Ultrasonic-Assisted Grinding(UAG)is a novel manufacturing technology that shows promising promise for use in processing Ceramic Matrix Composites(CMCs).Nevertheless,analyzing the material removal process of CMCs with ...Ultrasonic-Assisted Grinding(UAG)is a novel manufacturing technology that shows promising promise for use in processing Ceramic Matrix Composites(CMCs).Nevertheless,analyzing the material removal process of CMCs with multidirectional structure during UAG is challenging,impeding the progress and improvement of the UAG process.This work examined the impact of ultrasonic vibration on the dynamic mechanical characteristics during processing.Additionally,we experimentally elucidated the material removal mechanism of CMCs during the scratching process under the influence of vertical vibration.The results indicate that the introduction of ultrasonic vibration causes a strain rate effect,resulting in a modification of the material removal mechanism,subsequently impacting the processing quality.Ultrasonic vibration increases the dynamic strength and brittleness of the fibers in CMCs,leading to more cracks at fracture,which changes from the original bending fracture to shear fracture.In addition,ultrasonic vibration can effectively inhibit the impact of scratching depth and anisotropy on the removal mechanism of CMCs,resulting in a more uniform surface of CMCs after processing.展开更多
Complex genetic architecture is the major cause of heterogeneity in epilepsy,which poses challenges for accurate diagnosis and precise treatment.A large number of epilepsy candidate genes have been identified from cli...Complex genetic architecture is the major cause of heterogeneity in epilepsy,which poses challenges for accurate diagnosis and precise treatment.A large number of epilepsy candidate genes have been identified from clinical studies,particularly with the widespread use of next-generation sequencing.Validating these candidate genes is emerging as a valuable yet challenging task.Drosophila serves as an ideal animal model for validating candidate genes associated with neurogenetic disorders such as epilepsy,due to its rapid reproduction rate,powerful genetic tools,and efficient use of ethological and electrophysiological assays.Here,we systematically summarize the advantageous techniques of the Drosophila model used to investigate epilepsy genes,including genetic tools for manipulating target gene expression,ethological assays for seizure-like behaviors,electrophysiological techniques,and functional imaging for recording neural activity.We then introduce several typical strategies for identifying epilepsy genes and provide new insights into gene-gene interactions in epilepsy with polygenic causes.We summarize well-established precision medicine strategies for epilepsy and discuss prospective treatment options,including drug therapy and gene therapy for genetic epilepsy based on the Drosophila model.Finally,we also address genetic counseling and assisted reproductive technology as potential approaches for the prevention of genetic epilepsy.展开更多
针对洁净板生产线传统调试过程中存在的周期长、成本高、可靠性低等问题,提出了一种基于数字孪生理念的虚拟调试系统架构。通过使用Visual Components构建高保真三维仿真模型,结合Siemens TIA Portal开发模块化控制逻辑,并利用KEP Serve...针对洁净板生产线传统调试过程中存在的周期长、成本高、可靠性低等问题,提出了一种基于数字孪生理念的虚拟调试系统架构。通过使用Visual Components构建高保真三维仿真模型,结合Siemens TIA Portal开发模块化控制逻辑,并利用KEP Server EX 6搭建OPC UA与TCP/IP混合通信网络,实现了物理仿真层、逻辑控制层和数据交互层的协同集成。以岩棉填充工位为例,进行了端到端信号链路验证与全流程物料流仿真。通过对比仿真与实物机器人的运动数据,验证了该系统在关节角度和角速度方面的平均误差小于0.5°,且误差分布在±0.5°范围内的概率达到97.3%。结果表明,所构建的虚拟调试系统具有较高的运动学精度和可靠性,可为洁净板生产线提供低成本、高效率的数字化预验证平台。展开更多
The aim of the present work is to realize an identification algorithm especially devoted to UAS （unmanned aerial systems）. Because UAS employ low cost sensor, very high measurement noise has to be taken into account...The aim of the present work is to realize an identification algorithm especially devoted to UAS （unmanned aerial systems）. Because UAS employ low cost sensor, very high measurement noise has to be taken into account. Therefore, due to both modelling errors and atmospheric turbulence, noticeable system noise has also to be considered. To cope with both the measurement and system noise, the identification problem addressed in this work is solved by using the FEM （filter error method） approach. A nonlinear