Objective To observe the differences in therapeutic effects on primary trigeminat neuralgia between contralateral needling technique and routine acupuncture. Methods One hundred and thirty cases were randomly divided ...Objective To observe the differences in therapeutic effects on primary trigeminat neuralgia between contralateral needling technique and routine acupuncture. Methods One hundred and thirty cases were randomly divided into contralateral needling group and routine acupuncture group, 65 cases in each one. In contralateral needling group, contralateral needling technique was adopted on the acupoints on the opposite side corresponding to the affected face in neuralgia. According to the localization of trigeminal neuralgia, the points were selected. For example, for ophthalmologic branch, Yuyao （鱼腰 EX- HN 4） and Touwei （头维 ST 8） were selected as the main point. In routine acupuncture group, the corresponding acupoints were selected on the affected side of the face. The points were same as that in contralateral needling group, Three courses of treatment were required in two groups. The clinical efficacy was evaluated 4 weeks after the end of treatment. Results The total effective rate was 95.4% （62/65） in contralateral needling group, which was superior to that in routine acupuncture group E86.2% （56/65）1 （P〈0. 001）. Conclusion Contralateral needling technique achieves apparently superior effects on primary trigeminal neuralgia compared with routine acupuncture.展开更多
The combination of needling technique of Mind Regulation with Taoism Concentration is to promote the propagated sensation along the channels （PSC） and the needling sensation to the affected area in order to activate...The combination of needling technique of Mind Regulation with Taoism Concentration is to promote the propagated sensation along the channels （PSC） and the needling sensation to the affected area in order to activate the potential regulation of human body. Prof. Yang Yun-kuan used this combination to treat some pain diseases, nervous system＇s diseases and intractable chronic diseases and got a better result than the general techniques, This is the introduction to the manipulation of this technique and its clinical application.展开更多
Objective To discuss the therapeutic effect and mechanism of warming needling technique for the treatment of depression. Methods A total of 60 patients with depression were randomly divided into treatment group and co...Objective To discuss the therapeutic effect and mechanism of warming needling technique for the treatment of depression. Methods A total of 60 patients with depression were randomly divided into treatment group and control group with 30 cases each. The treatment group was treated with warming needle technique on the Jiaji points of the heart, lung, liver, spleen and kidney, control group with Amitriptyline, HAMD scale and the qualitative change of EEG-α wave were observed before and after the treatment. Results The total effective rate of treatment group was 100%, much better than that of the control group with 80% (P<0.05); the HAMD scale of treatment group was better than that of control group (P<0.05); the comparison of the improvement of somatic symptoms between treatment group and control group was significant (P<0.05); the comparison of frequency and amplitude of EEG-α wave was significant (P<0.05), there was no toxic and side effect in the treatment group. Conclusion Warming needling technique for depression has better effect than Amitriptyline, and there was no toxic and side effect in the treatment group. Warming needle technique can effectively regulate the frequency and amplitude EEG-α wave, and acts to improve depressive condition.展开更多
The combined treatment of cangguitanxue(green turtle probing its cave)needling technique and cupping method achieved the significant therapeutic effects on 1 case of traumatic soft tissue injury.Such therapeutic metho...The combined treatment of cangguitanxue(green turtle probing its cave)needling technique and cupping method achieved the significant therapeutic effects on 1 case of traumatic soft tissue injury.Such therapeutic method provides the reference for the clinical treatment of soft tissue swelling due to injury.展开更多
