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遗传减毒伯氏疟原虫ANKA株的构建及其免疫保护效应
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作者 丁艳 谭章平 +2 位作者 卢潇 徐文岳 付雍 《热带医学杂志》 CAS 2016年第7期821-824,835,共5页
目的构建遗传减毒伯氏疟原虫ANKA株(Plasmodium berghei ANKA,P.b ANKA),并用此减毒疟原虫株免疫C57BL/6J小鼠观察免疫保护效果。方法分别扩增UIS3基因编码序列两端的非编码区5′UTR和3′UTR及用于筛选的抗性标记h DHFR,然后利用融合PC... 目的构建遗传减毒伯氏疟原虫ANKA株(Plasmodium berghei ANKA,P.b ANKA),并用此减毒疟原虫株免疫C57BL/6J小鼠观察免疫保护效果。方法分别扩增UIS3基因编码序列两端的非编码区5′UTR和3′UTR及用于筛选的抗性标记h DHFR,然后利用融合PCR原理,扩增全长同源重组片段(5′UTR+h DHFR+3′UTR),最后常规PCR扩增获得大量线性化同源重组片段并纯化。体外培养富集伯氏疟原虫ANKA株的成熟裂殖体,将线性化同源重组片段通过电转的方式导入裂殖体中并接种到小鼠体内,再对转化后的疟原虫进行筛选和鉴定,最后用构建成功的减毒伯氏疟原虫ANKA株免疫小鼠并攻击,观察减毒株的免疫保护效力。结果成功扩增3个独立片段5′UTR、h DHFR和3′UTR,长度分别为798、1 628和759 bp,后经融合PCR反应形成全长重组片段5′UTR+h DHFR+3′UTR,长度为3 185 bp。转化至裂殖体后经筛选鉴定获得遗传减毒伯氏疟原虫ANKA株,将此遗传减毒株免疫小鼠后攻击,小鼠红内期原虫率为0%,脑型疟发生率为0%,存活率为100%,可使其完全抵御野生型疟原虫感染。结论成功构建遗传减毒伯氏疟原虫ANKA株,此减毒株对小鼠的免疫保护率为100%,此模型的建立可为研究红前期疫苗免疫保护机制奠定基础。 展开更多
关键词 伯氏疟原虫ANKA株 遗传减毒 uis3 免疫攻击 保护效率
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番茄单向不亲和基因UI3a的精细定位
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作者 宋丽华 崔丽朋 +4 位作者 王孝宣 高建昌 国艳梅 黄泽军 杜永臣 《园艺学报》 CAS CSCD 北大核心 2016年第7期1275-1285,共11页
单向不亲和对于研究种间花粉不亲和及自交不亲和机制有重要意义。以潘那利番茄‘LA0716’渐渗系群体中的单向不亲和株系为主要材料,通过花粉萌发试验,正、反交试验和花粉管在雌蕊中的伸长研究,发现单向不亲和株系的花粉活力正常,其花粉... 单向不亲和对于研究种间花粉不亲和及自交不亲和机制有重要意义。以潘那利番茄‘LA0716’渐渗系群体中的单向不亲和株系为主要材料,通过花粉萌发试验,正、反交试验和花粉管在雌蕊中的伸长研究,发现单向不亲和株系的花粉活力正常,其花粉与普通栽培番茄杂交可正常结实,但是其雌蕊不能接受自身或栽培番茄的花粉。然而,将秘鲁番茄‘LA3858’的花粉授予单向不亲和株系,花粉能够正常萌发,花粉管正常伸长和受精,但是杂交种子败育。利用20个分子标记对BC5S3群体的1 000株植株进行遗传分析,结合表型鉴定结果,将单向不亲和基因UI3a精细定位于第3号染色体上分子标记C2_At3g10220和1k-1294之间,物理距离为349 kb,该区域编码37个基因。 展开更多
关键词 番茄 单向不亲和 UI3a基因 精细定位
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Efficient and Flexible 3D Virtual Desktop System in the Windows Environment 被引量:1
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作者 Seungpyo Hong Donsu Lee Sangjun Lee 《Journal of Measurement Science and Instrumentation》 CAS 2010年第S1期178-181,共4页
As personal computers have been recently improved in performance,a multi-tasking of running several applications at the same time has been generalized. Since only one task space is provided for performing such tasks i... As personal computers have been recently improved in performance,a multi-tasking of running several applications at the same time has been generalized. Since only one task space is provided for performing such tasks in the Windows operating system,a number of computer users feel inconvenience.In this paper,we suggest a 3D Virtual Desktop System which can show a process of switching task spaces in 3D motion using DirectX. Our system aims to make up for the weak points in the Windows operating system,and to provide a computer user with more spaces for performing several tasks as well as the intuitive UI(User Interface),such as Compiz of Linux. 展开更多
