In this manuscript,we first report an ultrasensitive detection assay of microRNA by combing asymmetric polymerase chain reaction(A-PCR)and loop-mediated isothermal amplification(LAMP)technology.Using A-PCR obtained an...In this manuscript,we first report an ultrasensitive detection assay of microRNA by combing asymmetric polymerase chain reaction(A-PCR)and loop-mediated isothermal amplification(LAMP)technology.Using A-PCR obtained an extended single strand to form LAMP stem-loop structure under isothermal amplification conditions.We used miRNAs as a loop primer probe in LAMP reaction and completed its ultrasensitive and rapid detection.The established method furnished a fast,specific and efficient detection of target miRNA with a detection limit as low as 10 amol/L in 90 min.展开更多
Two-dimensional covalent organic framework(COF)has distinctive properties that offer potential opportunities for developing advanced electrode materials.In this work,a core-shell material composed of TAPB-DMTP-COF(TAP...Two-dimensional covalent organic framework(COF)has distinctive properties that offer potential opportunities for developing advanced electrode materials.In this work,a core-shell material composed of TAPB-DMTP-COF(TAPB,1,3,5-tris(4-aminophenyl)benzene;DMTP,2,5-dimethoxyterephaldehyde)core and conducting polymer shell,TAPB-DMTP-COF@PANI,was synthesized solvothermally using a polymerization method.The structural cha racteristics of the prepared composite were revealed by X-ray diffraction patterns(XRD),fourier transform infrared spectra(FTIR),X-ray photoelectron spectroscopy(XPS),transmission electron microscopy(TEM).The electrochemical analyses were verified by subsequent monitoring of trace levels of acetaminophen.This resultant composite not only facilitated acetaminophen to interact with absorption sites byπ-πstacking effect and hydrogen bonding but also overcame the poor conductivity of COF.Under the optimal conditions,a low limit of detection of 0.032μmol/L and wide linear range of 0.10-500μmol/L were obtained.The electrochemical platform was almost unaffected by other interfering substances,and successfully applied for the practical detection of acetaminophen in commercial tablet,human blood serum and urine.The enhanced performance makes this COF based core-shell composite a promising material in electrochemical senso r.展开更多
Nowadays,high-stable and ultrasensitive heavy metal detection is of utmost importance in water quality monitoring.Nanoparticle-enhanced laser-induced breakdown spectroscopy(NELIBS)shows high potential in hazardous met...Nowadays,high-stable and ultrasensitive heavy metal detection is of utmost importance in water quality monitoring.Nanoparticle-enhanced laser-induced breakdown spectroscopy(NELIBS)shows high potential in hazardous metal detection,however,encounters unstable and weak signals due to nonuniform distribution of analytes.Herein,we developed an interface self-assembly(ISA)method to create a uniformly distributed gold nanolayer at a liquid-liquid interface for positive heavy metal ions capture and NELIBS analysis.The electrostatically selfassembled Au nanoparticles(NPs)-analytes membrane was prepared at the oil-water interface by injecting ethanol into the mixture of cyclohexane and Au NPs-analytes water solution.Then,the interface self-assembled Au NPs-analytes membrane was transformed onto a laser-processed superhydrophilic Si slide for detection.Three heavy metals(cadmium(Cd),barium(Ba),and chromium(Cr))were analyzed to evaluate the stability and sensitivity of the ISA method for NELIBS.The results(Cd:RSD=3.6%,LoD=0.654 mg/L;Ba:RSD=3.4%,LoD=0.236 mg/L;Cr:RSD=7.7%,LoD=1.367 mg/L)demonstrated signal enhancement and high-stable and ultrasensitive detection.The actual sample detection(Cd:RE=7.71%,Ba:RE=6.78%)illustrated great reliability.The ISA method,creating a uniform distribution of NP-analytes at the interface,has promising prospects in NELIBS.展开更多
