[Objectives]To analyze the chemical components of Meconopsis integrifolia(Maxim.)Franch total flavonoids(MITF).[Methods]The chemical components of MITF were identified by UPLC-Q-Exactive Orbitrap MS.The column was per...[Objectives]To analyze the chemical components of Meconopsis integrifolia(Maxim.)Franch total flavonoids(MITF).[Methods]The chemical components of MITF were identified by UPLC-Q-Exactive Orbitrap MS.The column was performed using ACQUITY UPLC HSS T3(100 mm×2.1 mm,1.8μm)with 0.1%formic acid water(A)-acetonitrile(B)with gradient elution at 30℃,injection volume of 2μL,and flow rate of 0.3 mL/min.Electrospray ion source adopted positive and negative ion detection mode with scanning range m/z 100-1000.[Results]A total of 93 compounds were identified from MITF,including 57 flavonoids,25 phenolic acids,9 alkaloids and 2 others.Among them,dimethoxytaxifolin was identified as a possible new compound by SciFinder search,and 67 compounds were first identified in M.integrifolia.[Conclusions]This study provides a scientific foundation for clarifying the material basis of the efficacy of M.integrifolia and improving the quality standards.展开更多
基金Supported by Natural Science Foundation of Sichuan Province(2023NS-FSC0610)the Special Fund of Natural Science for the Central Universities of Southwest Minzu University(ZYN2023071).
文摘[Objectives]To analyze the chemical components of Meconopsis integrifolia(Maxim.)Franch total flavonoids(MITF).[Methods]The chemical components of MITF were identified by UPLC-Q-Exactive Orbitrap MS.The column was performed using ACQUITY UPLC HSS T3(100 mm×2.1 mm,1.8μm)with 0.1%formic acid water(A)-acetonitrile(B)with gradient elution at 30℃,injection volume of 2μL,and flow rate of 0.3 mL/min.Electrospray ion source adopted positive and negative ion detection mode with scanning range m/z 100-1000.[Results]A total of 93 compounds were identified from MITF,including 57 flavonoids,25 phenolic acids,9 alkaloids and 2 others.Among them,dimethoxytaxifolin was identified as a possible new compound by SciFinder search,and 67 compounds were first identified in M.integrifolia.[Conclusions]This study provides a scientific foundation for clarifying the material basis of the efficacy of M.integrifolia and improving the quality standards.
文摘采用超高效液相色谱-四极杆/静电场轨道阱高分辨质谱(ultra-high performance liquid chromatography-quadrupole/exactive Orbitrap mass spectrometry,UPLC-Q-Exactive Orbitrap MS)和质量亏损过滤技术(mass defect filter,MDF)对三叶青黄酮类化合物进行定性分析。通过查阅文献,确定三叶青黄酮类物质母核作为MDF模板,根据取代基的数目和种类确定MDF的质量范围、质量亏损范围,采用UPLC-Q-Exactive Orbitrap MS采集—MDF-Orbitrap Traditional Chinese Medicine Library(OTCML)数据库检索—Mass Frontier验证的鉴定策略,对三叶青乙醇冷浸液所含的黄酮类物质进行定性分析,共鉴定出41种黄酮类化合物,其中19个为首次发现。该方法对于准确识别三叶青黄酮类化合物,进而科学评价三叶青质量、促进三叶青合理开发具有一定的指导意义。
