Objective:To evaluate the hepatoprotective effects of the ethanol fraction of Verbascum thapsus L.(EFVT)against CCl4-induced liver injury and elucidate its underlying mechanisms.Methods:The assessment of antioxidant p...Objective:To evaluate the hepatoprotective effects of the ethanol fraction of Verbascum thapsus L.(EFVT)against CCl4-induced liver injury and elucidate its underlying mechanisms.Methods:The assessment of antioxidant properties and cell viability was conducted using the 2,2-diphenyl-1-picrylhydrazyl assay and HepG2 cells,respectively.The in vivo hepatoprotective efficacy of EFVT was evaluated in a rat model of carbon tetrachloride(CCl4)-induced liver injury by determining biochemical parameters,and oxidative stress-and inflammation-related markers.Gas chromatography-mass spectrometry was also employed for the qualitative analysis of its phytochemical composition.Results:GC-MS analysis of EFVT revealed the presence of several bioactive compounds such as 3 methyl mannoside and 9,12-octadecadienoic acid.Oral administration of EFVT significantly mitigated CCl4-induced liver injury,as evidenced by reduced levels of total bilirubin,alkaline phosphatase,alanine aminotransferase,aspartate aminotransferase,and malondialdehyde,boosted activities of catalase and superoxide dismutase,as well as enhanced glutathione levels.Histopathological examinations indicated EFVT restored abnormal liver architecture and reduced inflammation.Additionally,EFVT substantially downregulated the mRNA levels of IL-6,IL-1β,TNF-α,and NF-κB,and upregulated IL-10 expression.Conclusions:These findings underscore the therapeutic potential of EFVT in ameliorating liver damage associated with oxidative stress,providing scientific validation for its traditional utilization in ethnomedicine.展开更多
A great mullein (Verbascum thapsus L.) was a famous species in Scrophulariaceae family. It was generally used as herbal medicine. Explants of V. thapsus (leaves and petioles) were cultured in vitro on Murashige and Sk...A great mullein (Verbascum thapsus L.) was a famous species in Scrophulariaceae family. It was generally used as herbal medicine. Explants of V. thapsus (leaves and petioles) were cultured in vitro on Murashige and Skoog (MS) medium for shoot proliferation.Plantlet explants were cultured on MS medium supplemented with combination of Benzyl adenine (BA) and Naphthalene acetic acid (NAA) for callus induction. The best fresh and dry weight of callus formation was achieved using 0.5 mg/l BA. Quantitative analyses with High-performance liquid chromatography (HPLC) showed the content of phenols like Coumarin, Eugenol and Thymol were relatively low in leaves of mother plant, (10, 41, 310 ppm) respectively. The addition of different concentrations of amino acids as a precursor adding separately to the tissue culture medium led to raise the accumulation levels of phenolic compounds in callus tissue. Generally, the enhancement of accumulation depended on the type of amino acids and their concentration. The results showed 150 mg/l of Proline encouraged production of Comarin to 2752%, while 50 mg/l of Proline promoted accumulation of Eugenol to 290%. Whilst 150 mg/l of Tryptophan increased production of Thymol to 390%, in comparison with mother plant.展开更多
文摘Objective:To evaluate the hepatoprotective effects of the ethanol fraction of Verbascum thapsus L.(EFVT)against CCl4-induced liver injury and elucidate its underlying mechanisms.Methods:The assessment of antioxidant properties and cell viability was conducted using the 2,2-diphenyl-1-picrylhydrazyl assay and HepG2 cells,respectively.The in vivo hepatoprotective efficacy of EFVT was evaluated in a rat model of carbon tetrachloride(CCl4)-induced liver injury by determining biochemical parameters,and oxidative stress-and inflammation-related markers.Gas chromatography-mass spectrometry was also employed for the qualitative analysis of its phytochemical composition.Results:GC-MS analysis of EFVT revealed the presence of several bioactive compounds such as 3 methyl mannoside and 9,12-octadecadienoic acid.Oral administration of EFVT significantly mitigated CCl4-induced liver injury,as evidenced by reduced levels of total bilirubin,alkaline phosphatase,alanine aminotransferase,aspartate aminotransferase,and malondialdehyde,boosted activities of catalase and superoxide dismutase,as well as enhanced glutathione levels.Histopathological examinations indicated EFVT restored abnormal liver architecture and reduced inflammation.Additionally,EFVT substantially downregulated the mRNA levels of IL-6,IL-1β,TNF-α,and NF-κB,and upregulated IL-10 expression.Conclusions:These findings underscore the therapeutic potential of EFVT in ameliorating liver damage associated with oxidative stress,providing scientific validation for its traditional utilization in ethnomedicine.
文摘A great mullein (Verbascum thapsus L.) was a famous species in Scrophulariaceae family. It was generally used as herbal medicine. Explants of V. thapsus (leaves and petioles) were cultured in vitro on Murashige and Skoog (MS) medium for shoot proliferation.Plantlet explants were cultured on MS medium supplemented with combination of Benzyl adenine (BA) and Naphthalene acetic acid (NAA) for callus induction. The best fresh and dry weight of callus formation was achieved using 0.5 mg/l BA. Quantitative analyses with High-performance liquid chromatography (HPLC) showed the content of phenols like Coumarin, Eugenol and Thymol were relatively low in leaves of mother plant, (10, 41, 310 ppm) respectively. The addition of different concentrations of amino acids as a precursor adding separately to the tissue culture medium led to raise the accumulation levels of phenolic compounds in callus tissue. Generally, the enhancement of accumulation depended on the type of amino acids and their concentration. The results showed 150 mg/l of Proline encouraged production of Comarin to 2752%, while 50 mg/l of Proline promoted accumulation of Eugenol to 290%. Whilst 150 mg/l of Tryptophan increased production of Thymol to 390%, in comparison with mother plant.