Gastric cancer(GC)is a prevalent malignant tumor within the digestive system,with over 40%of new cases and deaths related to GC globally occurring in China.Despite advancements in treatment modalities,such as surgery ...Gastric cancer(GC)is a prevalent malignant tumor within the digestive system,with over 40%of new cases and deaths related to GC globally occurring in China.Despite advancements in treatment modalities,such as surgery supplemented by adjuvant radiotherapy or chemotherapeutic agents,the prognosis for GC remains poor.New targeted therapies and immunotherapies are currently under invest-igation,but no significant breakthroughs have been achieved.Studies have indicated that GC is a heterogeneous disease,encompassing multiple subtypes with distinct biological characteristics and roles.Consequently,personalized treatment based on clinical features,pathologic typing,and molecular typing is crucial for the diagnosis and management of precancerous lesions of gastric cancer(PLGC).Current research has categorized GC into four subtypes:Epstein-Barr virus-positive,microsatellite instability,genome stability,and chromosome instability(CIN).Technologies such as multi-omics analysis and gene sequencing are being employed to identify more suitable novel testing methods in these areas.Among these,ultrasensitive chromosomal aneuploidy detection(UCAD)can detect CIN at a genome-wide level in subjects using low-depth whole genome sequencing technology,in conjunction with bioinformatics analysis,to achieve qualitative and quantitative detection of chromosomal stability.This editorial reviews recent research advancements in UCAD technology for the diagnosis and management of PLGC.展开更多
The fabrication of sensitive sensors with high selectivity is highly desirable for the detection of some important biomarkers,such as nucleic acids,proteins,small molecules and ions.DNA hybridization chain reaction(HC...The fabrication of sensitive sensors with high selectivity is highly desirable for the detection of some important biomarkers,such as nucleic acids,proteins,small molecules and ions.DNA hybridization chain reaction(HCR) and DNA supersandwich self-assembly(SSA) are two prevalent enzyme-free signal amplification strategies to improve sensitivity of the sensors.In this review,we firstly describe the characteristics about DNA HCR and DNA SSA,and then summarize the advances in the one-dimensional DNA nanostructures assisted by HCR and SSA.This review has been divided into three parts according to the two signal amplification methods and highlights recent progress in these two strategies to improve the detection sensitivity of proteins,nucleic acids,small molecules and ions.展开更多
The simultaneous detection of carcinoembryonic antigen(CEA)and microRNA-21(miR-21)is of significant clinical importance for cancer diagnosis,prognosis evaluation,and therapy monitoring.In this study,we developed a nov...The simultaneous detection of carcinoembryonic antigen(CEA)and microRNA-21(miR-21)is of significant clinical importance for cancer diagnosis,prognosis evaluation,and therapy monitoring.In this study,we developed a novel electrochemical biosensor utilizing a peptide-self-assembly-engineered signal amplification(PSA-e-SA)nanoarchitectonic strategy to achieve ultrasensitive and simultaneous quantification of these two critical biomarkers.By designing amphiphilic peptides(C_(16)-MB-AptamerCEA and C_(16)-Fc-ssDNA2)as multifunctional probes,the system exploits their dual roles in target recognition and signal amplification.These peptides self-assemble into nanostructures under mild conditions,facilitating enhanced loading of electroactive molecules such as methylene blue(MB)and ferrocene(Fc),thereby significantly amplifying the electrochemical signal.The biosensor achieved detection limits of 0.788 pM for CEA and 0.0357 nM for miR-21,demonstrating remarkable sensitivity enhancements of 18-fold and 3.8-fold compared to unamplified approaches.As a proof-of-concept study,further experiments underscored the excellent reproducibility and stability of the strategy while also demonstrating its applicability when tested with simulated serum samples.Thus,this work not only presents a valuable assay tool for early cancer diagnosis and biomarker analysis but also indicates that this signal amplification strategy based on peptide self-assembly engineering can be extended to detect other disease-related biomarkers,propelling the development of clinical applications for multifunctional biosensors.展开更多
