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金黄色葡萄球菌所致肺部感染的耐药特点及其Panton-Valentine杀白细胞素基因的携带状况分析 被引量:6
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作者 张雪青 陈增强 +1 位作者 黄金伟 余方友 《中国微生态学杂志》 CAS CSCD 2008年第1期51-53,共3页
目的分析金黄色葡萄球菌所致肺部感染的耐药性特点及其Panton-Valentine杀白细胞素基因的携带状况。方法回顾性调查了温州医学院第一附属医院2005年1月至2006年1月医院感染的金黄色葡萄球菌所致肺部感染患者132例,对其体外药敏试验进行... 目的分析金黄色葡萄球菌所致肺部感染的耐药性特点及其Panton-Valentine杀白细胞素基因的携带状况。方法回顾性调查了温州医学院第一附属医院2005年1月至2006年1月医院感染的金黄色葡萄球菌所致肺部感染患者132例,对其体外药敏试验进行分析;并利用多重PCR检测其PVL基因,应用多位点基因序列分型(multilocus sequence typing,MLST)技术对PVL基因阳性的菌株进行序列分型。耐甲氧西林金黄色葡萄球菌(methicillin-resistant Staphylococcus aureus,MRSA)的SCCmec基因分型采用多重聚合酶链反应。结果致肺部感染的132株金黄色葡萄球菌的耐药现象较为严重,仅对万古霉素、呋喃妥因及复方新诺明等药物的敏感率较高;其中经多重PCR筛选出10株携带PVL基因的金葡菌,全部为MRSA菌株,3株为ST239-SCCⅢ,2株为ST398-SCCmecⅢ,2株为ST398-SCCmecⅣ,ST25-SCCmecⅢ、ST59-SCCmecⅠ和ST88-SCCmecⅢ各1株。结论肺部感染的金黄色葡萄球菌对多种抗生素耐药,呈多重耐药性;其携带PVL基因占一定比例。 展开更多
关键词 金黄色葡萄球菌 肺部感染 耐药 Panton—Valentine杀白细胞素基因 耐甲氧西林金黄色葡萄球菌
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儿科Panton-Valentine杀白细胞素基因阳性耐甲氧西林金黄色葡萄球菌分子特征及Panton-Valentine杀白细胞素基因多态性和耐药特征
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作者 孙静 李向梅 +7 位作者 孙明姣 王丽娟 刘颖超 王传清 邓秋莲 刘岚 杨永弘 沈叙庄 《实用儿科临床杂志》 CAS CSCD 北大核心 2012年第22期1707-1711,共5页
目的研究儿童感染Panton-Valentine杀白细胞素(PVL)基因阳性耐甲氧西林金黄色葡萄球菌(MRSA)分离株分子特征、PVL基因序列变异情况及耐药特征。方法对PVL阳性MRSA感染患儿临床资料进行回顾性调查,分析病例临床特征;采用PCR联合测序对MRS... 目的研究儿童感染Panton-Valentine杀白细胞素(PVL)基因阳性耐甲氧西林金黄色葡萄球菌(MRSA)分离株分子特征、PVL基因序列变异情况及耐药特征。方法对PVL阳性MRSA感染患儿临床资料进行回顾性调查,分析病例临床特征;采用PCR联合测序对MRSA临床分离株进行多位点序列分型(MLST);并应用分段PCR扩增PVL基因,拼接序列与日本ST30 CA-MRSA菌株NN1的PVL序列进行比对,分析序列变异与STs分型之间的关系,构建系统树对PVL基因的系统发生多样性进行分析;采用琼脂稀释法进行16种抗生素的体外药物敏感试验。结果在73例PVL阳性MRSA感染病例中:皮肤软组织感染35例(47.9%),肺炎27例(37.0%),其他严重感染及健康儿童分离株中也检测到PVL基因;社区感染55例(75.3%)。感染菌株MLST分型共有9种,最常见的3种为ST59、ST910、ST338,分别占53.4%、17.8%、9.6%。PVL序列检测及比对结果:共发现9个核苷酸位点变异,其中3个为非同义突变(16 C→A,62 C→T,527 A→G),其余均为同义突变;有9种不同的单体型序列,各STs分型在9种序列中呈散在分布;所研究的MRSA 71株为H型,仅2株R型。PVL阳性MRSA菌株对红霉素、克林霉素、头孢呋辛耐药率高达94.5%、86.3%和82.2%。结论 PVL阳性MRSA分离株来源广泛,以皮肤软组织感染和肺炎为主;分子分型以ST59型最为常见;lukSF-PV序列高度保守,序列变异主要为H型,R型罕见。 展开更多
关键词 金黄色葡萄球菌 甲氧西林耐药 Panton—Valentine杀白细胞素 等位基因变异
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浅谈计算机病毒及防护 被引量:1
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作者 姜峰 《今日科苑》 2009年第4期193-193,共1页
本文首先分析了计算机病毒的类型及特点,并对计算机病毒注入进行了技术分析,研究出了计算机病毒的主要防护方法。
关键词 计算机病毒 防护措施 “美丽杀手”(Meiissa)病毒 尼姆亚变种w 情人节(vbs.Valentin)病毒
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Valentine’s Day
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作者 王宗文 《语言教育》 2002年第2期15-17,共3页
Every February,across the country,candy,flowers,and gifts are exchanged between loved ones,all in the name of St.Valentine.But who is thismysterious saint and why do we celebrate thisholiday?
关键词 FLOWERS exchanged celebrate GREETING BEGIN Valentine s DAY FERTILITY himself TODAY