AIM: To compare the success rates and adverse events of early needle-knife fistulotomy(NKF) and double-guidewire technique(DGT) in patients with repetitive unintentional pancreatic cannulations.METHODS: From a total o...AIM: To compare the success rates and adverse events of early needle-knife fistulotomy(NKF) and double-guidewire technique(DGT) in patients with repetitive unintentional pancreatic cannulations.METHODS: From a total of 1650 patients admitted for diagnostic or therapeutic endoscopic retrograde cholangiopancreatography(ERCP) at a single tertiary care hospital(Pusan National University Yangsan Hospital, Yangsan, South Korea) between January2009 and December 2012, 134(8.1%) patients with unsuccessful biliary cannulation after 5 min trial of conventional methods, together with 5 or more repetitive unintentional pancreatic cannulations, were enrolled in the study. Early NKF and DGT groups were assigned 67 patients each. In the DGT group, NKF was performed for an additional 7 min if successful cannulation was not achieved.RESULTS: The success rates with early NKF andthe DGT were 79.1%(53/67) and 44.8%(30/67)(P< 0.001), respectively. The incidence of post-ERCP pancreatitis(PEP) was lower in the early NKF group than in the DGT group [4.5%(3/67) vs 14.9%(10/67),P = 0.041]. The mean cannulation times in the early NKF and DGT groups after assignment were 257 s and312 s(P = 0.013), respectively.CONCLUSION: Our data suggest that early NKF should be considered as the first approach to selective biliary cannulation in patients with repetitive unintentional pancreatic cannulations.展开更多
Since its initial report in 1992,endoscopic ultrasoundguided fine needle aspiration(EUS-FNA) has now been incorporated into the diagnostic and staging algorithm for the evaluation of benign and malignant diseases of t...Since its initial report in 1992,endoscopic ultrasoundguided fine needle aspiration(EUS-FNA) has now been incorporated into the diagnostic and staging algorithm for the evaluation of benign and malignant diseases of the gastrointestinal tract and of adjacent organs.Its introduction constitutes a major breakthrough in the endoscopic field and has gradually transformed EUS from a pure imaging modality into a more interventional procedure.In addition,the possibility of collecting samples,providing a definitive cytological and/or histological evidence of the presence of malignancy,has strongly contributed to changing EUS from a subjective,highly operator dependant procedure into a more objective one.This article will review the instrumentation,technique and the most important clinical applications of EUS-FNA.展开更多
BACKGROUND Other than surgery,endoscopic ultrasound-guided fine-needle aspiration(EUSFNA)is the only procedure for histologically diagnosing autoimmune pancreatitis(AIP).However,adequate specimens are difficult to obt...BACKGROUND Other than surgery,endoscopic ultrasound-guided fine-needle aspiration(EUSFNA)is the only procedure for histologically diagnosing autoimmune pancreatitis(AIP).However,adequate specimens are difficult to obtain.Recently,more adequate specimens were reported to be obtained with EUS-FNA with a wet suction technique(WEST)than with conventional EUS-FNA.AIM To histologically diagnose AIP by EUS-FNA with a WEST.METHODS Eleven patients with possible type 1 AIP between February 2016 and August 2018 underwent EUS-FNA with a WEST(WEST group),with four punctures by 19 or 22 G needles.As a historical control,23 type 1 AIP patients who underwent no fewer than four punctures with 19 or 22 G needles were enrolled(DRY group).Patient characteristics and histological findings were compared between the two groups.RESULTS Three histopathological factors according to the International Consensus Diagnostic Criteria were significantly greater in the WEST group than the DRY group[lymphoplasmacytic infiltrate without granulocytic infiltration:9(81.8%)vs 6(26.1%),P=0.003,storiform fibrosis:5(45.5%)vs 1(4.3%),P=0.008,abundant(>10 cells/HPF)IgG4-positive cells:7(63.6%)vs 5(21.7%),P=0.026].Level 1 or level 2 histopathological findings were observed more often in the WEST group than in the DRY group[8(72.7%)vs 3(13.0%),P=0.001].CONCLUSION EUS-FNA with a WEST was more successful than standard EUS-FNA in histologically diagnosing AIP.展开更多
Background:Nummular eczema(NE)is an inflammatory skin disease characterized by pruritic coinshaped lesions and a long-lasting and easy-relapsing course,which severely decreases patients’quality of life.This case was ...Background:Nummular eczema(NE)is an inflammatory skin disease characterized by pruritic coinshaped lesions and a long-lasting and easy-relapsing course,which severely decreases patients’quality of life.This case was designed to observe the effects of specific acupuncture techniques on pruritic NE management.Case presentation:A 22-year-old female was first diagnosed with pruritic NE three years earlier.No allergies were found in the patch test;while emotional stress would trigger and aggravate the NE.External application of 0.1%tacrolimus failed to reverse this condition over a long period of treatment.Treatment&outcomes:An individualized acupuncture treatment protocol was identified.Half needling and encircling needling were applied at local eczematous lesions,with routine needling at meridian points,once per week,for 12 treatment sessions.All outcome measures,including the Eczema Area and Severity Index,Visual Analogue Scale score for pruritus,Self-Rating Anxiety Score,and Dermatology Quality Life Index,substantially improved over 6 months from pre-treatment to 3 months posttreatment.Conclusion:Acupuncture might be a promising non-pharmacological treatment method for patients with NE,especially for those with emotional stress.A randomized controlled trial with an adequate sample size and rigorous study design is required to verify the results of the study.展开更多
