6-（5-Carboxyl-2-methoxyphenyl）-apigenin （1）, a new flavonoid, was isolated from the 60% ethanol extract of Selaginella uncinata （Desv.） Spring. Its structure was established by spectroscopic methods. Compound 1 ...6-（5-Carboxyl-2-methoxyphenyl）-apigenin （1）, a new flavonoid, was isolated from the 60% ethanol extract of Selaginella uncinata （Desv.） Spring. Its structure was established by spectroscopic methods. Compound 1 represents the first example of the flavonoids possessing a benzoic acid substituent at C-6.展开更多
Two new chromone glycosides, 5-hydroxy-2,6,8-trimethylchromon-7-O-b-D-gluco- pyranoside, named uncinoside A; 5-acetoxy-2,6,8-trimethylchromone-7-O-b-D-glucopyranoside, named uncinoside B, and a known chromone compound...Two new chromone glycosides, 5-hydroxy-2,6,8-trimethylchromon-7-O-b-D-gluco- pyranoside, named uncinoside A; 5-acetoxy-2,6,8-trimethylchromone-7-O-b-D-glucopyranoside, named uncinoside B, and a known chromone compound named 8-methyl eugenitol were isolated from Selaginella uncinata. Their structures were elucidated by spectra analysis of FAB-MS, 1D NMR and 2D NMR including 1H NMR, 13C NMR, HMQC, HMBC and single-crystal X-ray diffraction techniques.展开更多
Morphogenesis of cell division is investigated in Uronychia uncinata based on protargol impregnated apecimens. The conical morphogenetic events are similar to that known in other euplotids but exhibiting several chara...Morphogenesis of cell division is investigated in Uronychia uncinata based on protargol impregnated apecimens. The conical morphogenetic events are similar to that known in other euplotids but exhibiting several characteristies: the oral primordium in proter being de novo developed in a subcortical pouch anterior to the parental AZM2; FC comes from anlage Ⅰ-Ⅲ; all frontoventral and transverse cirri in both proter and opisthe deriving from 5 cirral streaks; development of proter oral primordia causing partial replacement of old AZM1; caudal cirri coming from two DK anlagen.展开更多
Three new carboxylated flavonoids, uncinatic acids A-C（1–3）, were isolated from the whole herb of Selaginella uncinata. Their structures were established on the basis of extensive NMR analysis including1 D, 2D NMR ...Three new carboxylated flavonoids, uncinatic acids A-C（1–3）, were isolated from the whole herb of Selaginella uncinata. Their structures were established on the basis of extensive NMR analysis including1 D, 2D NMR experiments and HR-ESIMS techniques. All of them share the carboxylation structural characteristic. Compounds 1 and 2 belong to novel naturally occuring furanoflavonoids which is firstly reported in genus Selaginella. Such furanoflavonoids with dicarboxylic acid structrure have never been discovered before. In addition, the isolates were tested for their cytotoxicity against A549 and BGC-823 cell lines in vitro.展开更多
Inflammatory bowel disease(IBD)is a serious disorder,and exploration of active compounds to treat it is necessary.An acidic polysaccharide named SUSP-4 was purified from Selaginella uncinata(Desv.)Spring,which contain...Inflammatory bowel disease(IBD)is a serious disorder,and exploration of active compounds to treat it is necessary.An acidic polysaccharide named SUSP-4 was purified from Selaginella uncinata(Desv.)Spring,which contained galacturonic acid,galactose,xylose,arabinose,and rhamnose with the main chain structure of→4)-α-d-GalAp-(1→and→6)-β-d-Galp-(1→and the branched structure of→5)-α-l-Araf-(1→.Animal experiments showed that compared with Model group,SUSP-4 significantly improved body weight status,disease activity index(DAI),colonic shortening,and histopathological damage,and elevated occludin and zonula occludens protein 1(ZO-1)expression in mice induced by dextran sulfate sodium salt(DSS).16S ribosomal RNA(rRNA)sequencing indicated that SUSP-4 markedly downregulated the level of Akkermansia and Alistipes.Metabolomics results confirmed that SUSP-4 obviously elevated thiamine levels compared with Model mice by adjusting thiamine metabolism,which was further confirmed by a targeted metabolism study.Fecal transplantation experiments showed that SUSP-4 exerted an anti-IBD effect by altering the intestinal flora in mice.A mechanistic study showed that SUSP-4 markedly inhibited macrophage activation by decreasing the levels of phospho-nuclear factor kappa-B(p-NF-κB)and cyclooxygenase-2(COX-2)and elevating NF-E2-related factor 2(Nrf2)levels compared with Model group.In conclusion,SUSP-4 affected thiamine metabolism by regulating Akkermania and inhibited macrophage activation to adjust NF-κB/Nrf2/COX-2-mediated inflammation and oxidative stress against IBD.This is the first time that plant polysaccharides have been shown to affect thiamine metabolism against IBD,showing great potential for in-depth research and development applications.展开更多
基金the National Basic Research Program of China(No.2006CB504100).
