Mammalian macrophage migration inhibitory factor （MIF） plays an important role as an indispensable mediator in the pathogenesis of inflammatory disease like septicemia, but little is known about the role of MIF homo...Mammalian macrophage migration inhibitory factor （MIF） plays an important role as an indispensable mediator in the pathogenesis of inflammatory disease like septicemia, but little is known about the role of MIF homologue in fish septicemia. The authors have cloned the MIF homologue in large yellow croaker Pseudosciaena crocea （LycMIF） using RACE approach. The full-length cDNA of LycMIF was 634 bases and contained an ORF of 345 bases encoding a protein of 115 amino acid residues. As demonstrated by RT-PCR and QRT-PCR assay, MIF mRNAs were constitutively expressed in 11 selected tissues and were abundant in brain and liver. Moreover, the LycMIF transcripts in the liver and head kidney were responsive to bacteria infection and could be significantly up-regulated. Our results provide the first direct evidence that fish MIF was implicated in pathogenesis of fish vibrosis and play an important role in response to bacteria infection.展开更多
The pathogenic species of genus Vibrio cause vibriosis, one of the most prevalent diseases of maricultured animals and seafood consumers. Monitoring their kinetics in the chain of seafood production, processing and co...The pathogenic species of genus Vibrio cause vibriosis, one of the most prevalent diseases of maricultured animals and seafood consumers. Monitoring their kinetics in the chain of seafood production, processing and consumption is of great importance for food and mariculture safety. In order to enrich Vibrio-representing 16S ribosomal RNA gene （rDNA） fragments and identify these bacteria further real-timely and synchronously among bacterial flora in the chain, a pair of primers that selectively amplify Vibrio 16S rDNA fragments were designed with their specificities and coverage testified in the analysis of seawater Vibrio community. The specificities and coverage of two primers, VF169 and VR744, were determined theoretically among bacterial 16S rDNAs available in GenBank by using BLAST program and practically by amplifying Vibrio 16S rDNA fragments from seawater DNA. More than 88.3% of sequences in GenBank, which showed identical matches with VR744, belong to Vibrio genus. A total of 33 clones were randomly selected and sequenced. All of the sequences showed their highest similarities to and clustered around those of diverse known Vibrio species. The primers designed are capable of retrieving a wide range of Vibrio 16S rDNA fragments specifically among bacterial flora in seawater, the most important natural environment of seafood cultivation.展开更多
Bacteria of the genus Vibrio are ubiquitously distributed in the marine environment. Due to the rapid expansion of intensive mariculture and the consequent deterioration of culture conditions, more and more Vibrio spp...Bacteria of the genus Vibrio are ubiquitously distributed in the marine environment. Due to the rapid expansion of intensive mariculture and the consequent deterioration of culture conditions, more and more Vibrio spp. have been recognized as pathogenic agents in outbreaks of vibriosis, a serious epizootic disease affecting most wild and farmed fish species worldwide, which has become the most important limiting factor for the development of intensive mariculture industry. Attempts have been made to understand the pathogenicity of vibrios in host fish with the ultimate aim of elucidating the best means for disease control. After an extensive literature survey of the recent advances in the field of fish vibriosis, the pathological changes, virulence factors and associated potential pathogenic mechanisms, transmission routes and related environmental factors involved in outbreak of vibriosis, as well as the controlling strategies are reviewed in the present paper.展开更多
