The Bianchi type-IX cosmological model with variable ω has been studied in the scalar tensor theory of gravitation proposed by Saez and Ballester [Phys. Lett. A 113: 467, 1985] in the presence and absence of magnetic...The Bianchi type-IX cosmological model with variable ω has been studied in the scalar tensor theory of gravitation proposed by Saez and Ballester [Phys. Lett. A 113: 467, 1985] in the presence and absence of magnetic field of energy densityρb. A special law of variation of Hubble’s parameter proposed by Berman [Nuovo Cimento 74 B, 182, 1983] has been used to solve the field equations. The physical and kinematical properties of the model are also discussed.展开更多
Bianchi type-IX cosmological models with variable equation of state (EoS) parameter have been investigated in general relativity when universe is filled with dark energy. The field equations have been solved by consid...Bianchi type-IX cosmological models with variable equation of state (EoS) parameter have been investigated in general relativity when universe is filled with dark energy. The field equations have been solved by considering (i) q=B (variable);(ii) , where k and m are constants;(iii) , where k is constant and R is average scale factor;(iv) which gives . This renders early decelerating and late time accelerating cosmological models. The physical and geometrical properties of the models are also discussed.展开更多
目的:探讨微环境中Ⅸ型胶原α1(collagen type IX alpha 1 chain,COL9A1)在结直肠腺癌中的表达及其与肿瘤进展的相关性和临床意义。方法:收集2012年1月至2021年1月手术切除的结直肠癌标本408例,采用免疫组织化学检测结直肠腺癌肿瘤组织...目的:探讨微环境中Ⅸ型胶原α1(collagen type IX alpha 1 chain,COL9A1)在结直肠腺癌中的表达及其与肿瘤进展的相关性和临床意义。方法:收集2012年1月至2021年1月手术切除的结直肠癌标本408例,采用免疫组织化学检测结直肠腺癌肿瘤组织及癌旁正常组织中COL9A1表达,同时检测肿瘤组织中肿瘤蛋白53(tumor protein 53,P53)和错配修复(mismatch repair,MMR)蛋白MLH1、MSH6和PMS2的表达,统计分析COL9A1的表达与各临床病理特征参数的关系,以及与P53突变和MMR状态的相关性,并分析COL9A1阳性表达患者的预后情况。结果:COL9A1在结直肠腺癌肿瘤组织中表达显著低于癌旁正常组织(P<0.001);COL9A1的表达与肿瘤浸润深度、临床分期和肠系膜淋巴结转移有关(χ^(2)=16.943、89.031和84.814;均P<0.001),而与P53突变和MMR状态无关(χ^(2)=0.677、1.260,均P>0.05);Log-rank检验显示COL9A1阴性表达患者的无进展生存期(progression free survival,PFS)和总体生存期(overall survival,OS)显著低于COL9A1阳性表达患者(分别P<0.001,P=0.040)。结论:结直肠腺癌中COL9A1蛋白的表达缺失与肿瘤浸润及转移密切相关,并提示不良预后,这可为结直肠癌预后评估、药物筛选等提供可能的分子标志物和治疗策略。展开更多
