BACKGROUND Novel strategies are needed for improving guided bone regeneration(GBR) in oral surgery prior to implant placement, particularly in maxillary sinus augmentation(GBR-MSA) and in lateral alveolar ridge augmen...BACKGROUND Novel strategies are needed for improving guided bone regeneration(GBR) in oral surgery prior to implant placement, particularly in maxillary sinus augmentation(GBR-MSA) and in lateral alveolar ridge augmentation(LRA). This study tested the hypothesis that the combination of freshly isolated, unmodified autologous adipose-derived regenerative cells(UA-ADRCs), fraction 2 of plasma rich in growth factors(PRGF-2) and an osteoinductive scaffold(OIS)(UAADRC/PRGF-2/OIS) is superior to the combination of PRGF-2 and the same OIS alone(PRGF-2/OIS) in GBR-MSA/LRA.CASE SUMMARY A 79-year-old patient was treated with a bilateral external sinus lift procedure as well as a bilateral lateral alveolar ridge augmentation. GBR-MSA/LRA was performed with UA-ADRC/PRGF-2/OIS on the right side, and with PRGF-2/OIS on the left side. Biopsies were collected at 6 wk and 34 wk after GBRMSA/LRA. At the latter time point implants were placed. Radiographs(32 mo follow-up time) demonstrated excellent bone healing. No radiological or histological signs of inflammation were observed. Detailed histologic,histomorphometric, and immunohistochemical analysis of the biopsies evidenced that UA-ADRC/PRGF-2/OIS resulted in better and faster bone regeneration than PRGF-2/OIS.CONCLUSION GBR-MSA with UA-ADRCs, PRGF-2, and an OIS shows effectiveness without adverse effects.展开更多
Target discovery,involving target identification and validation,is the prerequisite for drug discovery and screening.Novel methodologies and technologies for the precise discovery and confirmation of drug targets are ...Target discovery,involving target identification and validation,is the prerequisite for drug discovery and screening.Novel methodologies and technologies for the precise discovery and confirmation of drug targets are powerful tools in understanding the disease,looking for a drug and elucidating the mechanism of drug treatment.Among the common target identification and confirmation methods,the modified method is time-consuming and laborious,which may reduce or change the activity of natural products.The unmodified methods developed in recent years without chemical modification have gradually become an important means of studying drug targets.A wide range of unmodified approaches have been reported,introducing and analyzing the recent emerging methodologies and technologies.This review highlights the advantages and limitations of these methods for the application of drug target discovery and presents an overview of their contributions to the target discovery of small molecule drugs.The application and future development trends of methodologies in target discovery are also prospected to provide a reference for drug target research.展开更多
Three new N-alkylsulfonamide-containing phenylazopyrazolone dyes have been synthesized by reaction of sulfonyl chloride intermediates with different saturated aliphatic amines and their molecular structures were confi...Three new N-alkylsulfonamide-containing phenylazopyrazolone dyes have been synthesized by reaction of sulfonyl chloride intermediates with different saturated aliphatic amines and their molecular structures were confirmed by FTIR, (1)H NMR and MS. The dyes were applied to unmodified polypropylene fabric. These dyes showed high exhaustion as well as higher levels of fastness to washing, sublimation and rubbing compared with C.I. Disperse Yellow 16. The results showed the N-alkylsulfonamide-containing dyes were suitable for dyeing unmodified PP fiber and exhibited good color depth and fastness properties.展开更多
A simple visual method for DNA detection during the formation of gold nanoparticles(AuNPs) was developed based on different electrostatic properties of single strand DNA(ssDNA) and double strand DNA(dsDNA).Since the s...A simple visual method for DNA detection during the formation of gold nanoparticles(AuNPs) was developed based on different electrostatic properties of single strand DNA(ssDNA) and double strand DNA(dsDNA).Since the ssDNA is easy to bind to AuNPs due to its exposed bases which could prevent saltinduced aggregation of AuNPs.The dsDNA always present negative charge because its negatively charged phosphate backbone is exposed.In this case,the dsDNA could disturb the adsorption between dsDNA and AuNPs and result in non-aggregation of AuNPs.After hybridization,chloroauric acid and ascorbic acid were added to the mixture solution,and the solution changed to red immediately and turned to purple in10 min in the present of target DNA.TEM results confirmed that the change of color stemed from aggregation of AuNPs.In order to obtain accurate results by naked eye,the DNA detection assay should be conducted under pH 7.0.展开更多