Objective:To observe the clinical effect on superior cluneal nerve(SCN)entrapment syndrome treated with the release technique of long round-sharp needle.Methods:The syndrome differentiation based on meridian muscle re...Objective:To observe the clinical effect on superior cluneal nerve(SCN)entrapment syndrome treated with the release technique of long round-sharp needle.Methods:The syndrome differentiation based on meridian muscle region was adopted.The release technique of the long round-sharp needle was used at the lesions of meridian tendon region,Yāoyícì(Beside Yaoyi)and the transverse process of the third lumbar vertebra in 34 patients with SCN entrapment syndrome.The treatment was given once a week,4 treatments made one course.After one course treatment,the therapeutic effect was observed.The results of pain rating index(PRI),the visual analogy scores(VAS)and the present pain intensity(PPI)were compared before and after treatment.Results:Of 34 patients,28 cases(82%)were cured,6 cases(18%)effective and 0 case(0%)failed.The total effective rate was 100%.The scores of PRI,VAS and PPI were(10.78±1.98),(5.98±1.19)and(3.91±1.68)successively in 34 cases before treatment and they were(1.98±1.79),(0.89±1.12)and(0.82±0.79)after treatment.The score of every evaluation scale after treatment was lower significantly than that before treatment(all P<0.01).Conclusion:The release technique of long round-sharp needle achieves the satisfactory clinical effect on SCN entrapment syndrome.Hence,this therapeutic method deserves to be promoted.展开更多
BACKGROUND ： Experimental animal models of intracerebral hemorrhag （ICH） are greatly needed, so the process of establishment should be ideal in hematoma formation and easy to operate. OBJECTIVE ： To construct mode...BACKGROUND ： Experimental animal models of intracerebral hemorrhag （ICH） are greatly needed, so the process of establishment should be ideal in hematoma formation and easy to operate. OBJECTIVE ： To construct model of ICH in rats with double injection of autologous blood taken from the cut tail cut and double withdrawal of the needle （shortened as two-step injection model）, and compare with those induced by single and double injections. DESIGN ： A randomized controlled tria SETTING： Department of Neurology, General Hospital of Military Area Command of Chinese PLA. MATERIALS： Thirty male Wistar rats of 10 to 12 months, weighing （400±25） g, provided by the Experimental Center of Medical Animals, General Hospital of Shenyang Military Area Command of Chinese PLA, were divided randomly into 3 groups with 10 rats in each group： two-step injection group, single injection group, double injection group. METHODS ： The experiment was carried out in the Department of Neurology, General Hospital of Military Area Command of Chinese PLA from March to June in 2004. Autologous blood ICH model in rats were established as follows： In the two-step injection group, 50 μL unclotted autologous blood was taken from the rat tail cut, then injected with microsyringe into the caudate nucleus, 10 μL injected at first, paused for 2 minutes, and then the rest 40 μL injected slowly and continuously within 2 minutes. After the injection, the needle was kept immovable for about 4 minutes, withdrawn 2.0 mm, again kept immovable for about 4 minute, and then removed wholly at a slow speed. In the single injection group, 50 μL unclotted tail blood was injected slowly and continuously all within 2 minutes and the needle was slowly removed;（4） In the double injection group, 10 mL blood was injected at first, paused for 2 minutes, the rest 40 μL injected evenly within 2 minutes, and then the needle was withdrawn slowly and uninterruptedly. Neurologic findings were scored in accordance with Longa＇s five-point scale （0-4 scores, the higher the score, the severer the neurological dysfunction）. The rats were killed to remove and sections were prepared, the morphological features of hematomas were grossly observed, the maximal diameter and size of hematomas in each slice were measured with the imaging analytical system, and the volume was calculated. Meanwhile, the conveniences of the techniques were compared. MAIN OUTCOME MEASURES： The morphological features and volume of hematomas, neurologic deficit score （NDS）, and the convenience of the techniques were compared. RESULTS： All the 30 rats were involved in the analysis of results without deletion. （1） Results of the morphological observation of volume of hematoma： In the two-step injection group, hematomas