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UPLC-MS/MS同时测定青钱柳叶中10个成分的含量
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作者 张启云 陈祖德 +3 位作者 姜丽 李冰涛 舒任庚 徐国良 《江西中医药》 2026年第2期71-77,共7页
目的:测定并分析不同产地青钱柳叶中的10个有效成分的含量,为青钱柳的质量评价、开发利用提供基础。方法:13批青钱柳叶经50%乙醇水溶液加热回流提取,以ACE Excel 3 C_(18)-AR(2.1 mm×100 mm,1.7 mm)为色谱柱、0.1%甲酸溶液-乙腈溶... 目的:测定并分析不同产地青钱柳叶中的10个有效成分的含量,为青钱柳的质量评价、开发利用提供基础。方法:13批青钱柳叶经50%乙醇水溶液加热回流提取,以ACE Excel 3 C_(18)-AR(2.1 mm×100 mm,1.7 mm)为色谱柱、0.1%甲酸溶液-乙腈溶液为流动相进行梯度洗脱;流速为0.1 mL/min;柱温为40℃;进样量为1μL;采用电喷雾离子源、多重反应监测模式负离子检测。结果:阿福豆苷、3,5-二咖啡酰奎尼酸、山奈酚、槲皮素-3-O-葡萄糖醛酸苷、杨梅素-3-O-β-D-葡萄糖苷、槲皮素、杨梅素、槲皮苷、山奈酚葡萄糖醛酸苷、金丝桃苷分别在16.25~2 080.00 ng/mL、15.62~2 000.00 ng/mL、6.37~816.00 ng/m L、66.25~8 480.00 ng/m L、6.50~832.00 ng/m L、6.37~816.00 ng/m L、3.06~392.00 ng/m L、15.62~2 000.00 ng/mL、31.25~4 000.00 ng/mL、6.37~816.00 ng/mL范围内线性关系良好,R2≥0.999 0;精密度、重复性、稳定性(24 h)试验的RSD均<3%;平均加样回收率为94.92%~101.11%,RSD值为1.14%~2.60%。13批青钱柳叶中上述10种成分含量范围为84.28~4 304.33μg/g、51.13~3 394.68μg/g、31.43~205.52μg/g、4 493.34~32 623.61μg/g、1.19~131.87μg/g、29.58~277.82μg/g、0.38~10.96μg/g、7.30~1 055.44μg/g、2 559.37~10 418.93μg/g、92.95~1 805.75μg/g。结论:建立的多成分含量测定方法灵敏度高、重复性好,可用于青钱柳中10个成分的含量测定,为青钱柳的质量评价提供参考。 展开更多
关键词 青钱柳 uplc-ms/MS 含量测定 多重反应监测
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基于UPLC-MS/MS分析山西陈醋与恒顺香醋的差异物质
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作者 贾莹莹 秦宇 +3 位作者 周景丽 杨春 李晨 范晓军 《中国调味品》 北大核心 2026年第1期212-219,243,共9页
为了深入研究产地对食醋代谢产物的影响,采用超高效液相色谱-质谱技术,通过非靶向代谢组学分析,对来自山西省太原市的山西陈醋(MJA)与江苏省镇江市的恒顺香醋(HSA)的代谢产物进行了分析。结果显示,两地食醋中共鉴定出1602种化合物,包括... 为了深入研究产地对食醋代谢产物的影响,采用超高效液相色谱-质谱技术,通过非靶向代谢组学分析,对来自山西省太原市的山西陈醋(MJA)与江苏省镇江市的恒顺香醋(HSA)的代谢产物进行了分析。结果显示,两地食醋中共鉴定出1602种化合物,包括脂质、有机酸、有机氮化合物、有机杂环类化合物等多个类别。通过主成分分析与正交偏最小二乘判别分析可以显著区分MJA样品和HSA样品,并筛选出495种具有显著差异的物质。MJA样品中有360种差异物质的相对含量高于HSA样品,其中四甲基吡嗪、褪黑素、核黄素、吲哚等化合物对形成两地食醋的独特风味起到重要作用。代谢通路研究表明,两地食醋在有机杂环类化合物和生物碱及其衍生物的构成上存在显著性差异,这可能是造成两地食醋滋味和品质差异的决定性因素。该研究为鉴别食醋产地提供了重要的理论基础。 展开更多
关键词 uplc-ms/MS 差异物质 多元统计方法 代谢通路
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ELISA法与UPLC-MS/MS法检测水产品中喹诺酮类药物残留的比较分析
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作者 张乐 徐加兵 +2 位作者 孙伟翔 江翰 杨海峰 《中国饲料》 北大核心 2026年第3期156-163,共8页
采用酶联免疫法(ELISA)和超高效液相色谱-串联质谱联用法(UPLC-MS/MS)检测市售6种水产品中14种喹诺酮类药物(QNs)残留的效果,旨在比较两种方法结果的差异性和相关性。其中,UPLC-MS/MS法参考国家标准GB/T 20366-2006进行检测分析,建立14... 采用酶联免疫法(ELISA)和超高效液相色谱-串联质谱联用法(UPLC-MS/MS)检测市售6种水产品中14种喹诺酮类药物(QNs)残留的效果,旨在比较两种方法结果的差异性和相关性。其中,UPLC-MS/MS法参考国家标准GB/T 20366-2006进行检测分析,建立14种QNs药物标准曲线,外标法进行定量分析的结果与ELISA法比较。结果表明:ELISA法检测QNs类药物样品阳性检出率为78.57%,UPLC-MS/MS法检出率为64.29%,环丙沙星最高残留量在黄颡鱼头中为15.76μg/kg,恩诺沙星UPLC-MS/MS法的阳性检出率为64.29%,且最高残留量高达2128.41μg/kg。其中ELISA法样品检出率普遍高于UPLC-MS/MS法,说明ELISA法存在假阳性的情况。鉴于ELISA法分析速度快,样品前处理方法简单等优点,适合用于大批量的样品初筛,UPLC-MS/MS法准确度高,重现性好,可用于ELISA法阳性结果的验证和更精确的药物残留含量测定。本实验为水产品中监测QNs药物残留提供新的检测思路,可结合实际工作情况,选择更快更好的方法,为水产品中用药或水产养殖饲料添加剂的QNs类药物残留检测提供技术支持。 展开更多
关键词 ELISA法 uplc-ms/MS法 喹诺酮类药物 水产品
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硅胶分散固相萃取-UPLC-MS快速检测禽肉和禽蛋中抗球虫药残留
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作者 孟春杨 钟雪 +3 位作者 吴玉田 杨绍群 周贻兵 刘文政 《中国饲料》 北大核心 2026年第3期150-155,共6页
为建立超高效液相色谱串联质谱(UPLC-MS)快速检测禽肉和禽蛋中4种抗球虫药(托曲珠利、托曲珠利砜、地克珠利、尼卡巴嗪)的方法,样品用乙腈提取后,经硅胶分散固相萃取和正己烷脱脂净化,以0.1%甲酸水和甲醇为流动相梯度洗脱(ESI^(-)),用Wa... 为建立超高效液相色谱串联质谱(UPLC-MS)快速检测禽肉和禽蛋中4种抗球虫药(托曲珠利、托曲珠利砜、地克珠利、尼卡巴嗪)的方法,样品用乙腈提取后,经硅胶分散固相萃取和正己烷脱脂净化,以0.1%甲酸水和甲醇为流动相梯度洗脱(ESI^(-)),用Waters ACQUITY UPLC■ BEH C_(18)色谱柱分离,多反应监测(MRM),托曲珠利采用内标法定量,其余3种抗球虫药采用外标法定量。结果表明:4种抗球虫药在0.50~45μg/L的线性范围内,相关系数(r)>0.998,定量限(LOQ)为1.0~3.0μg/kg。3个加标水平下的回收率为81.70%~106.63%,相对标准偏差(RSD)为0.48%~6.76%。本方法简便、快速、准确,可用于禽肉和禽蛋中抗球虫药的定性、定量检测。对购买的66份鸡蛋和66份鸡肉进行检测,鸡蛋的检出率为7.58%,鸡肉的检出率为6.06%。 展开更多
关键词 超高效液相色谱-串联质谱(uplc-ms) 硅胶 抗球虫药 禽肉 禽蛋
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基于UPLC-MS/MS和网络药理学的金针菇酶解物治疗阿尔兹海默症作用机制探究
