Objective: To evaluate the anti-diabetic potential of leaf extract from Urena lobata(U. lobata) through dipeptidyl peptidase IV(DPP-IV) inhibitory activity.Methods: U. lobata leaf was extracted in hot water and ethano...Objective: To evaluate the anti-diabetic potential of leaf extract from Urena lobata(U. lobata) through dipeptidyl peptidase IV(DPP-IV) inhibitory activity.Methods: U. lobata leaf was extracted in hot water and ethanol. The activity of DPPIV inhibitor was tested by in vitro study using gly-pro-p-nitroanilide as substrat of DPPIV and vildagliptin, as standard reference. A product of the reactions between gly-pro-pnitroanilide and DPP-IV, was observed by microplate readers with λ = 405 nm. All data were expressed as mean ± SD and the IC50 value was determined by non linear regression curve fit. Active substances in leaf extract of U. lobata was analyzed by liquid chromatography-mass spectrometry. DPP-IV inhibitory activity of active compounds was evaluated in silico using docking server. Results: The ethanolic extract of U. lobata showed stronger DPP-IV inhibitor activity than water extract with the IC50 values of 1 654.64 and 6 489.88 μg/mL, respectively. Vildagliptin, based on standard reference for DPP-IV inhibitor activity, has IC50 value of 57.44 μg/mL. Based on in silico analysis, mangiferin, stigmasterol and β-sitosterol in U. lobata extract have a strong inhibitory activity on DPP-IV. Conclusions: The results showed that DPP-IV inhibitory activity of U. lobata is related to its active compounds such as mangiferin, stigmasterol and β-sitosterol.展开更多
Objective:To evaluate the possible antifertility activity of Enicostemma axillare(E.axillare) leaves and Urena lobata(U.lobata) root in adult male Wistar albino rats.Methods:Six groups of rats were treated with ethano...Objective:To evaluate the possible antifertility activity of Enicostemma axillare(E.axillare) leaves and Urena lobata(U.lobata) root in adult male Wistar albino rats.Methods:Six groups of rats were treated with ethanolic(70%v/v) extracts of E.axillare(375 and 750 mg/kg body weight) and U.lobata root(300 and 600 mg/kg body weight) once daily for 55 days.Control groups received the distilled water and vehicle.All the treated rats had corresponding recovery groups.At the end of each treatment periods,animals were killed and organ weights,sperm characteristics,testicular and epididymal biochemicals as well as testicular enzymes were assessed.Results:The E. axillare and U.lobata at tested doses did not decrease body weight,whereas the weight of testes, epididymides and seminal vesicles were significantly(P【0.01) reduced.Significantly(P【0.01) more reductions in the sperm motility,viability and counts,epididymal and testicular protein contents were noted in the rats treated with higher dose of both the plants.Both the plants at the higher dose caused a marked increase(P【0.01) in sperm morphological abnormalities,testicular cholesterol and ascorbic acid contents were remarkably increased(P【0.01),while,the activities of testicular glucose-6-phosphate dehydrogenase(G-6-PDH) andΔ~5-3β-hydroxy steroid dehydrogenase(Δ~5-3β-HSD) were significantly reduced(P【0.01).However,reversal of these changes occurred after 55 days of treatment withdrawal.Conclusions:This study suggests that the ? axillare leaves and U.lobata root reversibly inhibited spermatogenesis and steroidogenesis indicating reversible antifertility activity which could partially support the traditional of these plants as male contraceptives.展开更多
The aqueous and alcoholic extract of Urena lobata flowers has been used in acid/alkali titrations over a wide range of concentration. The results obtained with the flower extracts have been compared with those obtaine...The aqueous and alcoholic extract of Urena lobata flowers has been used in acid/alkali titrations over a wide range of concentration. The results obtained with the flower extracts have been compared with those obtained by using traditional chemical indicators like phenolphthalein and methyl orange. It has been established experimentally that the flower extract can be successfully used in place of phenolphthalein and methyl orange for acid/alkali titrations. The presence of anthocyanins is supposed to impart pH sensitive colour dependence to the natural indicator.展开更多
OBJECTIVE This study aimed to optimize polysaccharides extraction from Urena lobata L.and investigate its antioxidant activity.METHODS The mathematical model was established by re.sponse surface method(RSM) based on t...OBJECTIVE This study aimed to optimize polysaccharides extraction from Urena lobata L.and investigate its antioxidant activity.METHODS The mathematical model was established by re.sponse surface method(RSM) based on the results of single factor experiments,using polysaccha.rides extraction rate as response value,and using the ratio of water to material,cellulase concentra.tion,extraction temperature and time as experimental factors,which was used to screen optimum poly.saccharide extraction conditions from Urena lobata L..Antioxidant activity of polysaccharides was stud.ied by DPPH and ·OH free radical elimination method.RESULTS The optimum conditions obtained by RSM were as follows:the cellulase level was 10.8 g·L^(-1),extraction time duration was 72 min,the ra.tio of water to feedstock was 7 mL·g^(-1),extraction temperature was 43℃,the pH value was 5.0.Under the optimal conditions,there was a difference of less than 5% between predicted extraction rate 13.37% and experimental extraction rate 13.32%.The polysaccharide yield was most significantly af.fected by cellulase concentration,followed by extraction time,water to material ratio and extraction tem.perature.IC50 of DPPH and ·OH were 1.082 g·L^(-1) and 3.202 mg·L^(-1),respectively.Antioxidant activity of sample polysaccharides was weaker than those of vitamin C.CONCLUSION The polysaccharide extraction process from Urena lobata L.by cellulase enzymolysis approach was obtained,which was convenient and feasible,and extracted polysaccharides had good free radical scavenging activity.展开更多
