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Surface integrity characterization of thirdgeneration nickel-based single crystal blade tenons after ultrasonic vibration-assisted grinding
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作者 Biao ZHAO Hexu YOU +3 位作者 Qing MIAO Wenfeng DING Ning QIAN Jiuhua XU 《Chinese Journal of Aeronautics》 2025年第1期235-253,共19页
Machined surface integrity of workpieces in harsh environments has a remarkable influence on their performance.However,the complexity of the new type of machining hinders a comprehensive understanding of machined surf... Machined surface integrity of workpieces in harsh environments has a remarkable influence on their performance.However,the complexity of the new type of machining hinders a comprehensive understanding of machined surface integrity and its formation mechanism,thereby limiting the study of component performance.With increasing demands for high-quality machined workpieces in aerospace industry applications,researchers from academia and industry are increasingly focusing on post-machining surface characterization.The profile grinding test was conducted on a novel single-crystal superalloy to simulate the formation of blade tenons,and the obtained tenons were characterized for surface integrity elements under various operating conditions.Results revealed that ultrasonic vibration-assisted grinding(UVAG)led to multiple superpositions of abrasive grain trajectories,causing reduced surface roughness(an average reduction of approximately29.6%)compared with conventional grinding.After examining the subsurface layer of UVAG using transmission electron microscopy,the results revealed that the single-crystal tenon grinding subsurface layer exhibited a gradient evolution from the near-surface to the substrate.This evolution was characterized by an equiaxed nanocrystalline layer measuring 0.34μm,followed by a submicrocrystalline grain-forming zone spanning 0.6μm and finally,a constituent phase-twisted dis-torted deformation zone over 0.62μm.Under normal grinding conditions,the tenon exhibited low surface hardening(not exceeding 15%),and residual compressive stresses were observed on its surface.In cases where grinding burns occurred,a white layer appeared on the tenon's surface,which demonstrated varying thicknesses along the teeth from top to root due to thermal-force-structural coupling effects.Additionally,these burns introduced residual tensile stresses on the tenon's surface,potentially substantially affecting its fatigue life.This paper enhances our understanding of UVAG processes and establishes a foundation for their application in manufacturing singlecrystal turbine blades for next-generation aero-turbine engines. 展开更多
关键词 Single-crystal blade tenon Seeded gel abrasives ultrasonic vibration-assisted profile grinding Surface integrity
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Ultrasonic integrated backscatter in assessing liver steatosis before and after liver transplantation 被引量:1
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作者 Han-Teng Yang Ke-Fei Chen +4 位作者 Qiang Lu Yong-Gang Wei Bo Li Yang Qin Wen-Qing Huang 《Hepatobiliary & Pancreatic Diseases International》 SCIE CAS 2014年第4期402-408,共7页
BACKGROUND: Liver steatosis affects 20%-30% of adults.Because of the increasing gap between graft supplies and demands, livers with steatosis are frequently used in liver transplantation. But severely steatotic liver... BACKGROUND: Liver steatosis affects 20%-30% of adults.Because of the increasing gap between graft supplies and demands, livers with steatosis are frequently used in liver transplantation. But severely steatotic liver grafts are associated with a high risk of intraoperative and postoperative complications. Accurate assessment of fat content of donor livers and monitoring of the extent of steatosis in recipients are required for liver transplantation. The