[ Objective] To determine the best culture time and inducer for the biosynthesis of cellulase by Trichoderma vivide and thus provide the conditions for its practical application. [ Method] Within the 7 d after the ino...[ Objective] To determine the best culture time and inducer for the biosynthesis of cellulase by Trichoderma vivide and thus provide the conditions for its practical application. [ Method] Within the 7 d after the inoculation of Trichoderma vivide ZJ strain, the cultures were collected once every day, and the enzyme yield was respectively determined by 3,5-dinitresalicylic acid assay. The Trichodernm vivide ZJ strain was inoculated into basal medium added by different types of carbon sources or nitrogen sources, and the growth of Trichoderma viride was observed. And the mycalium weight as well as the yield of CMCase enzyme after different culture time was determined. [Result] The optimal culture time for Trichoderma viride ZJ strain was 72-96 h; it grew rapidly in the medium added by monosaccharide or disaccharide as carbon sources, and the production of CMCase enzyme reached a peak after 3 -4 d post inoculation. Cellulose powder was the best carbon inducer. The compound nitrogen source composed of 1 g/L ammonium sulfate and 2 g/L yeast extract was the most suitable for the growth of ZJ strain and produced the highest enzyme activity. [ Condusion] The largest enzyme yield should be obtained after 3-4 d post the inoculation of Trichoderma viride ZJ strain. With cellulose powder as a carbon source and the complex substance composed of ammonium sulfate and yeast extract as a nitrogen source, Trichoderma viride has the highest enzyme activity.展开更多
文摘[ Objective] To determine the best culture time and inducer for the biosynthesis of cellulase by Trichoderma vivide and thus provide the conditions for its practical application. [ Method] Within the 7 d after the inoculation of Trichoderma vivide ZJ strain, the cultures were collected once every day, and the enzyme yield was respectively determined by 3,5-dinitresalicylic acid assay. The Trichodernm vivide ZJ strain was inoculated into basal medium added by different types of carbon sources or nitrogen sources, and the growth of Trichoderma viride was observed. And the mycalium weight as well as the yield of CMCase enzyme after different culture time was determined. [Result] The optimal culture time for Trichoderma viride ZJ strain was 72-96 h; it grew rapidly in the medium added by monosaccharide or disaccharide as carbon sources, and the production of CMCase enzyme reached a peak after 3 -4 d post inoculation. Cellulose powder was the best carbon inducer. The compound nitrogen source composed of 1 g/L ammonium sulfate and 2 g/L yeast extract was the most suitable for the growth of ZJ strain and produced the highest enzyme activity. [ Condusion] The largest enzyme yield should be obtained after 3-4 d post the inoculation of Trichoderma viride ZJ strain. With cellulose powder as a carbon source and the complex substance composed of ammonium sulfate and yeast extract as a nitrogen source, Trichoderma viride has the highest enzyme activity.