Exceptional point(EP)is referred to degeneracies in a non-Hermitian system where two or more eigenvalues and their corresponding eigenvectors coalesce.Recently there have been significantly increased interests in harn...Exceptional point(EP)is referred to degeneracies in a non-Hermitian system where two or more eigenvalues and their corresponding eigenvectors coalesce.Recently there have been significantly increased interests in harnessing EPs to enhance responsivities and achieve ultrasensitive detections in optics,electronics and acoustics,although there are few similar studies focused on using surface acoustic wave(SAW)sensing technologies,probably due to its great technical challenges.Herein,we proposed a scheme for accessing EPs in an on-chip architecture consisted of coupledSAW-resonators system,forming a passive parity-time(PT)symmetric system.We demonstrated that by tuning additional losses in one of resonators and regulating the system in the proximity of the EP,the sensor exhibited significantly enhanced responses.As an example,we present an EP-based SAW gas sensor,which showed a muchimproved sensitivity compared to that of a conventional delay-line SAW sensor.The fundamental mechanisms behind this excellent sensing performance have been elucidated.展开更多
Circular dichroism(CD)spectroscopy,widely used for chiral sensing,has been limited by the detection sensitivity.Enhancing optical chirality in the light fields interacting with chiral molecules is crucial for achievin...Circular dichroism(CD)spectroscopy,widely used for chiral sensing,has been limited by the detection sensitivity.Enhancing optical chirality in the light fields interacting with chiral molecules is crucial for achieving ultrasensitive chiral detection.Here,we present a new paradigm for ultrasensitive chiral detection by creating accessible chiral hotspots using a toroidal dipole Fabry–Perot bound state in the continuum(TD FP-BIC)metasurface.BIC resonance is achieved by controlling the coupling between the TD resonance and its multilayer reflector-induced perfect mirror image.This method enables unprecedented local maximum and average optical chirality enhancements of up to 6×10^(4)-fold and 2×10^(3)-fold,respectively,within non-structured regions,resulting in an 866-fold increase in CD signals compared to chiral molecules alone without nanostructures.Our results pave the way for enhanced light–matter interactions and ultrasensitive enantiomeric operation.展开更多
基金supported by the National Key R&D Program of China (Nos.2017YFA0208100,2016YFA0602900)National Natural Science Foundation of China (Nos.91853124,21778057 and 21420102003)Chinese Academy of Sciences
文摘In this manuscript,we first report an ultrasensitive detection assay of microRNA by combing asymmetric polymerase chain reaction(A-PCR)and loop-mediated isothermal amplification(LAMP)technology.Using A-PCR obtained an extended single strand to form LAMP stem-loop structure under isothermal amplification conditions.We used miRNAs as a loop primer probe in LAMP reaction and completed its ultrasensitive and rapid detection.The established method furnished a fast,specific and efficient detection of target miRNA with a detection limit as low as 10 amol/L in 90 min.
基金supported by the National Natural Science Foundation of China(No.21205103)Jiangsu Provincial Natural Science Foundation(No.BK2012258)+2 种基金Young and Middle-aged Academic Leaders Foundation of Yangzhou UniversityTop-notch Academic Programs Project of Jiangsu Higher Education Institutions(TAPP)funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD)。
文摘Two-dimensional covalent organic framework(COF)has distinctive properties that offer potential opportunities for developing advanced electrode materials.In this work,a core-shell material composed of TAPB-DMTP-COF(TAPB,1,3,5-tris(4-aminophenyl)benzene;DMTP,2,5-dimethoxyterephaldehyde)core and conducting polymer shell,TAPB-DMTP-COF@PANI,was synthesized solvothermally using a polymerization method.The structural cha racteristics of the prepared composite were revealed by X-ray diffraction patterns(XRD),fourier transform infrared spectra(FTIR),X-ray photoelectron spectroscopy(XPS),transmission electron microscopy(TEM).The electrochemical analyses were verified by subsequent monitoring of trace levels of acetaminophen.This resultant composite not only facilitated acetaminophen to interact with absorption sites byπ-πstacking effect and hydrogen bonding but also overcame the poor conductivity of COF.Under the optimal conditions,a low limit of detection of 0.032μmol/L and wide linear range of 0.10-500μmol/L were obtained.The electrochemical platform was almost unaffected by other interfering substances,and successfully applied for the practical detection of acetaminophen in commercial tablet,human blood serum and urine.The enhanced performance makes this COF based core-shell composite a promising material in electrochemical senso r.