present study aimed to determine the correlation between liver echogenicity and fat content, and to evaluate the use of an ultrasonic integrated backscatter system(IBS) in the assessment of changes in fat content after liver transplantation.METHODS: Seventy-nine consecutive patients receiving liver grafts from living donors were evaluated in our center. Of these recipients, 67 survived for more than two years and were included in this study. Each liver graft was evaluated with IBS and ultrasound before operation and the fat content was estimated. The fat content of the grafts in the recipients was again assessed with ultrasound at 18 months after surgery.RESULTS: A correlation was detected between each graft's IBS value and its fat content(P=0.001). The IBS value in fatty grafts with various degrees of steatosis was significantly decreased in 3(P=0.02), 12, 15 and 18(P=0.001) months after orthotopic liver transplantation. The IBS value returned to normal in all patients in 18 months after liver transplantation.CONCLUSIONS: Decreased fat content in steatotic grafts can be observed in all recipients. Ultrasonic IBS is useful in determining the steatotic degree of grafts in donors as well as in monitoring the grafts after liver transplantation. 展开更多
关键词 fat content fatty liver liver transplantation ultrasonic integrated backscatter
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Noncontact Driving System Using Induction-Based Method and Integrated Piezoelectric Ultrasonic Transducers
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作者 Riichi Murayama Makiko Kobayashi +1 位作者 Kuo-Ting Wu Cheng-Kuei Jen 《Journal of Sensor Technology》 2012年第2期60-67,共8页
Integrated Ultrasonic Transducers (IUTs) have been developed for high-temperature nondestructive evaluation applications. In many field, it would be helpful if a pipe covered by a protective layer of about 10 cm thick... Integrated Ultrasonic Transducers (IUTs) have been developed for high-temperature nondestructive evaluation applications. In many field, it would be helpful if a pipe covered by a protective layer of about 10 cm thickness, which is under operation at several hundred Celsius, could be inspected from above the protective layer by an IUT. As a first step toward achieving the inspection of such a pipeline, an induction-based method using coils is presented together with IUTs. This study focuses on the effects of the separation distance (liftoff) between the coils on the ultrasonic signal strength and bandwidth of the IUTs. Ultrasonic signals were generated and received by the IUTs on a steel plate with a sufficient strength for thickness measurements when the liftoff was 20 cm. It was also shown that a ferrite disc together with the coils enhanced the received signal strength even when the liftoff was over 10 cm. 展开更多
关键词 ultrasonic Wave NONCONTACT Sensing integrated ultrasonic TRANSDUCER Electromagnetic INDUCTION LAW
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Ironing effect on surface integrity and fatigue behavior during ultrasonic peening drilling of Ti-6Al-4V 被引量:7
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作者 Yihang LIU Deyuan ZHANG +5 位作者 Daxi GENG Zhenyu SHAO Zehua ZHOU Zhefei SUN Yonggang JIANG Xinggang JIANG 《Chinese Journal of Aeronautics》 SCIE EI CAS CSCD 2023年第5期486-498,共13页
