In this study, five hundred urine samples and five hundred blood samples of abusers were examined for the presence of alkaloid substances and abuse drugs in urines and bloods. These numbers of blood and urine samples ...In this study, five hundred urine samples and five hundred blood samples of abusers were examined for the presence of alkaloid substances and abuse drugs in urines and bloods. These numbers of blood and urine samples of addicts in clinics of welfare organization, during detoxification treatment or maintenance treatment were screened for abuse drugs presence. The all of samples were tested through as a view of clinical laboratory methods. Age ranges of female patients were 35 ~ 15 and age range of males patients were 45 ~ 15. All patients filled questionnaire and satisfy forms too. First, all fresh urine and blood samples were examined to confirm presence drugs abuses, depend on their addiction and treatment, so all samples were confirmed by two tests. Then they were examined to other clinical laboratory tests. All data were analyzed by t-test and were Anova one way and two ways of Anova Turkey, and p 〈 0.05 was considered significant. The p-value of this study was p = 0.0001. The results of this study were showed that 4% of abusers had mild increase in hematocrite level and 2% of narcotic drugs abusers had mild lower level of blood sugars than normal range and 4% of participants had increase liver enzymes such ALT （alanine transferase）, AST （aspartat transferase）, ALP （alkaline phosphatease） and 1% of them had renal failure. Although blood level BUN （blood urea nitrogen） and creatinin were examined to evaluation of their renal failure .The results in Tabriz/Iran undrevision of welfare organization clinics were approximately showed that positive results of addiction are in each of urine and blood samples. Because some of abusers directly consumed full long time agonist or partial agonists＇ drugs such as methadone and buprenorphine for their maintenance therapy in clinics. Also doing test on blood samples has high importance in distinguishing and confirmation of drugs abuse in samples. Also in this study we conclude that among all drug analytical methods the cheapest and easiest test to screening opioids and other abuse drugs in urine and blood samples is strip test for rapid diagnosis, also tests on blood samples have high importance as a view point of accuracy to distinguishing of drugs abuse, and serum levels of some other parameters showed all abusers patients situation such as liver and renal dysfimction through clinical laboratory tests.展开更多
Objective:To establish a rapid analysis method for the activity concentration of carbon-14(14C)in urine,in order to estimate the internal dose of 14C exposure,and to protect the health of occupational population.Metho...Objective:To establish a rapid analysis method for the activity concentration of carbon-14(14C)in urine,in order to estimate the internal dose of 14C exposure,and to protect the health of occupational population.Methods:Liquid scintillation counting(LSC)combined with the function of transformed spectral index of the external standard spectrum(tSIE)was used to measure the quenching level and counting rate,and the spiked urine samples with different shades of color were measured by LSC.After establishing the efficiency-quenching curve,the quenching correction and activity concentration analysis of the actual samples were carried out.Results:By LSC and the data fitting,the relationship between efficiency and quenching index could be represented using the equation y=0.0013x-0.0177(R^(2)=0.978).Three actual spiked samples were tested to verify this method,with recoveries of 97%,102%,and 89%,respectively.14C activity concentration of 4 actual urine samples were 0.12,0.11,0.10 and 0.08 Bq/mL,respectively,while the corresponding extended relative uncertainties were 0.0652,0.0929,0.0893 and 0.1043,respectively.Conclusion:The direct analysis method of 14C activity concentration in urine samples was established using LSC.The recovery of 14C activity concentration in urine samples showed that the proposed method had relatively high accuracy.By studying on the source of uncertainty,the uncertainty of the analysis results mainly came from the statistical error of LSC,and the uncertainty component of counting efficiency.展开更多