located in the right caudate nucleus area regularly in circular or analogously circular shape in each slice. The formation rate of hematomas in the single injection group and double injection group were lower than those in the two-step injection group [60% （6/10）, 80% （8/10）, 100% （10/10）, P〈 0.01, 0.05]. The volume of hematomas in the single injection group and double injection group were smaller than those in the two-step injection group [（28.5±14.8）, （33.4±7.4）, （41.6±3.9） mm3, P〈 0.01, 0.05]. （2） NDS results： The NDS scores in the single injection group and double injection group were smaller than that in the two-step injection group （0.90±0.83, 1.30±0.78, 1.90±0.57, P〈 0.05）. （3）Comparison of the convenience of the techniques： The double injection method allowed generating reproducible hematomas in rats with shortcomings that it needed autologous arterial blood from femoral artery, and precision instruments such as microinfusion pump. The two-step injection injected fresh unclotting blood taken directly from the tail cut with microsyringe into the rat brain, and it has the advantages of easy operation, no influence on the activity of thrombase, shorter duration for model establishment, and higher rate of hematoma formation, which could generate ideal and economical models of ICH. The two-step injection induced hemotoma regularly in circular or analogously circular shape in each slice, but those induced by single and double injections were mostly in strip or fusiform shapes and extending along the needle tracks or into the ventricle or subarachnoid space. CONCLUSION ： The autologous blood ICH model induced by the two-step injection method is a reproducible and reliable one in regular shape, which is better than those induced by double and single injections.展开更多
Objective: to observe the application effect of detail optimization puncture technique when using disposable scalp needle in childrens scalp intravenous infusion. Methods: 200 children with scalp venous infusion were ...Objective: to observe the application effect of detail optimization puncture technique when using disposable scalp needle in childrens scalp intravenous infusion. Methods: 200 children with scalp venous infusion were received from January 2021 to January 2022, and standardized grouping operation was carried out randomly. The control group applied routine puncture technique to 100 cases included, and the observation group applied detail optimized puncture technique to 100 cases included. The pain score, puncture condition, adverse condition and family satisfaction were compared between groups. Results: the scores of the former group were lower than that of the latter group (P < 0.05). The success rate of one-time puncture was 94.00% in the observation group and 76.00% in the control group. The former group was higher than the latter group, and the puncture time in the former group was lower (P < 0.05);the adverse situation of the children included in the observation group was evaluated as 2.00%, and that of the control group was evaluated as 14.00%. The former group was lower than the latter group (P < 0.05);the satisfaction of the family members of the children included in the observation group was 100.00%, and the satisfaction of the family members of the control group was 90.00%. The former group was higher than the latter group (P < 0.05). Conclusion: the implementation of detail optimization puncture technique in scalp vein infusion with one-time scalp needle puncture can improve the success rate of one-time puncture, reduce the pain of children and make the family satisfied.展开更多
文摘Objective To observe the differences in therapeutic effects on primary trigeminat neuralgia between contralateral needling technique and routine acupuncture. Methods One hundred and thirty cases were randomly divided into contralateral needling group and routine acupuncture group, 65 cases in each one. In contralateral needling group, contralateral needling technique was adopted on the acupoints on the opposite side corresponding to the affected face in neuralgia. According to the localization of trigeminal neuralgia, the points were selected. For example, for ophthalmologic branch, Yuyao （鱼腰 EX- HN 4） and Touwei （头维 ST 8） were selected as the main point. In routine acupuncture group, the corresponding acupoints were selected on the affected side of the face. The points were same as that in contralateral needling group, Three courses of treatment were required in two groups. The clinical efficacy was evaluated 4 weeks after the end of treatment. Results The total effective rate was 95.4% （62/65） in contralateral needling group, which was superior to that in routine acupuncture group E86.2% （56/65）1 （P〈0. 001）. Conclusion Contralateral needling technique achieves apparently superior effects on primary trigeminal neuralgia compared with routine acupuncture.