基金supported by the National Natural Science Foundation of China(No.62075069 and 52303092)the Water Conservancy Technology project of Hunan Province,China(XSKJ2021000-32)+1 种基金the City University of Hong Kong(#7005507)the Open Project of Yunnan Precious Metals Laboratory Co.,Ltd(grant number YPML-2023050278).
文摘Nowadays,high-stable and ultrasensitive heavy metal detection is of utmost importance in water quality monitoring.Nanoparticle-enhanced laser-induced breakdown spectroscopy(NELIBS)shows high potential in hazardous metal detection,however,encounters unstable and weak signals due to nonuniform distribution of analytes.Herein,we developed an interface self-assembly(ISA)method to create a uniformly distributed gold nanolayer at a liquid-liquid interface for positive heavy metal ions capture and NELIBS analysis.The electrostatically selfassembled Au nanoparticles(NPs)-analytes membrane was prepared at the oil-water interface by injecting ethanol into the mixture of cyclohexane and Au NPs-analytes water solution.Then,the interface self-assembled Au NPs-analytes membrane was transformed onto a laser-processed superhydrophilic Si slide for detection.Three heavy metals(cadmium(Cd),barium(Ba),and chromium(Cr))were analyzed to evaluate the stability and sensitivity of the ISA method for NELIBS.The results(Cd:RSD=3.6%,LoD=0.654 mg/L;Ba:RSD=3.4%,LoD=0.236 mg/L;Cr:RSD=7.7%,LoD=1.367 mg/L)demonstrated signal enhancement and high-stable and ultrasensitive detection.The actual sample detection(Cd:RE=7.71%,Ba:RE=6.78%)illustrated great reliability.The ISA method,creating a uniform distribution of NP-analytes at the interface,has promising prospects in NELIBS.
文摘Gastric cancer(GC)is a prevalent malignant tumor within the digestive system,with over 40%of new cases and deaths related to GC globally occurring in China.Despite advancements in treatment modalities,such as surgery supplemented by adjuvant radiotherapy or chemotherapeutic agents,the prognosis for GC remains poor.New targeted therapies and immunotherapies are currently under invest-igation,but no significant breakthroughs have been achieved.Studies have indicated that GC is a heterogeneous disease,encompassing multiple subtypes with distinct biological characteristics and roles.Consequently,personalized treatment based on clinical features,pathologic typing,and molecular typing is crucial for the diagnosis and management of precancerous lesions of gastric cancer(PLGC).Current research has categorized GC into four subtypes:Epstein-Barr virus-positive,microsatellite instability,genome stability,and chromosome instability(CIN).Technologies such as multi-omics analysis and gene sequencing are being employed to identify more suitable novel testing methods in these areas.Among these,ultrasensitive chromosomal aneuploidy detection(UCAD)can detect CIN at a genome-wide level in subjects using low-depth whole genome sequencing technology,in conjunction with bioinformatics analysis,to achieve qualitative and quantitative detection of chromosomal stability.This editorial reviews recent research advancements in UCAD technology for the diagnosis and management of PLGC.
基金supported by the National Basic Research Program of China(2015CB932600,2013CB933000)the National Natural Science Foundation of China(21505101,21375042,21405054, 21404097)1000 Young Talent(to Fan Xia) and Zhejiang Provincial Natural Science Foundation of China(LQ16B050003)
文摘The fabrication of sensitive sensors with high selectivity is highly desirable for the detection of some important biomarkers,such as nucleic acids,proteins,small molecules and ions.DNA hybridization chain reaction(HCR) and DNA supersandwich self-assembly(SSA) are two prevalent enzyme-free signal amplification strategies to improve sensitivity of the sensors.In this review,we firstly describe the characteristics about DNA HCR and DNA SSA,and then summarize the advances in the one-dimensional DNA nanostructures assisted by HCR and SSA.This review has been divided into three parts according to the two signal amplification methods and highlights recent progress in these two strategies to improve the detection sensitivity of proteins,nucleic acids,small molecules and ions.