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用于瞬态高功率脉冲辐射的超宽带天线设计 被引量:3
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作者 毕岚 薛谦忠 席宝坤 《强激光与粒子束》 EI CAS CSCD 北大核心 2018年第8期62-66,共5页
基于Valentine天线的辐射原理,设计出可发射峰值电压610kV、频谱范围0.2~2GHz高功率脉冲的超宽带天线。利用油作为绝缘介质过渡巴伦可使天线直接加载于50Ω的同轴波导上;通过优化填充介质及天线拓扑结构提高了耐压能力及辐射特性。仿真... 基于Valentine天线的辐射原理,设计出可发射峰值电压610kV、频谱范围0.2~2GHz高功率脉冲的超宽带天线。利用油作为绝缘介质过渡巴伦可使天线直接加载于50Ω的同轴波导上;通过优化填充介质及天线拓扑结构提高了耐压能力及辐射特性。仿真结果表明,设计的天线具有低反射、宽频带、高增益和高耐压强度等优点。 展开更多
关键词 Valentine天线 巴伦 高功率脉冲 高耐压强度
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Soil
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作者 Valentine Ayamba Mbeng 《地学前缘》 EI CAS CSCD 北大核心 2009年第S1期237-237,共1页
Soil is one of the world’s most important resources.The scientific study of soil is known as pedology,ped stands for soil and logy means study.This is a whole science in itself which began in Russia in the early part... Soil is one of the world’s most important resources.The scientific study of soil is known as pedology,ped stands for soil and logy means study.This is a whole science in itself which began in Russia in the early part of the 20 century.Soil constitutes a major geographical factor known as Edaphic factor,which assists in the explanation of spatial patterns of phenomena on the earth’s surface including the spread of living things,which is the focus of biogeography.Soil is the thin surface layer resting on 展开更多
关键词 valentine ayamba SOIL
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Prevalence of Panton Valentine Leukocidin in Carriage and Infective Strains of <i>Staphylococcus aureus</i>at a Referral Hospital in Kenya
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作者 Geoffrey Omuse Patricia Shivachi +1 位作者 Samuel Kariuki Gunturu Revathi 《Open Journal of Medical Microbiology》 2013年第1期5-11,共7页
Panton valentine leukocidin (PVL) is a pore forming exotoxin that is expressed by some Staphylococcus aureus (S. aureus) strains and is thought to add to its virulence. The prevalence of PVL in carriage and disease ca... Panton valentine leukocidin (PVL) is a pore forming exotoxin that is expressed by some Staphylococcus aureus (S. aureus) strains and is thought to add to its virulence. The prevalence of PVL in carriage and disease causing strains varies considerably from region to region. This study compared the prevalence of the PVL gene in S. aureus isolates obtained from healthcare workers and from patients seen at the Aga Khan University Hospital Nairobi (AKUHN). S. aureus isolates obtained from healthcare workers and patients attended to at AKUHN between July 2010 and March 2011 were used for this study. Forty five S. aureus isolates from healthcare workers and 63 from clinical specimens obtained from 59 patients were analysed for the PVL