The aim of this study is to clarify the effect of different concentrations of titanium dioxide nanoparticles (Nps) on the properties of two types of heat polymerized acrylic resin. The tested parameters were flexural ...The aim of this study is to clarify the effect of different concentrations of titanium dioxide nanoparticles (Nps) on the properties of two types of heat polymerized acrylic resin. The tested parameters were flexural strength, impact strength, and microhardness. The two types of acrylic resin used in this study were conventional unmodified (Implacryl, Vertex) and high impact heat polymerized acrylic resin (Vertex-Dental, Netherlands). Both types of acrylic resin were modified by using 1% and 5% TiO<sub>2</sub> Nps powder. Specimen’s dimensions were prepared according to the American Dental Association Specification No. 12. Three types of specimens were prepared: 1) flexural strength specimens 50 mm × 10 (±0.2) mm × 3 (±0.2) mm, 2) impact strength test specimens 60 mm × 6.0 mm × 4.0 mm, 3) microhardnesss specimens 25 mm × 10 mm × 3 (±0.2) mm. For each test 6 groups were prepared (each group containing 5 samples). Thirty specimens were prepared in each of the three tests, amounting to a total number of 90 specimens. Mechanical properties such as flexural strength (FS), impact strength and microhardness of the above mentioned specimens were determined using universal testing machine, Izod pendulum impact testing machine and Vickers microhardness tester, respectively. ISO Specification No. 1567 was followed in microhardness test. The data was collected and statistically analyzed. Flexural strength considerably decreased by increasing TiO<sub>2</sub> concentration in both types of acrylic resin. Impact strength of the conventional acrylic resin modified by 1% of additives significantly increased. The microhardness is significantly increased by addition of 5% of TiO<sub>2</sub> Nps. The Incorporation of TiO<sub>2</sub> nanoparticles into acrylic resins can adversely affect its flexural strength. Meanwhile, the impact strength can be modified by small percentage of additives (abt. 1%). This effect is directly correlated with the concentration of nanoparticles. On the other hand, concentrations of TiO<sub>2</sub> Nps (abt. 5%) positively affect the microhardness of both types of acrylic resin used in the present study.</sub></sub></sub></sub></sub></sub></sub></sub></sub></sub>展开更多
Water pollution accidents, such as the Flint water crisis in the United States, caused by lead contamination have raised concern on the safety of drinking water distribution systems. Thus, the routine monitoring of le...Water pollution accidents, such as the Flint water crisis in the United States, caused by lead contamination have raised concern on the safety of drinking water distribution systems. Thus, the routine monitoring of lead in water is highly required and demands efficient, sensitive, cost-effective, and reliable lead detection methods. This study reports a label-free colorimetric nanosensor that uses unmodified gold nanoparticles (AuNPs) as indicators to enable rapid and ultra-sensitive detection of lead in environmental water. The 8-17 DNAzyme was truncated in this study to facilitate the detachment of single-stranded DNA fragments after substrate cleavage in the presence of Pb「. The detached fragments were adsorbed over AuNPs and protected against salt concentration-induced aggregation. Accordingly, high Pb^2+ would result in rapid color change from blue to pink. The established sensing principle achieved a sensitive limit of detection of 0.2×10^-9mol/L Pb^2+. with a linear working range of two orders of magnitude from 0.5×10^-9mol/L to 5×10^-9mol/L. The selectivity of the nanosensor was demonstrated by evaluating the interfering metal ions. The developed nanosensor can serve as a substitute for the rapid analysis and monitoring of trace lead levels under the drinking water distribution system and even other environmental water samples.展开更多
Interaction of unmodified starch with vip molecules or ligands(e.g.,fatty acids)as a basis for the formation of starch-encapsulated mineral filler particles is an effective process for mitigating the negative impact...Interaction of unmodified starch with vip molecules or ligands(e.g.,fatty acids)as a basis for the formation of starch-encapsulated mineral filler particles is an effective process for mitigating the negative impact of filler addition on the strength properties of cellulosic networks.As unmodified starch is essentially nonionic,the interaction of starch-engineered fillers with negatively charged cellulosic fibers is somehow limited.Here,the concept of substituting unmodified starch with a minor amount of cationic starch in filler engineering with starch inclusion complexes was proposed.It was hypothesized that filler-fiber interaction would be enhanced by cationic-anionic attraction.Encouragingly,the effectiveness of this concept was demonstrated to be very pronounced.For instance,at a cationic starch percentage of 3%(relevant to the weight of total starch),filler retention and filler bondability with cellulosic fibers were significantly improved,leading to further mitigated negative impact of filler addition on tensile strength.Basically,this easily scalable concept may shed light on greener,more efficient use of filler technologies on the basis of starch inclusion complex formation,opening up new possibilities for real commercial applications.展开更多
