For today it is known, that primary and secondary disorders of the aerobic respiration, which are based on mitochondrial deficiency, lead to a wide spectrum of clinical manifestations and diseases. Therefore, the ques...For today it is known, that primary and secondary disorders of the aerobic respiration, which are based on mitochondrial deficiency, lead to a wide spectrum of clinical manifestations and diseases. Therefore, the question about effective correction of various types of energy exchange disorders remains topical. Thus, the aim of our work was the study effect of the complex of biologically active substances (BAS) in ultra low concentrations on the activity of key enzymes of aerobic energy metabolism succinate dehydrogenase (EC 1.3.99.1) (SQR) and mitochondrial glycerol-3-phosphate dehydrogenase (EC 1.1.99.5) (GPD2). The human lymphocytes assays were tested in vitro (22 donors). In negative control lymphocytes, cell culture normal saline solution was added. Normal saline solution with NaN3 was added in positive control lymphocytes cell culture. Experimental cell culture contained NaN3 and BAS. Our investigations had been revealed increase SQR activity in the experimental cell culture as compared with positive control culture throughout the time of experiment (measurements were carried out at 4, 24, 48, 72 h of incubation). The SQR activity of experimental cell culture and negative control lymphocytes cell culture was equal up to 24 h of experiment. It showed neutralization of NaN3 inhibitory effect (during 24 h) due to BAS influence. Activity of base glycerophosphate shunt ferment GPD2 of experimental lymphocyte cell culture was not different from GPD2 index in the negative control, but was lower than GPD2 activity in the positive control. It also indicated neutralization NaN3 inhibitory effect due to BAS influence. So we had found that extremely low concentrations of selected BAS with their complex impact on human lymphocytes in vitro could effectively neutralize the inhibitory effect of NaN3 on processes of aerobic energy metabolism link.展开更多
Rapid and accurate detection of ultralow-concentration nanoparticles is crucial forapplications ranging from medical diagnosis to water quality monitoring, yet remainschallenging for current laser-based and light-scat...Rapid and accurate detection of ultralow-concentration nanoparticles is crucial forapplications ranging from medical diagnosis to water quality monitoring, yet remainschallenging for current laser-based and light-scattering methods.While nanoparticletranslocation-based nanopore sensing offers single-particle resolution, conventionalsingle-nanopore resistive pulse sensing approaches suffer from low capture frequency,transient signals, and clogging issues, limiting their effectiveness at extremelylow concentrations. Here, we present a novel nanopore array blockage-based sensingstrategy for the rapid detection and quantification of ultralow-concentrationnanoparticles. Using hydraulic force, nanoparticles are driven through an array ofsubnanoparticle-sized pores, and optical microscopy monitors blockage progression toobtain quantitative concentration data. Our results demonstrate a linear correlationbetween the initial blockage rate and nanoparticle concentration, enabling the detectionof fluorescent nanoparticles down to 0.5 aM (300 particles/mL) within 5 min-athree-order-of-magnitude improvement in sensitivity over previous nanopore-basedmethods. Additionally, our approach can leverage fluorescent nanoparticles as probesto detect unlabeled nanoparticles and contaminants at similarly low concentrations.This strategy provides a robust, efficient, and rapid platform for ultrasensitive nanoparticledetection, with promising applications in biomedical research, environmentalmonitoring, and industrial quality control.展开更多
The development of green and simple chemiluminescence(CL)systems with intensive and long-lasting emission is highly desirable in lighting and extension of their applications.In this study,it is found that the involvem...The development of green and simple chemiluminescence(CL)systems with intensive and long-lasting emission is highly desirable in lighting and extension of their applications.In this study,it is found that the involvement of aggregation-induced emission(AIE)surfactant could greatly enhance the CL of luminol–H2O2–Co2+system.The inserted hydrophobic tetraphenylethylene fluorophore in AIE is able to increase the hydrophobicity of alkyl chain and decrease the critical micelle concentration(CMC)of surfactant.The synergistic effect of micelle-improved enrichment and CL resonance energy transfer endows luminol–H2O2–Co2+system intensive and long-lasting emission under neutral pH conditions(pH 7.4).The visible emission is still observed even after 60 min.Our study has opened a new avenue for exploring green and simple effective CL systems through AIE surfactant with unltralow CMC toward various applications in lighting,optical sensing,and photocatalysis,etc.展开更多
