Brewer's spent grains(BSG),a major by-product of the brewing industry,represent an abundant and sustainable resource for bioactive compounds like ferulic acid(FA),a potent natural antioxidant.This study utilized c...Brewer's spent grains(BSG),a major by-product of the brewing industry,represent an abundant and sustainable resource for bioactive compounds like ferulic acid(FA),a potent natural antioxidant.This study utilized chemical,enzymatic,and hydrothermal hydrolysis methods to extract FA,achieving the highest encapsulation efficiencies(97-98%)through enzymatic and hydrothermal processes.By incorporating FA into advanced nano-delivery systems,the research demonstrated enhanced stability,bioavailability,and regenerative properties in in vitro models,including HaCaT and human dermal fibroblast(HDF)cells,as well as 3D reconstructed epidermis.These systems promoted wound closure(50%in 6 h and 100%in 24 h)and reduced reactive oxygen species levels by 94%,highlighting their efficacy.This work showcases a circular economy approach,transforming BSG from waste into a valuable resource,paving the way for sustainable applications in cosmetics and pharmaceuticals.展开更多
基金supported by the BiOBreW project,which is funded by the European Union’s Horizon MSCA 2021-PF-01 research and innovation programme,under grant agreement N°101065428funded by AGROALNEXT program(AGROALNEXT/2022/060-Desarrollo y optimizacion de procesos innovadores y sostenibles de extraccion de aceite y proteínas a partir de microalgas,insectos,residuos y subproductos agroalimentarios:Evaluacion de propiedades biologicas(EXTRAOLIOPRO))+5 种基金supported by MICIU with funding from European Union NextGenerationEU(PRTR-C17.I1)by Generalitat Valenciana.Finally,M.M-N acknowledges the financial support from ACIF grant funded by Generalitat ValencianaConselleria d’Innovacio,Universitats,Ciencia i Societat Digital(ref:CIACIF/2022/341)support given in Project TED2021-129837B-C42supported by the Regional Government of Castilla y Leon(Spain)and the EU-FEDER program(UIC379)Danilo Cantero is funded by the Spanish Ministry of Science,Innovation and Universities(“Beatriz Galindo”fellowship BEAGAL18/00247).
文摘Brewer's spent grains(BSG),a major by-product of the brewing industry,represent an abundant and sustainable resource for bioactive compounds like ferulic acid(FA),a potent natural antioxidant.This study utilized chemical,enzymatic,and hydrothermal hydrolysis methods to extract FA,achieving the highest encapsulation efficiencies(97-98%)through enzymatic and hydrothermal processes.By incorporating FA into advanced nano-delivery systems,the research demonstrated enhanced stability,bioavailability,and regenerative properties in in vitro models,including HaCaT and human dermal fibroblast(HDF)cells,as well as 3D reconstructed epidermis.These systems promoted wound closure(50%in 6 h and 100%in 24 h)and reduced reactive oxygen species levels by 94%,highlighting their efficacy.This work showcases a circular economy approach,transforming BSG from waste into a valuable resource,paving the way for sustainable applications in cosmetics and pharmaceuticals.