Imposing compressive residual stress field around a fastening hole serves as a universal method to enhance the hole fatigue strength in the aircraft assembly filed.Ultrasonic Peening Drilling(UPD)is a recently propose... Imposing compressive residual stress field around a fastening hole serves as a universal method to enhance the hole fatigue strength in the aircraft assembly filed.Ultrasonic Peening Drilling(UPD)is a recently proposed hybrid hole making process,which can achieve an integration of strengthening and precision-machining with a one-shot-drilling operation.Due to the ironing effect of tool flank surface,UPD introduces large compressive residual stress filed in hole subsurface.In order to reveal the strengthening mechanism of UPD,the influence of ultrasonic vibration and tool dynamic relief angle on ironing coverage rate and its corresponding effect on surface integrity in UPD were analyzed.The experiments were conducted to verify the influence of ironing effect on surface integrity and fatigue behavior of Ti-6Al-4V hole in UPD.The results indicate that the specimen features smaller surface roughness,higher micro-hardness,plastic deformation degree and circumferential compress residual stress under higher ironing coverage rate.The fatigue life increases with the raise of ironing coverage rate,and the fatigue source site in UPD shifts from surface to subsurface comparing with that without vibration assistance.The results demonstrates that a better strengthening effect can be obtained by reasonably controlling the ironing coverage rate in UPD. 展开更多
关键词 Fatigue strength Surface integrity Titanium alloy Tool relief angle ultrasonic Peening Drilling(UPD)
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Improving fatigue properties of normal direction ultrasonic vibration assisted face grinding Inconel 718 by regulating machined surface integrity 被引量:1
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作者 Nianwei Xu Renke Kang +4 位作者 Bi Zhang Yuan Zhang Chenxu Wang Yan Bao Zhigang Dong 《International Journal of Extreme Manufacturing》 SCIE EI CAS CSCD 2024年第3期458-475,共18页
Fatigue properties are crucial for critical aero-engine components in extreme serviceenvironments,which are significantly affected by surface integrity(SI)indexes(especially surface topography,residual stressσ_(res),... Fatigue properties are crucial for critical aero-engine components in extreme serviceenvironments,which are significantly affected by surface integrity(SI)indexes(especially surface topography,residual stressσ_(res),and microhardness)after machining processes.Normal-direction ultrasonic vibration-assisted face grinding(ND-UVAFG)has advantages in improving the machinability of Inconel 718,but there is a competitive relationship between higher compressiveσ_(res)and higher surface roughness R_(a)in affecting fatigue strength.The lack of a quantitative relationship between multiple SI indexes and fatigue strength makes theindeterminacy of a regulatory strategy for improving fatigue properties.In this work,a model of fatigue strength(σ_f)_(sur)considering multiple SI indexes was developed.Then,high-cycle fatigue tests were carried out on Inconel 718 samples with different SI characteristics,and the influence of ND-UVAFG process parameters on SI was analyzed.Based on SI indexes data,the(σ_f)_(sur)distribution in the grinding surface layer for ND-UVAFG Inconel 718 samples was determined using the developed model,and then the fatigue crack initiation(FCI)sites were furtherpredicted.The predicted FCI sites corresponded well with the experimental results,therebyverifying this model.A strategy for improving the fatigue life was proposed in this work,which was to transfer the fatigue source from the machined surface to the bulk material by controlling the SI indexes.Finally,a critical condition of SI indexes that FCI sites appeared on the surface or in bulk material was given by fitting the predicted results.According to the critical condition,an SI field where FCI sites appeared in the bulk material could be obtained.In this field,thefatigue life of Inconel 718 samples could be improved by approximately 140%. 展开更多
关键词 surface integrity fatigue strength Inconel 718 ultrasonic assisted grinding
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High-performance milling of Ti-6Al-4V through rotary ultrasonic elliptical milling with anticlockwise elliptical vibration
