AIM:To investigate the postnatal development of parvalbumin(PV)-positive gamma-aminobutyric acid(GABA)interneurons and the co-expression of perineuronal nets(PNNs)and PV in the visual cortex of rats,as well as the reg...AIM:To investigate the postnatal development of parvalbumin(PV)-positive gamma-aminobutyric acid(GABA)interneurons and the co-expression of perineuronal nets(PNNs)and PV in the visual cortex of rats,as well as the regulatory effects of fluoxetine(FLX)treatment and binocular form deprivation(BFD)on these indices.METHODS:Wistar rats were assigned to three experimental cohorts:1)Age-related groups:postnatal week(PW)1,PW3,PW5,PW7,and PW9;2)FLX treatment duration groups:FLX 0W,FLX 2W,FLX 4W,FLX 6W,and FLX 8W;3)Intervention groups:control(Cont),FLX,BFD,and BFD+FLX.The levels of PNNs,PV,and PNNs/PV coexpression in the visual cortex were detected and analyzed.RESULTS:The density of PV-positive cells and the coexpression of PNNs and PV increased gradually with the maturation of the visual cortex(b=0.960,P<0.01).The ratio of PV-positive cells surrounded by PNNs to total PV-positive cells(PNNs+/PV+/total PV+)was significantly decreased in the FLX 4W group(χ^(2)=9.03,P=0.003).There was no significant difference in the PNNs+/PV+/total PV+ratio between the FLX and BFD groups(χ^(2)=1.08,P=0.161),but a significant difference was observed between the BFD+FLX group and the BFD group(χ^(2)=5.82,P<0.01).CONCLUSION:The number of PV-positive neurons and PNNs-surrounded PV neurons in the rat visual cortex increases postnatally and reaches adult levels by postnatal week 7.Chronic FLX treatment downregulates these expressions.Combined 4-week FLX treatment and BFD exerts a more significant inhibitory effect on the PNNs+/PV+/total PV+ratio than either intervention alone.展开更多
Amblyopia is a neurodevelopmental vision disorder resulting from abnormal visual input during the critical period of visual development,such as strabismus,uncorrected anisometropia,high refractive errors,and form depr...Amblyopia is a neurodevelopmental vision disorder resulting from abnormal visual input during the critical period of visual development,such as strabismus,uncorrected anisometropia,high refractive errors,and form deprivation.It is frequently associated with reduced visual acuity and deficits in binocular vision.Traditional occlusion therapy for amblyopia has typically been restricted to infants and young children during the critical period of visual development,as it is believed to be ineffective for older children and adults due to the decreased plasticity of the mature brain.Our research group has concentrated on pivotal scientific issues in amblyopia,including quantitative methods for detecting binocular vision,especially interocular visual suppression,the mechanisms underlying binocular vision impairment in amblyopia,treatment methods and their evaluations for amblyopia,and visual plasticity and its neural mechanisms in amblyopia.This paper summarizes the visual mechanisms and treatment modalities of amblyopia based on our research and both domestic and foreign sources,while also looking forward to the future development of this field in light of existing problems.展开更多
Objective:To report a case of anisometropic amblyopia reversal as a result of branch retinal vein occlusion (BRVO) occurring in the sound fellow eye.Methods:An adult patient with childhood anisometropic amblyopia was ...Objective:To report a case of anisometropic amblyopia reversal as a result of branch retinal vein occlusion (BRVO) occurring in the sound fellow eye.Methods:An adult patient with childhood anisometropic amblyopia was found to exhibit gradually visual acuity improvement in the amblyopic eye as the result of decreased visual acuity in the sound fellow eye.Results:Four years after the BRVO,the patient subsequently regained normal vision in the previously amblyopic eye.Conclusion:Visual plasticity is preserved in adults but may require special or stronger stimulation and possibly longer treatment time than during childhood.展开更多
基金Supported by the Suzhou Science and Technology Bureau(No.SKY2023175)the Project of State Key Laboratory of Radiation Medicine and Protection+6 种基金Soochow University(No.GZK1202309)the Advantage Subject Lifting Project(No.XKTJ-XK202412)the Suzhou Science and Education for Strengthening Healthcare(No.MSXM2024010)the Suzhou Medical Key Supported Disciplines(No.SZFCXK202118)the Youth Scientific Research Fund Project of Kunshan Hospital of Traditional Chinese Medicine(No.2024QNJJ06)the Postgraduate Research&Practice Innovation Program of Jiangsu Province(No.SJCX23_1673)the Undergraduate Training Program for Innovation and Entrepreneurship,Soochow University(No.202310285162Y).