文摘BACKGROUND Novel strategies are needed for improving guided bone regeneration(GBR) in oral surgery prior to implant placement, particularly in maxillary sinus augmentation(GBR-MSA) and in lateral alveolar ridge augmentation(LRA). This study tested the hypothesis that the combination of freshly isolated, unmodified autologous adipose-derived regenerative cells(UA-ADRCs), fraction 2 of plasma rich in growth factors(PRGF-2) and an osteoinductive scaffold(OIS)(UAADRC/PRGF-2/OIS) is superior to the combination of PRGF-2 and the same OIS alone(PRGF-2/OIS) in GBR-MSA/LRA.CASE SUMMARY A 79-year-old patient was treated with a bilateral external sinus lift procedure as well as a bilateral lateral alveolar ridge augmentation. GBR-MSA/LRA was performed with UA-ADRC/PRGF-2/OIS on the right side, and with PRGF-2/OIS on the left side. Biopsies were collected at 6 wk and 34 wk after GBRMSA/LRA. At the latter time point implants were placed. Radiographs(32 mo follow-up time) demonstrated excellent bone healing. No radiological or histological signs of inflammation were observed. Detailed histologic,histomorphometric, and immunohistochemical analysis of the biopsies evidenced that UA-ADRC/PRGF-2/OIS resulted in better and faster bone regeneration than PRGF-2/OIS.CONCLUSION GBR-MSA with UA-ADRCs, PRGF-2, and an OIS shows effectiveness without adverse effects.
基金supported by grants from the National Natural Science Foundation of China(No.31870946)the Funding of Double First-rate discipline construction(No.CPU2018GF07)+1 种基金the Priority Academic Program Development of Jiangsu Higher Education Institutionsthe Open Project Program of MOE Key Laboratory of Drug Quality Control and Pharmacovigilance(No.DQCP20/21MS01).
文摘Target discovery,involving target identification and validation,is the prerequisite for drug discovery and screening.Novel methodologies and technologies for the precise discovery and confirmation of drug targets are powerful tools in understanding the disease,looking for a drug and elucidating the mechanism of drug treatment.Among the common target identification and confirmation methods,the modified method is time-consuming and laborious,which may reduce or change the activity of natural products.The unmodified methods developed in recent years without chemical modification have gradually become an important means of studying drug targets.A wide range of unmodified approaches have been reported,introducing and analyzing the recent emerging methodologies and technologies.This review highlights the advantages and limitations of these methods for the application of drug target discovery and presents an overview of their contributions to the target discovery of small molecule drugs.The application and future development trends of methodologies in target discovery are also prospected to provide a reference for drug target research.
文摘Three new N-alkylsulfonamide-containing phenylazopyrazolone dyes have been synthesized by reaction of sulfonyl chloride intermediates with different saturated aliphatic amines and their molecular structures were confirmed by FTIR, (1)H NMR and MS. The dyes were applied to unmodified polypropylene fabric. These dyes showed high exhaustion as well as higher levels of fastness to washing, sublimation and rubbing compared with C.I. Disperse Yellow 16. The results showed the N-alkylsulfonamide-containing dyes were suitable for dyeing unmodified PP fiber and exhibited good color depth and fastness properties.
基金financially supported by the National Key Research and Development Program of China (No.2017YFA0205301)the National Natural Science Foundation of China (Nos.61527806, 81902153 and 61871180)+2 种基金the Natural Science Foundation of Hunan Province (No.2017JJ2069)the Hunan Key Research Project (No.2017SK2174)the Programs for Changjiang Scholars and Innovative Research Team in University (No.IRT_15R13)
文摘A simple visual method for DNA detection during the formation of gold nanoparticles(AuNPs) was developed based on different electrostatic properties of single strand DNA(ssDNA) and double strand DNA(dsDNA).Since the ssDNA is easy to bind to AuNPs due to its exposed bases which could prevent saltinduced aggregation of AuNPs.The dsDNA always present negative charge because its negatively charged phosphate backbone is exposed.In this case,the dsDNA could disturb the adsorption between dsDNA and AuNPs and result in non-aggregation of AuNPs.After hybridization,chloroauric acid and ascorbic acid were added to the mixture solution,and the solution changed to red immediately and turned to purple in10 min in the present of target DNA.TEM results confirmed that the change of color stemed from aggregation of AuNPs.In order to obtain accurate results by naked eye,the DNA detection assay should be conducted under pH 7.0.