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作者 Lianxing LIU Xinggang JIANG +3 位作者 Enze YING Zhefei SUN Daxi GENG Deyuan ZHANG 《Journal of Zhejiang University-Science A(Applied Physics & Engineering)》 2025年第8期707-722,共16页
Ultrasonic elliptical vibration cutting(UEVC)with clockwise elliptical vibration has made notable achievements in precision machining;however,its critical cutting speed limits its application to low-speed machining ta... Ultrasonic elliptical vibration cutting(UEVC)with clockwise elliptical vibration has made notable achievements in precision machining;however,its critical cutting speed limits its application to low-speed machining tasks.Meanwhile,rotary ultrasonic elliptical machining(RUEM)with clockwise elliptical vibration has been validated as an effective high-speed cutting technology.Unfortunately,conventional RUEM leads to increased surface roughness.To address this issue and enhance machining quality,we propose a novel RUEM method employing an anticlockwise vibration direction,called anticlockwise rotary ultrasonic elliptical machining(ARUEM).The mechanisms of surface formation and subsurface strengthening for ARUEM are analyzed.Experimental validations were performed on Ti-6Al-4V alloy,revealing that ARUEM achieved substantially lower ridge heights and up to a 50%reduction in surface roughness compared to conventional RUEM.Additionally,relative to conventional milling,ARUEM resulted in up to 122.6%thicker plastic deformation layers,53.4%higher surface residual compressive stress,and 19.3%greater surface micro-hardness.This study showcases a promising method for high-performance milling of Ti-6Al-4V,offers new insights into RUEM by examining the influence of vibration direction,and enhances understanding of surface formation and subsurface strengthening in the ARUEM method. 展开更多
关键词 ultrasonic elliptical vibration cutting(UEVC) Vibration direction Rotary ultrasonic elliptical machining(RUEM) Surface formation mechanism Surface integrity High-speed milling
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Influence of transversal vibration on cutting performance and surface integrity during ultrasonic peening drilling of Al-Li alloys
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作者 Zhefei SUN Daxi GENG +6 位作者 Hailin GUO Ende GE Entao ZHOU Zhilei FAN Fanxing MENG Xinggang JIANG Deyuan ZHANG 《Chinese Journal of Aeronautics》 SCIE EI CAS CSCD 2024年第10期493-507,共15页
Advanced hole-making process is of great importance to enhance the fatigue performance of Al-Li alloy part in aviation industry.Ultrasonic peening drilling(UPD),in which an ultrasonic transversal vibration is applied ... Advanced hole-making process is of great importance to enhance the fatigue performance of Al-Li alloy part in aviation industry.Ultrasonic peening drilling(UPD),in which an ultrasonic transversal vibration is applied to the cutting tools,is a recently proposed hole-making method that integrates precision-machining and surface strengthening by single-shot operation.In the study,kinematics,material removal mechanism and strengthening mechanism for UPD of Al-Li alloy by helical fluted reamers are analyzed.The effect of transversal vibration on the cutting performance and surface integrity is studied through comparative experiments between UPD and conventional drilling(CD)of Al-Li alloy holes.The experimental results show that UPD exhibits superior cutting performance with a maximum reduction of 52.6%in thrust force and 52.3%in torque,respectively,compared to CD.Moreover,narrower dimensional tolerance is obtained in UPD due to the reduced transversal force and improved machining stability.Additionally,deeper plastic deformation,higher surface microhardness and residual compressive stress of machined holes are obtained by UPD.The electron back-scattered diffraction(EBSD)analysis confirms that deeper machined affect area and grain refinement are realized in UPD.Therefore,the results indicate that UPD is a feasible method for achieving high-precision and strengthened holes for Al-Li alloy. 展开更多