文摘The combination of needling technique of Mind Regulation with Taoism Concentration is to promote the propagated sensation along the channels （PSC） and the needling sensation to the affected area in order to activate the potential regulation of human body. Prof. Yang Yun-kuan used this combination to treat some pain diseases, nervous system＇s diseases and intractable chronic diseases and got a better result than the general techniques, This is the introduction to the manipulation of this technique and its clinical application.
文摘Objective To discuss the therapeutic effect and mechanism of warming needling technique for the treatment of depression. Methods A total of 60 patients with depression were randomly divided into treatment group and control group with 30 cases each. The treatment group was treated with warming needle technique on the Jiaji points of the heart, lung, liver, spleen and kidney, control group with Amitriptyline, HAMD scale and the qualitative change of EEG-α wave were observed before and after the treatment. Results The total effective rate of treatment group was 100%, much better than that of the control group with 80% (P<0.05); the HAMD scale of treatment group was better than that of control group (P<0.05); the comparison of the improvement of somatic symptoms between treatment group and control group was significant (P<0.05); the comparison of frequency and amplitude of EEG-α wave was significant (P<0.05), there was no toxic and side effect in the treatment group. Conclusion Warming needling technique for depression has better effect than Amitriptyline, and there was no toxic and side effect in the treatment group. Warming needle technique can effectively regulate the frequency and amplitude EEG-α wave, and acts to improve depressive condition.
基金Supported by Key Research Project Funding Program:2017GSF19116
文摘The combined treatment of cangguitanxue(green turtle probing its cave)needling technique and cupping method achieved the significant therapeutic effects on 1 case of traumatic soft tissue injury.Such therapeutic method provides the reference for the clinical treatment of soft tissue swelling due to injury.
基金Supported by a 2-year Research Grant of Pusan National University
文摘AIM: To compare the success rates and adverse events of early needle-knife fistulotomy(NKF) and double-guidewire technique(DGT) in patients with repetitive unintentional pancreatic cannulations.METHODS: From a total of 1650 patients admitted for diagnostic or therapeutic endoscopic retrograde cholangiopancreatography(ERCP) at a single tertiary care hospital(Pusan National University Yangsan Hospital, Yangsan, South Korea) between January2009 and December 2012, 134(8.1%) patients with unsuccessful biliary cannulation after 5 min trial of conventional methods, together with 5 or more repetitive unintentional pancreatic cannulations, were enrolled in the study. Early NKF and DGT groups were assigned 67 patients each. In the DGT group, NKF was performed for an additional 7 min if successful cannulation was not achieved.RESULTS: The success rates with early NKF andthe DGT were 79.1%(53/67) and 44.8%(30/67)(P< 0.001), respectively. The incidence of post-ERCP pancreatitis(PEP) was lower in the early NKF group than in the DGT group [4.5%(3/67) vs 14.9%(10/67),P = 0.041]. The mean cannulation times in the early NKF and DGT groups after assignment were 257 s and312 s(P = 0.013), respectively.CONCLUSION: Our data suggest that early NKF should be considered as the first approach to selective biliary cannulation in patients with repetitive unintentional pancreatic cannulations.
