Autophagy is an evolutionarily conserved degradation process that degrades damaged proteins to maintain homeostasis and to protect cells against stress.In this study,we identified and characterized a critical autophag...Autophagy is an evolutionarily conserved degradation process that degrades damaged proteins to maintain homeostasis and to protect cells against stress.In this study,we identified and characterized a critical autophagy-related protein,UvAtg14,in Ustilaginoidea virens,which is the ortholog of MoAtg14 in rice blast fungus Magnaporthe oryzea.UvAtg14 is co-localized with UvAtg8(an autophagy marker protein)and is highly expressed at 1-3 days post-inoculation.Deletion of the UvATG14 gene blocked GFP-UvAtg8 trafficking and autophagic digestion and significantly reduced mycelial growth,asexual reproduction,and virulence of U.virens.UvATG14 deletion mutants also exhibited increased sensitivity to various abiotic stresses.Our findings indicate that UvAtg14 is a key autophagic protein and contributes to mycelial growth,conidia production,and pathogenicity in U.virens.展开更多
基金supported by National Natural Science Foundation of China(31301624 and 31571961)Jiangsu Agricultural Science and Technology Innovation Fund(CX(20)3124).
文摘Autophagy is an evolutionarily conserved degradation process that degrades damaged proteins to maintain homeostasis and to protect cells against stress.In this study,we identified and characterized a critical autophagy-related protein,UvAtg14,in Ustilaginoidea virens,which is the ortholog of MoAtg14 in rice blast fungus Magnaporthe oryzea.UvAtg14 is co-localized with UvAtg8(an autophagy marker protein)and is highly expressed at 1-3 days post-inoculation.Deletion of the UvATG14 gene blocked GFP-UvAtg8 trafficking and autophagic digestion and significantly reduced mycelial growth,asexual reproduction,and virulence of U.virens.UvATG14 deletion mutants also exhibited increased sensitivity to various abiotic stresses.Our findings indicate that UvAtg14 is a key autophagic protein and contributes to mycelial growth,conidia production,and pathogenicity in U.virens.