关键词 Al-Li alloy ultrasonic transversal vibration Cutting tools Strengthening mechanism Surface integrity
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CONTROL OF ROBOT DRIVEN BY MULTIPLE ULTRASONIC MOTORS BASED ON ROBUST PARAMETER DESIGN 被引量:3
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作者 孙志峻 帅双辉 黄卫清 《Transactions of Nanjing University of Aeronautics and Astronautics》 EI 2009年第4期243-250,共8页
An ultrasonic motor (USM) is difficlt to be mathematically described because of its complex energy conversion and nonlinear parameters from increasing temperature and changing operating conditions. To achieve good p... An ultrasonic motor (USM) is difficlt to be mathematically described because of its complex energy conversion and nonlinear parameters from increasing temperature and changing operating conditions. To achieve good performance of a three-joint robot directly driven by USM, according to the operating characteristics of USM, a new position-velocity feedback control strategy is proposed. In the control strategy, there are a total of 18 controller gains to he tuned. Through a series of "Design of Experiments" by the robust parameter design, an optimal and robust set of proportional integral derivative (PID) controller gains is obtained. Results show that the control strategy can achieve the best performance of the robot and the robust parameter design is effective and convenient to USMs. 展开更多
关键词 ultrasonic transducers robust control proportional integral derivative (PID) control position-veloci-ty feedback
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Risk Indicator Identification for Coronary Heart Disease via Multi-Angle Integrated Measurements and Sequential Backward Selection
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作者 Hui Qi Jingyi Lian Congjun Rao 《Computer Modeling in Engineering & Sciences》 2025年第10期995-1028,共34页
For the past few years,the prevalence of cardiovascular disease has been showing a year-on-year increase,with a death rate of 2/5.Coronary heart disease(CHD)rates have increased 41%since 1990,which is the number one d... For the past few years,the prevalence of cardiovascular disease has been showing a year-on-year increase,with a death rate of 2/5.Coronary heart disease(CHD)rates have increased 41%since 1990,which is the number one disease endangering human health in the world today.The risk indicators of CHD are complicated,so selecting effective methods to screen the risk characteristics can make the risk predictionmore efficient.In this paper,we present a comprehensive analysis ofCHDrisk indicators fromboth data and algorithmic levels,propose a method for CHDrisk indicator identification based on multi-angle integrated measurements and Sequential Backward Selection(SBS),and then build a risk prediction model.In the multi-angle integrated measurements stage,mRMR(Maximum Relevance Minimum Redundancy)is selected from the angle of feature correlation and redundancy of the dataset itself,SHAPRF(SHapley Additive exPlanations-Random Forest)is selected from the angle of interpretation of each feature to the results,and ARFS-RF(Algorithmic Randomness Feature Selection Random Forest)is selected from the angle of statistical interpretation of classification algorithm to measure the degree of feature importance.In the SBS stage,the features with low scores are deleted successively,and the accuracy of LightGBM(Light Gradient Boosting Machine)model is used as the evaluation index to select the final feature subset.This new risk assessment method is used to identify important factors affecting CHD,and the CHD dataset from the Kaggle website is used as the study subject.Finally,11 features are retained to construct a risk assessment indicator system for CHD.Using the LightGBM classifier as the core evaluationmetric,ourmethod achieved an accuracy of 0.8656 on the Kaggle CHD dataset(4238 samples,16 initial features),outperforming individual feature selection methods(mRMR,SHAP-RF,ARFS-RF)in both accuracy and feature reduction.This demonstrates the novelty and effectiveness of our multi-angle integrated measurement approach combined with SBS in building a concise yet highly predictive CHD risk model. 展开更多