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作者 郑剑斌 曾婷 赵立娜 《福建轻纺》 2026年第1期26-32,共7页
随着社会老龄化进程的加速,阿尔茨海默病的预防与干预已成为亟待应对的重要问题。文章通过网络药理学和分子对接方法,系统阐明金针菇酶解物(FVEH)治疗阿尔兹海默症(AD)的作用机制,并基于UPLC-MS/MS鉴定FVEH中活性化合物,使用TCMSP等数... 随着社会老龄化进程的加速,阿尔茨海默病的预防与干预已成为亟待应对的重要问题。文章通过网络药理学和分子对接方法,系统阐明金针菇酶解物(FVEH)治疗阿尔兹海默症(AD)的作用机制,并基于UPLC-MS/MS鉴定FVEH中活性化合物,使用TCMSP等数据库筛选其靶点,并通过GeneCards和OMIM获取AD相关靶标,利用STRING数据库构建“疾病-药物-活性成分-关键靶蛋白”相互作用(PPI)网络,预测FVEH的功能和机制。选择PPI网络中排名前10的相互作用靶点(STAT3、BCL2、IL6、MMP9、CASP3、NFKB1、HIF1A、AKT1、IL1B、MMP2)与其对应化合物进行分子对接,研究筛选出113个潜在靶向基因,发现FVEH与调控氧化应激、β-淀粉样蛋白反应、基因表达及免疫信号机制密切相关,提示其通过多组分、多靶点、多通路发挥作用。文章应用网络药理学系统探讨FVEH在AD中的作用,为其作为AD预防和干预的膳食策略提供了理论依据。 展开更多
关键词 金针菇 阿尔兹海默症 网络药理学 uplc-ms/MS
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Research on the Optimal Allocation of Community Elderly Care Service Resources Based on Big Data Technology
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作者 Shuying Li 《Journal of Clinical and Nursing Research》 2026年第1期241-246,共6页
With the accelerating aging process of China’s population,the demand for community elderly care services has shown diversified and personalized characteristics.However,problems such as insufficient total care service... With the accelerating aging process of China’s population,the demand for community elderly care services has shown diversified and personalized characteristics.However,problems such as insufficient total care service resources,uneven distribution,and prominent supply-demand contradictions have seriously affected service quality.Big data technology,with core advantages including data collection,analysis and mining,and accurate prediction,provides a new solution for the allocation of community elderly care service resources.This paper systematically studies the application value of big data technology in the allocation of community elderly care service resources from three aspects:resource allocation efficiency,service accuracy,and management intelligence.Combined with practical needs,it proposes optimal allocation strategies such as building a big data analysis platform and accurately grasping the elderly’s care needs,striving to provide operable path references for the construction of community elderly care service systems,promoting the early realization of the elderly care service goal of“adequate support and proper care for the elderly”,and boosting the high-quality development of China’s elderly care service industry. 展开更多
关键词 Big data technology COMMUNITY Elderly care Service resources
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Tracking a High-Tech Transition--How technology is powering Guangdong’s manufacturing transformation
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作者 HU FAN 《ChinAfrica》 2026年第1期30-32,共3页
The moment a media delegation from the Republic of the Congo arrived at the Othello Kitchenware Museum on 18 November 2025,they were greeted with a vivid show of Guangdong’s industrial strength.Standing before them w... The moment a media delegation from the Republic of the Congo arrived at the Othello Kitchenware Museum on 18 November 2025,they were greeted with a vivid show of Guangdong’s industrial strength.Standing before them was not a typical exhibition hall,but a building shaped like a gleaming stainless-steel cooking pot. 展开更多
关键词 othello kitchenware museum technology industrial strength high tech transition guangdong manufacturing transformation
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Setting the standard! The technology that shapes 40% of the world's denim finishing
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《China Textile》 2026年第1期44-44,共1页
