A laboratory experiment was conducted to demonstrate the importance of improving the efficiency of six isolates of Aspergillus niger fungi.Four isolates were exposed to UV-rays radiation at a distance of 30 cm,a wavel...A laboratory experiment was conducted to demonstrate the importance of improving the efficiency of six isolates of Aspergillus niger fungi.Four isolates were exposed to UV-rays radiation at a distance of 30 cm,a wavelength of 254–255 nm during M15,M30,and M45 minutes.Exposure periods,both of wild(no UV-rays exposure)and no A.niger(C)as controls,all six isolates were identified molecularly by polymerase chain reaction technique extracted DNA of A.niger was analyzed to ensure gene completion through multiple sequence alignment by bioinformatic programs to study the improvement of dissolving efficiency of mutant and wild A.niger.They were incubated after the addition of superphosphate fertilizer(47%P2O5)at 90 mg P kg–1 applied to soil with controls(no A.niger or no superphosphate)for I,II,III,and IV weeks of incubation periods at 28±1°C.In addition,the DNA extraction and purification by NanoDrop of Thermo Scientific-200 A280/A260 ratio was 2.01 and confirmed that the sequences of nitrogenous bases by the method of multiple sequence alignment(MSA)as compared to the reference sequence of A.niger recorded in the gene bank under the accession number LC632396.Results proved that the M30 minutes exposure UV-rays radiation period was the superior dose when mutant Aspergillus niger obtained the highest amount of dissolved phosphate,reducing soil-pH with maximum biomass of A.niger during the M30 isolate during the third week(III).展开更多
The study illustrates differences between colors of poplar I-214 veneers dyed by reactive blue KN-R and 4 kinds of chemical reagents, and those of treated veneer exposed to UV-ray for 3 days. The results show there ar...The study illustrates differences between colors of poplar I-214 veneers dyed by reactive blue KN-R and 4 kinds of chemical reagents, and those of treated veneer exposed to UV-ray for 3 days. The results show there are some laws in poplar I-214 dyeing and UV-ray attenuation of each chemically treated veneer color. The chemical reaction carded out between cellulose or lignin on veneer surface and dye molecules when they met with. The capability of dyeing and discoloration of veneer is improved by control of dyeing technology, especially chemical treatments; being treated by acetylation and maleic anhydride protects dyed wood discoloration.展开更多
文摘A laboratory experiment was conducted to demonstrate the importance of improving the efficiency of six isolates of Aspergillus niger fungi.Four isolates were exposed to UV-rays radiation at a distance of 30 cm,a wavelength of 254–255 nm during M15,M30,and M45 minutes.Exposure periods,both of wild(no UV-rays exposure)and no A.niger(C)as controls,all six isolates were identified molecularly by polymerase chain reaction technique extracted DNA of A.niger was analyzed to ensure gene completion through multiple sequence alignment by bioinformatic programs to study the improvement of dissolving efficiency of mutant and wild A.niger.They were incubated after the addition of superphosphate fertilizer(47%P2O5)at 90 mg P kg–1 applied to soil with controls(no A.niger or no superphosphate)for I,II,III,and IV weeks of incubation periods at 28±1°C.In addition,the DNA extraction and purification by NanoDrop of Thermo Scientific-200 A280/A260 ratio was 2.01 and confirmed that the sequences of nitrogenous bases by the method of multiple sequence alignment(MSA)as compared to the reference sequence of A.niger recorded in the gene bank under the accession number LC632396.Results proved that the M30 minutes exposure UV-rays radiation period was the superior dose when mutant Aspergillus niger obtained the highest amount of dissolved phosphate,reducing soil-pH with maximum biomass of A.niger during the M30 isolate during the third week(III).
基金This research was conducted in Japan under the Research Planning of Japan-China Cooperation Project (the Research Project onTimber from Plantion Forests in China: JICA Project).
文摘The study illustrates differences between colors of poplar I-214 veneers dyed by reactive blue KN-R and 4 kinds of chemical reagents, and those of treated veneer exposed to UV-ray for 3 days. The results show there are some laws in poplar I-214 dyeing and UV-ray attenuation of each chemically treated veneer color. The chemical reaction carded out between cellulose or lignin on veneer surface and dye molecules when they met with. The capability of dyeing and discoloration of veneer is improved by control of dyeing technology, especially chemical treatments; being treated by acetylation and maleic anhydride protects dyed wood discoloration.