为探究鸭疫里默氏杆菌(RA)IX型分泌系统(T9SS)分泌的假定蛋白AS87_05150的功能,本研究采用自杀质粒同源重组的方法构建RA Yb2株AS87_05150基因缺失株Yb2Δ5150及其回补株cYb2Δ5150,经PCR及测序鉴定,结果显示AS87_05150基因缺失株Yb2Δ5...为探究鸭疫里默氏杆菌(RA)IX型分泌系统(T9SS)分泌的假定蛋白AS87_05150的功能,本研究采用自杀质粒同源重组的方法构建RA Yb2株AS87_05150基因缺失株Yb2Δ5150及其回补株cYb2Δ5150,经PCR及测序鉴定,结果显示AS87_05150基因缺失株Yb2Δ5150和回补株cYb2Δ5150均正确构建,缺失株的表型为16S r RNA^(+)AS87_05150 ORF^(-),回补株的表型为16S r RNA^(+)AS87_05150 ORF^(+)。采用荧光定量PCR(qPCR)检测Yb2、Yb2Δ5150和cYb2Δ5150株AS87_05150基因的转录水平;根据上述3株菌不同时间的OD_(600nm)值绘制各菌株的生长曲线;采用微量结晶紫法检测各菌株生物被膜(BF)的形成能力;将10^(7)cfu/孔的Yb2、Yb2Δ5150和cYb2Δ5150株分别感染Vero细胞,检测各菌株对Vero细胞的黏附和侵袭力。q PCR检测结果显示,cYb2Δ5150株中AS87_05150 m RNA相对转录水平比Yb2株高约19倍(P<0.0001),Yb2Δ5150株未扩增到AS87_05150基因;生长曲线结果显示,Yb2Δ5150与Yb2株的生长曲线基本一致,但cYb2Δ5150生长速度与Yb2Δ5150株相比极显著下降(P<0.01);BF测定结果显示,Yb2Δ5150和cYb2Δ5150株BF的形成能力与Yb2株相比均极显著降低(P<0.0001);黏附和侵袭力结果显示,这3株菌对Vero细胞的黏附和侵袭力均无显著差异。将3株菌分别以10^(8)cfu/只~10^(4)cfu/只、10^(10)cfu/只~10^(6)cfu/只和10^(9)cfu/只~10^(5)cfu/只的剂量经肌肉注射接种雏鸭,感染后观察雏鸭的发病情况,并采用Reed-Muench法计算3株菌对雏鸭的半数致死量(LD_(50));将3株菌均以10^(7)cfu/只经肌肉注射感染雏鸭,24 h后剖杀,无菌采集各组鸭血液、肝脏和脑组织,作相应处理后涂板采用平板计数法测定载菌量。根据各组鸭的死亡情况,计算Yb2、Yb2Δ5150和cYb2Δ5150株的LD_(50)分别为6.85×10^(5)cfu、8.75×10^(7)cfu和4.681×10^(8)cfu,即缺失株LD_(50)比野生株高约127倍,但回补株的LD_(50)比缺失株高约5倍。载菌量测定结果显示,Yb2Δ5150株和cYb2Δ5150株感染鸭血液、肝脏、脑组织中的细菌载量比Yb2株感染鸭的载菌量均极显著降低(P<0.001),但前两者之间上述指标均无显著差异。本实验结果表明AS87_05150与RA Yb2株的BF形成和致病性相关,但回补株对雏鸭的毒力和致病性均未回复到野生株的水平。本研究为进一步解析RA AS87_05150的功能及分子致病机制等奠定了基础。展开更多
IX型分泌系统(Type IX Secretion System,T9SS)是一种最新发现的存在于许多革兰氏阴性细菌中的分泌系统。T9SS参与细菌的毒力和滑行运动及复杂生物聚合物的降解过程。近年来,与T9SS相关的研究一直都是微生物学领域关注的热点。本文就T9S...IX型分泌系统(Type IX Secretion System,T9SS)是一种最新发现的存在于许多革兰氏阴性细菌中的分泌系统。T9SS参与细菌的毒力和滑行运动及复杂生物聚合物的降解过程。近年来,与T9SS相关的研究一直都是微生物学领域关注的热点。本文就T9SS的发现、组成与结构、分泌机制及调控机制等方面的研究进展进行综述,以期为进一步解析细菌的T9SS提供新的思路。展开更多
Previous studies have demonstrated that a missense single-nucleotide polymorphism variant （2316A〉G;rs2230739）of the adenylate cyclase type IX gene was associated with bipolar disorder and affective disorder.We dete...Previous studies have demonstrated that a missense single-nucleotide polymorphism variant （2316A〉G;rs2230739）of the adenylate cyclase type IX gene was associated with bipolar disorder and affective disorder.We determined genotype and allele frequencies using a ligase detection reaction method in 315 patients with major depressive disorder and 278 unrelated, sex-matched healthy control subjects.We did not detect any statistically significant differences in genotype and allele frequencies between patients and healthy control subjects.Furthermore,we found no significant difference between genders in major depressive disorder,nor between patients and controls in the same gender.These results suggest that 2316A〉G（rs2230739）may not be a risk factor for increasing susceptibility to major depressive disorder in the Chinese Han population.展开更多
