Transcription factors(TFs)play critical roles in the control of development and pathogenicity of phytopathogens by directly or indirectly regulating the expression of downstream genes.Here,we identified and characteri...Transcription factors(TFs)play critical roles in the control of development and pathogenicity of phytopathogens by directly or indirectly regulating the expression of downstream genes.Here,we identified and characterized a zinc finger TF UvMsn2 in Ustilaginoidea virens,a homolog of MoMsn2 from the rice blast fungus.Heterogenous complementation assay revealed that UvMsn2 fully restored the defects of theΔMomsn2 mutant in vegetative growth,conidiation and pathogenicity.Deletion of UvMsn2 in U.virens led to a reduction of the pathogen in vegetative growth,aerial hyphae and conidiation.Additionally,theΔUvmsn2 mutant displayed defects in conidial morphology and germination,as well as mitochondrial morphology.Pathogenicity and toxicity assays revealed that theΔUvmsn2 mutant was non-pathogenic and less inhibitory to rice seed germination.TheΔUvmsn2 mutant showed different sensitivity to various stresses.Further microscopic examination found that UvMsn2 was localized in both cytosol and nucleus,and translocated from cytosol to nucleus under the treatment of NaCl.Our results demonstrate that UvMsn2 is a critical TF that regulates the vegetative growth,conidiogenesis,stress response,mitochondrial morphology and virulence in the rice false smut fungus.展开更多
基金supported by the National Key Research and Development Program of China(Grant No:2016YFD0300700/2016YFD0300706)the Natural Science Foundation of China(Grant No:32061143045 to H.Zhang)the Fundamental Research Funds for the Central Universities(Grant No:KYYZ201903 to H.Zhang).
文摘Transcription factors(TFs)play critical roles in the control of development and pathogenicity of phytopathogens by directly or indirectly regulating the expression of downstream genes.Here,we identified and characterized a zinc finger TF UvMsn2 in Ustilaginoidea virens,a homolog of MoMsn2 from the rice blast fungus.Heterogenous complementation assay revealed that UvMsn2 fully restored the defects of theΔMomsn2 mutant in vegetative growth,conidiation and pathogenicity.Deletion of UvMsn2 in U.virens led to a reduction of the pathogen in vegetative growth,aerial hyphae and conidiation.Additionally,theΔUvmsn2 mutant displayed defects in conidial morphology and germination,as well as mitochondrial morphology.Pathogenicity and toxicity assays revealed that theΔUvmsn2 mutant was non-pathogenic and less inhibitory to rice seed germination.TheΔUvmsn2 mutant showed different sensitivity to various stresses.Further microscopic examination found that UvMsn2 was localized in both cytosol and nucleus,and translocated from cytosol to nucleus under the treatment of NaCl.Our results demonstrate that UvMsn2 is a critical TF that regulates the vegetative growth,conidiogenesis,stress response,mitochondrial morphology and virulence in the rice false smut fungus.
