctoral Science Foundation (No. 2020T130456)the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)。
文摘The development of uranyl ion detection technology has exhibited its significance in public security and environmental fields for the radioactivity and chemical toxicity of uranyl ion.The WHO standard of uranyl ion makes it necessary to develop highly sensitive uranyl rapid warning system in drinking water monitoring.Herein,a visualized rapid warning system for trace uranyl ion is carried out based on electrochemiluminescence(ECL) imaging technology to give an ultra-low limit of detection(LOD) and high selectivity.Amidoxime,a bi-functional group with both uranyl ion capturing and co-reactive functions,is modified on a conjugated polymer backbone with strong ECL signal to be prepared into three-in-one polymer nanoparticles(PNPs) with self-enhanced ECL property.The captured uranyl ion can enhance the ECL signal of PNPs via resonance energy transfer process to give the LOD as 0.5 ng/L,which is much lower than the known luminescent uranyl sensors.Furthermore,ECL imaging technology is introduced into realizing visualized uranyl rapid warning,and can be successfully applied on natural water samples.This study provides a novel strategy for uranyl rapid warning,and shows its potential meaning in public security and environmental fields.
基金the National Natural Science Foundation of China,Nos.51935009 and 51821093National key research and development project of China,No.2022YFB3303303+2 种基金Zhejiang University president special fund financed by Zhejiang province,No.2021XZZX008Zhejiang provincial key research and development project of China,Nos.2023C01060,LZY22E060002 and LZ22E050008The Ng Teng Fong Charitable Foundation in the form of ZJU-SUTD IDEA Grant,No.188170-11102.
文摘This study presents a robustness optimization method for rapid prototyping(RP)of functional artifacts based on visualized computing digital twins(VCDT).A generalized multiobjective robustness optimization model for RP of scheme design prototype was first built,where thermal,structural,and multidisciplinary knowledge could be integrated for visualization.To implement visualized computing,the membership function of fuzzy decision-making was optimized using a genetic algorithm.Transient thermodynamic,structural statics,and flow field analyses were conducted,especially for glass fiber composite materials,which have the characteristics of high strength,corrosion resistance,temperature resistance,dimensional stability,and electrical insulation.An electrothermal experiment was performed by measuring the temperature and changes in temperature during RP.Infrared thermographs were obtained using thermal field measurements to determine the temperature distribution.A numerical analysis of a lightweight ribbed ergonomic artifact is presented to illustrate the VCDT.Moreover,manufacturability was verified based on a thermal-solid coupled finite element analysis.The physical experiment and practice proved that the proposed VCDT provided a robust design paradigm for a layered RP between the steady balance of electrothermal regulation and manufacturing efficacy under hybrid uncertainties.
基金Project supported by the National Basic Research Program of China (Grant No. 2009 CB724100)the National Natural Science Foundation of China (Grant No. 11172326)
文摘In a Mach 3.8 wind tunnel, both instantaneous and time-averaged flow structures of different scales around a blunt double-cone with or without supersonic film cooling were visualized via nano-tracer planar laser scattering （NPLS）, which has a high spatiotemporal resolution. Three experimental cases with different injection mass flux rates were carried out. Many typical flow structures were clearly shown, such as shock waves, expansion fans, shear layers, mixing layers, and turbulent boundary layers. The analysis of two NPLS images with an interval of 5 us revealed the temporal evolution characteristics of flow structures. With matched pressures, the laminar length of the mixing layer was longer than that in the case with a larger mass flux rate, but the full covered region was shorter. Structures like K-H （Kelvin-Helmholtz） vortices were clearly seen in both flows. Without injection, the flow was similar to the supersonic flow over a backward- facing step, and the structures were relatively simpler, and there was a longer laminar region. Large scale structures such as hairpin vortices were visualized. In addition, the results were compared in part with the schlieren images captured by others under similar conditions.
