Based on multidisciplinary research methods,this study analyzes the mechanisms and potential of Gastrodiae Rhizoma(Tianma)and Uncariae Ramulus Cum Uncis compatibility in treating hypertension.The research confirms tha...Based on multidisciplinary research methods,this study analyzes the mechanisms and potential of Gastrodiae Rhizoma(Tianma)and Uncariae Ramulus Cum Uncis compatibility in treating hypertension.The research confirms that components including rhynchophylline and gastrodin exert antihypertensive effects by inhibiting vascular smooth muscle proliferation and modulating calcium signaling pathways.The combination of these two herbs enhances the bioavailability of core components,synergistically regulates targets such as PTGS2 and NOS3,activates the PPAR signaling pathway,and strengthens vasodilation and antioxidant capacity.Clinical studies demonstrate that Tianma Gouteng Decoction exhibits superior efficacy in improving mitochondrial function and delaying vascular aging compared to single components.This investigation provides scientific evidence for the multi-target antihypertensive effects of traditional Chinese medicine and promotes its modern application in cardiovascular disease treatment.展开更多
OBJECTIVE:To investigate the efficacy of Tianma(Rhizoma Gastrodiae)and Gouteng(Ramulus Uncariae Rhynchophyllae cum Uncis)on cytochrome P450(CYP450)enzyme activities in rats.METHODS:A cocktail strategy was followed to ...OBJECTIVE:To investigate the efficacy of Tianma(Rhizoma Gastrodiae)and Gouteng(Ramulus Uncariae Rhynchophyllae cum Uncis)on cytochrome P450(CYP450)enzyme activities in rats.METHODS:A cocktail strategy was followed to evaluate the influence of Tianma(Rhizoma Gastrodiae)and Gouteng(Ramulus Uncariae Rhynchophyllae cum Uncis)on the activities of CYP450 isoforms(CYP1A2,CYP3A4,CYP2E1,CYP2C19,CYP2C9,CYP2D6),which were determined by changes in the pharmacokinetic parameters of six probe drugs,theophylline,dapsone,chlorzoxazone,omeprazole,tolbutamide and dextromethorphan.Study groups included,Control group(CG),Tianma(Rhizoma Gastrodiae)group(TM),Gouteng(Ramulus Uncariae Rhynchophyllae cum Uncis)group(GT)and Tianma Gouteng(Gastrodia Uncaria)group(TMGT).RESULTS:No significant differences between Tianma(Rhizoma Gastrodiae)and control groups were found.Compared with the control group,in the Gouteng(Ramulus Uncariae Rhynchophyllae cum Uncis)group both the AUC and t1/2 of dapsone and tolbutamide were reduced,whereas the CL(clearance rate)of dapsone and tolbutamide were increased.Compared with the control group,in the Tianma Gouteng group,the AUC and t1/2 of dapsone and tolbutamide were reduced,the CL of dapsone and tolbutamide were increased,and the AUC and t1/2 of chlorzoxazone were increased and the CL of chlorzoxazone was reduced.CONCLUSION:Tianma(Rhizoma Gastrodiae)has no significant effect on the six CYP450 subtypes.The activities of CYP3A4 and CYP2C9 were increased by Gouteng(Ramulus Uncariae Rhynchophyllae cum Uncis).The activities of CYP3A4 and CYP2C9 were increased,whereas the activity of CYP32E1 was reduced by combined Tianma(Rhizoma Gastrodiae)and Gouteng(Ramulus Uncariae Rhynchophyllae cum Uncis).展开更多