In this work,the application of silver nanoparticles(Ag NPs)for spectrophotometric determination of cefdinir(CFD)and cefepime HCl(CFP)was developed.The method was based on the ability of the alkaline degradation produ...In this work,the application of silver nanoparticles(Ag NPs)for spectrophotometric determination of cefdinir(CFD)and cefepime HCl(CFP)was developed.The method was based on the ability of the alkaline degradation products of the cited drugs to reduce Ag+ions to silver nanoparticles(Ag-NPs)in the presence of gelatin as a capping agent.The linear concentration ranges were 5.0-40 and 10-100μg/mL for cefdinir and cefepime HCl,respectively.The lower detection limits were found to be 0.39 and 1.06μg/mL for CFD and CFP,respectively,while the quantitation limits were found to be 1.18 and 3.21μg/mL for CFD and CFP,respectively.The formed nanoparticles were characterized by ultraviolet-visible spectroscopy and transmission electron microscopy.The proposed method was applied successfully to determination of CFD and CFP in pure drugs,pharmaceutical preparations and human urine samples.展开更多
In this study, one hundred urine samples and one hundred blood samples of abusers were examined for the presence of alkaloid substances and abuse drugs in urines and bloods. These numbers of blood and urine samples re...In this study, one hundred urine samples and one hundred blood samples of abusers were examined for the presence of alkaloid substances and abuse drugs in urines and bloods. These numbers of blood and urine samples referred who addicts in clinics of Welfare Organization, during of detoxification treatment or maintenance treatment were screened for abuse drugs presence. Age ranges of female patients were 35 ± 15 and age range of males patients were 42 ± 18. All patients filled questionnaire and satisfy forms too. All data were analyzed by t-test and were Anowa one way, and P 〈 0.05 was considered significant. The P value of this study was P = 0.000. In this study we conclude that among all drug analytical methods the cheapest and easiest test to screening opioids and other abuse drugs in urine and blood samples is strip test for rapid diagnosis and TLC （thin-layer chromatography） is appropriate confirmation method to drug abuse distinguishing. Also tests on blood samples have high importance as a view point of accuracy to distinguishing of drugs abuse.展开更多
A new method was developed for pre-concentration and determination of multiple drugs of abuse in human urine using dispersive liquid–liquid microextraction(DLLME)and capillary electrophoresis(CE)with photodiode array...A new method was developed for pre-concentration and determination of multiple drugs of abuse in human urine using dispersive liquid–liquid microextraction(DLLME)and capillary electrophoresis(CE)with photodiode array detection.The method was based on the formation of tiny droplets of an organic extractant in the prepared sample solution using water-immiscible organic solvent(chloroform)dissolved in water-miscible organic dispersive solvent(isopropyl alcohol).The organic phase,which extracted eight drugs of abuse from the prepared urine solution,was separated by centrifugation.The sedimented phase was transferred into a small volume CE auto-sampler vial with 10μL of 1%HCl methanol solution and evaporated to dryness.The residue was reconstituted in lidocaine hydrochloride(internal standard)aqueous solution and introduced by electrokinetic injection into CE.Under the optimum conditions,acceptable linear relationship was observed in the range of 3.0–500 ng/mL with the correlation coefficient(r)of 0.9982–0.9994 for spiked urine samples.The limit of detection(LOD)(S/N=3)was estimated to be 1.0 ng/mL.A recovery of 75.7%–90.6%was obtained for spiked samples.The mean relative error(MRE)was within±7.0%and the relative standard deviation(RSD)was less than 6.9%.The proposed DLLME-CE procedure offers an alternative analytical approach for the sensitive detection of drugs of abuse in real urine samples.展开更多