文摘To work efficiently with DSS, most users need assistance in representation conversion, i. e., translating the specific outcome from the DSS into the universal language of visual. In generally, it is much easier to understand the results from the DSS if they are translated into charts, maps, and other scientific displays, because visualization exploits human natural ability to recognize and understand visual pattern. In this paper we discuss the concept of visualization for DSS. AniGraftool, a software system, is introduced as an example of Visualized User Interface for DSS.
基金Supported by the National Natural Science Foundation of China (41330319 and 42072174)Foundation of China University of Petroleum Beijing (2462020XKBH016)Fellowship of China Postdoctoral Science Foundation (2020M680030)。
文摘A micro-nano pore three-dimensional visualized real-time physical simulation of natural gas charging, in-situ pore-scale computation, pore network modelling, and apparent permeability evaluation theory were used to investigate laws of gas and water flow and their distribution, and controlling factors during the gas charging process in low-permeability(tight) sandstone reservoir. By describing features of gas-water flow and distribution and their variations in the micro-nano pore system, it is found that the gas charging in the low permeability(tight) sandstone can be divided into two stages, expansion stage and stable stage. In the expansion stage, the gas flows continuously first into large-sized pores then small-sized pores, and first into centers of the pores then edges of pores;pore-throats greater than 20 μm in radius make up the major pathway for gas charging. With the increase of charging pressure, movable water in the edges of large-sized pores and in the centers of small pores is displaced out successively. Pore-throats of 20-50 μm in radius and pore-throats less than 20 μm in radius dominate the expansion of gas charging channels at different stages of charging in turn, leading to reductions in pore-throat radius, throat length and coordination number of the pathway, which is the main increase stage of gas permeability and gas saturation. Among which, pore-throats 30-50 μm in radius control the increase pattern of gas saturation. In the stable stage, gas charging pathways have expanded to the maximum, so the pathways keep stable in pore-throat radius, throat length, and coordination number, and irreducible water remains in the pore system, the gas phase is in concentrated clusters, while the water phase is in the form of dispersed thin film, and the gas saturation and gas permeability tend stable. Connected pore-throats less than 20 μm in radius control the expansion limit of the charging pathways, the formation of stable gas-water distribution, and the maximum gas saturation. The heterogeneity of connected pore-throats affects the dynamic variations of gas phase charging and gas-water distribution. It can be concluded that the pore-throat configuration and heterogeneity of the micro-nanometer pore system control the dynamic variations of the low-permeability(tight) sandstone gas charging process and gas-water distribution features.
基金Supported by the Major State Basic Research Development Program of China(2012CB720500)the National Natural Science Foundation of China(6133301021276078)+3 种基金the National Science Fund for Outstanding Young Scholars(61222303)the Fundamental Research Funds for the Central Universities,Shanghai Rising-Star Program(13QH1401200)the Program for New Century Excellent Talents in University(NCET-10-0885)Shanghai R&D Platform Construction Program(13DZ2295300)
文摘Purified terephthalic acid(PTA) is an important chemical raw material. P-xylene(PX) is transformed to terephthalic acid(TA) through oxidation process and TA is refined to produce PTA. The PX oxidation reaction is a complex process involving three-phase reaction of gas, liquid and solid. To monitor the process and to improve the product quality, as well as to visualize the fault type clearly, a fault diagnosis method based on selforganizing map(SOM) and high dimensional feature extraction method, local tangent space alignment(LTSA),is proposed. In this method, LTSA can reduce the dimension and keep the topology information simultaneously,and SOM distinguishes various states on the output map. Monitoring results of PX oxidation reaction process indicate that the LTSA–SOM can well detect and visualize the fault type.