关键词 CHD multi-angle integrated measurements SBS LightGBM
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Active and passive compliant force control of ultrasonic surface rolling process on a curved surface 被引量:2
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作者 Kaiming ZHANG Ji WANG +2 位作者 Yixin LIU Shuang LIU Xiancheng ZHANG 《Chinese Journal of Aeronautics》 SCIE EI CAS CSCD 2022年第7期289-299,共11页
Ultrasonic surface rolling process(USRP)is one of the effective mechanical surface enhancement techniques.During the USRP,unstable static force will easily do harm to the surface quality.In order to achieve a higher s... Ultrasonic surface rolling process(USRP)is one of the effective mechanical surface enhancement techniques.During the USRP,unstable static force will easily do harm to the surface quality.In order to achieve a higher surface quality on the part with a curved surface,an active and passive compliant USRP system has been developed.The compliant USRP tool can produce the natural obedience deformation along the part surface.Force control based on the fuzzy Proportional-integral-derivative(PID)method is then designed to maintain the static force during the USRP.Experiments have been performed on a real aero-engine blade with curved surface.It is proved that the deigned active and passive compliant USRP system can significantly reduce the force variation from 42.2 N to 4.2 N,and achieve a uniform surface quality after processing. 展开更多
关键词 Active and passive compliant Curved surface Force control Surface integrity ultrasonic surface rolling process
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Development of an Integrated CMUTs-Based Resonant Biosensor for Label-Free Detection of DNA with Improved Selectivity by Ethylene-Glycol Alkanethiols
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作者 Zhikang Li Yihe Zhao +7 位作者 Gian Luca Barbruni Jie Li Zixuan Li Jiawei Yuan Ping Yang Libo Zhao Zhuangde Jiang Sandro Carrara 《Engineering》 SCIE EI CAS CSCD 2024年第10期231-241,共11页
Gravimetric resonant-inspired biosensors have attracted increasing attention in industrial and point-ofcare applications,enabling label-free detection of biomarkers such as DNA and antibodies.Capacitive micromachined ... Gravimetric resonant-inspired biosensors have attracted increasing attention in industrial and point-ofcare applications,enabling label-free detection of biomarkers such as DNA and antibodies.Capacitive micromachined ultrasonic transducers(CMUTs)are promising tools for developing miniaturized highperformance biosensing complementary metal–oxide–silicon(CMOS)platforms.However,their operability is limited by inefficient functionalization,aggregation,crosstalk in the buffer,and the requirement for an external high-voltage(HV)power supply.In this study,we aimed to propose a CMUTs-based resonant biosensor integrated with a CMOS front–end interface coupled with ethylene–glycol alkanethiols to detect single-stranded DNA oligonucleotides with large specificity.The topography of the functionalized surface was characterized by energy-dispersive X-ray microanalysis.Improved selectivity for onchip hybridization was demonstrated by comparing complementary and non-complementary singlestranded DNA oligonucleotides using fluorescence imaging technology.The sensor array was further characterized using a five-element lumped equivalent model.The 4 mm^(2) application-specific integrated circuit chip was designed and developed through 0.18 lm HV bipolar-CMOS-double diffused