Introduction:On March 20,2025,a suspected Zika virus(ZIKV)case departed Thailand and flew to Nanning Wuxu International Airport before transiting to Jinan.Upon receiving notification,local CDCs immediately initiated c...Introduction:On March 20,2025,a suspected Zika virus(ZIKV)case departed Thailand and flew to Nanning Wuxu International Airport before transiting to Jinan.Upon receiving notification,local CDCs immediately initiated comprehensive epidemiological investigations,laboratory testing,and preventive control measures.Methods:We collected urine,sputum,and blood samples from the patient for analysis.Quantitative real-time reverse-transcription polymerase chain reaction(qRT-PCR)was employed to detect ZIKV nucleic acid.Metagenome Next-Generation Sequencing(mNGS)was performed on the urine sample to obtain complete viral genome sequences.Phylogenetic analysis was subsequently constructed using the obtained sequences to determine the origin,genotype,and mutation profile of this imported case.Results:The qRT-PCR analysis confirmed ZIKV presence in the patient’s urine,sputum,and serum samples.The mNGS successfully generated the complete ZIKV genome sequence.Phylogenetic analysis demonstrated that the ZIKV strain belonged to the Asian lineage,exhibiting 99.57%nucleotide homology with a ZIKV strain from Bangkok,Thailand(GenBank accession no.OR264645.1).Conclusion:Based on the patient’s epidemiological history,clinical presentation,and nucleic acid test results from multiple specimens,this case was confirmed as the first imported ZIKV infection documented in Shandong Province,with the infection source traced to Thailand.展开更多
Bisphenol A(BPA)and its bisphenol analogs(BPs)serve as key precursors in the manufacturing of polycarbonate plastics and epoxy resins.However,due to BPA’s detrimental health effects,there has been a growing global co...Bisphenol A(BPA)and its bisphenol analogs(BPs)serve as key precursors in the manufacturing of polycarbonate plastics and epoxy resins.However,due to BPA’s detrimental health effects,there has been a growing global consensus to restrict its use.The elderly,being particularly susceptible to neurodegenerative disorders,represent a population of concern;yet,research exploring the association between BPs exposure and neurodegenerative diseases in the elderly remains scarce.In this study,the urinary levels of six BPs among 267 participants from the Shenzhen aging-related disorder cohort in China during 2017–2018 were measured.Cognitive status was evaluated using the Mini-Mental State Examination(MMSE),and the impact of sociodemographic factors,lifestyle habits,and dietary practices was analyzed through the Extreme Gradient Boosting(XGBoost)classifier.In this study,BPA was the highest urinary level of bisphenol compound detected in the high-risk group(2.10 ng/mL,95.2%).Significant differences in BPs levels were observed to the degree of education,tea consumption frequency,meat intake frequency,and distance of main road groups(P<0.05).Notably,the absence of a significant positive correlation between BPF and BPA levels in high-risk groups for cognitive impairment implies more findings may be needed to fully evaluate BPF’s actual human exposure levels.The XGBoost models have further revealed that BPA analogs exposure and certain housing characteristics were substantial contributors to the potential impact on cognitive impairment during aging not only BPA.展开更多
Monitoring telomerase activity with high sensitive and reliable is of great importance to cancer analysis. In this paper, we report a sensitive and facile method to detect telomerase activity using AIEgens mod- ified ...Monitoring telomerase activity with high sensitive and reliable is of great importance to cancer analysis. In this paper, we report a sensitive and facile method to detect telomerase activity using AIEgens mod- ified probe （TPE-Py-DNA） as a fluorescence reporter and exonuclease llI （Exo lIl） as a signal amplifier. With the aid of telomerase, repeat units （TrAGGG）n are extended from the end of template substrate oligonucleotides （TS primer） that form duplex DNAs with TPE-Py-DNA. Then, Exo llI catalyzes the diges- tion of duplex DNAs, liberating elongation product and releasing hydrophobic TPE-Py. The released hydrophobic TPE-Py aggregate together and produce a telomerase-activity-related fluorescence signal. The liberated product hybridizes with another TPE-Py-DNA probe, starting the second cycle. Finally, we obtain the target-to-signal amplification ratio of 1 ：N2. This strategy exhibits good performance for detecting clinical urine samples （distinguishing 15 cancer patients＇ samples from 8 healthy ones） and checking intracellular telomerase activity （differentiating cell lines including HeLa, MDA-MB-231, MCF-7, A375, HLF and MRC-5 from the cells pretreated with telomerase-related drug）, which shows its potential in clinical diagnosis as well as therapeutic monitoring of cancer.展开更多