Jeanologia celebrated its 30th anniversary reaffirming the mission it was born with in 1994,to transform the textile industry into a more sustainable,efficient,and human-centered sector.Three decades later,that vision... Jeanologia celebrated its 30th anniversary reaffirming the mission it was born with in 1994,to transform the textile industry into a more sustainable,efficient,and human-centered sector.Three decades later,that vision has become a global reality.Today,more than 40 percent of all jeans produced worldwide are made using technologies developed by the Valencia-based company.What started in a small laboratory in Valencia(Spain),with a pioneering team and a visionary idea,has evolved into a global benchmark in sustainable innovation.From the beginning,Jeanologia believed in technology as a driver of change,introducing solutions that eliminated harmful practices and opened the door to a new,cleaner way of making garments. 展开更多
关键词 technological transformation environmental impact textile industry garment production DENIM jeanologia sustainability innovation
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Reform of Teacher-Machine-Student Interactive Teaching Model of“HarmonyOS Development Technology”Course Based on Congyou Platform
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作者 Yixian Liu Jun Guo +1 位作者 Dongming Chen Zhiliang Zhu 《计算机教育》 2026年第3期158-166,共9页
Aiming at the problems of lagging curriculum,weak practice,and single evaluation in the cultivation of HarmonyOS Development talents,this study constructs a“teacher-machine-student”ternary interactive teaching model... Aiming at the problems of lagging curriculum,weak practice,and single evaluation in the cultivation of HarmonyOS Development talents,this study constructs a“teacher-machine-student”ternary interactive teaching model based on the Congyou platform.Through the building block curriculum system,the HarmonyOS technology stack is decoupled into dynamic capability units,and a multi-disciplinary cross-case library is jointly built with Huawei,which significantly improves the synchronization of teaching content and industrial technology.This paper innovatively designs an AI collaborative teaching system,which employs knowledge graphs to plan learning paths,utilizes virtual equipment clusters to simulate development environments,and establishes a“diagnosis-feedback-enhancement”closed loop through AI-based review,thereby effectively improving students’development efficiency and code reuse rate.A three-dimensional evaluation model integrating task outcomes,process performance,and innovation is constructed,incorporating indicators such as code standardization and an innovation index to strengthen the cultivation of engineering thinking and innovative ability.Furthermore,a data-driven support platform is built to generate student competency profiles,open up the“credit-competency-certification”pathway,promote the transformation of course achievements into contributions to the Huawei ecosystem,and significantly shorten the job adaptation cycle for graduates.The research results provide a replicable paradigm for the cultivation of domestic operating system talents. 展开更多
关键词 Interactive teaching model HarmonyOS development technology Building-block curriculum system Domestic software ecosystem
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Chinese Rural Women's Social Media Use: An Empirical Study Adopting the Technology Acceptance Model
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作者 Zhang Hui Mahadevan Krishnan 《Contemporary Social Sciences》 2026年第1期104-122,共19页
