BACKGROUND We have innovatively amalgamated membrane blood purification and centrifugal blood cell separation technologies to address the limitations of current artificial liver support(ALS)models,and develop a versat...BACKGROUND We have innovatively amalgamated membrane blood purification and centrifugal blood cell separation technologies to address the limitations of current artificial liver support(ALS)models,and develop a versatile plasma purification system(VPPS)through centrifugal plasma separation.AIM To investigate the influence of VPPS on long-term rehospitalization and mortality rates among patients with acute-on-chronic liver failure(ACLF).METHODS This real-world,prospective study recruited inpatients diagnosed with ACLF from the Second Xiangya Hospital of Central South University between October 2021 and March 2024.Patients were categorized into the VPPS and non-VPPS groups based on the distinct ALS models administered to them.Self-administered questionnaires,clinical records,and self-reported data served as the primary methods for data collection.The laboratory results were evaluated at six distinct time points.All patients were subjected to follow-up assessments for>12 months.Kaplan-Meier survival analyses and Cox proportional hazards models were used to evaluate the risks of hospitalization and mortality during the follow-up period.RESULTS A cohort of 502 patients diagnosed with ACLF was recruited,with 260 assigned to the VPPS group.On comparing baseline characteristics,the VPPS group exhibited a significantly shorter length of stay,higher incidence of spontaneous peritonitis and pulmonary aspergillosis compared to the non-VPPS group(P<0.05).Agehazard ratio(HR=1.142,95%CI:1.01-1.23,P=0.018),peritonitis(HR=2.825,95%CI:1.07-6.382,P=0.026),albumin(HR=0.67,95%CI:0.46-0.942,P=0.023),total bilirubin(HR=1.26,95%CI:1.01-3.25,P=0.021),international normalized ratio(HR=1.97,95%CI:1.21-2.908,P=0.014),and VPPS/non-VPPS(HR=3.24,95%CI:2.152-4.76,P<0.001)were identified as significant independent predictors of mortality in both univariate and multivariate analyses throughout the follow-up period.Kaplan-Meier survival analyses demonstrated significantly higher rehospitalization and mortality rates in the non-VPPS group compared to the VPPS group during follow-up of≥2 years(log-rank test,P<0.001).CONCLUSION These findings suggest that VPPS is safe and has a positive influence on prognostic outcomes in patients with ACLF.展开更多
With digital coding technology,reconfigurable intelligent surfaces(RISs)become powerful real-time sys-tems for manipulating electromagnetic(EM)waves.However,most automatic RIS designs involve exten-sive numerical simu...With digital coding technology,reconfigurable intelligent surfaces(RISs)become powerful real-time sys-tems for manipulating electromagnetic(EM)waves.However,most automatic RIS designs involve exten-sive numerical simulations of the unit,including the passive pattern and active devices,requiring high data acquisition and training costs.In addition,for passive patterns,the widely employed random pixe-lated method presents design efficiency and effectiveness challenges due to the massive pixel combina-tions and blocked excitation current flow in discrete patterns.To overcome these two critical problems,we propose a versatile RIS design paradigm with efficient topology representation and a separate design architecture.First,a non-uniform rational B-spline(NURBS)is introduced to represent continuous pat-terns and solve excitation current flow issues.This representation makes it possible to finely tune con-tinuous patterns with several control points,greatly reducing the pattern solution space by 20-fold and facilitating RIS optimization.Then,employing multiport network theory to separate the passive pat-tern and active device from the unit,the separate design architecture significantly reduces the dataset acquisition cost by 62.5%.Through multistep multiport calculation,the multistate EM responses of the RIS under different structural combinations can be quickly obtained with only one prediction of pattern response,thereby achieving dataset and model reuse for different RIS designs.With a hybrid continuous-discrete optimization algorithm,three examples—including two typical high-performance RISs and an ultra-wideband multilayer RIS—are provided to validate the superiority of our paradigm.Our work offers an efficient solution for RIS automatic design,and the resulting structure is expected to boost RIS appli-cations in the fields of wireless communication and sensing.展开更多
The recombinant suicide plasmid pYYvpr contained a Kan resistant cassette and a 778bp internal piece of the vpr gene. It was transformed into the wild type E.coli strain, MC 1061,which possessed the full-length vpr ge...The recombinant suicide plasmid pYYvpr contained a Kan resistant cassette and a 778bp internal piece of the vpr gene. It was transformed into the wild type E.coli strain, MC 1061,which possessed the full-length vpr gene and did not contain the λ pir gene. The recombinant suicide plasmid could not replicate in MC 1061. Colonies grew on the plates of Kan were analysed by PCR and Southern blot with two different Dig-probes,vpr and Kan R probes. One colony was detected vpr and Kan R gene by PCR and Southern blot showed the mutant had the different vpr map with the wild type. The isogenic knockout mutant, named MC1061 Δvpr,was confirmed.The mutant supported lysogenic infection of VT2 recombinant phage, but did not support lytic infection. All results showed that the vpr gene should be related to the lytic infection of VT2 phage.展开更多
基金Supported by Natural Science Foundation of Hunan Province,China,No.2022JJ30842 and No.2024JJ6560Clinical Medical Research Center for Viral Hepatitis of Hunan Province,No.2023SK4009Beijing iGandan Foundation,No.RGGJJ-2021-017 and No.iGandanF-1082022-RGG023.