The bacteria in the genus Vibrio are heterothrophic, which exist in the larval rearing water of Crustacea and often show diverse pathogenicities to marine animals. In order to assess the bacterial diversity associated...The bacteria in the genus Vibrio are heterothrophic, which exist in the larval rearing water of Crustacea and often show diverse pathogenicities to marine animals. In order to assess the bacterial diversity associated with Crustacean seed production, 32 strains were isolated from black tiger shrimp (Penaeus monodon) and mangrove crab (Scylla paramamosain) larvae and their rearing-water and characterized using biochemical and molecular approaches. Two or more genotypically different species were identified. The vibriosis of black tiger shrimp was caused by V. harveyi, V. alginolyticus and Vibrio spp. predominantly, while that of crab by V. harveyi and V. alginolyticus only.展开更多
Vibrionaceae are a common bacterial disease that affects both wild and farmed marine fishes and causes tremendous economic losses globally.In order to investigate the prevalence,molecular typing,antibiogram and pathog...Vibrionaceae are a common bacterial disease that affects both wild and farmed marine fishes and causes tremendous economic losses globally.In order to investigate the prevalence,molecular typing,antibiogram and pathogenicity of vibriosis among cultured sea bream,two hundred and fifty Gilthead seabream(Sparus aurata)were collected randomly from different mariculture farms at Ismailia and Port Said Governorates.The collected fish were subjected to clinical,postmortem,bacteriological,and histopathological examinations.The majority of infected fish displayed ascites,hemorrhagic protruded anus,hemorrhages in pectoral,dorsal and tail fins,rotten gills,thinning of the head,discoloration of the skin,besides ulcer in the mouth and on the skin.The prevalence of Vibrio infection mostly was noticed at summer(35%)and spring(26%)then(22.5%)in autumn,where the kidney was the most predominant affected organ(45%).Vibrio 16s rRNA gene PCR extension generated a 663 pb amplified DNA bands that characteristic for all tested vibrio isolates.Blasting identified as V.harveyi and the other V.parahaemolyticus.The histopathological examination of naturally infected Gilthead seabream exhibited mild to moderate vacuolar degeneration of the hepatic parenchyma with tubular-nephrosis and massive renal cellular destruction.The splenic tissues showed focal activated melanomacrophage centers.The antimicrobial sensitivity was carried out,where the recovered strains were completely sensitive to novobiocin and highly resistant to ampicillin.In conclusion,the synergism of phenotypic and genotypic characterization is a valuable epidemiological tool for the diagnosis of Vibrio species.Strict veterinary hygienic regulations should be imple-mented to control such infections and minimize the antimicrobial use in fish farms.展开更多
基金The National Natural Science Foundation of China under contract No. 40976096the National Department Public Benefit Research Foundation of China under contract No. 200903029the National High Technology Research and Development Program of China (863 Program) under contract No. 2006AA10A405
文摘Mammalian macrophage migration inhibitory factor （MIF） plays an important role as an indispensable mediator in the pathogenesis of inflammatory disease like septicemia, but little is known about the role of MIF homologue in fish septicemia. The authors have cloned the MIF homologue in large yellow croaker Pseudosciaena crocea （LycMIF） using RACE approach. The full-length cDNA of LycMIF was 634 bases and contained an ORF of 345 bases encoding a protein of 115 amino acid residues. As demonstrated by RT-PCR and QRT-PCR assay, MIF mRNAs were constitutively expressed in 11 selected tissues and were abundant in brain and liver. Moreover, the LycMIF transcripts in the liver and head kidney were responsive to bacteria infection and could be significantly up-regulated. Our results provide the first direct evidence that fish MIF was implicated in pathogenesis of fish vibrosis and play an important role in response to bacteria infection.
基金This study was supported by grants from the International Found for Sciences(No.A/3224-1)Cooperative Project of the Key Lab of Freshwater Germplasm and Biotechnology of Chinese Ministry of Agriculture(No.LFB20040503)+1 种基金the National Natural Science Foundation of China(No.40176028)the National Key R&D Program(‘973'Program)of China(No.G1999012005).