关键词 3D Virtual Desktop 3D Desktop Virtual Desktop 3D UI
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Facile preparation of nano-g-C_(3)N_(4)/UiO-66-NH_(2) composite as sorbent for high-efficient extraction and preconcentration of food colorants prior to HPLC analysis 被引量:6
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作者 Xiaowan Zhang Yixin Yang +5 位作者 Peige Qin Lizhen Han Wenli Zhu Shaofeng Duan Minghua Lu Zongwei Cai 《Chinese Chemical Letters》 SCIE CAS CSCD 2022年第2期903-906,共4页
In this work,the nano-g-C_(3)N_(4)/Ui O-66-NH_(2)composite was prepared by one-step solvothermal method.The as-prepared composite was characterized by scanning electron microscopy,Brunner-Emmet-Teller measurement,ener... In this work,the nano-g-C_(3)N_(4)/Ui O-66-NH_(2)composite was prepared by one-step solvothermal method.The as-prepared composite was characterized by scanning electron microscopy,Brunner-Emmet-Teller measurement,energy dispersive spectrometer,X-ray diffraction,and Fourier transform infrared spectroscopy.By using nano-g-C_(3)N_(4)/Ui O-66-NH_(2)composite as sorbent,a dispersive solid-phase extraction coupled with high-performance liquid chromatography was developed to sensitive analysis of food colorants including tartrazine,amaranth,carmine,sunset yellow,allura red and bright blue.The experiment parameters including the amount of sorbent,adsorption time,the p H of adsorption solution,desorption time,desorption solvent,the p H of desorption solution as well as the proportion between desorption solvent and buffer solvent were investigated.Under the optimized conditions,the limits of detection(S/N=3) and limits of quantitation (S/N=10) were determined in the ranges of 0.08-0.8 and 0.2-2.0 ng/m L,respectively.With the developed sample pretreatment method,carmine and brilliant blue were determined from blueberry juice by HPLC-DAD.The contents were calculated as 1.53μg/m L and0.17μg/mL,respectively. 展开更多
关键词 Nano-g-C_(3)N_(4)/Ui O-66-NH_(2)composite Metal-organic frameworks Dispersive solid-phase extraction Sample pretreatment Food colorants Food additives High-performance liquid chromatography
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一种汽车3D UI中带光影贴图车模的制作方法
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作者 杨念祖 邢鱼弟 《汽车与驾驶维修》 2023年第12期71-73,共3页
汽车界面经历了从机械按键到液晶大屏的演进,最新车型采用3D UI和智能驾辅技术,打造沉浸式智能体验。本文详述了3D UI人机交互设计中的车模数据和光影效果优化方法,强调多边形模型在流畅运行和光影效果方面的关键作用。在车模数据量和... 汽车界面经历了从机械按键到液晶大屏的演进,最新车型采用3D UI和智能驾辅技术,打造沉浸式智能体验。本文详述了3D UI人机交互设计中的车模数据和光影效果优化方法,强调多边形模型在流畅运行和光影效果方面的关键作用。在车模数据量和光影效果优化中,需注意调整精度、贴图,平衡效果和效率。未来,随着车载系统算力提升,车辆有望实现更复杂的3D模型处理,为驾驶者和乘客带来更真实、沉浸的视觉体验。 展开更多
关键词 NURBS曲面 Polygon多边形 3D UI OpenGL引擎 UV展开
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