gene. The prevalence of PVL in isolates from healthcare workers was 24.4% compared to 39.7% in the isolates causing infection (P = 0.098). PVL prevalence was 58.8% in S. aureus isolates obtained from skin and soft tissue infections (SSIs) compared to 25.0% in carriage isolates (P = 0.002, OR 4.29). Prevalence in isolates from invasive infections was 11.1%. Patients with PVL positive S. aureus were younger than those with PVL negative isolates (P = 0.082). The high prevalence of PVL is comparable with that reported in other African countries. The significance of the high prevalence of PVL in S.aureus isolates carried by health care workers at AKUHN is unclear at the moment. PVL prevalence is significantly higher in S. aureus isolates causing SSIs compared to carriage and invasive isolates. 展开更多
关键词 Staphylococcus AUREUS S. AUREUS Panton Valentine Leukocidin PVL
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Be My Valentine
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《疯狂英语(初中天地)》 北大核心 2006年第2期19-19,共1页
A man walks into a post office one day and sees a middle-aged, balding[秃头的] man standing at the
关键词 Be My Valentine
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五月的病毒捉出来
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作者 Kitten 《计算机》 2002年第22期30-30,共1页
1)W32/Magistr.B@mm病毒类型:蠕虫、多态可否修复:是在野病毒:是传播等级:高危险性:中病毒描述:这是一个蠕虫病毒,随操作系统不同各异。在Win9X下:用类似CIH的方法删除整个硬盘的数据,烧毁可擦写式BIOS。在WinNT和Win2000下:用“YOUARES... 1)W32/Magistr.B@mm病毒类型:蠕虫、多态可否修复:是在野病毒:是传播等级:高危险性:中病毒描述:这是一个蠕虫病毒,随操作系统不同各异。在Win9X下:用类似CIH的方法删除整个硬盘的数据,烧毁可擦写式BIOS。在WinNT和Win2000下:用“YOUARESHIT”字符串填充其找到的文件。感染此病毒后,它会向一些特定地址发一封已被感染的确认信,并且向此机器的通讯簿所记录的地址发送以自身为附件的信。 展开更多
关键词 计算机 病毒 Magistr.B@mm Concept.A 宏病毒 Valentin
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Happy Valentine’s Day!
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作者 Yuki 《美眉》 2019年第2期176-177,共2页
过了圣诞和新年,转眼就到了一年中最浪漫的节日—情人节.每年的情人节怎么少得了为男神亲手准备的甜点呢?俗话说'要想抓住一个人的心,就要先抓住他的胃',这次就跟着ViVi酱学一道可爱的情人节点心吧.
关键词 细砂糖 HAPPY Valentine’s DAY
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情人的礼物Valentine
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《中国体育(中英文版)》 2007年第3期108-109,共7页
情人节到了,是考验你经济实力和眼光的时刻来了……别以为情人节就是过了2月14。通常,2月14日是诉诸情意的日子,而在2月14日这天,如果一方在2月14收到礼物且对对方有同样好感时,就会在这天回送对方一份情人礼物,表示彼此已经心心相印,... 情人节到了,是考验你经济实力和眼光的时刻来了……别以为情人节就是过了2月14。通常,2月14日是诉诸情意的日子,而在2月14日这天,如果一方在2月14收到礼物且对对方有同样好感时,就会在这天回送对方一份情人礼物,表示彼此已经心心相印,这一天被人叫做"白色情人节"。嫌你在情人节送给他(她)的礼物不够独特,不够派?那就在3月14日好好补偿一下吧! 展开更多
关键词 白色情人节 Valentine 这一天 感时 你那 回送 等离子电视 向下兼容 二人世界 外力冲击
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我用KV杀病毒
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《电脑知识与技术(过刊)》 2004年第10期37-37,共1页
关键词 I-Worm/Sircam 网络蠕虫病毒 l-Worm/Valentin W97M/FF.b 宏病毒
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Valentine’s day,我拿什么感动你?