基金supported by the National Key Research and Development Project(2019YFA0708700)the National Natural Science Foundation of China(52104061)+2 种基金the project funded by China Postdoctoral Science Foundation(2020M682264)the Shandong Provincial Natural Science Foundation(ZR2021QE075)the Fundamental Research Funds for the Central Universities(20CX06090A)。
文摘Knowledge of migration and retention mechanisms of elastic gel particles(EGPs)in pore-throats is essential for the effective application of EGPs as a smart sweep improvement and profile control agent for enhanced oil recovery(EOR).The matching coefficient(defined as the ratio of particle size to pore-throat size)is used to investigate its influence on migration,retention and profile control performance of EGPs.A 1-D continuous pore-throat visualization model(PTVM),a 2-D heterogeneous PTVM and a 3-D heterogeneous core model were constructed and used to investigate pore-scale migration,retention and controlling mechanism of migration and retention characteristics on EGPs profile control.The results of the 1-D continuous PTVM indicated that while the matching coefficient was in the optimal range(i.e.,0.20-0.32),the EGPs could not only smoothly migrate to the deeper pore-throats,but also form stable retention in the pores to resist the erosion of injected water,which was conducive to the effective indepth profile control.The results of the 2-D heterogeneous PTVM verified that the sweep efficiency in low-permeability regions could be significantly improved by in-depth migration and stable retention of EGPs in the pore-throats with an optimal matching coefficient(0.29),which was much better than that in cases with a smaller matching coefficient(0.17)or an excessive matching coefficient(0.39).Moreover,the NMR displacement experiments of 3-D heterogeneous cores were carried out to simulate the EGPs profile control in actual reservoir porous media.Saturation images and T2 spectrum curves of crude oil showed that EOR in the low-permeability layer was highest(56.1%)using EGPs profile control with an optimal matching coefficient,attributing to the in-depth migration and stable retention of EGPs.
文摘With the social development, we are stepping into an information technology world. In such a world, our life is getting more and more diversified and rich because of e-business. E-business not only provides us convenience but also large amounts of business data. However, how shall we better store, manage and use these business data has become a major field being studied by e-business. With the rapid growth of data volume, the relational database system cannot meet the requirements of the current status. In this paper, focusing on the visualized analysis model of Hadoop business data, it analyzed the business data in terms of the visualized platform, database and analysis model etc. Depending on the analysis, offline-data analysis and data visualization for Hive database will be greatly improved, so that references and suggestions can be provided for the visualized analysis model of Hadoop business data.
文摘Objective: This study visualizes and analyzes the current status, hotspots, and frontiers of cardiac rehabilitation for patients with Coronary Heart Disease (CHD) over the past decade (2012.01-2022.12), and explores the future development trend and research direction of the CR for CHD patients in China. Methods: Relevant literature was searched, screened and downloaded from the Web of Science (WOS) database, and bibliometric and visualization analyses were performed using CiteSpace VI software. Results: Through the search and screening of related literature, 2443 English articles were finally included. Among them, most of the Chinese publishers were mainly universities and had less connection with each other, while the foreign publishers were mainly universities and medical institutions in the United States and Europe, and had close connection with each other. The research content of Chinese scholars mainly focuses on the assessment of patients’ life and psychological status, as well as the assessment of cardiac function. Foreign research focuses on physical training assessment, disease perception, etc. Conclusion: Through visualizing relevant research with CiteSpace VI software in the form of a knowledge map, the research frontiers and trends in the field of cardiac rehabilitation for coronary heart disease patients in China and abroad can be discovered more intuitively. Compared with foreign research, the development of cardiac rehabilitation for coronary heart disease patients in China is relatively slow and insufficient, and institutions lack cooperation. In the future, China should accelerate the connection between regions in the field of cardiac rehabilitation and develop a cardiac rehabilitation model suitable for coronary heart disease patients in China with its own characteristics.
文摘The 2020 TKK Science Award in I Life Sciences went to CAS member SHI Yigong,a distinguished structural biologist,for his achievements in:elucidating the yeast and human splicing cycle in atomic detail,which allows us to visually appreciate the dynamic process of the supermolecular machinery of spliceosome performing molecular surgery on the chain of a pre-message RNA(pre-mRNA)to give rise to a mature mRNA.
基金sponsored by the Major Projects of the National Natural Science Foundation of China(No.82293650,82293651)the Natural Science Foundation for Distinguished Young Scholars of Hebei Province,China(No.H2022206026).