Field equations in the presence of perfect fluid distribution are obtained in a scalar tensor theory of gravitation proposed by Brans and Dicke[1] with the aid of Bianchi type-II, VIII & IX metrics. Exact prefect ...Field equations in the presence of perfect fluid distribution are obtained in a scalar tensor theory of gravitation proposed by Brans and Dicke[1] with the aid of Bianchi type-II, VIII & IX metrics. Exact prefect fluid Bianchi type- IX cosmological model is presented since other models doesn’t exist in Brans-Dicke scalar tensor theory of gravitation. Some physical properties of the model are also discussed.展开更多
文摘The Bianchi type-IX cosmological model with variable ω has been studied in the scalar tensor theory of gravitation proposed by Saez and Ballester [Phys. Lett. A 113: 467, 1985] in the presence and absence of magnetic field of energy densityρb. A special law of variation of Hubble’s parameter proposed by Berman [Nuovo Cimento 74 B, 182, 1983] has been used to solve the field equations. The physical and kinematical properties of the model are also discussed.
文摘Bianchi type-IX cosmological models with variable equation of state (EoS) parameter have been investigated in general relativity when universe is filled with dark energy. The field equations have been solved by considering (i) q=B (variable);(ii) , where k and m are constants;(iii) , where k is constant and R is average scale factor;(iv) which gives . This renders early decelerating and late time accelerating cosmological models. The physical and geometrical properties of the models are also discussed.
文摘目的:探讨微环境中Ⅸ型胶原α1(collagen type IX alpha 1 chain,COL9A1)在结直肠腺癌中的表达及其与肿瘤进展的相关性和临床意义。方法:收集2012年1月至2021年1月手术切除的结直肠癌标本408例,采用免疫组织化学检测结直肠腺癌肿瘤组织及癌旁正常组织中COL9A1表达,同时检测肿瘤组织中肿瘤蛋白53(tumor protein 53,P53)和错配修复(mismatch repair,MMR)蛋白MLH1、MSH6和PMS2的表达,统计分析COL9A1的表达与各临床病理特征参数的关系,以及与P53突变和MMR状态的相关性,并分析COL9A1阳性表达患者的预后情况。结果:COL9A1在结直肠腺癌肿瘤组织中表达显著低于癌旁正常组织(P<0.001);COL9A1的表达与肿瘤浸润深度、临床分期和肠系膜淋巴结转移有关(χ^(2)=16.943、89.031和84.814;均P<0.001),而与P53突变和MMR状态无关(χ^(2)=0.677、1.260,均P>0.05);Log-rank检验显示COL9A1阴性表达患者的无进展生存期(progression free survival,PFS)和总体生存期(overall survival,OS)显著低于COL9A1阳性表达患者(分别P<0.001,P=0.040)。结论:结直肠腺癌中COL9A1蛋白的表达缺失与肿瘤浸润及转移密切相关,并提示不良预后,这可为结直肠癌预后评估、药物筛选等提供可能的分子标志物和治疗策略。