Given the growing importance of social media in digital rural development, this study systematically investigated the influence pathways of social media use among rural women in China, drawing on the Technology Accept... Given the growing importance of social media in digital rural development, this study systematically investigated the influence pathways of social media use among rural women in China, drawing on the Technology Acceptance Model(TAM). Employing quantitative research methods, the study conducted empirical tests based on 367 valid questionnaires using Partial Least Squares Structural Equation Modeling(PLS-SEM) via SmartPLS 4.0 software. Results indicate that significant associations exist between perceived ease of use, perceived usefulness, attitudes toward use, behavioral intention, and actual usage behavior. Specifically, the study finds that rural women's perceived ease of use of social media has a significant and positive influence on both perceived usefulness and attitudes toward use. Perceived usefulness further significantly promotes attitudes toward use and behavioral intention. Moreover, positive attitudes toward usage and strong behavioral intentions were effectively converted into actual social media usage behaviors. This study not only validates the applicability and explanatory power of the TAM model in understanding the digital behavior of Chinese rural women but also provides quantitative evidence for how social media enhances their “digital visibility.” These findings offer practical insights for governments and platform providers to optimize user experiences and strengthen digital skills training. Despite its limitations, including a cross-sectional design and a regional sample, this research holds significant theoretical and practical implications. 展开更多
关键词 Chinese rural women social media technology Acceptance Model(TAM) digital visibility
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Between Themis and Prometheus:a history of the ethics of science and technology
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作者 Jia-Yun Chen Zong-Zhen Wu +7 位作者 Qiang-Yu Xiang Lu Zhang Jia-Lin Diao Ya-Hui Zhang Shen-Shen Yan Kun-Lan Zuo Jian-Liang Huang Huan Liu 《History and Philosophy of Medicine》 2026年第1期49-59,共11页
Background:With the rapid development of modern emerging technologies,the ethical dilemmas and social controversies triggered by scientific and technological activities have become increasingly prominent.How to guide ... Background:With the rapid development of modern emerging technologies,the ethical dilemmas and social controversies triggered by scientific and technological activities have become increasingly prominent.How to guide technology for good and prevent and control technological risks has become an important issue of global concern.Research on science and technology ethics is dedicated to integrating ethical theories into governance practices and constructing ethical models that adapt to the development of the times.Methods:This article systematically reviews the six core approaches of scientific and technological ethics thought,including technological autonomy and political philosophy criticism,responsibility ethics and intergenerational obligations,technological intermediation and the integration of life and the world,ethical principles and normative frameworks,participatory governance and ethical practice innovation,as well as domain-specific ethical norms,thereby constructing an ethical analysis framework applicable to medical technology risks.And cross-analysis was conducted by taking medical events such as gene editing and xenotransplantation as examples.Results:Research shows that a single ethical approach has limitations in addressing complex medical ethical challenges,while the six approaches are complementary and synergistic.By criticizing technological autonomy,establishing a responsibility ethics orientation,setting the