文摘The pathogenic species of genus Vibrio cause vibriosis, one of the most prevalent diseases of maricultured animals and seafood consumers. Monitoring their kinetics in the chain of seafood production, processing and consumption is of great importance for food and mariculture safety. In order to enrich Vibrio-representing 16S ribosomal RNA gene （rDNA） fragments and identify these bacteria further real-timely and synchronously among bacterial flora in the chain, a pair of primers that selectively amplify Vibrio 16S rDNA fragments were designed with their specificities and coverage testified in the analysis of seawater Vibrio community. The specificities and coverage of two primers, VF169 and VR744, were determined theoretically among bacterial 16S rDNAs available in GenBank by using BLAST program and practically by amplifying Vibrio 16S rDNA fragments from seawater DNA. More than 88.3% of sequences in GenBank, which showed identical matches with VR744, belong to Vibrio genus. A total of 33 clones were randomly selected and sequenced. All of the sequences showed their highest similarities to and clustered around those of diverse known Vibrio species. The primers designed are capable of retrieving a wide range of Vibrio 16S rDNA fragments specifically among bacterial flora in seawater, the most important natural environment of seafood cultivation.
文摘Bacteria of the genus Vibrio are ubiquitously distributed in the marine environment. Due to the rapid expansion of intensive mariculture and the consequent deterioration of culture conditions, more and more Vibrio spp. have been recognized as pathogenic agents in outbreaks of vibriosis, a serious epizootic disease affecting most wild and farmed fish species worldwide, which has become the most important limiting factor for the development of intensive mariculture industry. Attempts have been made to understand the pathogenicity of vibrios in host fish with the ultimate aim of elucidating the best means for disease control. After an extensive literature survey of the recent advances in the field of fish vibriosis, the pathological changes, virulence factors and associated potential pathogenic mechanisms, transmission routes and related environmental factors involved in outbreak of vibriosis, as well as the controlling strategies are reviewed in the present paper.
文摘The bacteria in the genus Vibrio are heterothrophic, which exist in the larval rearing water of Crustacea and often show diverse pathogenicities to marine animals. In order to assess the bacterial diversity associated with Crustacean seed production, 32 strains were isolated from black tiger shrimp (Penaeus monodon) and mangrove crab (Scylla paramamosain) larvae and their rearing-water and characterized using biochemical and molecular approaches. Two or more genotypically different species were identified. The vibriosis of black tiger shrimp was caused by V. harveyi, V. alginolyticus and Vibrio spp. predominantly, while that of crab by V. harveyi and V. alginolyticus only.
文摘Vibrionaceae are a common bacterial disease that affects both wild and farmed marine fishes and causes tremendous economic losses globally.In order to investigate the prevalence,molecular typing,antibiogram and pathogenicity of vibriosis among cultured sea bream,two hundred and fifty Gilthead seabream(Sparus aurata)were collected randomly from different mariculture farms at Ismailia and Port Said Governorates.The collected fish were subjected to clinical,postmortem,bacteriological,and histopathological examinations.The majority of infected fish displayed ascites,hemorrhagic protruded anus,hemorrhages in pectoral,dorsal and tail fins,rotten gills,thinning of the head,discoloration of the skin,besides ulcer in the mouth and on the skin.The prevalence of Vibrio infection mostly was noticed at summer(35%)and spring(26%)then(22.5%)in autumn,where the kidney was the most predominant affected organ(45%).Vibrio 16s rRNA gene PCR extension generated a 663 pb amplified DNA bands that characteristic for all tested vibrio isolates.Blasting identified as V.harveyi and the other V.parahaemolyticus.The histopathological examination of naturally infected Gilthead seabream exhibited mild to moderate vacuolar degeneration of the hepatic parenchyma with tubular-nephrosis and massive renal cellular destruction.The splenic tissues showed focal activated melanomacrophage centers.The antimicrobial sensitivity was carried out,where the recovered strains were completely sensitive to novobiocin and highly resistant to ampicillin.In conclusion,the synergism of phenotypic and genotypic characterization is a valuable epidemiological tool for the diagnosis of Vibrio species.Strict veterinary hygienic regulations should be imple-mented to control such infections and minimize the antimicrobial use in fish farms.