文摘This study conducted a bibliometric and visualization analysis of 265 publications related to Gelsemium elegans Benth.(G.elegans)poisoning in China,retrieved from the China National Knowledge Infrastructure database covering the years 1995–2025.It systematically summarizes the current status,developmental trends,and research hotspots in this field.The results indicate that research on G.elegans poisoning in China has evolved through three distinct phases:An initial stage from 1995 to 2005,rapid growth from 2005 to 2015,and a peak period after 2016,with publication peaks in 2019 and 2022,reflecting growing scientific and public attention to this issue.Key research institutions are primarily concentrated at the Hunan Agricultural University and Fujian University of Traditional Chinese Medicine,representing the core centers in the field.Keyword co-occurrence and clustering analyses revealed“G.elegans,”“poisoning,”and“Gelsemium alkaloids”as central themes,with studies on the toxicological mechanisms of indole alkaloids being particularly prominent.Research directions span toxicological mechanisms,epidemiology,and clinical management.Funding analysis further showed that the National Natural Science Foundation of China has provided substantial support for this field.Overall,research on G.elegans poisoning has advanced considerably,especially regarding alkaloid constituents and their toxic mechanisms.However,translational research,early diagnosis,antidote development,and misuse prevention remain underexplored.Future studies should emphasize multidisciplinary collaboration to promote advances in clinical management,toxicological assessment,and public health prevention strategies related to G.elegans poisoning.
基金sponsored by the National Key Research and Devel-opment Program of China(2020YFA0908800)the National Natural Science Foundation of China(22134003)。
文摘In situ imaging diagnosis and precise treatment of deep tumor tissues are hotspots in life sciences and medical research.In recent years,using focused ultrasound to remotely control engineered bacteria for drug release has become one of the methods for precise in vivo drug delivery.However,non-visualized engineered bacteria pose challenges for precise control within the body.Therefore,there is an urgent need for an engineered bacterial vector capable of deep tissue imaging to precisely locate bacteria in vivo.Acoustic reporter genes(ARGs)are biological elements used for deep tissue imaging,with gene clusters over 8 kb.However,ARGs are often tested on plasmids,which hinders stable expression in vivo and limits the space for inserting components that regulate drug release.Therefore,we used the attenuated Salmonella typhimurium VNP20009,known for its tumor-targeting ability,as the chassis bacteria.By using CRISPR-Cas9 technology,we inserted ARGs into the genome and optimized the promoter strength and copy number for ARG expression,constructing ultrasound-visible engi-neered bacteria expressing gas vesicles on the genome.Additionally,by knocking out the stress protein gene htrA in VNP20009,we increased the maximum injection dose by tenfold and the tumor specificity by a hundredfold.The constructed ultrasound-visible engineered bacteria can stably synthesize gas vesicles and output ultrasound signals while directly carrying drug plasmids for tumor therapy.Our research provides an effective vector for diagnosis and precise treatment.
基金financially supported by the National Natural Science Foundation of China(Grant Nos.12132016,12202435,12427802,and 52321003)the Fundamental Research Funds for the Central Universities(Grant No.WK2090000060)the USTC Center for Micro and Nanoscale Research and Fabrication.
文摘The development of intelligent textiles that integrate impact protection with real-time sensing capabilities is of critical importance for next-generation wearable protective systems.Despite extensive usage of conventional protective films/elastomers,their inherent planar geometries compromise wearing comfort,and the universal absence of real-time impact detection/location capabilities restricts application prospects.To address these challenges,an intelligent shear-stiffening-based mechanoluminescent fiber(ML-TPS)is developed through integrated wet-spinning and coating technology.This fiber combines a shear-stiffening polymer core with a ZnS:Cu/polydimethylsiloxane(PDMS)mechanoluminescent coating,synergistically enabling excellent impact resistance and spatiotemporal force visualization.The resultant 4 mm-thick ML-TPS fabric maintains exceptional flexibility,breathability,and high impact energy dissipation(efficiency>90%)while demonstrating rapid damage localization(response time<6 ms)and quantitative impact assessment(R2=0.95 linear correlation),surpassing conventional materials in temporal resolution.The fabricated visual sensing matrix enables visual localization,showing unique advantages in scenarios requiring rapid impact response,such as sports protection and personal safety.Finally,the multi-scenario applicability of ML-TPS fibers is demonstrated through human motion monitoring and underwater warning validation.This work provides a new paradigm for developing active protection-type intelligent wearable systems.