文摘6-（5-Carboxyl-2-methoxyphenyl）-apigenin （1）, a new flavonoid, was isolated from the 60% ethanol extract of Selaginella uncinata （Desv.） Spring. Its structure was established by spectroscopic methods. Compound 1 represents the first example of the flavonoids possessing a benzoic acid substituent at C-6.
文摘Two new chromone glycosides, 5-hydroxy-2,6,8-trimethylchromon-7-O-b-D-gluco- pyranoside, named uncinoside A; 5-acetoxy-2,6,8-trimethylchromone-7-O-b-D-glucopyranoside, named uncinoside B, and a known chromone compound named 8-methyl eugenitol were isolated from Selaginella uncinata. Their structures were elucidated by spectra analysis of FAB-MS, 1D NMR and 2D NMR including 1H NMR, 13C NMR, HMQC, HMBC and single-crystal X-ray diffraction techniques.
文摘Morphogenesis of cell division is investigated in Uronychia uncinata based on protargol impregnated apecimens. The conical morphogenetic events are similar to that known in other euplotids but exhibiting several characteristies: the oral primordium in proter being de novo developed in a subcortical pouch anterior to the parental AZM2; FC comes from anlage Ⅰ-Ⅲ; all frontoventral and transverse cirri in both proter and opisthe deriving from 5 cirral streaks; development of proter oral primordia causing partial replacement of old AZM1; caudal cirri coming from two DK anlagen.
基金supported by grants from the National Natural Science Foundation of China(No.31370370)Key Project of Science and Technology of Hunan Province,China(Nos.2014SK2002 and 2013SK5077)+1 种基金Key Project of Application Technology Research and Development of Haikou(No.2015-039)Social Development and Technology Specific Program of Hainan Province(No.SF201419)
文摘Three new carboxylated flavonoids, uncinatic acids A-C（1–3）, were isolated from the whole herb of Selaginella uncinata. Their structures were established on the basis of extensive NMR analysis including1 D, 2D NMR experiments and HR-ESIMS techniques. All of them share the carboxylation structural characteristic. Compounds 1 and 2 belong to novel naturally occuring furanoflavonoids which is firstly reported in genus Selaginella. Such furanoflavonoids with dicarboxylic acid structrure have never been discovered before. In addition, the isolates were tested for their cytotoxicity against A549 and BGC-823 cell lines in vitro.
基金the funding from the Spring City Plan of the High-Level Talent Promotion and Training Project of Kunming,China(Grant No.:2022SCP008)the Independent Research Fund of Yunnan Characteristic Plant Extraction Laboratory,China(Grant No.:2022YKZY001).
文摘Inflammatory bowel disease(IBD)is a serious disorder,and exploration of active compounds to treat it is necessary.An acidic polysaccharide named SUSP-4 was purified from Selaginella uncinata(Desv.)Spring,which contained galacturonic acid,galactose,xylose,arabinose,and rhamnose with the main chain structure of→4)-α-d-GalAp-(1→and→6)-β-d-Galp-(1→and the branched structure of→5)-α-l-Araf-(1→.Animal experiments showed that compared with Model group,SUSP-4 significantly improved body weight status,disease activity index(DAI),colonic shortening,and histopathological damage,and elevated occludin and zonula occludens protein 1(ZO-1)expression in mice induced by dextran sulfate sodium salt(DSS).16S ribosomal RNA(rRNA)sequencing indicated that SUSP-4 markedly downregulated the level of Akkermansia and Alistipes.Metabolomics results confirmed that SUSP-4 obviously elevated thiamine levels compared with Model mice by adjusting thiamine metabolism,which was further confirmed by a targeted metabolism study.Fecal transplantation experiments showed that SUSP-4 exerted an anti-IBD effect by altering the intestinal flora in mice.A mechanistic study showed that SUSP-4 markedly inhibited macrophage activation by decreasing the levels of phospho-nuclear factor kappa-B(p-NF-κB)and cyclooxygenase-2(COX-2)and elevating NF-E2-related factor 2(Nrf2)levels compared with Model group.In conclusion,SUSP-4 affected thiamine metabolism by regulating Akkermania and inhibited macrophage activation to adjust NF-κB/Nrf2/COX-2-mediated inflammation and oxidative stress against IBD.This is the first time that plant polysaccharides have been shown to affect thiamine metabolism against IBD,showing great potential for in-depth research and development applications.