文摘In this study, five hundred urine samples and five hundred blood samples of abusers were examined for the presence of alkaloid substances and abuse drugs in urines and bloods. These numbers of blood and urine samples of addicts in clinics of welfare organization, during detoxification treatment or maintenance treatment were screened for abuse drugs presence. The all of samples were tested through as a view of clinical laboratory methods. Age ranges of female patients were 35 ~ 15 and age range of males patients were 45 ~ 15. All patients filled questionnaire and satisfy forms too. First, all fresh urine and blood samples were examined to confirm presence drugs abuses, depend on their addiction and treatment, so all samples were confirmed by two tests. Then they were examined to other clinical laboratory tests. All data were analyzed by t-test and were Anova one way and two ways of Anova Turkey, and p 〈 0.05 was considered significant. The p-value of this study was p = 0.0001. The results of this study were showed that 4% of abusers had mild increase in hematocrite level and 2% of narcotic drugs abusers had mild lower level of blood sugars than normal range and 4% of participants had increase liver enzymes such ALT （alanine transferase）, AST （aspartat transferase）, ALP （alkaline phosphatease） and 1% of them had renal failure. Although blood level BUN （blood urea nitrogen） and creatinin were examined to evaluation of their renal failure .The results in Tabriz/Iran undrevision of welfare organization clinics were approximately showed that positive results of addiction are in each of urine and blood samples. Because some of abusers directly consumed full long time agonist or partial agonists＇ drugs such as methadone and buprenorphine for their maintenance therapy in clinics. Also doing test on blood samples has high importance in distinguishing and confirmation of drugs abuse in samples. Also in this study we conclude that among all drug analytical methods the cheapest and easiest test to screening opioids and other abuse drugs in urine and blood samples is strip test for rapid diagnosis, also tests on blood samples have high importance as a view point of accuracy to distinguishing of drugs abuse, and serum levels of some other parameters showed all abusers patients situation such as liver and renal dysfimction through clinical laboratory tests.
基金This work was supported by ERAN I-21-18,Key Program of Tianjin Municipal Natural Science Foundation(19JCZDJC40500)Key Program of National Center for Occupational Safety and Health(2021-ZD-06).
文摘Objective:To establish a rapid analysis method for the activity concentration of carbon-14(14C)in urine,in order to estimate the internal dose of 14C exposure,and to protect the health of occupational population.Methods:Liquid scintillation counting(LSC)combined with the function of transformed spectral index of the external standard spectrum(tSIE)was used to measure the quenching level and counting rate,and the spiked urine samples with different shades of color were measured by LSC.After establishing the efficiency-quenching curve,the quenching correction and activity concentration analysis of the actual samples were carried out.Results:By LSC and the data fitting,the relationship between efficiency and quenching index could be represented using the equation y=0.0013x-0.0177(R^(2)=0.978).Three actual spiked samples were tested to verify this method,with recoveries of 97%,102%,and 89%,respectively.14C activity concentration of 4 actual urine samples were 0.12,0.11,0.10 and 0.08 Bq/mL,respectively,while the corresponding extended relative uncertainties were 0.0652,0.0929,0.0893 and 0.1043,respectively.Conclusion:The direct analysis method of 14C activity concentration in urine samples was established using LSC.The recovery of 14C activity concentration in urine samples showed that the proposed method had relatively high accuracy.By studying on the source of uncertainty,the uncertainty of the analysis results mainly came from the statistical error of LSC,and the uncertainty component of counting efficiency.
基金supported by Department of Analytical Chemistry,Faculty of Pharmacy,Zagazig University,Egypt.