文摘BACKGROUND We have innovatively amalgamated membrane blood purification and centrifugal blood cell separation technologies to address the limitations of current artificial liver support(ALS)models,and develop a versatile plasma purification system(VPPS)through centrifugal plasma separation.AIM To investigate the influence of VPPS on long-term rehospitalization and mortality rates among patients with acute-on-chronic liver failure(ACLF).METHODS This real-world,prospective study recruited inpatients diagnosed with ACLF from the Second Xiangya Hospital of Central South University between October 2021 and March 2024.Patients were categorized into the VPPS and non-VPPS groups based on the distinct ALS models administered to them.Self-administered questionnaires,clinical records,and self-reported data served as the primary methods for data collection.The laboratory results were evaluated at six distinct time points.All patients were subjected to follow-up assessments for>12 months.Kaplan-Meier survival analyses and Cox proportional hazards models were used to evaluate the risks of hospitalization and mortality during the follow-up period.RESULTS A cohort of 502 patients diagnosed with ACLF was recruited,with 260 assigned to the VPPS group.On comparing baseline characteristics,the VPPS group exhibited a significantly shorter length of stay,higher incidence of spontaneous peritonitis and pulmonary aspergillosis compared to the non-VPPS group(P<0.05).Agehazard ratio(HR=1.142,95%CI:1.01-1.23,P=0.018),peritonitis(HR=2.825,95%CI:1.07-6.382,P=0.026),albumin(HR=0.67,95%CI:0.46-0.942,P=0.023),total bilirubin(HR=1.26,95%CI:1.01-3.25,P=0.021),international normalized ratio(HR=1.97,95%CI:1.21-2.908,P=0.014),and VPPS/non-VPPS(HR=3.24,95%CI:2.152-4.76,P<0.001)were identified as significant independent predictors of mortality in both univariate and multivariate analyses throughout the follow-up period.Kaplan-Meier survival analyses demonstrated significantly higher rehospitalization and mortality rates in the non-VPPS group compared to the VPPS group during follow-up of≥2 years(log-rank test,P<0.001).CONCLUSION These findings suggest that VPPS is safe and has a positive influence on prognostic outcomes in patients with ACLF.
基金supported by the National Key Research and Development Program of China(2023YFB3811502)the National Science Foundation of China(62225108)+5 种基金the Fundamental Research Funds for the Central Universities(2242022k60003)the National Natural Science Foundation of China(62288101 and 62201139)the Jiangsu Province Frontier Leading Technology Basic Research Project(BK20212002)the Jiangsu Provincial Scientific Research Center of Applied Mathematics(BK20233002)the Fundamental Research Funds for the Central Universities(2242024RCB0005 and 2242024K30009)the 111 Project(111-2-05).
文摘With digital coding technology,reconfigurable intelligent surfaces(RISs)become powerful real-time sys-tems for manipulating electromagnetic(EM)waves.However,most automatic RIS designs involve exten-sive numerical simulations of the unit,including the passive pattern and active devices,requiring high data acquisition and training costs.In addition,for passive patterns,the widely employed random pixe-lated method presents design efficiency and effectiveness challenges due to the massive pixel combina-tions and blocked excitation current flow in discrete patterns.To overcome these two critical problems,we propose a versatile RIS design paradigm with efficient topology representation and a separate design architecture.First,a non-uniform rational B-spline(NURBS)is introduced to represent continuous pat-terns and solve excitation current flow issues.This representation makes it possible to finely tune con-tinuous patterns with several control points,greatly reducing the pattern solution space by 20-fold and facilitating RIS optimization.Then,employing multiport network theory to separate the passive pat-tern and active device from the unit,the separate design architecture significantly reduces the dataset acquisition cost by 62.5%.Through multistep multiport calculation,the multistate EM responses of the RIS under different structural combinations can be quickly obtained with only one prediction of pattern response,thereby achieving dataset and model reuse for different RIS designs.With a hybrid continuous-discrete optimization algorithm,three examples—including two typical high-performance RISs and an ultra-wideband multilayer RIS—are provided to validate the superiority of our paradigm.Our work offers an efficient solution for RIS automatic design,and the resulting structure is expected to boost RIS appli-cations in the fields of wireless communication and sensing.
文摘The recombinant suicide plasmid pYYvpr contained a Kan resistant cassette and a 778bp internal piece of the vpr gene. It was transformed into the wild type E.coli strain, MC 1061,which possessed the full-length vpr gene and did not contain the λ pir gene. The recombinant suicide plasmid could not replicate in MC 1061. Colonies grew on the plates of Kan were analysed by PCR and Southern blot with two different Dig-probes,vpr and Kan R probes. One colony was detected vpr and Kan R gene by PCR and Southern blot showed the mutant had the different vpr map with the wild type. The isogenic knockout mutant, named MC1061 Δvpr,was confirmed.The mutant supported lysogenic infection of VT2 recombinant phage, but did not support lytic infection. All results showed that the vpr gene should be related to the lytic infection of VT2 phage.