mathematical model of the subject aircraft longitudinal dynamics has been tuned up through semi-empirical methods, numerical simulations and ground tests. To take into account model nonlinearities, an EKF （extended Kalman filter） has been implemented to propagate the state. A procedure has been tuned up to determine either aircraft parameters or the process noise. It is noticeable that, because the system noise is treated as unknown parameter, it is possible to identify system affected by noticeable modelling errors. Therefore, the obtained values of process noise covariance matrix can be used to highlight system failure. The obtained results show that the algorithm requires a short computation time to determine aircraft parameter with noticeable precision by using low computation power. The present procedure could be employed to determine the system noise for various mechanical systems, since it is particularly devoted to systems which present dynamics that are difficult to model. Finally, the tuned up off-line EKF should be employed to on-line estimation of either state or unmeasurable inputs like atmospheric turbulence.展开更多
【目的】利用P2C可以定向进入卵巢以及Gal4蛋白可与UAS序列稳定结合的特点,在中华按蚊Anopheles sinensis中建立高效的非胚胎期外源DNA投递技术系统。【方法】注射P2C-Gal4-DsRed重组蛋白至吸血后20 h时的中华按蚊雌成蚊腹部,通过冰冻...【目的】利用P2C可以定向进入卵巢以及Gal4蛋白可与UAS序列稳定结合的特点,在中华按蚊Anopheles sinensis中建立高效的非胚胎期外源DNA投递技术系统。【方法】注射P2C-Gal4-DsRed重组蛋白至吸血后20 h时的中华按蚊雌成蚊腹部,通过冰冻切片荧光观察和Western blot检测分析重组蛋白P2C-Gal4-DsRed在卵巢中的投递效率;制备P2C-Gal4 DNA BINDING重组蛋白,构建包含12×UAS重复基序的转基因质粒和辅助质粒,通过电泳迁移实验分析重组蛋白P2C-Gal4 DNA BINDING和12×UAS重复基序间的体外结合;分别将体外孵育的P2C-Gal4 DNA BINDING+辅助质粒ITF36-12×UAS和P2C-Gal4 DNA BINDING+转基因质粒ITF2-12×UAS afm复合物注射入吸血后20 h时的中华按蚊雌成蚊腹部,于血餐后40 h时提取其卵巢组织DNA,并通过特异性引物PCR扩增和测序分析外源DNA在活体中的投递情况。【结果】100%注射P2C-Gal4-DsRed的中华按蚊雌成蚊卵巢在绿色滤光片下呈现明显的红色荧光,表明P2C-Gal4-DsRed重组蛋白能够被高效地导入雌成蚊卵巢中;P2C-Gal4 DNA BINDING重组蛋白能够与12×UAS重复基序以及含有该重复基序片段的质粒稳定结合;分别有91%和93%的注射了P2C-Gal4 DNA BINDING+ITF36-12×UAS和P2C-Gal4 DNA BINDING+ITF2-12×UAS afm的雌成蚊卵巢组织中能够检测到外源DNA片段。【结论】在中华按蚊中成功建立了基于P2C卵巢导向肽和Gal4-12×UAS重复基序结合特性的外源DNA投递技术体系;通过此技术平台能够便捷、快速和高效地实现质粒等DNA分子在中华按蚊卵巢中的投递,这为进一步简化转基因、过表达及基因敲入等遗传操作奠定了基础。展开更多
Fuel cell is a device that converts the chemical energy in the reactants into the electrical energy after steps of sequential electrochemical processes with no significant impact on the environment. For high altitude ...Fuel cell is a device that converts the chemical energy in the reactants into the electrical energy after steps of sequential electrochemical processes with no significant impact on the environment. For high altitude long endurance (HALE) of unmanned aircraft system (UAS) where fuel cell operates as a prime source of power, the operation and performance of a PEM fuel cell at different level of altitudes is vitally important. In this paper, the impact of direct using extracted air from high altitudes atmosphere in order to feed the stack is investigated, and the governing equations of the supplied air and oxygen to the PEM fuel cell stack are developed. The impact of high altitudes upon the operation and the consumption of air are determined in order to maintain certain level of delivered power to the load. Also the implications associated with operating the PEM fuel cell stack at high altitudes and different technical solutions are proposed. Various modes of Integral, Proportional-Integral, and Proportional-Integral-Derivative controller are introduced and examined for different time setting responses in order to determine the most adequate trade-off choice between fast response and reactants consumption which provides the necessary optimization of the air consumption for the developed model of PEM fuel cell used for UAS operation.展开更多
基金supported by the National Science Foundation for Distinguished Young Scholars of China(No.52325506)the Fundamental Research Funds for the Central Universities(No.DUT22LAB501)。
