[ Objective] To investigate the in vitro bacteriostatic activity of Chinese herbal medicines （ rhubarb, Baikal skullcap root, honeysuckle, eucommia bark and Chinese date） and their compounds against two common patho...[ Objective] To investigate the in vitro bacteriostatic activity of Chinese herbal medicines （ rhubarb, Baikal skullcap root, honeysuckle, eucommia bark and Chinese date） and their compounds against two common pathogens of Scylla serrata （ Vibrio parahaemolyticus and Aeromonas hydrophila）. [Method] Drug sensitivity of the bacteria was determined by paper method. Minimum inhibitory concentration （MIC） and minimum bactericidal concentration （MBC） were determined by dilution method and plate method, respectively. [Result] The bacteriostatic order of single medicines on V. parahaemolyticus were Baikal skullcap root 〉 honeysuckle 〉 rhubarb 〉 Chinese date 〉 eucommia bark. The bacteriostatic order of the compounds were A =B = C 〉 D. Among these medicines, the Baikal skullcap root and the compounds A, B, C and D had better bactericidal effects on V. parahaemolyticus. The bacteriostatic order of single medicines on A. hydrophila was rhubarb 〉 Baikal skullcap root 〉 honeysuckle 〉 Chinese date 〉 eucommia bark. The bacteriostatic order of the compounds were A 〉 B 〉 C = D, while the rhubarb and the compound C had better bactericidal effects on A. hydrophila. [ Conclusion] The Chinese herbal medicines including rhubarb, Baikal skullcap root, honeysuckle, eucommia bark and Chinese date have good bacteriostatic effects on V. parahaemolyticus and A. hydrophila, and they can be used to treat bacterial infection in Scylla serrata.展开更多
基金funded by the Transformation Project of Scientific Achievements in Guangdong Colleges (cgzhzd0812)
文摘[ Objective] To investigate the in vitro bacteriostatic activity of Chinese herbal medicines （ rhubarb, Baikal skullcap root, honeysuckle, eucommia bark and Chinese date） and their compounds against two common pathogens of Scylla serrata （ Vibrio parahaemolyticus and Aeromonas hydrophila）. [Method] Drug sensitivity of the bacteria was determined by paper method. Minimum inhibitory concentration （MIC） and minimum bactericidal concentration （MBC） were determined by dilution method and plate method, respectively. [Result] The bacteriostatic order of single medicines on V. parahaemolyticus were Baikal skullcap root 〉 honeysuckle 〉 rhubarb 〉 Chinese date 〉 eucommia bark. The bacteriostatic order of the compounds were A =B = C 〉 D. Among these medicines, the Baikal skullcap root and the compounds A, B, C and D had better bactericidal effects on V. parahaemolyticus. The bacteriostatic order of single medicines on A. hydrophila was rhubarb 〉 Baikal skullcap root 〉 honeysuckle 〉 Chinese date 〉 eucommia bark. The bacteriostatic order of the compounds were A 〉 B 〉 C = D, while the rhubarb and the compound C had better bactericidal effects on A. hydrophila. [ Conclusion] The Chinese herbal medicines including rhubarb, Baikal skullcap root, honeysuckle, eucommia bark and Chinese date have good bacteriostatic effects on V. parahaemolyticus and A. hydrophila, and they can be used to treat bacterial infection in Scylla serrata.
