伪狂犬病病毒(PRV)是一种对猪和其他哺乳动物造成严重危害的病原体,它能够引发宿主的系统性炎症反应和炎症损伤。尽管已知PRV感染与炎性小体的激活和焦亡有关,但PRV促进炎性小体激活的具体分子机制尚不清楚。2024年9月24日,西北农林科...伪狂犬病病毒(PRV)是一种对猪和其他哺乳动物造成严重危害的病原体,它能够引发宿主的系统性炎症反应和炎症损伤。尽管已知PRV感染与炎性小体的激活和焦亡有关,但PRV促进炎性小体激活的具体分子机制尚不清楚。2024年9月24日,西北农林科技大学研究团队在PLoS Pathogens在线发表了题为“Pseudorabies virus UL4 protein promotes the ASC-dependent inflammasome activation and pyroptosis to exacerbate inflammation”的最新研究成果,鉴定出PRV非结构蛋白UL4的关键功能,揭示其如何增强ASC依赖性的炎性小体激活,促进焦亡,并加剧炎症反应。这一研究成果对于开发新的疫苗和治疗策略具有重要意义。展开更多
Duck enteritis virus(DEV) is a herpesvirus that causes an acute,contagious and fatal disease. In the present article,the DEV UL4 gene was cloned and sequenced from a vaccine virus. A degenerate oligonucleotide primer ...Duck enteritis virus(DEV) is a herpesvirus that causes an acute,contagious and fatal disease. In the present article,the DEV UL4 gene was cloned and sequenced from a vaccine virus. A degenerate oligonucleotide primer for the consensus site of herpesvirus UL3 gene and a specific primer located in UL5 were used in the polymerase chain reaction(PCR) to amplify a DNA product 2 086 bp in size. DNA sequence analysis revealed that a 714 bp open reading frame(ORF) of DEV encoding a 237 amino acid polypeptide is homologous to the family of herpesvirus UL4 proteins and therefore has been characterized as a DEV UL4 gene. Alignment of the DEV UL4 protein sequence with those of other alphaherpesviruses showed that 10 amino acid residues are completely conserved. Phylogenetic tree analysis showed that the seventeen alphaherpesviruses viruses analyzed were classified into four large groups,and the duck enteritis virus branched separately,closely related to the Mardiviruses group comprising Gallid herpesvirus 2(GaHV-2) ,Gallid herpesvirus 3(GaHV-3) and Meleagrid herpesvirus 1(MeHV-1) . The present study showed that the evolutionary relationship of the UL4 protein could be used for classification of alphaherpesviruses.展开更多
The function of the herpes simplex virus type 1 （HSV-1） UL4 protein is still elusive. Our objective is to investigate the subcellular transport mechanism of the UL4 protein. In this study, fluorescence microscopy wa...The function of the herpes simplex virus type 1 （HSV-1） UL4 protein is still elusive. Our objective is to investigate the subcellular transport mechanism of the UL4 protein. In this study, fluorescence microscopy was employed to investigate the subcellular localization of UL4 and characterize the transport mechanism in living cells. By constructing a series of deletion mutants fused with enhanced yellow fluorescent protein （EYFP）, the nuclear export signals （NES） of UL4 were for the first time mapped to amino acid residues 178 to 186. In addition, the N-terminal 19 amino acids are identified to be required for the granule-like cytoplasmic pattem of UL4. Furthermore, the UL4 protein was demonstrated to be exported to the cytoplasm through the NES in a chromosomal region maintenance 1 （CRM1）-dependent manner involving RanGTP hydrolysis展开更多
文摘伪狂犬病病毒(PRV)是一种对猪和其他哺乳动物造成严重危害的病原体,它能够引发宿主的系统性炎症反应和炎症损伤。尽管已知PRV感染与炎性小体的激活和焦亡有关,但PRV促进炎性小体激活的具体分子机制尚不清楚。2024年9月24日,西北农林科技大学研究团队在PLoS Pathogens在线发表了题为“Pseudorabies virus UL4 protein promotes the ASC-dependent inflammasome activation and pyroptosis to exacerbate inflammation”的最新研究成果,鉴定出PRV非结构蛋白UL4的关键功能,揭示其如何增强ASC依赖性的炎性小体激活,促进焦亡,并加剧炎症反应。这一研究成果对于开发新的疫苗和治疗策略具有重要意义。
文摘Duck enteritis virus(DEV) is a herpesvirus that causes an acute,contagious and fatal disease. In the present article,the DEV UL4 gene was cloned and sequenced from a vaccine virus. A degenerate oligonucleotide primer for the consensus site of herpesvirus UL3 gene and a specific primer located in UL5 were used in the polymerase chain reaction(PCR) to amplify a DNA product 2 086 bp in size. DNA sequence analysis revealed that a 714 bp open reading frame(ORF) of DEV encoding a 237 amino acid polypeptide is homologous to the family of herpesvirus UL4 proteins and therefore has been characterized as a DEV UL4 gene. Alignment of the DEV UL4 protein sequence with those of other alphaherpesviruses showed that 10 amino acid residues are completely conserved. Phylogenetic tree analysis showed that the seventeen alphaherpesviruses viruses analyzed were classified into four large groups,and the duck enteritis virus branched separately,closely related to the Mardiviruses group comprising Gallid herpesvirus 2(GaHV-2) ,Gallid herpesvirus 3(GaHV-3) and Meleagrid herpesvirus 1(MeHV-1) . The present study showed that the evolutionary relationship of the UL4 protein could be used for classification of alphaherpesviruses.
基金the Major State Basic Research Development Program of China(2010CB530105 and 2011CB504802)the National Natural Science Foundation of China(30900059,30870120 and 81000736)the Start-up Fund of the Hundred Talents Program of the Chinese Academy of Sciences(20071010-141)
文摘The function of the herpes simplex virus type 1 （HSV-1） UL4 protein is still elusive. Our objective is to investigate the subcellular transport mechanism of the UL4 protein. In this study, fluorescence microscopy was employed to investigate the subcellular localization of UL4 and characterize the transport mechanism in living cells. By constructing a series of deletion mutants fused with enhanced yellow fluorescent protein （EYFP）, the nuclear export signals （NES） of UL4 were for the first time mapped to amino acid residues 178 to 186. In addition, the N-terminal 19 amino acids are identified to be required for the granule-like cytoplasmic pattem of UL4. Furthermore, the UL4 protein was demonstrated to be exported to the cytoplasm through the NES in a chromosomal region maintenance 1 （CRM1）-dependent manner involving RanGTP hydrolysis