文摘The aim of this study is to clarify the effect of different concentrations of titanium dioxide nanoparticles (Nps) on the properties of two types of heat polymerized acrylic resin. The tested parameters were flexural strength, impact strength, and microhardness. The two types of acrylic resin used in this study were conventional unmodified (Implacryl, Vertex) and high impact heat polymerized acrylic resin (Vertex-Dental, Netherlands). Both types of acrylic resin were modified by using 1% and 5% TiO<sub>2</sub> Nps powder. Specimen’s dimensions were prepared according to the American Dental Association Specification No. 12. Three types of specimens were prepared: 1) flexural strength specimens 50 mm × 10 (±0.2) mm × 3 (±0.2) mm, 2) impact strength test specimens 60 mm × 6.0 mm × 4.0 mm, 3) microhardnesss specimens 25 mm × 10 mm × 3 (±0.2) mm. For each test 6 groups were prepared (each group containing 5 samples). Thirty specimens were prepared in each of the three tests, amounting to a total number of 90 specimens. Mechanical properties such as flexural strength (FS), impact strength and microhardness of the above mentioned specimens were determined using universal testing machine, Izod pendulum impact testing machine and Vickers microhardness tester, respectively. ISO Specification No. 1567 was followed in microhardness test. The data was collected and statistically analyzed. Flexural strength considerably decreased by increasing TiO<sub>2</sub> concentration in both types of acrylic resin. Impact strength of the conventional acrylic resin modified by 1% of additives significantly increased. The microhardness is significantly increased by addition of 5% of TiO<sub>2</sub> Nps. The Incorporation of TiO<sub>2</sub> nanoparticles into acrylic resins can adversely affect its flexural strength. Meanwhile, the impact strength can be modified by small percentage of additives (abt. 1%). This effect is directly correlated with the concentration of nanoparticles. On the other hand, concentrations of TiO<sub>2</sub> Nps (abt. 5%) positively affect the microhardness of both types of acrylic resin used in the present study.</sub></sub></sub></sub></sub></sub></sub></sub></sub></sub>
文摘Water pollution accidents, such as the Flint water crisis in the United States, caused by lead contamination have raised concern on the safety of drinking water distribution systems. Thus, the routine monitoring of lead in water is highly required and demands efficient, sensitive, cost-effective, and reliable lead detection methods. This study reports a label-free colorimetric nanosensor that uses unmodified gold nanoparticles (AuNPs) as indicators to enable rapid and ultra-sensitive detection of lead in environmental water. The 8-17 DNAzyme was truncated in this study to facilitate the detachment of single-stranded DNA fragments after substrate cleavage in the presence of Pb「. The detached fragments were adsorbed over AuNPs and protected against salt concentration-induced aggregation. Accordingly, high Pb^2+ would result in rapid color change from blue to pink. The established sensing principle achieved a sensitive limit of detection of 0.2×10^-9mol/L Pb^2+. with a linear working range of two orders of magnitude from 0.5×10^-9mol/L to 5×10^-9mol/L. The selectivity of the nanosensor was demonstrated by evaluating the interfering metal ions. The developed nanosensor can serve as a substitute for the rapid analysis and monitoring of trace lead levels under the drinking water distribution system and even other environmental water samples.
基金support from the Fundamental Research Funds for the Central Universities of China (2572015DB01)the Program for New Century Excellent Talents in University (NCET-12-0811)the National Natural Science Foundation of China (31100439).
文摘Interaction of unmodified starch with vip molecules or ligands(e.g.,fatty acids)as a basis for the formation of starch-encapsulated mineral filler particles is an effective process for mitigating the negative impact of filler addition on the strength properties of cellulosic networks.As unmodified starch is essentially nonionic,the interaction of starch-engineered fillers with negatively charged cellulosic fibers is somehow limited.Here,the concept of substituting unmodified starch with a minor amount of cationic starch in filler engineering with starch inclusion complexes was proposed.It was hypothesized that filler-fiber interaction would be enhanced by cationic-anionic attraction.Encouragingly,the effectiveness of this concept was demonstrated to be very pronounced.For instance,at a cationic starch percentage of 3%(relevant to the weight of total starch),filler retention and filler bondability with cellulosic fibers were significantly improved,leading to further mitigated negative impact of filler addition on tensile strength.Basically,this easily scalable concept may shed light on greener,more efficient use of filler technologies on the basis of starch inclusion complex formation,opening up new possibilities for real commercial applications.
