Emerging and powerful genome editing tools,particularly CRISPR/Cas9,are facilitating functional genomics research and accelerating crop improvement(Jiang et al.2021;Cao et al.2023;Chen C et al.2023;Liu et al.2023a).Ho...Emerging and powerful genome editing tools,particularly CRISPR/Cas9,are facilitating functional genomics research and accelerating crop improvement(Jiang et al.2021;Cao et al.2023;Chen C et al.2023;Liu et al.2023a).However,the detection and screening of transgenic lines remain major bottlenecks,being time-consuming,labor-intensive,and inefficient during transformation and subsequent mutation identification.A simple and efficient visual marker system plays a critical role in addressing these challenges.Recent studies demonstrated that the GmW1 and RUBY reporter systems were used to obtain visual transgenic soybean(Glycine max) plants(Chen L et al.2023;Chen et al.2024).展开更多
The massive diffusion of smartphones, the growing interest in wearable devices and the Internet of Things, and the exponential rise of location based services（LBSs） have made the problem of localization and navigati...The massive diffusion of smartphones, the growing interest in wearable devices and the Internet of Things, and the exponential rise of location based services（LBSs） have made the problem of localization and navigation inside buildings one of the most important technological challenges of recent years. Indoor positioning systems have a huge market in the retail sector and contextual advertising; in addition, they can be fundamental to increasing the quality of life for citizens if deployed inside public buildings such as hospitals, airports, and museums.Sometimes, in emergency situations, they can make the difference between life and death. Various approaches have been proposed in the literature. Recently, thanks to the high performance of smartphones＇ cameras, marker-less and marker-based computer vision approaches have been investigated. In a previous paper, we proposed a technique for indoor localization and navigation using both Bluetooth low energy（BLE） and a 2D visual marker system deployed into the floor. In this paper, we presented a qualitative performance evaluation of three 2D visual markers, Vuforia,Ar Uco marker, and AprilT ag, which are suitable for real-time applications. Our analysis focused on specific case study of visual markers placed onto the tiles, to improve the efficiency of our indoor localization and navigation approach by choosing the best visual marker system.展开更多
The material flow in friction stir welded 2014 Al alloy has been investigated using a marker insert technique （MIT）. Results of the flow visualization show that the material flow is asymmetrical during the friction ...The material flow in friction stir welded 2014 Al alloy has been investigated using a marker insert technique （MIT）. Results of the flow visualization show that the material flow is asymmetrical during the friction stir welding （FSW） process and there are also significant differences in the flow patterns observed on advancing side and retreating side. On advancing side, some material transport forward and some move backward, but on retreating side, material only transport backward. At the top surface of the weld, significant material transport forward due to the action of the rotating tool shoulder. Combining the data from all the markers, a three-dituensional flow visualization, similar to the 3D image reconstruction technique, was obtained. The three-dimensional plot gives the tendency chart of material flow in friction stir welding process and from the plot it can be seen that there is a vertical, circular motion around the longitudinal axis of the weld. On the advancing side of the weld, the material is pushed downward but on the retreating side, the material is pushed toward the crown of the weld. The net result of the two relative motions in both side of the advancing and the retreating is that a circular motion comes into being. Comparatively, the material flow around the longitudinal axis is a secondary motion.展开更多
使用中国知网和Web of Science核心合集数据库,借助CiteSpace软件对2010—2023年的立场标记语相关文献进行分析,并绘制了知识图谱。研究发现:国外相关领域的发文量高于国内;国内外研究者间及国内研究机构间尚未形成紧密合作,国外部分研...使用中国知网和Web of Science核心合集数据库,借助CiteSpace软件对2010—2023年的立场标记语相关文献进行分析,并绘制了知识图谱。研究发现:国外相关领域的发文量高于国内;国内外研究者间及国内研究机构间尚未形成紧密合作,国外部分研究机构已开始合作发文;国内研究热点包括语料库研究和对比分析方法、学术写作中的立场标记语使用及其人际功能;国外研究热点为多语言中的立场标记语、非言语立场表达及多元语料研究。针对立场标记语研究的现状与不足,提出以下建议:加强研究者和机构间的合作与交流,开展跨文化、跨语言研究,关注非言语立场表达,利用多元语料进行跨学科研究,加强宏观理论思辨、夯实微观实证研究。展开更多