bottom line of ethical principles,promoting participatory governance,formulating domain norms,and continuously reflecting on the intermediary nature of technology,a multi-level and dynamically adaptive governance system for scientific and technological ethics can be constructed.Conclusion:The key to addressing contemporary medical ethics challenges lies in the comprehensive application of science and technology ethics theories and the integration of ethical considerations throughout the entire process of scientific and technological research and development.In the future,a governance framework that adapts to the development of new technologies should be established to promote cross-cultural and cross-disciplinary ethical dialogue and public participation,ensuring that scientific and technological innovation always serves the dignity of human life and overall well-being. 展开更多
关键词 ethics of science and technology history of science ethical governance responsible innovation medical ethics
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Teaching Reform of Chinese Medicine Processing Technology Course under the Integration of Technical and Higher Vocational Education
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作者 Lianfang LI 《Medicinal Plant》 2026年第1期72-77,82,共7页
Against the backdrop of integrated development between technical education and higher vocational education,the teaching of Chinese Medicine Processing Technology courses faces new opportunities and challenges.This pap... Against the backdrop of integrated development between technical education and higher vocational education,the teaching of Chinese Medicine Processing Technology courses faces new opportunities and challenges.This paper analyzes the existing problems in the current teaching of Chinese Medicine Processing Technology courses,discusses the necessity of reforming the teaching model under the context of integration,and proposes the construction of a"Dual-Capability Progression,Six-Dimensional Empowerment"teaching model.The aim is to enhance the teaching quality of Chinese Medicine Processing Technology courses and cultivate high-quality skilled talents in Chinese medicine processing who can meet industry demands. 展开更多
关键词 Technical education Higher vocational education Integrated development Chinese Medicine Processing technology Course Teaching reform
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Optical lateral flow immune assay technology for body fluid sensing
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作者 Chen Liu Tianqi Zhao +5 位作者 Jialing Zhou Xiaoyun Hu Dinghao Pan Jinlong Li Wei Li Zhihui Dai 《Chinese Chemical Letters》 2026年第1期106-115,共10页
Detecting biomarkers in body fluids by optical lateral flow immune assay(LFIA) technology provides rapid access to disease information for early diagnosis.LFIA is based on an antigen-antibody reaction and is rapidly b... Detecting biomarkers in body fluids by optical lateral flow immune assay(LFIA) technology provides rapid access to disease information for early diagnosis.LFIA is based on an antigen-antibody reaction and is rapidly becoming the preferred choice of physicians and patients for point-of-care testing due to its simplicity,cost-effectiveness,and rapid detection.Observing the optical signal change from the colloidal gold of the traditional LFIA strip has been widely applied for various biomarkers detection in body fluids.Despite the significant progress,rapid real-time detection of color changes in the colloidal gold by the naked eye still faces many limitations,such as large errors and the inability to quantify and accurately detect.New optical LFIA strip technology has emerged in recent years to extend its application scenarios for achieving quantitative detection such as fluorescence,afterglow,and chemiluminescence.Herein,we summarized the development of optical LFIA technology from single to hyphenated optical signals for biomarkers detection in body fluids from invasive and non-invasive sources.Moreover,the challenge and outlook of optical LFIA strip technology are highlighted to inspire the designing of next-generation diagnostic platforms. 展开更多
