Epstein-Barr virus(EBV)infection is well-known for its association with lymphoproliferative disorders and various lymphomas,causing significant global morbidity and mortality.EBV-positive vitreoretinal lymphoma(VRL)is...Epstein-Barr virus(EBV)infection is well-known for its association with lymphoproliferative disorders and various lymphomas,causing significant global morbidity and mortality.EBV-positive vitreoretinal lymphoma(VRL)is exceedingly rare.As a result,the pathogenic role and genomic characteristics of EBV in VRL remain poorly understood.In this study,we employed droplet digital PCR(ddPCR)combined with EBV-specific immunofluorescence assay to detect EBV in the vitreous fluid of fifty-three VRL patients.We found that approximately 28%(15/53)of the patients were EBV positive.Analysis of clinical data showed that EBV-positive VRL patients had shorter progression-free survival(PFS)compared to EBV-negative patients(P=0.004).Additionally,through integration of EBV-targeted sequencing and PCR-based deep sequencing,we found that all five VRL-derived EBV genomes formed a distinct cluster within one phylogenetic branch.Meanwhile,several non-synonymous mutations were exclusively detected in the VRL group,including S229T in latent membrane protein 1(LMP1)and G2248R in the Epstein-Barr virus BamHI-PraL fragment 1(BPLF1).In conclusion,our findings suggest that EBV as a risk factor associated with poor prognosis in VRL,and we provide a genome-wide view of EBV sequence variations from VRL patients.This may offer insights into the pathogenic role of EBV in VRL and could potentially assist in the diagnosis and treatment of this disease.展开更多
腐蹄病是反刍动物绵羊、山羊、鹿和奶牛等常见的一种高度接触性传染病。节瘤拟杆菌是致病作用的主要病菌之一,它是通过IV型纤毛和细胞外蛋白酶而产生作用。但从腐蹄病发病症状看,节瘤拟杆菌所致疾病的严重程度不是相同的,据此节瘤拟杆...腐蹄病是反刍动物绵羊、山羊、鹿和奶牛等常见的一种高度接触性传染病。节瘤拟杆菌是致病作用的主要病菌之一,它是通过IV型纤毛和细胞外蛋白酶而产生作用。但从腐蹄病发病症状看,节瘤拟杆菌所致疾病的严重程度不是相同的,据此节瘤拟杆菌被分为毒性、弱毒性和良性菌,这种分类通过对该菌基因组毒性关联蛋白Vap(Virulence-associated protein)区域和毒性相关位点vrl(Virulence Related Locus)区域的研究,发现其致病特点与基因的顺序有很大联系。展开更多
基金supported by the Basic Research Youth Talent Development Program of Huashan Hospital,Fudan University(2024JC030).
文摘Epstein-Barr virus(EBV)infection is well-known for its association with lymphoproliferative disorders and various lymphomas,causing significant global morbidity and mortality.EBV-positive vitreoretinal lymphoma(VRL)is exceedingly rare.As a result,the pathogenic role and genomic characteristics of EBV in VRL remain poorly understood.In this study,we employed droplet digital PCR(ddPCR)combined with EBV-specific immunofluorescence assay to detect EBV in the vitreous fluid of fifty-three VRL patients.We found that approximately 28%(15/53)of the patients were EBV positive.Analysis of clinical data showed that EBV-positive VRL patients had shorter progression-free survival(PFS)compared to EBV-negative patients(P=0.004).Additionally,through integration of EBV-targeted sequencing and PCR-based deep sequencing,we found that all five VRL-derived EBV genomes formed a distinct cluster within one phylogenetic branch.Meanwhile,several non-synonymous mutations were exclusively detected in the VRL group,including S229T in latent membrane protein 1(LMP1)and G2248R in the Epstein-Barr virus BamHI-PraL fragment 1(BPLF1).In conclusion,our findings suggest that EBV as a risk factor associated with poor prognosis in VRL,and we provide a genome-wide view of EBV sequence variations from VRL patients.This may offer insights into the pathogenic role of EBV in VRL and could potentially assist in the diagnosis and treatment of this disease.
文摘腐蹄病是反刍动物绵羊、山羊、鹿和奶牛等常见的一种高度接触性传染病。节瘤拟杆菌是致病作用的主要病菌之一,它是通过IV型纤毛和细胞外蛋白酶而产生作用。但从腐蹄病发病症状看,节瘤拟杆菌所致疾病的严重程度不是相同的,据此节瘤拟杆菌被分为毒性、弱毒性和良性菌,这种分类通过对该菌基因组毒性关联蛋白Vap(Virulence-associated protein)区域和毒性相关位点vrl(Virulence Related Locus)区域的研究,发现其致病特点与基因的顺序有很大联系。
