Objective:To investigate the regulatory effects of two traditional mineral medicines(TMMs),Gypsum Fibrosum(Shigao,GF)and Terra Flava Usta(Zaoxintu,TFU),on gut-beneficial bacteria in mice,and preliminarily explore thei...Objective:To investigate the regulatory effects of two traditional mineral medicines(TMMs),Gypsum Fibrosum(Shigao,GF)and Terra Flava Usta(Zaoxintu,TFU),on gut-beneficial bacteria in mice,and preliminarily explore their mechanisms of action.Methods:Mice were randomly divided into 3 groups(n=10 per group):the control group(standard diet),the GF group(diet supplemented with 2%GF),and the TFU group(diet supplemented with 2%TFU).After 4-week intervention,16S rRNA gene sequencing was used to analyze the changes in the gut microbiota(GM).Scanning electron microscopy,in combination with coumarin A tetramethyl rhodamine conjugate and Hoechst stainings,was used to observe the bacteria and biofilm formation.Results:Principal coordinate analysis revealed that GF and TFU significantly altered the GM composition in mice.Further analysis revealed that GF and TFU affected different types of gut bacteria,suggesting that different TMMs may selectively modulate specific bacterial populations.For certain bacteria,such as Faecalibaculum and lleibacterium,both GF and TFU exhibited growth-promoting effects,implying that they may be sensitive to TMMs and that different TMMs can increase their abundance through their respective mechanisms.Notably,Lactobacillus reuteri,a widely recognized and used probiotic,was significantly enriched in the GF group.Random forest analysis identified lleibacterium valens as a potential indicator bacterium for TMMs'impact on GM.Further mechanistic studies showed that gut bacteria formed biofilm structures on the TFU surface.Conclusions:This study provides new insights into the interaction between TMMs and GM.As safe and effective natural clays,GF and TFU hold promise as potential candidates for prebiotic development.展开更多
目的:通过比较灶心土、赤石脂和禹余粮的热解特性及红外光谱相似度来探讨赤石脂、禹余粮替代灶心土的可行性。方法:热解特性研究采用热重差热综合热分析仪,取试样量(30±5)mg置于坩锅中,以10℃·min^(-1)的升温速率、流量60 m L...目的:通过比较灶心土、赤石脂和禹余粮的热解特性及红外光谱相似度来探讨赤石脂、禹余粮替代灶心土的可行性。方法:热解特性研究采用热重差热综合热分析仪,取试样量(30±5)mg置于坩锅中,以10℃·min^(-1)的升温速率、流量60 m L·min^(-1)的恒定流速通入模拟空气[N_2-O_2(4∶1)],从室温升至900℃。红外光谱试验采用溴化钾压片法,光谱分辨率4 cm^(-1),测量范围4 000~500 cm-1,扫描信号累加32次,所得数据经Omnic软件进行自动基线校正、平滑及纵坐标归一化。数据处理及图表绘制采用Origin 8.0软件和Omnic软件完成。结果:比较热解特性发现灶心土、赤石脂、禹余粮的热解特性均在400~600℃处出现一段失重台阶;但整体而言,赤石脂与禹余粮热解特性没有体现出灶心土热解特性的复杂性;通过红外光谱相似度数值得出,以灶心土作为对照,赤石脂与灶心土相似度数值较偏低,禹余粮与与灶心土相似度数值处于中等水平。结论:就热解特性及红外光谱相似度而言,禹余粮与灶心土相似性较好,可能较赤石脂更适用作灶心土的替代品,为相关中药炮制辅料的研究提供参考。展开更多
基金Supported by Natural Science Foundation of China(No.31770540)the Key Research Program of Jiangsu Province(No.BE2018663)。
文摘Objective:To investigate the regulatory effects of two traditional mineral medicines(TMMs),Gypsum Fibrosum(Shigao,GF)and Terra Flava Usta(Zaoxintu,TFU),on gut-beneficial bacteria in mice,and preliminarily explore their mechanisms of action.Methods:Mice were randomly divided into 3 groups(n=10 per group):the control group(standard diet),the GF group(diet supplemented with 2%GF),and the TFU group(diet supplemented with 2%TFU).After 4-week intervention,16S rRNA gene sequencing was used to analyze the changes in the gut microbiota(GM).Scanning electron microscopy,in combination with coumarin A tetramethyl rhodamine conjugate and Hoechst stainings,was used to observe the bacteria and biofilm formation.Results:Principal coordinate analysis revealed that GF and TFU significantly altered the GM composition in mice.Further analysis revealed that GF and TFU affected different types of gut bacteria,suggesting that different TMMs may selectively modulate specific bacterial populations.For certain bacteria,such as Faecalibaculum and lleibacterium,both GF and TFU exhibited growth-promoting effects,implying that they may be sensitive to TMMs and that different TMMs can increase their abundance through their respective mechanisms.Notably,Lactobacillus reuteri,a widely recognized and used probiotic,was significantly enriched in the GF group.Random forest analysis identified lleibacterium valens as a potential indicator bacterium for TMMs'impact on GM.Further mechanistic studies showed that gut bacteria formed biofilm structures on the TFU surface.Conclusions:This study provides new insights into the interaction between TMMs and GM.As safe and effective natural clays,GF and TFU hold promise as potential candidates for prebiotic development.