基金the National Natural Science Foundation of China(Nos.22004051 and 22072060)the Natural Science Foundation of Jiangsu Province(No.BK20180858)+4 种基金the China Postdoctoral Science Foundation(No.2019M661733)the Scientific Research Starting Foundation for Senior Talent at Jiangsu University(No.18JDG025)Science Foundation of Wuxi Health Committee(No.BJ202220)Top Talent Support Program for young and middle-aged people of WuxiHealth Committee(No.BJ2023105)Opening of Jiangsu Provincial Key Laboratory of Laboratory Medicine(No.JSKLM-T-2024-1).
文摘The simultaneous detection of carcinoembryonic antigen(CEA)and microRNA-21(miR-21)is of significant clinical importance for cancer diagnosis,prognosis evaluation,and therapy monitoring.In this study,we developed a novel electrochemical biosensor utilizing a peptide-self-assembly-engineered signal amplification(PSA-e-SA)nanoarchitectonic strategy to achieve ultrasensitive and simultaneous quantification of these two critical biomarkers.By designing amphiphilic peptides(C_(16)-MB-AptamerCEA and C_(16)-Fc-ssDNA2)as multifunctional probes,the system exploits their dual roles in target recognition and signal amplification.These peptides self-assemble into nanostructures under mild conditions,facilitating enhanced loading of electroactive molecules such as methylene blue(MB)and ferrocene(Fc),thereby significantly amplifying the electrochemical signal.The biosensor achieved detection limits of 0.788 pM for CEA and 0.0357 nM for miR-21,demonstrating remarkable sensitivity enhancements of 18-fold and 3.8-fold compared to unamplified approaches.As a proof-of-concept study,further experiments underscored the excellent reproducibility and stability of the strategy while also demonstrating its applicability when tested with simulated serum samples.Thus,this work not only presents a valuable assay tool for early cancer diagnosis and biomarker analysis but also indicates that this signal amplification strategy based on peptide self-assembly engineering can be extended to detect other disease-related biomarkers,propelling the development of clinical applications for multifunctional biosensors.
基金supported by National Key R&D Program of China under Grant 2022YFE0103300 and 2020YFA0211400.
文摘Exceptional point(EP)is referred to degeneracies in a non-Hermitian system where two or more eigenvalues and their corresponding eigenvectors coalesce.Recently there have been significantly increased interests in harnessing EPs to enhance responsivities and achieve ultrasensitive detections in optics,electronics and acoustics,although there are few similar studies focused on using surface acoustic wave(SAW)sensing technologies,probably due to its great technical challenges.Herein,we proposed a scheme for accessing EPs in an on-chip architecture consisted of coupledSAW-resonators system,forming a passive parity-time(PT)symmetric system.We demonstrated that by tuning additional losses in one of resonators and regulating the system in the proximity of the EP,the sensor exhibited significantly enhanced responses.As an example,we present an EP-based SAW gas sensor,which showed a muchimproved sensitivity compared to that of a conventional delay-line SAW sensor.The fundamental mechanisms behind this excellent sensing performance have been elucidated.
基金National Key Research and Development Program of China(2023YFF0615604)National Natural Science Foundation of China(62192770,62305252,62205246,62475192,61925504,62020106009,62192771)+3 种基金Science and Technology Commission of Shanghai Municipality(21JC1406100,22ZR1432400)Shanghai Municipal Science and Technology Major Project(2021SHZDZX0100)Shanghai Pilot Program for Basic ResearchFundamental Research Funds for the Central Universities。
文摘Circular dichroism(CD)spectroscopy,widely used for chiral sensing,has been limited by the detection sensitivity.Enhancing optical chirality in the light fields interacting with chiral molecules is crucial for achieving ultrasensitive chiral detection.Here,we present a new paradigm for ultrasensitive chiral detection by creating accessible chiral hotspots using a toroidal dipole Fabry–Perot bound state in the continuum(TD FP-BIC)metasurface.BIC resonance is achieved by controlling the coupling between the TD resonance and its multilayer reflector-induced perfect mirror image.This method enables unprecedented local maximum and average optical chirality enhancements of up to 6×10^(4)-fold and 2×10^(3)-fold,respectively,within non-structured regions,resulting in an 866-fold increase in CD signals compared to chiral molecules alone without nanostructures.Our results pave the way for enhanced light–matter interactions and ultrasensitive enantiomeric operation.