metal–oxide–silicon(DMOS)technology(BCD)to generate on-chip 20 V HV boosting and to track feedback frequency under a standard 1.8 V supply,with a total power consumption of 3.8 mW in a continuous mode.The measured results indicated a detection sensitivity of 7.943×10^(-3) lmol·L^(-1)·Hz^(-1) over a concentration range of 1 to 100 lmol·L^(-1).In conclusion,the label-free biosensing of DNA under dry conditions was successfully demonstrated using a microfabricated CMUT array with a 2 MHz frequency on CMOS electronics with an internal HV supplier.Moreover,ethylene–glycol alkanethiols successfully deposited self-assembled monolayers on aluminum electrodes,which has never been attempted thus far on CMUTs,to enhance the selectivity of bio-functionalization.The findings of this study indicate the possibility of full-on-chip DNA biosensing with CMUTs. 展开更多
关键词 Capacitive micromachined ultrasonic transducers(CMUTs) DNA detection Self-assembled monolayer(SAM) Ethylene-glycol alkanethiols Application-specific integrated circuit(ASIC)
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基于复合算法的超声电源频率跟踪设计
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作者 潘行舟 陈涛 +1 位作者 段振岩 潘鑫龙 《应用声学》 北大核心 2026年第1期46-53,共8页
为解决在超声辅助加工中超声电源由负载、温度等因素变化导致的谐振频率漂移、输出功率不稳定等问题,设计了一种可实现频率跟踪的超声电源。以STM32单片机作为主控系统,基于直接数字式频率合成器技术并利用全桥逆变电路进行频率输出,采... 为解决在超声辅助加工中超声电源由负载、温度等因素变化导致的谐振频率漂移、输出功率不稳定等问题,设计了一种可实现频率跟踪的超声电源。以STM32单片机作为主控系统,基于直接数字式频率合成器技术并利用全桥逆变电路进行频率输出,采用遗传算法与模糊控制器对比例积分微分(PID)控制进行优化,将电压和电流相位差信号作为PID控制输入。仿真和实验结果表明,当负载参数发生改变时,电源的谐振频率发生漂移,经过频率自动跟踪子系统的调节后,电源快速回到谐振状态。此控制算法使得超声电源输出频率能够稳定在换能器谐振频率附近,有助于超声辅助加工的稳定工作。 展开更多
关键词 超声电源 遗传算法 模糊控制 比例积分微分 频率跟踪
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超声多角度组合果树冠层体积探测系统的设计与试验
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作者 刘雨鑫 张云龙 +4 位作者 吴建国 郑康 杨硕 秦向阳 王秀 《农机化研究》 北大核心 2026年第4期197-205,共9页
针对目前我国果树冠层体积在线探测系统实际使用价格高、适应性差、难以满足精准施药需求的问题,本研究提出了一种基于超声波传感器的冠层体积快速探测方法。通过结合树冠高度调整超声波发射角度,获取不同高度下果树冠体直径;采用椭圆... 针对目前我国果树冠层体积在线探测系统实际使用价格高、适应性差、难以满足精准施药需求的问题,本研究提出了一种基于超声波传感器的冠层体积快速探测方法。通过结合树冠高度调整超声波发射角度,获取不同高度下果树冠体直径;采用椭圆法、网格法获取对应高度截面积,通过分层体积累加方法快速获得冠层体积。试验使用4款国产超声传感器对不同直径的4个PVC靶标进行精准探测,并开展了特性分析,结果表明4种传感器都具有较好的线性探测性能。其中,超声传感器UBX400-GM30-D1-H5型号在50 mm靶标检测中较UM4000-SR55-AI-VC型号性能提升33%,可以实现冠层覆盖情况下的精准测定。开展了0.2、0.8、1.0、1.2、1.4 m/s等5个车速下的果树冠层体积探测精度田间试验,结果表明:网格法和椭圆法冠层体积探测平均误差分别为(4.14±0.01)%和(5.77±0.02)%;随着采样速度的增加,探测精度没有显著差异,设计的冠层体积探测系统具有较好的稳定性和冠层体积探测精度。本研究为果树对靶精准施药低成本推广提供了技术支撑。 展开更多
关键词 果树冠层体积 生物量探测 超声多角度组合 对靶施药
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新型超声快速处理活检标本保存不同年限对DNA质量的影响
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作者 石晨曦 朱卫东 +3 位作者 李三恩 李秀明 师逢 丁亚云 《中国组织工程研究》 CAS 北大核心 2025年第13期2655-2660,共6页
背景:新型超声组织处理技术越来越多地被用来进行分子生物学分析,研究新型超声处理不同存储年限组织DNA的质量,对进一步分子检测的标本质控具有重要意义。目的:探讨新型超声处理活检标本存储不同年限对DNA质量的影响,以期为分子检测探... 背景:新型超声组织处理技术越来越多地被用来进行分子生物学分析,研究新型超声处理不同存储年限组织DNA的质量,对进一步分子检测的标本质控具有重要意义。目的:探讨新型超声处理活检标本存储不同年限对DNA质量的影响,以期为分子检测探索最佳的标本存储时间。方法:收集40例乳腺穿刺小活检组织,采用超声技术制作石蜡标本,按照存储年限分为4组:<1年组、1-3年组、>3-5年组及>5年组,每组10例,对石蜡标本进行切片,每张切片厚3μm,切片10-15张,提取DNA后通过Nanophotometer N60超微量分光光度计和Qubit 4.0荧光计检测DNA的质量浓度,记录A_(260)/A_(280)比值判定DNA的纯度,利用全自动毛细管电泳核酸分析仪(Qsep 100)检测DNA片段完整性,以评估DNA片段的质量。结果与结论:4组样本A_(260)/A_(280)均值在1.8-2.0之间,达到纯度要求,无明显差异。4组样本的DNA质量浓度(Qubit浓度)均值分别为30.39,14.33,2.52,1.95 ng/μL;DNA的平均N/Q比值分别为6.48,14.18,24.56,29.86;DNA质量数均值分别为5.64,1.76,1.24,0.80;大片段占比均值分别为56.08%,17.72%,12.68%,7.90%。PCR检测内控基因Ct均值分别为15.32,17.09,18.39,21.24。与<1年组相比,其余3组DNA浓度显著降低,N/Q比值显著增加,DNA质量数和大片段占比均值显著降低,Ct值升高,差异有显著性意义(P<0.05)。实验结果表明,对于新型超声处理活检标本,应优先选择存储<1年的样本进行日常分子检测,储存3年内的样本可满足二代测序等检测要求,5年内样本仅可尝试进行PCR等检测,存储超过5年的样本不建议进行后续分子检测。 展开更多
关键词 超声处理 存储年限 DNA质量 片段完整性 降解程度 二代测序
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超声滚压对AISI420不锈钢旋转弯曲疲劳性能的影响
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作者 程俊 许志龙 +2 位作者 弓清忠 李波 翁建春 《塑性工程学报》 北大核心 2025年第4期82-89,共8页