关键词 Optical signal Lateral flow immune assay Hyphenated optical technology Body fluid sensing Point-of-care testing
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Application of Whole Slide Imaging Technology in Clinical Pathological Diagnosis
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作者 Zhenjie Wang Jianguo Huai 《Journal of Clinical and Nursing Research》 2026年第1期21-26,共6页
Whole Slide Imaging (WSI) technology, as a revolutionary digital technology in the field of pathology, is gradually changing the traditional clinical pathological diagnosis model. By converting traditional glass patho... Whole Slide Imaging (WSI) technology, as a revolutionary digital technology in the field of pathology, is gradually changing the traditional clinical pathological diagnosis model. By converting traditional glass pathological sections into complete digital images through high-resolution scanning, it provides a new method for pathological diagnosis. Based on this, this paper studies the application of WSI technology in clinical pathological diagnosis, elaborates on its application value, analyzes the current application status, and proposes corresponding application countermeasures, aiming to provide reference for the standardized and popularized development of this technology in clinical pathological diagnosis. 展开更多
关键词 Whole slide imaging(WSI)technology Clinical pathological diagnosis Application value Current status Application countermeasures
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Utilization of a UPLC-MS/MS Approach to Elucidate the Role of ABCB1-Mediated Paclitaxel Resistance in Non-Small Cell Lung Cancer Cells
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作者 Sha Hu Wenjing Wang +6 位作者 Qianfang Hu Rujuan Zheng Qinghe Huang Hui Shi Xinyuan Ding Wenjuan Wang Zengyan Zhu 《Oncology Research》 2026年第2期402-423,共22页
Objectives:Acquired resistance to paclitaxel represents a critical barrier to the effective chemotherapy of non-small cell lung cancer(NSCLC).The present study aimed to elucidate the molecular and pharmacological mech... Objectives:Acquired resistance to paclitaxel represents a critical barrier to the effective chemotherapy of non-small cell lung cancer(NSCLC).The present study aimed to elucidate the molecular and pharmacological mechanisms promoting paclitaxel resistance in NSCLC and to explore potential strategies for overcoming this resistance.Methods:Here,we report an integrated pharmacological and analytical approach to quantify paclitaxel disposition and overcome resistance in a A549/TAX cell model(paclitaxel-resistant A549 cells).Results:Cell counting kit-8(CCK-8)assay,colony formation,and apoptosis assays confirmed that A549/TAX cells exhibited marked resistance to paclitaxel relative to parental A549 cells.Based on transcriptome profiling by RNA sequencing analysis and validation by western blotting assay,we found that the expression of the ATP-binding cassette subfamily B member 1(ABCB1)(the encoded protein is termed P-glycoprotein)was significantly upregulated in resistant cells.By using ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS),we demonstrated that ABCB1 overexpression promotes enhanced efflux of intracellular paclitaxel,thereby lowering its cytotoxic