The adsorption and separation of antibody drugs are of great significance,but the promising hydrophobic charge induction chromatography(HCIC)and boronate affinity chromatography(BAC)suffer from low specific due to the...The adsorption and separation of antibody drugs are of great significance,but the promising hydrophobic charge induction chromatography(HCIC)and boronate affinity chromatography(BAC)suffer from low specific due to the limitations of single-site adsorption mechanism as well as low adsorption capacity of adsorbents,resulting in a lower purity and recovery of antibodies.To address this issue,this work proposes a two-site synergistic binding strategy integrating HCIC and BAC mechanism on a polymer brushes-grafted adsorbent.Five adsorbents were easily created by polymerizing the mixed monomers of 5-acryloylaminobenzimidazole,3-acryloylamide phenylboronic acid and acrylamide on surface of agarose gel via activators regenerated by electron transfer for atom transfer radical polymerization(ARGET ATRP).The molecular docking implies that the two-site synergistic binding towards immunoglobulin G(IgG)originates from the closely adjacent boronic and benzimidazole side groups in the polymer chains with monomer ratio of 1:1:0.The inference was verified by the effect of three monomer ratios and adsorption conditions on the adsorption performance of IgG.The adsorbent with two-site synergy possesses an excellent specific,enhanced affinity(K_(d)=3.9×10^(-6)mol/L)and adsorption capacity(Q_(m)=253 mg/g)towards IgG.Benefiting from the advantages,IgG from serum and monoclonal antibody(m Ab)from cell culture achieve purities of 95.8%and 98.3%,and recoveries of 95.7%and 97.5%,respectively.The results are comparable to those with protein A adsorbent considered to have the best specific so far,indicating the potential of the two-site synergistic binding strategy in the purification of antibody drugs.展开更多
In this work, we propose an algebraic recursion method to study the dynamical evolution of the two-site Bose- Hubbard model. We analyze its properties from the viewpoints of single partite purity, energy, and trace di...In this work, we propose an algebraic recursion method to study the dynamical evolution of the two-site Bose- Hubbard model. We analyze its properties from the viewpoints of single partite purity, energy, and trace distance, in which the model is considered as a typical bipartite system. The analytical expressions for the quantities are derived. We show that the purity can well reflect the transition between different regimes for the system. In addition, we demonstrate that the transition from the delocalization regime to the self-trapping regime with the ratio r/increasing not only happens for an initially local state but also for any initial states. Furthermore, we confirm that the dynamics of the system presents a periodicity for η = 0 and the period is tc =π/2J when the initial state is symmetric.展开更多
Background Phacotrabeculectomy can be performed using one-site or two-site incisions.This meta-analysis evaluated the efficacy and tolerability of one-site versus two-site phacotrabecuiectomy in the treatment of patie...Background Phacotrabeculectomy can be performed using one-site or two-site incisions.This meta-analysis evaluated the efficacy and tolerability of one-site versus two-site phacotrabecuiectomy in the treatment of patients with coexisting cataract and glaucoma.Methods A comprehensive literature search was performed according to the Cochrane Collaboration methodology toidentify randomized controlled clinical trials comparing one-site with two-site phacotrabeculectomy.Studies meeting our predefined criteria were included in the meta-analysis.Efficacy estimates were measured by weighted mean difference （WMD） for the percentage intraocular pressure （IOP） reduction from baseline to end point, relative risk （RR） for the proportion of patients with a best-corrected visual acuity （BCVA） of 0.5 or better after surgery and complete success rates.Tolerability estimates were measured by RR for adverse events.All of outcomes were reported with 95% confidence interval （95% CI）.Data were synthesised by Stata 10.1 for Windows.Results Two-site phacotrabeculectomy was associated with greater reductions in IOP than the one-site procedure （WMD： -5.99, 95% CI： -10.74-1.24, P=0.01）.A greater proportion of patients also achieved a BCVA of 0.5 or better （RR：0.91, 95% CI： 0.74-1.12, P=0.36） and the target IOP without anti-glaucoma medication at the study end point （RR： 0.94,95% CI： 0.83-1.07, P=0.34） after two-site than one-site phacotrabeculectomy, but the differences were not significant.There were no significant differences in adverse events between two surgical procedures.Conclusions Two-site phacotrabeculectomy is superior to one-site phacotrabeculectomy in reducing IOP, but other post-operative effects are similar.One-site and two-site phacotrabeculectomies have similar adverse event rates.展开更多