目的从文献计量学和全球专利的角度对钩藤的研究动态与焦点进行全面分析,为未来钩藤的临床应用和深入研究提供前瞻参考。方法以钩藤为关键词检索中国知网(CNKI)、万方(Wanfang)、维普(VIP)、Web of Science(WOS)4个数据库,将文献导入Not...目的从文献计量学和全球专利的角度对钩藤的研究动态与焦点进行全面分析,为未来钩藤的临床应用和深入研究提供前瞻参考。方法以钩藤为关键词检索中国知网(CNKI)、万方(Wanfang)、维普(VIP)、Web of Science(WOS)4个数据库,将文献导入NoteExpress文献管理软件中进行查重和筛选。利用Excel、Origin、CiteSpace、VOSviewer等软件,从发文趋势与国家分布、发文机构、发文期刊、发文作者、关键词等维度对国内外的钩藤研究现状与发展趋势进行可视化分析。通过Incopat专利数据库从专利申请趋势、全球地域分布、专利申请人及专利技术领域等方面检索并分析钩藤相关全球专利申请情况。结果共检索到符合标准的中文文献1713篇,英文文献594篇。发文国家以中国为主,国际上对钩藤的关注度也显著提升。钩藤领域的核心团队已初步形成,但机构间合作有待加强。近年来钩藤领域中英文文献研究热点存在交叉现象,均聚焦于钩藤的药理活性成分、作用机制,中文文献聚焦天麻钩藤饮及钩藤用于高血压的治疗及调控机制与活性成分;英文文献侧重于植物化学、抗炎抗氧化作用及神经疾病的潜在疗效。基于Incopat专利数据库检索经初步筛选得到8048件专利,实施同族合并操作后,保留专利6251项,其中国内专利数量为5461,占比87.36%,占据主导地位。结论钩藤领域研究呈现出不断拓展与深化的态势,整体上正从传统中医药实践转向多学科协同创新与分子水平机制深入解析的发展路径。中国虽然在钩藤专利的国际申请中占据主导地位,却存在关键弊端:专利失效规模较大、转化实施程度有限且市场拓展乏力。展开更多
[Objectives]To explore the antibacterial activity of Gouteng Foot Bath Concentrated Liquid.[Methods]The inhibitory activity against Staphylococcus aureus,Escherichia coli,Candida albicans,and Trichophyton rubrum was d...[Objectives]To explore the antibacterial activity of Gouteng Foot Bath Concentrated Liquid.[Methods]The inhibitory activity against Staphylococcus aureus,Escherichia coli,Candida albicans,and Trichophyton rubrum was determined using the mycelial growth rate method.The inhibitory effects of different concentrations of Gouteng Concentrated Liquid on these four microbial strains were analyzed.[Results]Gouteng Foot Bath Concentrated Liquid showed certain antibacterial activity against the above bacteria,and the antibacterial activity against S.aureus,T.rubrum and C.albicans was more significant.[Conclusions]This study provides experimental evidence supporting the application of Gouteng(Uncariae Ramulus Cum Uncis)in antimicrobial foot care products.展开更多
文摘Based on multidisciplinary research methods,this study analyzes the mechanisms and potential of Gastrodiae Rhizoma(Tianma)and Uncariae Ramulus Cum Uncis compatibility in treating hypertension.The research confirms that components including rhynchophylline and gastrodin exert antihypertensive effects by inhibiting vascular smooth muscle proliferation and modulating calcium signaling pathways.The combination of these two herbs enhances the bioavailability of core components,synergistically regulates targets such as PTGS2 and NOS3,activates the PPAR signaling pathway,and strengthens vasodilation and antioxidant capacity.Clinical studies demonstrate that Tianma Gouteng Decoction exhibits superior efficacy in improving mitochondrial function and delaying vascular aging compared to single components.This investigation provides scientific evidence for the multi-target antihypertensive effects of traditional Chinese medicine and promotes its modern application in cardiovascular disease treatment.
