Based on multidisciplinary research methods,this study analyzes the mechanisms and potential of Gastrodiae Rhizoma(Tianma)and Uncariae Ramulus Cum Uncis compatibility in treating hypertension.The research confirms tha...Based on multidisciplinary research methods,this study analyzes the mechanisms and potential of Gastrodiae Rhizoma(Tianma)and Uncariae Ramulus Cum Uncis compatibility in treating hypertension.The research confirms that components including rhynchophylline and gastrodin exert antihypertensive effects by inhibiting vascular smooth muscle proliferation and modulating calcium signaling pathways.The combination of these two herbs enhances the bioavailability of core components,synergistically regulates targets such as PTGS2 and NOS3,activates the PPAR signaling pathway,and strengthens vasodilation and antioxidant capacity.Clinical studies demonstrate that Tianma Gouteng Decoction exhibits superior efficacy in improving mitochondrial function and delaying vascular aging compared to single components.This investigation provides scientific evidence for the multi-target antihypertensive effects of traditional Chinese medicine and promotes its modern application in cardiovascular disease treatment.展开更多
OBJECTIVE:To investigate the efficacy of Tianma(Rhizoma Gastrodiae)and Gouteng(Ramulus Uncariae Rhynchophyllae cum Uncis)on cytochrome P450(CYP450)enzyme activities in rats.METHODS:A cocktail strategy was followed to ...OBJECTIVE:To investigate the efficacy of Tianma(Rhizoma Gastrodiae)and Gouteng(Ramulus Uncariae Rhynchophyllae cum Uncis)on cytochrome P450(CYP450)enzyme activities in rats.METHODS:A cocktail strategy was followed to evaluate the influence of Tianma(Rhizoma Gastrodiae)and Gouteng(Ramulus Uncariae Rhynchophyllae cum Uncis)on the activities of CYP450 isoforms(CYP1A2,CYP3A4,CYP2E1,CYP2C19,CYP2C9,CYP2D6),which were determined by changes in the pharmacokinetic parameters of six probe drugs,theophylline,dapsone,chlorzoxazone,omeprazole,tolbutamide and dextromethorphan.Study groups included,Control group(CG),Tianma(Rhizoma Gastrodiae)group(TM),Gouteng(Ramulus Uncariae Rhynchophyllae cum Uncis)group(GT)and Tianma Gouteng(Gastrodia Uncaria)group(TMGT).RESULTS:No significant differences between Tianma(Rhizoma Gastrodiae)and control groups were found.Compared with the control group,in the Gouteng(Ramulus Uncariae Rhynchophyllae cum Uncis)group both the AUC and t1/2 of dapsone and tolbutamide were reduced,whereas the CL(clearance rate)of dapsone and tolbutamide were increased.Compared with the control group,in the Tianma Gouteng group,the AUC and t1/2 of dapsone and tolbutamide were reduced,the CL of dapsone and tolbutamide were increased,and the AUC and t1/2 of chlorzoxazone were increased and the CL of chlorzoxazone was reduced.CONCLUSION:Tianma(Rhizoma Gastrodiae)has no significant effect on the six CYP450 subtypes.The activities of CYP3A4 and CYP2C9 were increased by Gouteng(Ramulus Uncariae Rhynchophyllae cum Uncis).The activities of CYP3A4 and CYP2C9 were increased,whereas the activity of CYP32E1 was reduced by combined Tianma(Rhizoma Gastrodiae)and Gouteng(Ramulus Uncariae Rhynchophyllae cum Uncis).展开更多
[Objectives]To explore the antibacterial activity of Gouteng Foot Bath Concentrated Liquid.[Methods]The inhibitory activity against Staphylococcus aureus,Escherichia coli,Candida albicans,and Trichophyton rubrum was d...[Objectives]To explore the antibacterial activity of Gouteng Foot Bath Concentrated Liquid.[Methods]The inhibitory activity against Staphylococcus aureus,Escherichia coli,Candida albicans,and Trichophyton rubrum was determined using the mycelial growth rate method.The inhibitory effects of different concentrations of Gouteng Concentrated Liquid on these four microbial strains were analyzed.[Results]Gouteng Foot Bath Concentrated Liquid showed certain antibacterial activity against the above bacteria,and the antibacterial activity against S.aureus,T.rubrum and C.albicans was more significant.[Conclusions]This study provides experimental evidence supporting the application of Gouteng(Uncariae Ramulus Cum Uncis)in antimicrobial foot care products.展开更多
文摘Based on multidisciplinary research methods,this study analyzes the mechanisms and potential of Gastrodiae Rhizoma(Tianma)and Uncariae Ramulus Cum Uncis compatibility in treating hypertension.The research confirms that components including rhynchophylline and gastrodin exert antihypertensive effects by inhibiting vascular smooth muscle proliferation and modulating calcium signaling pathways.The combination of these two herbs enhances the bioavailability of core components,synergistically regulates targets such as PTGS2 and NOS3,activates the PPAR signaling pathway,and strengthens vasodilation and antioxidant capacity.Clinical studies demonstrate that Tianma Gouteng Decoction exhibits superior efficacy in improving mitochondrial function and delaying vascular aging compared to single components.This investigation provides scientific evidence for the multi-target antihypertensive effects of traditional Chinese medicine and promotes its modern application in cardiovascular disease treatment.