文摘Ultrasonic-Assisted Grinding(UAG)is a novel manufacturing technology that shows promising promise for use in processing Ceramic Matrix Composites(CMCs).Nevertheless,analyzing the material removal process of CMCs with multidirectional structure during UAG is challenging,impeding the progress and improvement of the UAG process.This work examined the impact of ultrasonic vibration on the dynamic mechanical characteristics during processing.Additionally,we experimentally elucidated the material removal mechanism of CMCs during the scratching process under the influence of vertical vibration.The results indicate that the introduction of ultrasonic vibration causes a strain rate effect,resulting in a modification of the material removal mechanism,subsequently impacting the processing quality.Ultrasonic vibration increases the dynamic strength and brittleness of the fibers in CMCs,leading to more cracks at fracture,which changes from the original bending fracture to shear fracture.In addition,ultrasonic vibration can effectively inhibit the impact of scratching depth and anisotropy on the removal mechanism of CMCs,resulting in a more uniform surface of CMCs after processing.
基金supported by the Guangdong Basic and Applied Basic Research Foundation,No.2022A1515111123(to JQ)。
文摘Complex genetic architecture is the major cause of heterogeneity in epilepsy,which poses challenges for accurate diagnosis and precise treatment.A large number of epilepsy candidate genes have been identified from clinical studies,particularly with the widespread use of next-generation sequencing.Validating these candidate genes is emerging as a valuable yet challenging task.Drosophila serves as an ideal animal model for validating candidate genes associated with neurogenetic disorders such as epilepsy,due to its rapid reproduction rate,powerful genetic tools,and efficient use of ethological and electrophysiological assays.Here,we systematically summarize the advantageous techniques of the Drosophila model used to investigate epilepsy genes,including genetic tools for manipulating target gene expression,ethological assays for seizure-like behaviors,electrophysiological techniques,and functional imaging for recording neural activity.We then introduce several typical strategies for identifying epilepsy genes and provide new insights into gene-gene interactions in epilepsy with polygenic causes.We summarize well-established precision medicine strategies for epilepsy and discuss prospective treatment options,including drug therapy and gene therapy for genetic epilepsy based on the Drosophila model.Finally,we also address genetic counseling and assisted reproductive technology as potential approaches for the prevention of genetic epilepsy.
文摘针对洁净板生产线传统调试过程中存在的周期长、成本高、可靠性低等问题,提出了一种基于数字孪生理念的虚拟调试系统架构。通过使用Visual Components构建高保真三维仿真模型,结合Siemens TIA Portal开发模块化控制逻辑,并利用KEP Server EX 6搭建OPC UA与TCP/IP混合通信网络,实现了物理仿真层、逻辑控制层和数据交互层的协同集成。以岩棉填充工位为例,进行了端到端信号链路验证与全流程物料流仿真。通过对比仿真与实物机器人的运动数据,验证了该系统在关节角度和角速度方面的平均误差小于0.5°,且误差分布在±0.5°范围内的概率达到97.3%。结果表明,所构建的虚拟调试系统具有较高的运动学精度和可靠性,可为洁净板生产线提供低成本、高效率的数字化预验证平台。
文摘The aim of the present work is to realize an identification algorithm especially devoted to UAS （unmanned aerial systems）. Because UAS employ low cost sensor, very high measurement noise has to be taken into account. Therefore, due to both modelling errors and atmospheric turbulence, noticeable system noise has also to be considered. To cope with both the measurement and system noise, the identification problem addressed in this work is solved by using the FEM （filter error method） approach. A nonlinear mathematical model of the subject aircraft longitudinal dynamics has been tuned up through semi-empirical methods, numerical simulations and ground tests. To take into account model nonlinearities, an EKF （extended Kalman filter） has been implemented to propagate the state. A procedure has been tuned up to determine either aircraft parameters or the process noise. It is noticeable that, because the system noise is treated as unknown parameter, it is possible to identify system affected by noticeable modelling errors. Therefore, the obtained values of process noise covariance matrix can be used to highlight system failure. The obtained results show that the algorithm requires a short computation time to determine aircraft parameter with noticeable precision by using low computation power. The present procedure could be employed to determine the system noise for various mechanical systems, since it is particularly devoted to systems which present dynamics that are difficult to model. Finally, the tuned up off-line EKF should be employed to on-line estimation of either state or unmeasurable inputs like atmospheric turbulence.
