Ultraviolet(UV)phototherapy stands as a pivotal clinical approach for managing diverse skin diseases.However,its application is constrained by concerns over UV-induced toxicity and imprecise photosensitizer delivery.T...Ultraviolet(UV)phototherapy stands as a pivotal clinical approach for managing diverse skin diseases.However,its application is constrained by concerns over UV-induced toxicity and imprecise photosensitizer delivery.This work introduces a light-converting microneedle patch(EU-MN),exemplified by its remarkable efficacy in psoriasis treatment.The EU-MN patch could release upconversion nanoparticles(UCNPs)and the photosensitizer methoxypsoralen(MOP)in controlled amounts in response to elevated matrix metalloproteinase(MMP)levels within the skin.Under near-infrared(NIR)excitation,UCNPs emit UV light(345/361 nm),which is combined with MOP to achieve more precise intradermal UV photoche-motherapy,effectively inhibiting abnormal proliferation of human immortalized epidermal cells(HaCaT)and bacterial growth(Staphylococcus aureus and Escherichia coli).Comet assay highlights DNA damage correlation.In addition,the microneedles,designed with a reactive oxygen species(ROS)-responsive shell,release epigallocatechin gallate(EGCG)to counteract excessive inflammation and mitigate UV-induced damage.In psoriasis mice,the EU-MN patch demonstrates significant therapeutic efficacy and recurrence prevention.As further evidenced by the suppression of epidermal hyperplasia and inflammation(through RNA sequencing identifying cell cycle arrest),this EU-MN patch offers a safer,more precise,and more effective alternative strategy for conventional direct UV phototherapy.展开更多
To investigate the effects of ultraviolet blood irradiation and oxygenation (UBIO) on free radicals and antioxidase after spinal cord injury in rabbits Methods Totally, 186 rabbits were used and divided randomly in...To investigate the effects of ultraviolet blood irradiation and oxygenation (UBIO) on free radicals and antioxidase after spinal cord injury in rabbits Methods Totally, 186 rabbits were used and divided randomly into four experimental groups: control (n=6), blood transfusion (n=24), injured (n=96) and treatment (n=60) groups The relative intensity of free radical (FR) signals, malondialdehyde (MDA) content, as well as the activity of superoxide dismutase (SOD) and glutathione peroxidase (GSH PX) were compared among the four groups at 6, 24, 48, and 72 hours and 6 days after injury Results The relative intensity of FR signals in spinal cord tissue in the injured group increased at 48 hours and showed a striking difference compared with the control group; in the treatment group, it decreased and showed a striking difference compared with the injured group MDA content in blood in the injured group increased and showed a striking difference at 6, 24 and 48 hours and showed a significant difference at 72 hours and 6 days after injury compared with the control group In the treatment group, MDA content in blood decreased and showed a significant difference at 48 hours compared with the injured group MDA content in spinal cord tissue increased in the injured group and showed a striking difference compared with the control group; in the treatment group, it decreased and showed a striking difference compared with the injured group at the corresponding times The activity of SOD in blood and spinal cord tissue decreased in the injured group and showed a striking difference compared with the control group; in the treatment group, it increased and showed a striking difference compared with the injured group at the corresponding times The changes in activity of GSH PX in blood and spinal cord tissue were similar to that in SOD No significant difference was observed between the blood transfusion and control groups Conclusion UBIO can ease free radical damages and elevate the activity of antioxidases after spinal cord injury in rabbits展开更多
基金funded by the Jiangxi Province Key Laboratory of Precision Cell Therapy(No.2024SSY06241 to J.L.)the National Natural Science Foundation of China(No.82360174 to J.L.)+3 种基金the Science and Technology Department Project of Jiangxi Province(No.20224ABC03A02 to J.L.)the Key Research and Development Program of Jiangxi Province(No.20212BBG73004 to X.L.W.)the Jiangxi Province Key Laboratory of Bioengineering Drugs(No.2024SSY07061 to X.L.W.)the Graduate Innovation Special Fund Project of Jiangxi Province(No.YC2023-B046 to L.B.L.).
文摘Ultraviolet(UV)phototherapy stands as a pivotal clinical approach for managing diverse skin diseases.However,its application is constrained by concerns over UV-induced toxicity and imprecise photosensitizer delivery.This work introduces a light-converting microneedle patch(EU-MN),exemplified by its remarkable efficacy in psoriasis treatment.The EU-MN patch could release upconversion nanoparticles(UCNPs)and the photosensitizer methoxypsoralen(MOP)in controlled amounts in response to elevated matrix metalloproteinase(MMP)levels within the skin.Under near-infrared(NIR)excitation,UCNPs emit UV light(345/361 nm),which is combined with MOP to achieve more precise intradermal UV photoche-motherapy,effectively inhibiting abnormal proliferation of human immortalized epidermal cells(HaCaT)and bacterial growth(Staphylococcus aureus and Escherichia coli).Comet assay highlights DNA damage correlation.In addition,the microneedles,designed with a reactive oxygen species(ROS)-responsive shell,release epigallocatechin gallate(EGCG)to counteract excessive inflammation and mitigate UV-induced damage.In psoriasis mice,the EU-MN patch demonstrates significant therapeutic efficacy and recurrence prevention.As further evidenced by the suppression of epidermal hyperplasia and inflammation(through RNA sequencing identifying cell cycle arrest),this EU-MN patch offers a safer,more precise,and more effective alternative strategy for conventional direct UV phototherapy.
文摘To investigate the effects of ultraviolet blood irradiation and oxygenation (UBIO) on free radicals and antioxidase after spinal cord injury in rabbits Methods Totally, 186 rabbits were used and divided randomly into four experimental groups: control (n=6), blood transfusion (n=24), injured (n=96) and treatment (n=60) groups The relative intensity of free radical (FR) signals, malondialdehyde (MDA) content, as well as the activity of superoxide dismutase (SOD) and glutathione peroxidase (GSH PX) were compared among the four groups at 6, 24, 48, and 72 hours and 6 days after injury Results The relative intensity of FR signals in spinal cord tissue in the injured group increased at 48 hours and showed a striking difference compared with the control group; in the treatment group, it decreased and showed a striking difference compared with the injured group MDA content in blood in the injured group increased and showed a striking difference at 6, 24 and 48 hours and showed a significant difference at 72 hours and 6 days after injury compared with the control group In the treatment group, MDA content in blood decreased and showed a significant difference at 48 hours compared with the injured group MDA content in spinal cord tissue increased in the injured group and showed a striking difference compared with the control group; in the treatment group, it decreased and showed a striking difference compared with the injured group at the corresponding times The activity of SOD in blood and spinal cord tissue decreased in the injured group and showed a striking difference compared with the control group; in the treatment group, it increased and showed a striking difference compared with the injured group at the corresponding times The changes in activity of GSH PX in blood and spinal cord tissue were similar to that in SOD No significant difference was observed between the blood transfusion and control groups Conclusion UBIO can ease free radical damages and elevate the activity of antioxidases after spinal cord injury in rabbits