文摘Since its initial report in 1992,endoscopic ultrasoundguided fine needle aspiration(EUS-FNA) has now been incorporated into the diagnostic and staging algorithm for the evaluation of benign and malignant diseases of the gastrointestinal tract and of adjacent organs.Its introduction constitutes a major breakthrough in the endoscopic field and has gradually transformed EUS from a pure imaging modality into a more interventional procedure.In addition,the possibility of collecting samples,providing a definitive cytological and/or histological evidence of the presence of malignancy,has strongly contributed to changing EUS from a subjective,highly operator dependant procedure into a more objective one.This article will review the instrumentation,technique and the most important clinical applications of EUS-FNA.
文摘BACKGROUND Other than surgery,endoscopic ultrasound-guided fine-needle aspiration(EUSFNA)is the only procedure for histologically diagnosing autoimmune pancreatitis(AIP).However,adequate specimens are difficult to obtain.Recently,more adequate specimens were reported to be obtained with EUS-FNA with a wet suction technique(WEST)than with conventional EUS-FNA.AIM To histologically diagnose AIP by EUS-FNA with a WEST.METHODS Eleven patients with possible type 1 AIP between February 2016 and August 2018 underwent EUS-FNA with a WEST(WEST group),with four punctures by 19 or 22 G needles.As a historical control,23 type 1 AIP patients who underwent no fewer than four punctures with 19 or 22 G needles were enrolled(DRY group).Patient characteristics and histological findings were compared between the two groups.RESULTS Three histopathological factors according to the International Consensus Diagnostic Criteria were significantly greater in the WEST group than the DRY group[lymphoplasmacytic infiltrate without granulocytic infiltration:9(81.8%)vs 6(26.1%),P=0.003,storiform fibrosis:5(45.5%)vs 1(4.3%),P=0.008,abundant(>10 cells/HPF)IgG4-positive cells:7(63.6%)vs 5(21.7%),P=0.026].Level 1 or level 2 histopathological findings were observed more often in the WEST group than in the DRY group[8(72.7%)vs 3(13.0%),P=0.001].CONCLUSION EUS-FNA with a WEST was more successful than standard EUS-FNA in histologically diagnosing AIP.
文摘Background:Nummular eczema(NE)is an inflammatory skin disease characterized by pruritic coinshaped lesions and a long-lasting and easy-relapsing course,which severely decreases patients’quality of life.This case was designed to observe the effects of specific acupuncture techniques on pruritic NE management.Case presentation:A 22-year-old female was first diagnosed with pruritic NE three years earlier.No allergies were found in the patch test;while emotional stress would trigger and aggravate the NE.External application of 0.1%tacrolimus failed to reverse this condition over a long period of treatment.Treatment&outcomes:An individualized acupuncture treatment protocol was identified.Half needling and encircling needling were applied at local eczematous lesions,with routine needling at meridian points,once per week,for 12 treatment sessions.All outcome measures,including the Eczema Area and Severity Index,Visual Analogue Scale score for pruritus,Self-Rating Anxiety Score,and Dermatology Quality Life Index,substantially improved over 6 months from pre-treatment to 3 months posttreatment.Conclusion:Acupuncture might be a promising non-pharmacological treatment method for patients with NE,especially for those with emotional stress.A randomized controlled trial with an adequate sample size and rigorous study design is required to verify the results of the study.
