The published article titled“UCA1 Regulates the Growth and Metastasis of Pancreatic Cancer by Sponging miR-135a”has been retracted from Oncology Research,Vol.25,No.9,2017,pp.1529–1541.DOI:10.3727/096504017X14888987...The published article titled“UCA1 Regulates the Growth and Metastasis of Pancreatic Cancer by Sponging miR-135a”has been retracted from Oncology Research,Vol.25,No.9,2017,pp.1529–1541.DOI:10.3727/096504017X14888987683152 URL:https://www.techscience.com/or/v25n9/56938 Following the publication,concerns have been raised about a number of figures in this article.An unexpected area of similarity was identified in terms of the cellular data,where the results from differently performed experiments were intended to have been shown,although the areas immediately surrounding this area featured comparatively different distributions of cells.展开更多
目的分析急性心肌梗死(AMI)患者发病后血浆尿路上皮癌相关1(UCA1)、肌酸激酶同工酶MB(CK-MB)、肌钙蛋白I(cTnI)变化及其诊断价值。方法选取2016年7月至2017年6月本院收治的80例AMI患者及30例健康志愿者为研究对象,分别纳入AMI组、对照组...目的分析急性心肌梗死(AMI)患者发病后血浆尿路上皮癌相关1(UCA1)、肌酸激酶同工酶MB(CK-MB)、肌钙蛋白I(cTnI)变化及其诊断价值。方法选取2016年7月至2017年6月本院收治的80例AMI患者及30例健康志愿者为研究对象,分别纳入AMI组、对照组,采用实时荧光定量聚合酶链反应法(qPCR)检测UCA1,比较两组入院后0~2、2~6、6~12、12~24、24~48、48~72、72~96 h血浆UCA1及CK-MB、cTnI水平,分析AMI组中合并不同基础疾病(高血压、糖尿病、高脂血症)患者UCA1、CK-MB、cTnI水平差异。结果与对照组比较,AMI组UCA1整体表达水平下调,且AMI组入院后0~48 h UCA1低于对照组(P<0.05),AMI组中UCA1在6~12 h降至最低(P<0.05),后升高(P<0.05),至入院后48~72 h、72~96 h与对照组比较差异无统计学意义(P>0.05);AMI组入院后2~48 h CK-MB、cTnI水平高于对照组(P<0.05),CK-MB在6~12 h达峰值,cTnI在12~24 h达峰值(P<0.05);AMI组中单纯AMI、合并高血压、合并糖尿病、合并高脂血症、合并2种及以上患者UCA1、CK-MB、cTnI表达水平分别两两比较差异无统计学意义(P>0.05);入院后6~12 h UCA1诊断AMI的灵敏度、特异度、准确度、ROC曲线下面积分别为85.42%、83.14%、86.11%、0.954,均较CK-MB、cTnI高。结论AMI患者发病后血浆UCA1表达水平可作为其潜在诊断标志物及治疗靶点,尤其在入院后6~12 h的UCA1有较高监测价值。展开更多
目的克隆新的UCA1剪接变异体全长cDNA序列,为研究其可变剪接机制奠定基础。方法用电子克隆技术和cDNA序列末端快速扩增技术(rapid amplification of cDNA ends,RACE)扩增细胞系BLZ-211cDNA并进行产物测序和序列拼接。结果新克隆的UCA1...目的克隆新的UCA1剪接变异体全长cDNA序列,为研究其可变剪接机制奠定基础。方法用电子克隆技术和cDNA序列末端快速扩增技术(rapid amplification of cDNA ends,RACE)扩增细胞系BLZ-211cDNA并进行产物测序和序列拼接。结果新克隆的UCA1剪接变异体全长cDNA序列为2 202bp。结论综合采用电子克隆技术与RACE技术是获得全长cDNA序列的有效方法,为该基因的后续可变剪接机制的研究奠定了基础。展开更多
文摘The published article titled“UCA1 Regulates the Growth and Metastasis of Pancreatic Cancer by Sponging miR-135a”has been retracted from Oncology Research,Vol.25,No.9,2017,pp.1529–1541.DOI:10.3727/096504017X14888987683152 URL:https://www.techscience.com/or/v25n9/56938 Following the publication,concerns have been raised about a number of figures in this article.An unexpected area of similarity was identified in terms of the cellular data,where the results from differently performed experiments were intended to have been shown,although the areas immediately surrounding this area featured comparatively different distributions of cells.
文摘目的分析急性心肌梗死(AMI)患者发病后血浆尿路上皮癌相关1(UCA1)、肌酸激酶同工酶MB(CK-MB)、肌钙蛋白I(cTnI)变化及其诊断价值。方法选取2016年7月至2017年6月本院收治的80例AMI患者及30例健康志愿者为研究对象,分别纳入AMI组、对照组,采用实时荧光定量聚合酶链反应法(qPCR)检测UCA1,比较两组入院后0~2、2~6、6~12、12~24、24~48、48~72、72~96 h血浆UCA1及CK-MB、cTnI水平,分析AMI组中合并不同基础疾病(高血压、糖尿病、高脂血症)患者UCA1、CK-MB、cTnI水平差异。结果与对照组比较,AMI组UCA1整体表达水平下调,且AMI组入院后0~48 h UCA1低于对照组(P<0.05),AMI组中UCA1在6~12 h降至最低(P<0.05),后升高(P<0.05),至入院后48~72 h、72~96 h与对照组比较差异无统计学意义(P>0.05);AMI组入院后2~48 h CK-MB、cTnI水平高于对照组(P<0.05),CK-MB在6~12 h达峰值,cTnI在12~24 h达峰值(P<0.05);AMI组中单纯AMI、合并高血压、合并糖尿病、合并高脂血症、合并2种及以上患者UCA1、CK-MB、cTnI表达水平分别两两比较差异无统计学意义(P>0.05);入院后6~12 h UCA1诊断AMI的灵敏度、特异度、准确度、ROC曲线下面积分别为85.42%、83.14%、86.11%、0.954,均较CK-MB、cTnI高。结论AMI患者发病后血浆UCA1表达水平可作为其潜在诊断标志物及治疗靶点,尤其在入院后6~12 h的UCA1有较高监测价值。
