从含有Vero毒素－1（VT1）全基因的基因工程菌中纯化出VT1，纯化的步骤包括（NH4）2SO4盐析，两次DEAE Sepharose Fast Folw柱层析，最终从4L培养物中纯化出1．5mg纯毒素，收率为8．6％，梯度N -PAGE测定毒素的分子量为70KDa,SDS-PAG...从含有Vero毒素－1（VT1）全基因的基因工程菌中纯化出VT1，纯化的步骤包括（NH4）2SO4盐析，两次DEAE Sepharose Fast Folw柱层析，最终从4L培养物中纯化出1．5mg纯毒素，收率为8．6％，梯度N -PAGE测定毒素的分子量为70KDa,SDS-PAGE电泳表明毒素为两种亚基，分子量分别是32KDa和7．7KDa，对VT1的多种生物学特性进行了研究，经测定VT1和Vero细胞的半数致死量CD50为1pg，对小鼠的半数致列量LD50为18ng，引起兔肠襻积液的最小毒素量为1．25ug/肠襻。展开更多
The rice blast fungus Magnaporthe oryzae stands as a formidable adversary to one of the world’s most important crops,rice,which feeds over half of the global population.Its ability to rapidly evolve and adapt undersc...The rice blast fungus Magnaporthe oryzae stands as a formidable adversary to one of the world’s most important crops,rice,which feeds over half of the global population.Its ability to rapidly evolve and adapt underscores the urgent need for a comprehensive understanding of its infection strategies.In a large-scale study published in Cell,Cruz-Mireles et al.(Cell 187:2557-73,2024)utilized phosphoproteomics to globally map the phosphorylation landscape during the infection-related development by M.oryzae,identifying 2062 activated phosphoproteins carrying 8005 phosphosites.A subset of these phosphosites were conserved in the proteins of diverse fungal pathogens and appeared to be associated with biotrophic and hemibiotrophic infection.Thirty-two of these phosphoproteins are regulated by pathogenicity mitogen-activated kinase 1(Pmk1),a central component of the MAPK signaling pathway,including VTi 1–2 suppressor,whose regulation by Pmk1 is essential for rice blast disease.Together,this global phosphorylation atlas offers a rich tapestry of potential therapeutic targets for developing green agrochemicals to control fungal diseases of plants.展开更多
文摘从含有Vero毒素－1（VT1）全基因的基因工程菌中纯化出VT1，纯化的步骤包括（NH4）2SO4盐析，两次DEAE Sepharose Fast Folw柱层析，最终从4L培养物中纯化出1．5mg纯毒素，收率为8．6％，梯度N -PAGE测定毒素的分子量为70KDa,SDS-PAGE电泳表明毒素为两种亚基，分子量分别是32KDa和7．7KDa，对VT1的多种生物学特性进行了研究，经测定VT1和Vero细胞的半数致死量CD50为1pg，对小鼠的半数致列量LD50为18ng，引起兔肠襻积液的最小毒素量为1．25ug/肠襻。
基金supported by the National Natural Science Foundation of China(32172363VB)+1 种基金the Chinese Universities Scientific Fund(10092004VB).
文摘The rice blast fungus Magnaporthe oryzae stands as a formidable adversary to one of the world’s most important crops,rice,which feeds over half of the global population.Its ability to rapidly evolve and adapt underscores the urgent need for a comprehensive understanding of its infection strategies.In a large-scale study published in Cell,Cruz-Mireles et al.(Cell 187:2557-73,2024)utilized phosphoproteomics to globally map the phosphorylation landscape during the infection-related development by M.oryzae,identifying 2062 activated phosphoproteins carrying 8005 phosphosites.A subset of these phosphosites were conserved in the proteins of diverse fungal pathogens and appeared to be associated with biotrophic and hemibiotrophic infection.Thirty-two of these phosphoproteins are regulated by pathogenicity mitogen-activated kinase 1(Pmk1),a central component of the MAPK signaling pathway,including VTi 1–2 suppressor,whose regulation by Pmk1 is essential for rice blast disease.Together,this global phosphorylation atlas offers a rich tapestry of potential therapeutic targets for developing green agrochemicals to control fungal diseases of plants.