基金Supported by the Science and Technology Department of Sichuan Province[Project Name:Study on the Molecular Pharmacokinetic of Tianma(Rhizoma Gastrodiae)and Gouteng(Ramulus Uncariae Rhynchophyllae cum Uncis)in Liver Uptake and Elimination of Upper Hyperactivity of Liver-yang,No.2018FZ0096]and the Fundamental Research Funds for the Central Universities(Study on Pharmacodynamics and in Vivo Process of Chinese Herbal Compound in Treatment of ARDS Caused by Covid-19 Syndrome)。
文摘OBJECTIVE:To investigate the efficacy of Tianma(Rhizoma Gastrodiae)and Gouteng(Ramulus Uncariae Rhynchophyllae cum Uncis)on cytochrome P450(CYP450)enzyme activities in rats.METHODS:A cocktail strategy was followed to evaluate the influence of Tianma(Rhizoma Gastrodiae)and Gouteng(Ramulus Uncariae Rhynchophyllae cum Uncis)on the activities of CYP450 isoforms(CYP1A2,CYP3A4,CYP2E1,CYP2C19,CYP2C9,CYP2D6),which were determined by changes in the pharmacokinetic parameters of six probe drugs,theophylline,dapsone,chlorzoxazone,omeprazole,tolbutamide and dextromethorphan.Study groups included,Control group(CG),Tianma(Rhizoma Gastrodiae)group(TM),Gouteng(Ramulus Uncariae Rhynchophyllae cum Uncis)group(GT)and Tianma Gouteng(Gastrodia Uncaria)group(TMGT).RESULTS:No significant differences between Tianma(Rhizoma Gastrodiae)and control groups were found.Compared with the control group,in the Gouteng(Ramulus Uncariae Rhynchophyllae cum Uncis)group both the AUC and t1/2 of dapsone and tolbutamide were reduced,whereas the CL(clearance rate)of dapsone and tolbutamide were increased.Compared with the control group,in the Tianma Gouteng group,the AUC and t1/2 of dapsone and tolbutamide were reduced,the CL of dapsone and tolbutamide were increased,and the AUC and t1/2 of chlorzoxazone were increased and the CL of chlorzoxazone was reduced.CONCLUSION:Tianma(Rhizoma Gastrodiae)has no significant effect on the six CYP450 subtypes.The activities of CYP3A4 and CYP2C9 were increased by Gouteng(Ramulus Uncariae Rhynchophyllae cum Uncis).The activities of CYP3A4 and CYP2C9 were increased,whereas the activity of CYP32E1 was reduced by combined Tianma(Rhizoma Gastrodiae)and Gouteng(Ramulus Uncariae Rhynchophyllae cum Uncis).
文摘目的从文献计量学和全球专利的角度对钩藤的研究动态与焦点进行全面分析,为未来钩藤的临床应用和深入研究提供前瞻参考。方法以钩藤为关键词检索中国知网(CNKI)、万方(Wanfang)、维普(VIP)、Web of Science(WOS)4个数据库,将文献导入NoteExpress文献管理软件中进行查重和筛选。利用Excel、Origin、CiteSpace、VOSviewer等软件,从发文趋势与国家分布、发文机构、发文期刊、发文作者、关键词等维度对国内外的钩藤研究现状与发展趋势进行可视化分析。通过Incopat专利数据库从专利申请趋势、全球地域分布、专利申请人及专利技术领域等方面检索并分析钩藤相关全球专利申请情况。结果共检索到符合标准的中文文献1713篇,英文文献594篇。发文国家以中国为主,国际上对钩藤的关注度也显著提升。钩藤领域的核心团队已初步形成,但机构间合作有待加强。近年来钩藤领域中英文文献研究热点存在交叉现象,均聚焦于钩藤的药理活性成分、作用机制,中文文献聚焦天麻钩藤饮及钩藤用于高血压的治疗及调控机制与活性成分;英文文献侧重于植物化学、抗炎抗氧化作用及神经疾病的潜在疗效。基于Incopat专利数据库检索经初步筛选得到8048件专利,实施同族合并操作后,保留专利6251项,其中国内专利数量为5461,占比87.36%,占据主导地位。结论钩藤领域研究呈现出不断拓展与深化的态势,整体上正从传统中医药实践转向多学科协同创新与分子水平机制深入解析的发展路径。中国虽然在钩藤专利的国际申请中占据主导地位,却存在关键弊端:专利失效规模较大、转化实施程度有限且市场拓展乏力。
基金Supported by Guizhou Province Science and Technology Planning Program for Industrial Enterprises above Designated Size Without R&D Activities(QKTY[2024]No.456).
文摘[Objectives]To explore the antibacterial activity of Gouteng Foot Bath Concentrated Liquid.[Methods]The inhibitory activity against Staphylococcus aureus,Escherichia coli,Candida albicans,and Trichophyton rubrum was determined using the mycelial growth rate method.The inhibitory effects of different concentrations of Gouteng Concentrated Liquid on these four microbial strains were analyzed.[Results]Gouteng Foot Bath Concentrated Liquid showed certain antibacterial activity against the above bacteria,and the antibacterial activity against S.aureus,T.rubrum and C.albicans was more significant.[Conclusions]This study provides experimental evidence supporting the application of Gouteng(Uncariae Ramulus Cum Uncis)in antimicrobial foot care products.