文摘Objective:To observe the clinical effect on superior cluneal nerve(SCN)entrapment syndrome treated with the release technique of long round-sharp needle.Methods:The syndrome differentiation based on meridian muscle region was adopted.The release technique of the long round-sharp needle was used at the lesions of meridian tendon region,Yāoyícì(Beside Yaoyi)and the transverse process of the third lumbar vertebra in 34 patients with SCN entrapment syndrome.The treatment was given once a week,4 treatments made one course.After one course treatment,the therapeutic effect was observed.The results of pain rating index(PRI),the visual analogy scores(VAS)and the present pain intensity(PPI)were compared before and after treatment.Results:Of 34 patients,28 cases(82%)were cured,6 cases(18%)effective and 0 case(0%)failed.The total effective rate was 100%.The scores of PRI,VAS and PPI were(10.78±1.98),(5.98±1.19)and(3.91±1.68)successively in 34 cases before treatment and they were(1.98±1.79),(0.89±1.12)and(0.82±0.79)after treatment.The score of every evaluation scale after treatment was lower significantly than that before treatment(all P<0.01).Conclusion:The release technique of long round-sharp needle achieves the satisfactory clinical effect on SCN entrapment syndrome.Hence,this therapeutic method deserves to be promoted.
文摘BACKGROUND ： Experimental animal models of intracerebral hemorrhag （ICH） are greatly needed, so the process of establishment should be ideal in hematoma formation and easy to operate. OBJECTIVE ： To construct model of ICH in rats with double injection of autologous blood taken from the cut tail cut and double withdrawal of the needle （shortened as two-step injection model）, and compare with those induced by single and double injections. DESIGN ： A randomized controlled tria SETTING： Department of Neurology, General Hospital of Military Area Command of Chinese PLA. MATERIALS： Thirty male Wistar rats of 10 to 12 months, weighing （400±25） g, provided by the Experimental Center of Medical Animals, General Hospital of Shenyang Military Area Command of Chinese PLA, were divided randomly into 3 groups with 10 rats in each group： two-step injection group, single injection group, double injection group. METHODS ： The experiment was carried out in the Department of Neurology, General Hospital of Military Area Command of Chinese PLA from March to June in 2004. Autologous blood ICH model in rats were established as follows： In the two-step injection group, 50 μL unclotted autologous blood was taken from the rat tail cut, then injected with microsyringe into the caudate nucleus, 10 μL injected at first, paused for 2 minutes, and then the rest 40 μL injected slowly and continuously within 2 minutes. After the injection, the needle was kept immovable for about 4 minutes, withdrawn 2.0 mm, again kept immovable for about 4 minute, and then removed wholly at a slow speed. In the single injection group, 50 μL unclotted tail blood was injected slowly and continuously all within 2 minutes and the needle was slowly removed;（4） In the double injection group, 10 mL blood was injected at first, paused for 2 minutes, the rest 40 μL injected evenly within 2 minutes, and then the needle was withdrawn slowly and uninterruptedly. Neurologic findings were scored in accordance with Longa＇s five-point scale （0-4 scores, the higher the score, the severer the neurological dysfunction）. The rats were killed to remove and sections were prepared, the morphological features of hematomas were grossly observed, the maximal diameter and size of hematomas in each slice were measured with the imaging analytical system, and the volume was calculated. Meanwhile, the conveniences of the techniques were compared. MAIN OUTCOME MEASURES： The morphological features and volume of hematomas, neurologic deficit score （NDS）, and the convenience of the techniques were compared. RESULTS： All the 30 rats were involved in the analysis of results without deletion. （1） Results of the morphological observation of volume of hematoma： In the two-step injection group, hematomas located in the right caudate nucleus area regularly in circular or analogously circular shape in each slice. The formation rate of hematomas in the single injection group and double injection group were lower than those in the two-step injection group [60% （6/10）, 80% （8/10）, 100% （10/10）, P〈 0.01, 0.05]. The volume of hematomas in the single injection group and double injection group were smaller than those in the two-step injection group [（28.5±14.8）, （33.4±7.4）, （41.6±3.9） mm3, P〈 0.01, 0.05]. （2） NDS results： The NDS scores in the single injection group and double injection group were smaller than that in the two-step injection group （0.90±0.83, 1.30±0.78, 1.90±0.57, P〈 0.05）. （3）Comparison of the convenience of the techniques： The double injection method allowed generating reproducible hematomas in rats with shortcomings that it needed autologous arterial blood from femoral artery, and precision instruments such as microinfusion pump. The two-step injection injected fresh unclotting blood taken directly from the tail cut with microsyringe into the rat brain, and it has the advantages of easy operation, no influence on the activity of thrombase, shorter duration for model establishment, and higher rate of hematoma formation, which could generate ideal and economical models of ICH. The two-step injection induced hemotoma regularly in circular or analogously circular shape in each slice, but those induced by single and double injections were mostly in strip or fusiform shapes and extending along the needle tracks or into the ventricle or subarachnoid space. CONCLUSION ： The autologous blood ICH model induced by the two-step injection method is a reproducible and reliable one in regular shape, which is better than those induced by double and single injections.