采用不同的工艺参数组合对AISI420不锈钢试样进行超声滚压处理,研究超声滚压工艺对其旋转弯曲疲劳性能的影响。使用激光共聚焦显微镜、扫描电镜和维氏硬度计等设备对超声滚压处理前后试样的表面完整性进行了表征,包括表面形貌、表面粗... 采用不同的工艺参数组合对AISI420不锈钢试样进行超声滚压处理,研究超声滚压工艺对其旋转弯曲疲劳性能的影响。使用激光共聚焦显微镜、扫描电镜和维氏硬度计等设备对超声滚压处理前后试样的表面完整性进行了表征,包括表面形貌、表面粗糙度、表层硬度和残余应力分布等。结果表明,随着超声滚压静压力的增加,表面粗糙度先大幅降低,后小幅上升,表面粗糙度Ra最低达到0.19μm;表层硬度随着静压力的增加而增加,硬度影响层的深度约200μm;超声滚压在表层引入梯度分布的残余压应力,表层最大残余压应力达到462 MPa。随着静压力的增加,残余压应力沿深度方向先增加后减小。超声滚压处理后,AISI420不锈钢试样的旋转弯曲疲劳寿命比未处理试样最大提高了约2.8倍。分析了表面完整性对疲劳寿命的影响,结果显示表层残余压应力是提高疲劳寿命的关键因素。 展开更多
关键词 超声滚压 AISI420不锈钢 表面完整性 疲劳性能
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超声滚压静压力对20CrNiMo钢表面完整性及疲劳性能的影响
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作者 程俊 李毅 李波 《机械工程材料》 北大核心 2025年第6期76-82,共7页
采用超声滚压工艺对渗碳+淬回火态20CrNiMo钢试样进行表面强化处理,研究了不同静压力(700,800,900,1000 N)下试样的表面粗糙度、硬度、残余压应力分布以及旋转弯曲疲劳性能。结果表明:超声滚压后,试样的表面粗糙度明显降低,且随着静压... 采用超声滚压工艺对渗碳+淬回火态20CrNiMo钢试样进行表面强化处理,研究了不同静压力(700,800,900,1000 N)下试样的表面粗糙度、硬度、残余压应力分布以及旋转弯曲疲劳性能。结果表明:超声滚压后,试样的表面粗糙度明显降低,且随着静压力的增加呈先降低后增加的趋势,当静压力为900 N时,表面粗糙度R_(a)最低,为0.17μm。超声滚压后,试样的硬度提高,且随着静压力的增加呈升高趋势。超声滚压在试样表层引入梯度分布的残余压应力;随着静压力的增加,残余压应力最大值先增后降,在静压力为900 N时达到最大,为1128 MPa。超声滚压后试样的旋转弯曲疲劳寿命显著提高,且随着静压力的增加先增后降。当静压力为900 N时,疲劳寿命最高,达到697.3×10^(4)周次,此时疲劳裂纹源为单裂纹源,位于次表层。试样表层残余压应力最大值的变化趋势与疲劳寿命具有高度相关性,是影响疲劳寿命的关键因素。 展开更多
关键词 超声滚压 20CrNiMo钢 表面完整性 疲劳性能 残余应力
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集成化寻址超声传感器阵列接收电路设计及应用
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作者 宋寿鹏 毛新宇 《东南大学学报(自然科学版)》 北大核心 2025年第6期1798-1807,共10页
寻址超声传感器阵列具有灵活选择发射和接收阵元的优势,但需配合相应的电路才能实现其功能。针对该型阵列接收电路相对匮乏的现状,提出了一种可用于64阵元寻址阵列的集成化电路设计方案,在实现灵活选通接收阵元的同时,降低电路系统的复... 寻址超声传感器阵列具有灵活选择发射和接收阵元的优势,但需配合相应的电路才能实现其功能。针对该型阵列接收电路相对匮乏的现状,提出了一种可用于64阵元寻址阵列的集成化电路设计方案,在实现灵活选通接收阵元的同时,降低电路系统的复杂度和体积。采用8通道集成化模拟前端芯片电路,实现8+8寻址超声传感器阵列的行单元选通接收机制。该接收电路系统采用FPGA进行阵元选通时序和数据传输控制,以提高时序控制精度和数据传输的实时性与准确性。制备了寻址激励电路和集成化接收电路系统,利用8+8寻址超声传感器阵列测试铝试块中孔类缺陷,并进行缺陷全聚焦成像性能分析。试验结果表明,在选通单阵元、行阵元和列阵元的收发模式下,Ф4 mm通孔缺陷的回波信噪比分别为19.77、21.45和21.28 dB,缺陷深度估计相对误差分别为0.61%、1.17%和1.83%,去除伪影后的缺陷图像阵列性能参数分别为0.403 1、0.286 5和0.370 9。 展开更多
关键词 超声检测 传感器阵列 寻址接收 集成化电路
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基于回波波形积分的气体超声流量计信号处理方法
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作者 陈洁 杨浩男 李斌 《计量学报》 北大核心 2025年第11期1598-1605,共8页
针对气体超声波流量计信号处理中难以找到稳定的测量特征点的问题,通过模型推导和实流验证,研究构建了一种基于回波波形积分的信号处理方法。该方法利用回波波形上半周期的各采样点求取回波波形能量,通过求取采样点累积能量找到最接近... 针对气体超声波流量计信号处理中难以找到稳定的测量特征点的问题,通过模型推导和实流验证,研究构建了一种基于回波波形积分的信号处理方法。该方法利用回波波形上半周期的各采样点求取回波波形能量,通过求取采样点累积能量找到最接近阈值的2个采样点,得到特征点,从而计算超声波渡越时间及气体流量。在基于STM32的双核心系统上实时完成该信号处理方法,并在国家认可的检测机构浙江迪元仪表有限公司进行标定实验,结果表明:基于回波波形积分的信号处理方法的超声波流量计达到1.0级准确度。 展开更多
关键词 流量计量 气体超声波流量计 信号处理 回波波形积分 数学模型 测量特征点
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表面复合强化对18CrNiMo7-6渗碳齿轮钢表面完整性的影响研究
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作者 王振 孙浩 +3 位作者 崔义龙 李林艳 王涛 韩家宝 《机械强度》 北大核心 2025年第8期36-47,共12页
喷丸强化工艺广泛应用于齿轮等基础零部件的制造过程,其自身局限性限制了工件表面完整性的提升。为进一步提升工件表面完整性,采用数值模拟与试验相结合的方法,研究二次喷丸、喷丸-超声滚压这2种表面复合强化工艺对18CrNiMo7-6渗碳齿轮... 喷丸强化工艺广泛应用于齿轮等基础零部件的制造过程,其自身局限性限制了工件表面完整性的提升。为进一步提升工件表面完整性,采用数值模拟与试验相结合的方法,研究二次喷丸、喷丸-超声滚压这2种表面复合强化工艺对18CrNiMo7-6渗碳齿轮钢试样表面完整性的影响效果,主要分析两种复合强化工艺对喷丸强化试样表面完整性的改善效果。结果表明,二次喷丸强化试样残余压应力最大值为1359.56 MPa,位于0.08 mm的深度位置。喷丸-超声滚压强化试样残余压应力最大值为1329.05 MPa,位于0.25 mm的深度位置。相较于单次喷丸强化试样,二次喷丸强化试样和喷丸-超声滚压强化试样,表面粗糙度分别下降29.42%、62.76%,表面显微硬度分别提升8.70%、17.60%,表面节点残余压应力值标准差分别下降23.36%、89.50%。喷丸-超声滚压强化工艺在提升试样表面硬度、残余应力层厚度和残余压应力均匀性以及降低表面粗糙度等方面效果更显著,能够有效改善喷丸强化试样的表面完整性。 展开更多
关键词 18CrNiMo7‑6钢 喷丸 超声滚压 表面完整性
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超声兰姆波扫描成像测井仪器研制及应用
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作者 吴金平 《地球物理学进展》 北大核心 2025年第5期2357-2364,共8页
检测水泥胶结质量和套管腐蚀损伤状况是保持井筒完整性的重要举措,然而,井筒完整性测井面临周向分辨率差、测量精度低、轻质水泥评价效果差等挑战.为了解决这些问题,提出了超声波垂直入射与斜入射组合测量设计方案,研制了超声兰姆波扫... 检测水泥胶结质量和套管腐蚀损伤状况是保持井筒完整性的重要举措,然而,井筒完整性测井面临周向分辨率差、测量精度低、轻质水泥评价效果差等挑战.为了解决这些问题,提出了超声波垂直入射与斜入射组合测量设计方案,研制了超声兰姆波扫描成像测井仪器.仪器集成了旋转扫描声系总成、滚轮弹性扶正器、传动机构及测量传感器总成和电子仓短节等部件,采用井下存储为主、电缆传输为辅的快速测井作业模式,并以高分辨率图像直观显示水泥胶结质量和套管腐蚀损伤程度.现场应用表明,仪器全方位360°扫描测量完全覆盖整个井筒,方位成像图直观指示水泥缺少、窜槽、套管腐蚀等周向位置,在轻质水泥固井质量评价具有显著优势,为油气井安全稳定生产提供了技术保障. 展开更多
关键词 超声兰姆波 套管井测井 水泥胶结质量 套管损伤 井筒完整性
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