基金supported by the Jilin Science and Technology Development Program,China (20240602032RC)the Jilin Agricultural Science and Technology Innovation Project,China (CXGC2024ZD001)+1 种基金the Jilin Agricultural Science and Technology Innovation Project,China (CXGC2024ZY012)the Jilin Province Development and Reform Commission-Project for Improving the Independent Innovation Capacity of Major Grain Crops,China (2024C002)。
文摘Emerging and powerful genome editing tools,particularly CRISPR/Cas9,are facilitating functional genomics research and accelerating crop improvement(Jiang et al.2021;Cao et al.2023;Chen C et al.2023;Liu et al.2023a).However,the detection and screening of transgenic lines remain major bottlenecks,being time-consuming,labor-intensive,and inefficient during transformation and subsequent mutation identification.A simple and efficient visual marker system plays a critical role in addressing these challenges.Recent studies demonstrated that the GmW1 and RUBY reporter systems were used to obtain visual transgenic soybean(Glycine max) plants(Chen L et al.2023;Chen et al.2024).
文摘The massive diffusion of smartphones, the growing interest in wearable devices and the Internet of Things, and the exponential rise of location based services（LBSs） have made the problem of localization and navigation inside buildings one of the most important technological challenges of recent years. Indoor positioning systems have a huge market in the retail sector and contextual advertising; in addition, they can be fundamental to increasing the quality of life for citizens if deployed inside public buildings such as hospitals, airports, and museums.Sometimes, in emergency situations, they can make the difference between life and death. Various approaches have been proposed in the literature. Recently, thanks to the high performance of smartphones＇ cameras, marker-less and marker-based computer vision approaches have been investigated. In a previous paper, we proposed a technique for indoor localization and navigation using both Bluetooth low energy（BLE） and a 2D visual marker system deployed into the floor. In this paper, we presented a qualitative performance evaluation of three 2D visual markers, Vuforia,Ar Uco marker, and AprilT ag, which are suitable for real-time applications. Our analysis focused on specific case study of visual markers placed onto the tiles, to improve the efficiency of our indoor localization and navigation approach by choosing the best visual marker system.
文摘The material flow in friction stir welded 2014 Al alloy has been investigated using a marker insert technique （MIT）. Results of the flow visualization show that the material flow is asymmetrical during the friction stir welding （FSW） process and there are also significant differences in the flow patterns observed on advancing side and retreating side. On advancing side, some material transport forward and some move backward, but on retreating side, material only transport backward. At the top surface of the weld, significant material transport forward due to the action of the rotating tool shoulder. Combining the data from all the markers, a three-dituensional flow visualization, similar to the 3D image reconstruction technique, was obtained. The three-dimensional plot gives the tendency chart of material flow in friction stir welding process and from the plot it can be seen that there is a vertical, circular motion around the longitudinal axis of the weld. On the advancing side of the weld, the material is pushed downward but on the retreating side, the material is pushed toward the crown of the weld. The net result of the two relative motions in both side of the advancing and the retreating is that a circular motion comes into being. Comparatively, the material flow around the longitudinal axis is a secondary motion.
文摘使用中国知网和Web of Science核心合集数据库,借助CiteSpace软件对2010—2023年的立场标记语相关文献进行分析,并绘制了知识图谱。研究发现:国外相关领域的发文量高于国内;国内外研究者间及国内研究机构间尚未形成紧密合作,国外部分研究机构已开始合作发文;国内研究热点包括语料库研究和对比分析方法、学术写作中的立场标记语使用及其人际功能;国外研究热点为多语言中的立场标记语、非言语立场表达及多元语料研究。针对立场标记语研究的现状与不足,提出以下建议:加强研究者和机构间的合作与交流,开展跨文化、跨语言研究,关注非言语立场表达,利用多元语料进行跨学科研究,加强宏观理论思辨、夯实微观实证研究。