基金Supported by the Science and Technology Department of Sichuan Province[Project Name:Study on the Molecular Pharmacokinetic of Tianma(Rhizoma Gastrodiae)and Gouteng(Ramulus Uncariae Rhynchophyllae cum Uncis)in Liver Uptake and Elimination of Upper Hyperactivity of Liver-yang,No.2018FZ0096]and the Fundamental Research Funds for the Central Universities(Study on Pharmacodynamics and in Vivo Process of Chinese Herbal Compound in Treatment of ARDS Caused by Covid-19 Syndrome)。
文摘OBJECTIVE:To investigate the efficacy of Tianma(Rhizoma Gastrodiae)and Gouteng(Ramulus Uncariae Rhynchophyllae cum Uncis)on cytochrome P450(CYP450)enzyme activities in rats.METHODS:A cocktail strategy was followed to evaluate the influence of Tianma(Rhizoma Gastrodiae)and Gouteng(Ramulus Uncariae Rhynchophyllae cum Uncis)on the activities of CYP450 isoforms(CYP1A2,CYP3A4,CYP2E1,CYP2C19,CYP2C9,CYP2D6),which were determined by changes in the pharmacokinetic parameters of six probe drugs,theophylline,dapsone,chlorzoxazone,omeprazole,tolbutamide and dextromethorphan.Study groups included,Control group(CG),Tianma(Rhizoma Gastrodiae)group(TM),Gouteng(Ramulus Uncariae Rhynchophyllae cum Uncis)group(GT)and Tianma Gouteng(Gastrodia Uncaria)group(TMGT).RESULTS:No significant differences between Tianma(Rhizoma Gastrodiae)and control groups were found.Compared with the control group,in the Gouteng(Ramulus Uncariae Rhynchophyllae cum Uncis)group both the AUC and t1/2 of dapsone and tolbutamide were reduced,whereas the CL(clearance rate)of dapsone and tolbutamide were increased.Compared with the control group,in the Tianma Gouteng group,the AUC and t1/2 of dapsone and tolbutamide were reduced,the CL of dapsone and tolbutamide were increased,and the AUC and t1/2 of chlorzoxazone were increased and the CL of chlorzoxazone was reduced.CONCLUSION:Tianma(Rhizoma Gastrodiae)has no significant effect on the six CYP450 subtypes.The activities of CYP3A4 and CYP2C9 were increased by Gouteng(Ramulus Uncariae Rhynchophyllae cum Uncis).The activities of CYP3A4 and CYP2C9 were increased,whereas the activity of CYP32E1 was reduced by combined Tianma(Rhizoma Gastrodiae)and Gouteng(Ramulus Uncariae Rhynchophyllae cum Uncis).
基金Supported by Guizhou Province Science and Technology Planning Program for Industrial Enterprises above Designated Size Without R&D Activities(QKTY[2024]No.456).
文摘[Objectives]To explore the antibacterial activity of Gouteng Foot Bath Concentrated Liquid.[Methods]The inhibitory activity against Staphylococcus aureus,Escherichia coli,Candida albicans,and Trichophyton rubrum was determined using the mycelial growth rate method.The inhibitory effects of different concentrations of Gouteng Concentrated Liquid on these four microbial strains were analyzed.[Results]Gouteng Foot Bath Concentrated Liquid showed certain antibacterial activity against the above bacteria,and the antibacterial activity against S.aureus,T.rubrum and C.albicans was more significant.[Conclusions]This study provides experimental evidence supporting the application of Gouteng(Uncariae Ramulus Cum Uncis)in antimicrobial foot care products.