文摘In this work,the application of silver nanoparticles(Ag NPs)for spectrophotometric determination of cefdinir(CFD)and cefepime HCl(CFP)was developed.The method was based on the ability of the alkaline degradation products of the cited drugs to reduce Ag+ions to silver nanoparticles(Ag-NPs)in the presence of gelatin as a capping agent.The linear concentration ranges were 5.0-40 and 10-100μg/mL for cefdinir and cefepime HCl,respectively.The lower detection limits were found to be 0.39 and 1.06μg/mL for CFD and CFP,respectively,while the quantitation limits were found to be 1.18 and 3.21μg/mL for CFD and CFP,respectively.The formed nanoparticles were characterized by ultraviolet-visible spectroscopy and transmission electron microscopy.The proposed method was applied successfully to determination of CFD and CFP in pure drugs,pharmaceutical preparations and human urine samples.
文摘In this study, one hundred urine samples and one hundred blood samples of abusers were examined for the presence of alkaloid substances and abuse drugs in urines and bloods. These numbers of blood and urine samples referred who addicts in clinics of Welfare Organization, during of detoxification treatment or maintenance treatment were screened for abuse drugs presence. Age ranges of female patients were 35 ± 15 and age range of males patients were 42 ± 18. All patients filled questionnaire and satisfy forms too. All data were analyzed by t-test and were Anowa one way, and P 〈 0.05 was considered significant. The P value of this study was P = 0.000. In this study we conclude that among all drug analytical methods the cheapest and easiest test to screening opioids and other abuse drugs in urine and blood samples is strip test for rapid diagnosis and TLC （thin-layer chromatography） is appropriate confirmation method to drug abuse distinguishing. Also tests on blood samples have high importance as a view point of accuracy to distinguishing of drugs abuse.
基金The work was supported by the Programme of Outstanding Talents in the New Century in Universities of Fujian,ChinaYoung Scientific Natural Science Foundation of Universities in Fujian.
文摘A new method was developed for pre-concentration and determination of multiple drugs of abuse in human urine using dispersive liquid–liquid microextraction(DLLME)and capillary electrophoresis(CE)with photodiode array detection.The method was based on the formation of tiny droplets of an organic extractant in the prepared sample solution using water-immiscible organic solvent(chloroform)dissolved in water-miscible organic dispersive solvent(isopropyl alcohol).The organic phase,which extracted eight drugs of abuse from the prepared urine solution,was separated by centrifugation.The sedimented phase was transferred into a small volume CE auto-sampler vial with 10μL of 1%HCl methanol solution and evaporated to dryness.The residue was reconstituted in lidocaine hydrochloride(internal standard)aqueous solution and introduced by electrokinetic injection into CE.Under the optimum conditions,acceptable linear relationship was observed in the range of 3.0–500 ng/mL with the correlation coefficient(r)of 0.9982–0.9994 for spiked urine samples.The limit of detection(LOD)(S/N=3)was estimated to be 1.0 ng/mL.A recovery of 75.7%–90.6%was obtained for spiked samples.The mean relative error(MRE)was within±7.0%and the relative standard deviation(RSD)was less than 6.9%.The proposed DLLME-CE procedure offers an alternative analytical approach for the sensitive detection of drugs of abuse in real urine samples.
文摘Introduction:On March 20,2025,a suspected Zika virus(ZIKV)case departed Thailand and flew to Nanning Wuxu International Airport before transiting to Jinan.Upon receiving notification,local CDCs immediately initiated comprehensive epidemiological investigations,laboratory testing,and preventive control measures.Methods:We collected urine,sputum,and blood samples from the patient for analysis.Quantitative real-time reverse-transcription polymerase chain reaction(qRT-PCR)was employed to detect ZIKV nucleic acid.Metagenome Next-Generation Sequencing(mNGS)was performed on the urine sample to obtain complete viral genome sequences.Phylogenetic analysis was subsequently constructed using the obtained sequences to determine the origin,genotype,and mutation profile of this imported case.Results:The qRT-PCR analysis confirmed ZIKV presence in the patient’s urine,sputum,and serum samples.The mNGS successfully generated the complete ZIKV genome sequence.Phylogenetic analysis demonstrated that the ZIKV strain belonged to the Asian lineage,exhibiting 99.57%nucleotide homology with a ZIKV strain from Bangkok,Thailand(GenBank accession no.OR264645.1).Conclusion:Based on the patient’s epidemiological history,clinical presentation,and nucleic acid test results from multiple specimens,this case was confirmed as the first imported ZIKV infection documented in Shandong Province,with the infection source traced to Thailand.