文摘Objective: to observe the application effect of detail optimization puncture technique when using disposable scalp needle in childrens scalp intravenous infusion. Methods: 200 children with scalp venous infusion were received from January 2021 to January 2022, and standardized grouping operation was carried out randomly. The control group applied routine puncture technique to 100 cases included, and the observation group applied detail optimized puncture technique to 100 cases included. The pain score, puncture condition, adverse condition and family satisfaction were compared between groups. Results: the scores of the former group were lower than that of the latter group (P < 0.05). The success rate of one-time puncture was 94.00% in the observation group and 76.00% in the control group. The former group was higher than the latter group, and the puncture time in the former group was lower (P < 0.05);the adverse situation of the children included in the observation group was evaluated as 2.00%, and that of the control group was evaluated as 14.00%. The former group was lower than the latter group (P < 0.05);the satisfaction of the family members of the children included in the observation group was 100.00%, and the satisfaction of the family members of the control group was 90.00%. The former group was higher than the latter group (P < 0.05). Conclusion: the implementation of detail optimization puncture technique in scalp vein infusion with one-time scalp needle puncture can improve the success rate of one-time puncture, reduce the pain of children and make the family satisfied.
文摘目的:观察针刀疏筋解结术治疗椎动脉型颈椎病(CSA)的临床疗效。方法:将76例CSA患者随机分为针刀组(38例,脱落2例)和药物组(38例,脱落2例,剔除1例)。药物组予口服盐酸氟桂利嗪胶囊,每次10 mg,每天1次,连续12 d;针刀组于枕下三角、肩胛提肌止点等太阳经筋在颈肩部所结处予针刀疏筋解结术治疗,每4 d 1次,共治疗3次。分别于治疗前后及治疗结束后3个月随访观察两组患者颈性眩晕症状与功能评估量表(ESCV)和眩晕障碍量表(DHI)评分,于治疗前后采用经颅多普勒超声(TCD)观察患者左侧椎动脉(LVA)、右侧椎动脉(RVA)、基底动脉(BA)的平均血流速度(Vm)及BA血管搏动指数(PI)、阻力指数(RI),并评定两组临床疗效和安全性。结果:治疗后及随访时,两组患者ESCV评分较治疗前升高、DHI评分较治疗前降低(P<0.05);随访时,药物组患者ESCV评分较治疗后降低、DHI评分较治疗后升高(P<0.05)。治疗后及随访时,针刀组患者ESCV评分高于药物组(P<0.05),DHI评分低于药物组(P<0.05)。治疗后,两组患者LVA-Vm、RVA-Vm、BA-Vm较治疗前升高(P<0.05),BA-PI、BA-RI较治疗前降低(P<0.05);针刀组患者LVA-Vm、BA-Vm高于药物组(P<0.05,P<0.01),BA-PI、BA-RI低于药物组(P<0.05,P<0.01)。针刀组总有效率为94.4%(34/36),高于药物组的77.1%(27/35,P<0.01)。两组均未出现不良反应。结论:针刀疏筋解结术可改善CSA患者的眩晕症状和平衡功能,提高椎-基底动脉血管弹性及平均血流速度。