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《电子竞技》 2008年第3期114-117,共4页
当春天第一朵玫瑰盛开的时候,沉浸在爱河的人们都知道.他们将迎来属于自己的节日——情人节。当寄托着真挚爱意的鲜花、贺卡、巧克力献在情人面前时,一份能打动对方礼物也是必不可少的。于是花瓣凋零之后.你们的爱还有一个恒古不变的见... 当春天第一朵玫瑰盛开的时候,沉浸在爱河的人们都知道.他们将迎来属于自己的节日——情人节。当寄托着真挚爱意的鲜花、贺卡、巧克力献在情人面前时,一份能打动对方礼物也是必不可少的。于是花瓣凋零之后.你们的爱还有一个恒古不变的见证。如果你还在为今年的情人节礼物烦恼的话.希望这个专题能给你一些启发,我们也衷心祝愿天下所有有情人忠诚眷属。其实关于情人节的由来.有许多不同的典故,流传最广的应该属古罗马时的一个说法。当时有一个名叫Valentine的修士.他不忍看见暴君因战争需要而禁止男女青年婚恋的荒唐规定,主动为相爱男女主持婚礼.后来这位修士被打入大牢,折磨至死,他死的那一天是2月14日.人们为了纪念他,这一天就成为了今天的情人节,英语中的说法是Valentine's day。 展开更多
关键词 情人节 Valentine’s day
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My Forever Valentine
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作者 崔淑娴 《中学生英语(初中版)》 2008年第5期26-27,共2页
The traditional holidays in my family when I was a child were spent timing elaborate(精心制作的) meals around football games. My father tried to make pleasant chitchat(闲聊) and eat as much as he could during halftime... The traditional holidays in my family when I was a child were spent timing elaborate(精心制作的) meals around football games. My father tried to make pleasant chitchat(闲聊) and eat as much as he could during halftime. At Christmas he found time to have a cup or two of holiday cheer 展开更多
关键词 My Forever Valentine
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No Divorce on Chinese Valentine’s Day-Making Way for Love?
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《ChinAfrica》 2016年第10期12-13,共2页
The Qixi Festival, also known as Chinese Valentine's Day, has been celebrated as a symbo of eternal love in Chinese culture for thousands of years. Every year, many young Chinese couples decide to tie the knot on thi... The Qixi Festival, also known as Chinese Valentine's Day, has been celebrated as a symbo of eternal love in Chinese culture for thousands of years. Every year, many young Chinese couples decide to tie the knot on this day of romance, flooding local marriage registration. 展开更多
关键词 No Divorce on Chinese Valentine’s Day-Making Way for Love
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The character defining love in its many forms
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作者 Huang Weijia Chu Dandan 《The World of Chinese》 2025年第1期112-114,共3页
Valentine's Day is a day for lovers,and for non-lovers to wish never existed.In Chinese,the holiday translates to情人节(Qíngrén Jié),and not as you might guess,爱人节(àirén Jié),sinc... Valentine's Day is a day for lovers,and for non-lovers to wish never existed.In Chinese,the holiday translates to情人节(Qíngrén Jié),and not as you might guess,爱人节(àirén Jié),since爱人is exclusively used to refer to spouses.However,the character爱encompasses a world far larger than mere adoration and romance. 展开更多
关键词 LOVE valentines Day ADORATION Chinese ROMANCE valentines day
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耐甲氧西林金黄色葡萄球菌的耐药性及其Panton-Valentine杀白细胞素基因的检测 被引量:5
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作者 张征 孙静娜 +1 位作者 武艳 杨继章 《中国药房》 CAS CSCD 北大核心 2010年第2期127-129,共3页
目的:了解耐甲氧西林金黄色葡萄球菌(MRSA)对抗菌药物的耐药性及其Panton-Valentine杀白细胞素(PVL)基因的检出情况。方法:收集2007年1月~2008年6月分离自河北医科大学第一医院临床患者的MRSA45株,采用Kerby-Bauer纸片扩散法测定细菌... 目的:了解耐甲氧西林金黄色葡萄球菌(MRSA)对抗菌药物的耐药性及其Panton-Valentine杀白细胞素(PVL)基因的检出情况。方法:收集2007年1月~2008年6月分离自河北医科大学第一医院临床患者的MRSA45株,采用Kerby-Bauer纸片扩散法测定细菌对17种抗菌药物的敏感性;采用聚合酶链反应(PCR)技术检测PVL基因。结果:MRSA对青霉素G、红霉素、克林霉素、环丙沙星、四环素的耐药率均在70%以上,有12株MRSA检出PVL基因,检出率为26.7%。结论:MRSA呈多重耐药,PVL基因是MRSA的重要致病因子。 展开更多