accumulation.Genetic silencing of ABCB1 or pharmacological inhibition with the specific P-glycoprotein modulator elacridar or tariquidar restored intracellular paclitaxel levels,as determined by UPLC-MS/MS,and synergistically decreased cell viability as observed in CCK-8 assay.Conclusion:These findings reveal that the ABCB1-mediated drug efflux is a crucial mechanism underlying paclitaxel resistance in NSCLC cells,with UPLC-MS/MS serving as a sensitive analytical method to detect paclitaxel concentration.Inhibition of ABCB1 is a promising therapeutic strategy to resensitize resistant tumor cells to paclitaxel. 展开更多
关键词 Non-small cell lung cancer(NSCLC) CHEMORESISTANCE PACLITAXEL ATP-binding cassette subfamily B member 1(ABCB1) ultra performance liquid chromatography-tandem mass spectrometry(uplc-ms/MS)
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Defence Technology Aims and Scope
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《Defence Technology(防务技术)》 2026年第2期F0002-F0002,共1页
Defence Technology(ISSN 2214-9147(O);2096-3459(P)),sponsored by China Ordnance Society,is published monthly and aims to become one of the well-known comprehensive journals in the world,which reports on the breakthroug... Defence Technology(ISSN 2214-9147(O);2096-3459(P)),sponsored by China Ordnance Society,is published monthly and aims to become one of the well-known comprehensive journals in the world,which reports on the breakthroughs in defence technology by building up an international academic exchange platform for the defence technology related research.It publishes original research papers having direct bearing on defence,with a balanced coverage on analytical,experimental,numerical simulation and applied investigations.It covers various disciplines of science,technology and engineering. 展开更多
关键词 defence technology applied investigation original research papers analytical methods academic exchange platform breakthroughs experimental methods international academic exchange
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Low-temperature bonding of W90 tungsten heavy alloy using Sn−2Al filler metal by ultrasonic-assisted coating technology
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作者 Xiao-guo SONG Xu-dong ZHANG +3 位作者 Wei FU Xiao-yu TIAN Guang-dong WU Sheng-peng HU 《Transactions of Nonferrous Metals Society of China》 2026年第1期259-272,共14页
The Sn−2Al filler metal was utilized to bond W90 tungsten heavy alloys by the ultrasonic-assisted coating technology in atmospheric environment at 250℃.The effects of ultrasonic power and ultrasonic time on microstru... The Sn−2Al filler metal was utilized to bond W90 tungsten heavy alloys by the ultrasonic-assisted coating technology in atmospheric environment at 250℃.The effects of ultrasonic power and ultrasonic time on microstructure and interfacial strength of Sn−2Al/W90 interface were investigated.The ultrasound improved the wettability of Sn−2Al filler metal on W90 surface.As the ultrasonic power increased and ultrasonic time increased,the size of Al phase in seam decreased.The maximum value of Sn−2Al/W90 interfacial strength reached 30.1 MPa.Based on the acoustic pressure simulation and bubble dynamics,the intensity of cavitation effect was proportional to ultrasonic power.The generated high temperature and high pressure by cavitation effect reached 83799.6 K and 1.26×10^(14) Pa,respectively. 展开更多
关键词 W−Ni−Fe alloy Sn−Al filler metal ultrasonic-assisted coating technology acoustic pressure CAVITATION bubble dynamics
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A review of microfluidic technology for CO_(2)sequestration in saline aquifers