文摘为探究鸭疫里默氏杆菌(RA)IX型分泌系统(T9SS)分泌的假定蛋白AS87_05150的功能,本研究采用自杀质粒同源重组的方法构建RA Yb2株AS87_05150基因缺失株Yb2Δ5150及其回补株cYb2Δ5150,经PCR及测序鉴定,结果显示AS87_05150基因缺失株Yb2Δ5150和回补株cYb2Δ5150均正确构建,缺失株的表型为16S r RNA^(+)AS87_05150 ORF^(-),回补株的表型为16S r RNA^(+)AS87_05150 ORF^(+)。采用荧光定量PCR(qPCR)检测Yb2、Yb2Δ5150和cYb2Δ5150株AS87_05150基因的转录水平;根据上述3株菌不同时间的OD_(600nm)值绘制各菌株的生长曲线;采用微量结晶紫法检测各菌株生物被膜(BF)的形成能力;将10^(7)cfu/孔的Yb2、Yb2Δ5150和cYb2Δ5150株分别感染Vero细胞,检测各菌株对Vero细胞的黏附和侵袭力。q PCR检测结果显示,cYb2Δ5150株中AS87_05150 m RNA相对转录水平比Yb2株高约19倍(P<0.0001),Yb2Δ5150株未扩增到AS87_05150基因;生长曲线结果显示,Yb2Δ5150与Yb2株的生长曲线基本一致,但cYb2Δ5150生长速度与Yb2Δ5150株相比极显著下降(P<0.01);BF测定结果显示,Yb2Δ5150和cYb2Δ5150株BF的形成能力与Yb2株相比均极显著降低(P<0.0001);黏附和侵袭力结果显示,这3株菌对Vero细胞的黏附和侵袭力均无显著差异。将3株菌分别以10^(8)cfu/只~10^(4)cfu/只、10^(10)cfu/只~10^(6)cfu/只和10^(9)cfu/只~10^(5)cfu/只的剂量经肌肉注射接种雏鸭,感染后观察雏鸭的发病情况,并采用Reed-Muench法计算3株菌对雏鸭的半数致死量(LD_(50));将3株菌均以10^(7)cfu/只经肌肉注射感染雏鸭,24 h后剖杀,无菌采集各组鸭血液、肝脏和脑组织,作相应处理后涂板采用平板计数法测定载菌量。根据各组鸭的死亡情况,计算Yb2、Yb2Δ5150和cYb2Δ5150株的LD_(50)分别为6.85×10^(5)cfu、8.75×10^(7)cfu和4.681×10^(8)cfu,即缺失株LD_(50)比野生株高约127倍,但回补株的LD_(50)比缺失株高约5倍。载菌量测定结果显示,Yb2Δ5150株和cYb2Δ5150株感染鸭血液、肝脏、脑组织中的细菌载量比Yb2株感染鸭的载菌量均极显著降低(P<0.001),但前两者之间上述指标均无显著差异。本实验结果表明AS87_05150与RA Yb2株的BF形成和致病性相关,但回补株对雏鸭的毒力和致病性均未回复到野生株的水平。本研究为进一步解析RA AS87_05150的功能及分子致病机制等奠定了基础。
文摘IX型分泌系统(Type IX Secretion System,T9SS)是一种最新发现的存在于许多革兰氏阴性细菌中的分泌系统。T9SS参与细菌的毒力和滑行运动及复杂生物聚合物的降解过程。近年来,与T9SS相关的研究一直都是微生物学领域关注的热点。本文就T9SS的发现、组成与结构、分泌机制及调控机制等方面的研究进展进行综述,以期为进一步解析细菌的T9SS提供新的思路。
基金funded by Natural Science Foundation of Department of Education of Henan Province,No.2009B20005
文摘Previous studies have demonstrated that a missense single-nucleotide polymorphism variant （2316A〉G;rs2230739）of the adenylate cyclase type IX gene was associated with bipolar disorder and affective disorder.We determined genotype and allele frequencies using a ligase detection reaction method in 315 patients with major depressive disorder and 278 unrelated, sex-matched healthy control subjects.We did not detect any statistically significant differences in genotype and allele frequencies between patients and healthy control subjects.Furthermore,we found no significant difference between genders in major depressive disorder,nor between patients and controls in the same gender.These results suggest that 2316A〉G（rs2230739）may not be a risk factor for increasing susceptibility to major depressive disorder in the Chinese Han population.
文摘Field equations in the presence of perfect fluid distribution are obtained in a scalar tensor theory of gravitation proposed by Brans and Dicke[1] with the aid of Bianchi type-II, VIII & IX metrics. Exact prefect fluid Bianchi type- IX cosmological model is presented since other models doesn’t exist in Brans-Dicke scalar tensor theory of gravitation. Some physical properties of the model are also discussed.