文摘【目的】利用P2C可以定向进入卵巢以及Gal4蛋白可与UAS序列稳定结合的特点,在中华按蚊Anopheles sinensis中建立高效的非胚胎期外源DNA投递技术系统。【方法】注射P2C-Gal4-DsRed重组蛋白至吸血后20 h时的中华按蚊雌成蚊腹部,通过冰冻切片荧光观察和Western blot检测分析重组蛋白P2C-Gal4-DsRed在卵巢中的投递效率;制备P2C-Gal4 DNA BINDING重组蛋白,构建包含12×UAS重复基序的转基因质粒和辅助质粒,通过电泳迁移实验分析重组蛋白P2C-Gal4 DNA BINDING和12×UAS重复基序间的体外结合;分别将体外孵育的P2C-Gal4 DNA BINDING+辅助质粒ITF36-12×UAS和P2C-Gal4 DNA BINDING+转基因质粒ITF2-12×UAS afm复合物注射入吸血后20 h时的中华按蚊雌成蚊腹部,于血餐后40 h时提取其卵巢组织DNA,并通过特异性引物PCR扩增和测序分析外源DNA在活体中的投递情况。【结果】100%注射P2C-Gal4-DsRed的中华按蚊雌成蚊卵巢在绿色滤光片下呈现明显的红色荧光,表明P2C-Gal4-DsRed重组蛋白能够被高效地导入雌成蚊卵巢中;P2C-Gal4 DNA BINDING重组蛋白能够与12×UAS重复基序以及含有该重复基序片段的质粒稳定结合;分别有91%和93%的注射了P2C-Gal4 DNA BINDING+ITF36-12×UAS和P2C-Gal4 DNA BINDING+ITF2-12×UAS afm的雌成蚊卵巢组织中能够检测到外源DNA片段。【结论】在中华按蚊中成功建立了基于P2C卵巢导向肽和Gal4-12×UAS重复基序结合特性的外源DNA投递技术体系;通过此技术平台能够便捷、快速和高效地实现质粒等DNA分子在中华按蚊卵巢中的投递,这为进一步简化转基因、过表达及基因敲入等遗传操作奠定了基础。
文摘Fuel cell is a device that converts the chemical energy in the reactants into the electrical energy after steps of sequential electrochemical processes with no significant impact on the environment. For high altitude long endurance (HALE) of unmanned aircraft system (UAS) where fuel cell operates as a prime source of power, the operation and performance of a PEM fuel cell at different level of altitudes is vitally important. In this paper, the impact of direct using extracted air from high altitudes atmosphere in order to feed the stack is investigated, and the governing equations of the supplied air and oxygen to the PEM fuel cell stack are developed. The impact of high altitudes upon the operation and the consumption of air are determined in order to maintain certain level of delivered power to the load. Also the implications associated with operating the PEM fuel cell stack at high altitudes and different technical solutions are proposed. Various modes of Integral, Proportional-Integral, and Proportional-Integral-Derivative controller are introduced and examined for different time setting responses in order to determine the most adequate trade-off choice between fast response and reactants consumption which provides the necessary optimization of the air consumption for the developed model of PEM fuel cell used for UAS operation.