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作者 Yiyan Zhong Qi Li +2 位作者 Wenbin Gao Yiping Wen Yao Zhang 《Journal of Rock Mechanics and Geotechnical Engineering》 2026年第1期810-829,共20页
In recent years,microfluidic technology has emerged as a powerful and innovative tool,attracting significant attention for its ability to provide real-time visualization of CO_(2)flow,mass transfer,and reaction proces... In recent years,microfluidic technology has emerged as a powerful and innovative tool,attracting significant attention for its ability to provide real-time visualization of CO_(2)flow,mass transfer,and reaction processes in porous media.This review examines the application of microfluidic technology in CO_(2)sequestration in saline aquifers,emphasizing the advantages of saline aquifer for geological sequestration,including safety,high storage capacity,stability,and cost-effectiveness.The materials used for microfluidic chips and the design of microchannels are systematically reviewed,offering forward-looking recommendations for chip selection and microchannel characterization in future research on CO_(2)sequestration in saline aquifer.Based on a detailed analysis of advancements in microfluidic technology,this review highlights key findings related to CO_(2)trapping mechanisms,salt precipitation,and CO_(2)-water-rock chemical interactions within saline aquifers.Although microfluidic technology shows great promise in these areas,this review identifies limitations in current studies and outlines future research directions,aiming to promote further innovation and broader application of microfluidic technology in the field of CO_(2)sequestration in saline aquifer. 展开更多
关键词 Microfluidic technology Chip materials Microchannel fabrication CO_(2)trapping mechanisms Salt precipitation CO_(2)-water-rock interaction
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牛肝中28种镇静剂类药物及代谢物残留UPLC-MS/MS检测方法研究 被引量:1
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作者 刘洪斌 时若寒 +2 位作者 李颖 叶子煜 蔡英华 《中国兽医卫生》 2025年第1期29-35,共7页
为了检测养殖和运输环节是否存在非法使用镇静剂类药物,消除动物产品中药物残留隐患,特建立牛肝中28种镇静剂类药物及代谢物残留高效液相色谱-串联质谱检测方法(UPLC-MS/MS)。采用酸性乙腈提取样品,经Captiva EMR-Lipid柱净化,Poroshell... 为了检测养殖和运输环节是否存在非法使用镇静剂类药物,消除动物产品中药物残留隐患,特建立牛肝中28种镇静剂类药物及代谢物残留高效液相色谱-串联质谱检测方法(UPLC-MS/MS)。采用酸性乙腈提取样品,经Captiva EMR-Lipid柱净化,Poroshell 120 EC-C18色谱柱分离。液相选择2 mmol/L乙酸铵和0.1%甲酸的水溶液-乙腈作为流动相进行梯度洗脱,质谱采用正离子多反应监测模式,方法采用基质匹配外标法定量。结果显示,在0.1~200 μg/L浓度范围内,28种镇静剂类药物线性良好,相关系数均在0.995以上,方法的定量限低于1.0 μg/kg。三个浓度(1 μg/kg、2 μg/kg、10 μg/kg)的添加回收实验显示,平均回收率均在62.7%~115.6%之间,批内、批间相对标准偏差(RSD)均小于15%。实验结果表明,该方法的灵敏度高、稳定性好,准确度及精密度满足GB/T 27404-2008中的技术要求,为检测养殖动物滥用镇静剂类药物等违法行为提供一定的技术参考。 展开更多
关键词 牛肝 镇静剂类 uplc-ms/MS 残留 非法药物添加
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基于UPLC-MS/MS、网络药理学、分子对接和体外实验探究左归丸治疗阿尔茨海默病的机制 被引量:1
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作者 王燕 唐亮 +1 位作者 刘瑶 冯星 《中南药学》 2025年第8期2157-2164,共8页
目的基于UPLC-MS/MS和网络药理学探讨左归丸抗阿尔茨海默病(AD)药效物质及作用机制。方法采用UPLC-MS/MS法鉴定左归丸的化学成分;采用网络药理学方法分析左归丸的有效成分、作用靶点及信号通路;采用分子对接技术分析左归丸活性成分与作... 目的基于UPLC-MS/MS和网络药理学探讨左归丸抗阿尔茨海默病(AD)药效物质及作用机制。方法采用UPLC-MS/MS法鉴定左归丸的化学成分;采用网络药理学方法分析左归丸的有效成分、作用靶点及信号通路;采用分子对接技术分析左归丸活性成分与作用靶点的结合情况;噻唑蓝比色法(MTT)测定左归丸对HT22细胞增殖的影响;酶联免疫吸附法(ELISA)检测AD细胞模型的Aβ_(1-42)表达水平;蛋白免疫印迹法检测p-PI3K和p-AKT蛋白表达水平。结果UPLC-MS/MS法共鉴定出501种化合物,通过HERB和SwissADME数据库鉴定出22种有效成分。通过与AD相关的靶点取交集,确定了123个交集靶点。从蛋白质互作网络(PPI)中获得8个核心靶点。GO富集分析结果表明,这些靶点主要与外源刺激、基因表达的正调控、对缺氧的反应有关。KEGG通路分析表明,这些靶点与癌症通路、神经变性-多发性疾病通路、阿尔茨海默病通路和PI3K/AKT信号通路相关。分子对接结果表明,左归丸有效成分与磷脂酰肌醇-3激酶催化亚基α(PIK3CA)、表皮生长因子受体(EGFR)、基质金属蛋白酶9(MMP9)、雷帕霉素靶蛋白(mTOR)和肿瘤坏死因子(TNF)、丝裂原激活蛋白激酶激酶1(MAP2K1)、RAF激酶蛋白(BRAF)具有良好的结合亲和力。体外实验证明,中、高浓度左归丸可显著提升细胞活力,降低HT22细胞Aβ_(1-42)水平(P<0.05),提高p-PI3K和p-AKT蛋白的表达水平(P<0.05)。结论左归丸含有22种有效成分,其可能通过调节PI3K/AKT通路,减少Aβ_(1-42)的产生,进而改善AD。 展开更多
关键词 左归丸 uplc-ms/MS 网络药理 分子对接 阿尔茨海默病
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