关键词 耐甲氧西林金黄色葡萄球菌 Panton—Valentine杀白细胞素基因 耐药性
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A Valentine to One Who Cared-Too Much
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作者 NANCY J.RIGG 《中国翻译》 1981年第5期14-15,共2页
It’s raining, again. As I lie awake in bed, lis-tening to the sound of those razor-sharp dropspounding on the pavement, my mind goes reelingdown dark corridors teeming with agonizing flash-backs, and a chill from wit... It’s raining, again. As I lie awake in bed, lis-tening to the sound of those razor-sharp dropspounding on the pavement, my mind goes reelingdown dark corridors teeming with agonizing flash-backs, and a chill from within fills me with dread.It’s raining, again.It does this every year in Southern California;at least that’s what they told me last year whenI marveled at the relentless determination of therain. There seem to be two seasons here. Duringthe rainy season, sometimes the storms drench thearea nonstop for days. Sometimes the storms 展开更多
关键词 TH A Valentine to One Who Cared-Too Much
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Valentine’s Celebration at The Ritz-Carlton, Beijing
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《Beijing Review》 2015年第35期43-43,共1页
On this year’s Chinese Valentine’s Day which fell on August 20,we offered a romantic dining experience at our award-winning restaurants Aroma and Barolo from The Ritz-Carlton,Beijing.Endless Love at Aroma The amorou... On this year’s Chinese Valentine’s Day which fell on August 20,we offered a romantic dining experience at our award-winning restaurants Aroma and Barolo from The Ritz-Carlton,Beijing.Endless Love at Aroma The amorous ambiance and the succulent seafood buffet at Aroma,complemented by a screening of the movie Romeo and Juliet in Gazebo,al owed vips a memorable night of endless love. 展开更多
关键词 BEIJING Valentine’s Celebration at The Ritz-Carlton
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HOLIDAY SEASON Spring Festival is China's biggest celebration,but foreign holidays such as Christmas and Valentine's Day are making inroads
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作者 ZHANG ZHIPING 《Beijing Review》 2006年第4期20-23,1,共5页
As China opens to the world, Chinese people are embracing the Western world's holidays. Christmas, Valentine's Day and Halloween are all growing in popularity, as the commercial aspects of these celebrations f... As China opens to the world, Chinese people are embracing the Western world's holidays. Christmas, Valentine's Day and Halloween are all growing in popularity, as the commercial aspects of these celebrations find an audience. Young people say they feel increasingly detached from traditions associated with age-old Chinese festivals, such as Spring Festival, which starts January 29 this year and marks the lunar New Year. But Spring Festival isn't going anywhere yet, and this week the country heads home en mas... 展开更多
关键词 HOLIDAY SEASON Spring Festival is China’s biggest celebration but foreign holidays such as Christmas and Valentine’s Day are making inroads
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