基金supported by the National Natural Science Foundation of China(No.42277424,No.42077385,and No.42477296)the Shenzhen Science and Technology Program(JCYJ20230807110903008 and JCYJ20240813150902004)+1 种基金Sanming Project of Medicine in Shenzhen(SZSM202211010)Shenzhen Key Medical Discipline Construction Fund(SZXK069).
文摘Bisphenol A(BPA)and its bisphenol analogs(BPs)serve as key precursors in the manufacturing of polycarbonate plastics and epoxy resins.However,due to BPA’s detrimental health effects,there has been a growing global consensus to restrict its use.The elderly,being particularly susceptible to neurodegenerative disorders,represent a population of concern;yet,research exploring the association between BPs exposure and neurodegenerative diseases in the elderly remains scarce.In this study,the urinary levels of six BPs among 267 participants from the Shenzhen aging-related disorder cohort in China during 2017–2018 were measured.Cognitive status was evaluated using the Mini-Mental State Examination(MMSE),and the impact of sociodemographic factors,lifestyle habits,and dietary practices was analyzed through the Extreme Gradient Boosting(XGBoost)classifier.In this study,BPA was the highest urinary level of bisphenol compound detected in the high-risk group(2.10 ng/mL,95.2%).Significant differences in BPs levels were observed to the degree of education,tea consumption frequency,meat intake frequency,and distance of main road groups(P<0.05).Notably,the absence of a significant positive correlation between BPF and BPA levels in high-risk groups for cognitive impairment implies more findings may be needed to fully evaluate BPF’s actual human exposure levels.The XGBoost models have further revealed that BPA analogs exposure and certain housing characteristics were substantial contributors to the potential impact on cognitive impairment during aging not only BPA.
基金supported by the National Natural Science Foundation of China(21375042,21405054,21525523,21574048,and21404028)the National Basic Research Program of China(2015CB932600,2013CB933000,and 2016YFF0100800)+1 种基金the Special Fund for Strategic New Industry Development of Shenzhen,China(JCYJ20150616144425376)1000 Young Talent Program(to F.Xia)
文摘Monitoring telomerase activity with high sensitive and reliable is of great importance to cancer analysis. In this paper, we report a sensitive and facile method to detect telomerase activity using AIEgens mod- ified probe （TPE-Py-DNA） as a fluorescence reporter and exonuclease llI （Exo lIl） as a signal amplifier. With the aid of telomerase, repeat units （TrAGGG）n are extended from the end of template substrate oligonucleotides （TS primer） that form duplex DNAs with TPE-Py-DNA. Then, Exo llI catalyzes the diges- tion of duplex DNAs, liberating elongation product and releasing hydrophobic TPE-Py. The released hydrophobic TPE-Py aggregate together and produce a telomerase-activity-related fluorescence signal. The liberated product hybridizes with another TPE-Py-DNA probe, starting the second cycle. Finally, we obtain the target-to-signal amplification ratio of 1 ：N2. This strategy exhibits good performance for detecting clinical urine samples （distinguishing 15 cancer patients＇ samples from 8 healthy ones） and checking intracellular telomerase activity （differentiating cell lines including HeLa, MDA-MB-231, MCF-7, A375, HLF and MRC-5 from the cells pretreated with telomerase-related drug）, which shows its potential in clinical diagnosis as well as therapeutic monitoring of cancer.