文摘For today it is known, that primary and secondary disorders of the aerobic respiration, which are based on mitochondrial deficiency, lead to a wide spectrum of clinical manifestations and diseases. Therefore, the question about effective correction of various types of energy exchange disorders remains topical. Thus, the aim of our work was the study effect of the complex of biologically active substances (BAS) in ultra low concentrations on the activity of key enzymes of aerobic energy metabolism succinate dehydrogenase (EC 1.3.99.1) (SQR) and mitochondrial glycerol-3-phosphate dehydrogenase (EC 1.1.99.5) (GPD2). The human lymphocytes assays were tested in vitro (22 donors). In negative control lymphocytes, cell culture normal saline solution was added. Normal saline solution with NaN3 was added in positive control lymphocytes cell culture. Experimental cell culture contained NaN3 and BAS. Our investigations had been revealed increase SQR activity in the experimental cell culture as compared with positive control culture throughout the time of experiment (measurements were carried out at 4, 24, 48, 72 h of incubation). The SQR activity of experimental cell culture and negative control lymphocytes cell culture was equal up to 24 h of experiment. It showed neutralization of NaN3 inhibitory effect (during 24 h) due to BAS influence. Activity of base glycerophosphate shunt ferment GPD2 of experimental lymphocyte cell culture was not different from GPD2 index in the negative control, but was lower than GPD2 activity in the positive control. It also indicated neutralization NaN3 inhibitory effect due to BAS influence. So we had found that extremely low concentrations of selected BAS with their complex impact on human lymphocytes in vitro could effectively neutralize the inhibitory effect of NaN3 on processes of aerobic energy metabolism link.
基金USDA-NIFA,Grant/Award Number:2024-67014-42672BU MSE Special User-Charge Grant。
文摘Rapid and accurate detection of ultralow-concentration nanoparticles is crucial forapplications ranging from medical diagnosis to water quality monitoring, yet remainschallenging for current laser-based and light-scattering methods.While nanoparticletranslocation-based nanopore sensing offers single-particle resolution, conventionalsingle-nanopore resistive pulse sensing approaches suffer from low capture frequency,transient signals, and clogging issues, limiting their effectiveness at extremelylow concentrations. Here, we present a novel nanopore array blockage-based sensingstrategy for the rapid detection and quantification of ultralow-concentrationnanoparticles. Using hydraulic force, nanoparticles are driven through an array ofsubnanoparticle-sized pores, and optical microscopy monitors blockage progression toobtain quantitative concentration data. Our results demonstrate a linear correlationbetween the initial blockage rate and nanoparticle concentration, enabling the detectionof fluorescent nanoparticles down to 0.5 aM (300 particles/mL) within 5 min-athree-order-of-magnitude improvement in sensitivity over previous nanopore-basedmethods. Additionally, our approach can leverage fluorescent nanoparticles as probesto detect unlabeled nanoparticles and contaminants at similarly low concentrations.This strategy provides a robust, efficient, and rapid platform for ultrasensitive nanoparticledetection, with promising applications in biomedical research, environmentalmonitoring, and industrial quality control.
基金National Natural Science Foundation of China,Grant/Award Numbers:U22A20397,21974008,22074005Natural Science Foundation of Beijing Municipality,Grant/Award Number:2202038。
文摘The development of green and simple chemiluminescence(CL)systems with intensive and long-lasting emission is highly desirable in lighting and extension of their applications.In this study,it is found that the involvement of aggregation-induced emission(AIE)surfactant could greatly enhance the CL of luminol–H2O2–Co2+system.The inserted hydrophobic tetraphenylethylene fluorophore in AIE is able to increase the hydrophobicity of alkyl chain and decrease the critical micelle concentration(CMC)of surfactant.The synergistic effect of micelle-improved enrichment and CL resonance energy transfer endows luminol–H2O2–Co2+system intensive and long-lasting emission under neutral pH conditions(pH 7.4).The visible emission is still observed even after 60 min.Our study has opened a new avenue for exploring green and simple effective CL systems through AIE surfactant with unltralow CMC toward various applications in lighting,optical sensing,and photocatalysis,etc.