文摘AIM:To investigate the postnatal development of parvalbumin(PV)-positive gamma-aminobutyric acid(GABA)interneurons and the co-expression of perineuronal nets(PNNs)and PV in the visual cortex of rats,as well as the regulatory effects of fluoxetine(FLX)treatment and binocular form deprivation(BFD)on these indices.METHODS:Wistar rats were assigned to three experimental cohorts:1)Age-related groups:postnatal week(PW)1,PW3,PW5,PW7,and PW9;2)FLX treatment duration groups:FLX 0W,FLX 2W,FLX 4W,FLX 6W,and FLX 8W;3)Intervention groups:control(Cont),FLX,BFD,and BFD+FLX.The levels of PNNs,PV,and PNNs/PV coexpression in the visual cortex were detected and analyzed.RESULTS:The density of PV-positive cells and the coexpression of PNNs and PV increased gradually with the maturation of the visual cortex(b=0.960,P<0.01).The ratio of PV-positive cells surrounded by PNNs to total PV-positive cells(PNNs+/PV+/total PV+)was significantly decreased in the FLX 4W group(χ^(2)=9.03,P=0.003).There was no significant difference in the PNNs+/PV+/total PV+ratio between the FLX and BFD groups(χ^(2)=1.08,P=0.161),but a significant difference was observed between the BFD+FLX group and the BFD group(χ^(2)=5.82,P<0.01).CONCLUSION:The number of PV-positive neurons and PNNs-surrounded PV neurons in the rat visual cortex increases postnatally and reaches adult levels by postnatal week 7.Chronic FLX treatment downregulates these expressions.Combined 4-week FLX treatment and BFD exerts a more significant inhibitory effect on the PNNs+/PV+/total PV+ratio than either intervention alone.
基金supported by the National Natural Science Foundation of China(82271115,82471115)the Natural Science Foundation Team Project of Guangdong Province Grant(2015A030312016).
文摘Amblyopia is a neurodevelopmental vision disorder resulting from abnormal visual input during the critical period of visual development,such as strabismus,uncorrected anisometropia,high refractive errors,and form deprivation.It is frequently associated with reduced visual acuity and deficits in binocular vision.Traditional occlusion therapy for amblyopia has typically been restricted to infants and young children during the critical period of visual development,as it is believed to be ineffective for older children and adults due to the decreased plasticity of the mature brain.Our research group has concentrated on pivotal scientific issues in amblyopia,including quantitative methods for detecting binocular vision,especially interocular visual suppression,the mechanisms underlying binocular vision impairment in amblyopia,treatment methods and their evaluations for amblyopia,and visual plasticity and its neural mechanisms in amblyopia.This paper summarizes the visual mechanisms and treatment modalities of amblyopia based on our research and both domestic and foreign sources,while also looking forward to the future development of this field in light of existing problems.
文摘Objective:To report a case of anisometropic amblyopia reversal as a result of branch retinal vein occlusion (BRVO) occurring in the sound fellow eye.Methods:An adult patient with childhood anisometropic amblyopia was found to exhibit gradually visual acuity improvement in the amblyopic eye as the result of decreased visual acuity in the sound fellow eye.Results:Four years after the BRVO,the patient subsequently regained normal vision in the previously amblyopic eye.Conclusion:Visual plasticity is preserved in adults but may require special or stronger stimulation and possibly longer treatment time than during childhood.