基金Supported by a grant of doctoral dissertation research from Education Ministry of Indonesia
文摘Objective: To evaluate the anti-diabetic potential of leaf extract from Urena lobata(U. lobata) through dipeptidyl peptidase IV(DPP-IV) inhibitory activity.Methods: U. lobata leaf was extracted in hot water and ethanol. The activity of DPPIV inhibitor was tested by in vitro study using gly-pro-p-nitroanilide as substrat of DPPIV and vildagliptin, as standard reference. A product of the reactions between gly-pro-pnitroanilide and DPP-IV, was observed by microplate readers with λ = 405 nm. All data were expressed as mean ± SD and the IC50 value was determined by non linear regression curve fit. Active substances in leaf extract of U. lobata was analyzed by liquid chromatography-mass spectrometry. DPP-IV inhibitory activity of active compounds was evaluated in silico using docking server. Results: The ethanolic extract of U. lobata showed stronger DPP-IV inhibitor activity than water extract with the IC50 values of 1 654.64 and 6 489.88 μg/mL, respectively. Vildagliptin, based on standard reference for DPP-IV inhibitor activity, has IC50 value of 57.44 μg/mL. Based on in silico analysis, mangiferin, stigmasterol and β-sitosterol in U. lobata extract have a strong inhibitory activity on DPP-IV. Conclusions: The results showed that DPP-IV inhibitory activity of U. lobata is related to its active compounds such as mangiferin, stigmasterol and β-sitosterol.
文摘Objective:To evaluate the possible antifertility activity of Enicostemma axillare(E.axillare) leaves and Urena lobata(U.lobata) root in adult male Wistar albino rats.Methods:Six groups of rats were treated with ethanolic(70%v/v) extracts of E.axillare(375 and 750 mg/kg body weight) and U.lobata root(300 and 600 mg/kg body weight) once daily for 55 days.Control groups received the distilled water and vehicle.All the treated rats had corresponding recovery groups.At the end of each treatment periods,animals were killed and organ weights,sperm characteristics,testicular and epididymal biochemicals as well as testicular enzymes were assessed.Results:The E. axillare and U.lobata at tested doses did not decrease body weight,whereas the weight of testes, epididymides and seminal vesicles were significantly(P【0.01) reduced.Significantly(P【0.01) more reductions in the sperm motility,viability and counts,epididymal and testicular protein contents were noted in the rats treated with higher dose of both the plants.Both the plants at the higher dose caused a marked increase(P【0.01) in sperm morphological abnormalities,testicular cholesterol and ascorbic acid contents were remarkably increased(P【0.01),while,the activities of testicular glucose-6-phosphate dehydrogenase(G-6-PDH) andΔ~5-3β-hydroxy steroid dehydrogenase(Δ~5-3β-HSD) were significantly reduced(P【0.01).However,reversal of these changes occurred after 55 days of treatment withdrawal.Conclusions:This study suggests that the ? axillare leaves and U.lobata root reversibly inhibited spermatogenesis and steroidogenesis indicating reversible antifertility activity which could partially support the traditional of these plants as male contraceptives.
文摘The aqueous and alcoholic extract of Urena lobata flowers has been used in acid/alkali titrations over a wide range of concentration. The results obtained with the flower extracts have been compared with those obtained by using traditional chemical indicators like phenolphthalein and methyl orange. It has been established experimentally that the flower extract can be successfully used in place of phenolphthalein and methyl orange for acid/alkali titrations. The presence of anthocyanins is supposed to impart pH sensitive colour dependence to the natural indicator.
基金The project supported by Natural Science Foundation of Guangxi Zhuang Autonomous Region of China (2016GXNSFBA380040) and Scientific and Technological Research Projects of Yulin City of China (20173104 and 20173102)
文摘OBJECTIVE This study aimed to optimize polysaccharides extraction from Urena lobata L.and investigate its antioxidant activity.METHODS The mathematical model was established by re.sponse surface method(RSM) based on the results of single factor experiments,using polysaccha.rides extraction rate as response value,and using the ratio of water to material,cellulase concentra.tion,extraction temperature and time as experimental factors,which was used to screen optimum poly.saccharide extraction conditions from Urena lobata L..Antioxidant activity of polysaccharides was stud.ied by DPPH and ·OH free radical elimination method.RESULTS The optimum conditions obtained by RSM were as follows:the cellulase level was 10.8 g·L^(-1),extraction time duration was 72 min,the ra.tio of water to feedstock was 7 mL·g^(-1),extraction temperature was 43℃,the pH value was 5.0.Under the optimal conditions,there was a difference of less than 5% between predicted extraction rate 13.37% and experimental extraction rate 13.32%.The polysaccharide yield was most significantly af.fected by cellulase concentration,followed by extraction time,water to material ratio and extraction tem.perature.IC50 of DPPH and ·OH were 1.082 g·L^(-1) and 3.202 mg·L^(-1),respectively.Antioxidant activity of sample polysaccharides was weaker than those of vitamin C.CONCLUSION The polysaccharide extraction process from Urena lobata L.by cellulase enzymolysis approach was obtained,which was convenient and feasible,and extracted polysaccharides had good free radical scavenging activity.