基金National Key R&D Program of China(2021YFA0717600)National Natural Science Foundation of China(NSFC No.62305022,NSFC No.62035004,NSFC No.U22A2081)Young Elite Scientists Sponsorship Program by CAST(No.YESS20200163).
文摘Biological visions have inspired the development of artificial vision systems with diverse visual functional traits,however,the detected wavelength is only in visible light between 0.4 and 0.78μm,restricting their applications.Snakes generate a thermal image of animals due to pit organs for detecting and converting infrared,allowing them to accurately target predators or prey even under darkness.Inspired by natural infrared visualized snakes,we propose artificial vision systems with CMOS sensors-integrated upconverters to break visible light limitations to realize 3840×2160 ultra-high-resolution short-wave infrared(SWIR)and mid-wave infrared(MWIR)visualization imaging for the first time.Through colloidal quantum dot barrier heterojunction architecture design of infrared detecting units and the introduction of co-hosted emitting units,the luminance and upconversion efficiency reach up to 6388.09 cd m^(−2) and 6.41%for SWIR,1311.64 cd m^(−2) and 4.06%for MWIR at room temperature.Our artificial vision systems broaden a wide spectrum of applications within infrared,such as night vision,agricultural science,and industry inspection,marking a significant advance in bioartificial vision.
基金supported by the National Natural Science Foundation of China(nos.21788102 and 21525417)the Natural Science Foundation of Guangdong Province(nos.2019B030301003 and 2016A030312002)the Innovation and Technology Commission of Hong Kong(no.ITC-CNERC14S01).
文摘In comparing with traditional polymer degradation toward unknown or unvalued products(i.e.,downcycling),new methods to degrade polymers into monomers or high-value-added materials(i.e.,upcycling)are preferable for sustainable developments.In this work,CO_(2)-based linear and cross-linked unsaturated polyesters,that is,poly(alkynoate)s,were successfully degraded by benzamidine into diols and high-value-added pyrimidone or imidazolone derivatives through controlled reaction conditions.The degradation process could be visualized under UV light via gradually increased luminescent intensity of the reaction mixtures and fluorescence patterns could be generated through microcontact printing of the stamp on the thin films of poly(alkynoate)s.Moreover,the pyrimidone derivatives could be applied in bioimaging and inhibiting bacteria,and the imidazolone derivatives could be used for the detection and recovery of gold(III)ions from electronic waste and serve as the fluorescent sensor to detect in situ-generated amines from food spoilage.Thus,this work presents a visualized and high value productselective degradation to solve the end-of-life issue of polymers.
基金The author would like to acknowledge the National Science Fund for Distinguished Young Scholars(21425624)the National Natural Science Foundation of China(21476026,21376028).
文摘Small non-coding RNAs(sRNAs)have received much attention in recent years due to their unique biological properties,which can efficiently and specifically tune target gene expressions in bacteria.Inspired by natural sRNAs,recent works have proposed the use of artificial sRNAs(asRNAs)as genetic tools to regulate desired gene that has been applied in several fields,such as metabolic engineering and bacterial physiology studies.However,the rational design of asRNAs is still a challenge.In this study,we proposed structure and length as two criteria to implement rational visualized and precise design of asRNAs.T7 expression system was one of the most useful recombinant protein expression systems.However,it was deeply limited by the formation of inclusion body.To settle this problem,we designed a series of asRNAs to inhibit the T7 RNA polymerase(Gene1)expression to balance the rate between transcription and folding of recombinant protein.Based on the heterologous expression of Aspergillus oryzae Li-3 glucuronidase in E.coli,the asRNA-antigene1-17bp can effectively decrease the inclusion body and increase the enzyme activity by 169.9%.
