采用超高效液相色谱-四极杆-静电场轨道阱高分辨质谱(UPLC-Q-Exactive-Orbitrap MS)对蒲公英根70%乙醇提取物中的化学成分进行快速鉴定.色谱柱采用Hypersil GOLD aQ色谱柱(100 mm×2.1 mm,1.9μm),并以含0.1%甲酸的水溶液(A液)和含0...采用超高效液相色谱-四极杆-静电场轨道阱高分辨质谱(UPLC-Q-Exactive-Orbitrap MS)对蒲公英根70%乙醇提取物中的化学成分进行快速鉴定.色谱柱采用Hypersil GOLD aQ色谱柱(100 mm×2.1 mm,1.9μm),并以含0.1%甲酸的水溶液(A液)和含0.1%甲酸的乙腈(B液)作为流动相.采用以下梯度进行洗脱:0~2 min 5%B液;2~22 min 5%~95%B液;22~27 min 95%B液;27.1~30 min 5%B液.流速为0.3 mL/min,柱温40℃,进样量为5μL.从蒲公英根中鉴定92个化合物,包括6个氨基酸类、17个苯丙素类、3个核苷类、4个黄酮类、4个醛类、2个生物碱类、4个糖类、11个萜类、3个酰胺类、1个香豆素类、23个有机酸类、14个其他类.本研究对蒲公英根的化学成分进行快速鉴定,为进一步研究蒲公英的药效物质基础和资源开发提供依据.展开更多
Lentinus edodes and Auricularia auricula are the first and second largest edible fungi in China with high nutritional and medicinal values.Research about phytochemicals of L.edodes and A.auricula mainly focuses on pol...Lentinus edodes and Auricularia auricula are the first and second largest edible fungi in China with high nutritional and medicinal values.Research about phytochemicals of L.edodes and A.auricula mainly focuses on polysaccharides,other phytochemicals are not fully studied.Phytochemicals of L.edodes and A.auricula were investigated by ultra-high performance liquid chromatography tandem hybrid quadrupole-orbitrap mass spectrometry(UPLC-Q-Exactive-Orbitrap MS).Twenty-three compounds were identified in L.edodes,mainly including amino acids and analogs,flavonoids,nucleosides and analogs,phenols and steroids.Twenty-nine compounds were identified in A.auricula,mainly including amino acids and analogs,nucleosides and analogs,flavonoids and phenols.There are 9 common ingredients of L.edodes and A.auricula,including 4 amino acids and analogs,2 flavonoids,2 nucleosides and analogs and 1 phenol.展开更多
文摘采用超高效液相色谱-四极杆-静电场轨道阱高分辨质谱(UPLC-Q-Exactive-Orbitrap MS)对蒲公英根70%乙醇提取物中的化学成分进行快速鉴定.色谱柱采用Hypersil GOLD aQ色谱柱(100 mm×2.1 mm,1.9μm),并以含0.1%甲酸的水溶液(A液)和含0.1%甲酸的乙腈(B液)作为流动相.采用以下梯度进行洗脱:0~2 min 5%B液;2~22 min 5%~95%B液;22~27 min 95%B液;27.1~30 min 5%B液.流速为0.3 mL/min,柱温40℃,进样量为5μL.从蒲公英根中鉴定92个化合物,包括6个氨基酸类、17个苯丙素类、3个核苷类、4个黄酮类、4个醛类、2个生物碱类、4个糖类、11个萜类、3个酰胺类、1个香豆素类、23个有机酸类、14个其他类.本研究对蒲公英根的化学成分进行快速鉴定,为进一步研究蒲公英的药效物质基础和资源开发提供依据.
基金This work was supported by Major Public Welfare Projects in Henan Province(201300110200)Research on Precision Nutrition and Health Food,Department of Science and Technology of Henan Province(CXJD2021006).
文摘Lentinus edodes and Auricularia auricula are the first and second largest edible fungi in China with high nutritional and medicinal values.Research about phytochemicals of L.edodes and A.auricula mainly focuses on polysaccharides,other phytochemicals are not fully studied.Phytochemicals of L.edodes and A.auricula were investigated by ultra-high performance liquid chromatography tandem hybrid quadrupole-orbitrap mass spectrometry(UPLC-Q-Exactive-Orbitrap MS).Twenty-three compounds were identified in L.edodes,mainly including amino acids and analogs,flavonoids,nucleosides and analogs,phenols and steroids.Twenty-nine compounds were identified in A.auricula,mainly including amino acids and analogs,nucleosides and analogs,flavonoids and phenols.There are 9 common ingredients of L.edodes and A.auricula,including 4 amino acids and analogs,2 flavonoids,2 nucleosides and analogs and 1 phenol.