目的探讨肥胖患者施行经脐单孔腹腔镜手术(transumbilical laparoendoscopic single site surgery,TU-LESS)加一个操作孔的两孔腹腔镜子宫肌瘤剔除术的安全性。方法回顾性分析2020年7月~2023年7月139例两孔腹腔镜子宫肌瘤剔除术资料,其...目的探讨肥胖患者施行经脐单孔腹腔镜手术(transumbilical laparoendoscopic single site surgery,TU-LESS)加一个操作孔的两孔腹腔镜子宫肌瘤剔除术的安全性。方法回顾性分析2020年7月~2023年7月139例两孔腹腔镜子宫肌瘤剔除术资料,其中24例体重指数(BMI)≥28,115例BMI<28。TU-LESS入路基础上在左下腹增加一个5 mm辅助操作孔,使用传统腹腔镜及手术器械实施手术。比较2组围术期指标。结果1例(BMI 31.6)因手术操作困难增加第2个辅助孔,其余138例均未中转手术方式,术中、术后未出现严重并发症。2组手术时间、术中出血量、手术前后血红蛋白差值、术后首次排气时间、术后24 h疼痛视觉模拟评分(Visual Analogue Scale,VAS)、术后发热、术后住院时间、住院费用差异均无显著性(P>0.05)。结论肥胖患者行TU-LESS加一个操作孔的两孔腹腔镜子宫肌瘤剔除术安全、可行。展开更多
基金supported by the National Natural Science Foundation of China(Nos.22274129,21974106 and 22074117)。
文摘The adsorption and separation of antibody drugs are of great significance,but the promising hydrophobic charge induction chromatography(HCIC)and boronate affinity chromatography(BAC)suffer from low specific due to the limitations of single-site adsorption mechanism as well as low adsorption capacity of adsorbents,resulting in a lower purity and recovery of antibodies.To address this issue,this work proposes a two-site synergistic binding strategy integrating HCIC and BAC mechanism on a polymer brushes-grafted adsorbent.Five adsorbents were easily created by polymerizing the mixed monomers of 5-acryloylaminobenzimidazole,3-acryloylamide phenylboronic acid and acrylamide on surface of agarose gel via activators regenerated by electron transfer for atom transfer radical polymerization(ARGET ATRP).The molecular docking implies that the two-site synergistic binding towards immunoglobulin G(IgG)originates from the closely adjacent boronic and benzimidazole side groups in the polymer chains with monomer ratio of 1:1:0.The inference was verified by the effect of three monomer ratios and adsorption conditions on the adsorption performance of IgG.The adsorbent with two-site synergy possesses an excellent specific,enhanced affinity(K_(d)=3.9×10^(-6)mol/L)and adsorption capacity(Q_(m)=253 mg/g)towards IgG.Benefiting from the advantages,IgG from serum and monoclonal antibody(m Ab)from cell culture achieve purities of 95.8%and 98.3%,and recoveries of 95.7%and 97.5%,respectively.The results are comparable to those with protein A adsorbent considered to have the best specific so far,indicating the potential of the two-site synergistic binding strategy in the purification of antibody drugs.
基金Project supported by the National Natural Science Foundation of China(Grant Nos.91021009 and 21073110)
文摘In this work, we propose an algebraic recursion method to study the dynamical evolution of the two-site Bose- Hubbard model. We analyze its properties from the viewpoints of single partite purity, energy, and trace distance, in which the model is considered as a typical bipartite system. The analytical expressions for the quantities are derived. We show that the purity can well reflect the transition between different regimes for the system. In addition, we demonstrate that the transition from the delocalization regime to the self-trapping regime with the ratio r/increasing not only happens for an initially local state but also for any initial states. Furthermore, we confirm that the dynamics of the system presents a periodicity for η = 0 and the period is tc =π/2J when the initial state is symmetric.
文摘Background Phacotrabeculectomy can be performed using one-site or two-site incisions.This meta-analysis evaluated the efficacy and tolerability of one-site versus two-site phacotrabecuiectomy in the treatment of patients with coexisting cataract and glaucoma.Methods A comprehensive literature search was performed according to the Cochrane Collaboration methodology toidentify randomized controlled clinical trials comparing one-site with two-site phacotrabeculectomy.Studies meeting our predefined criteria were included in the meta-analysis.Efficacy estimates were measured by weighted mean difference （WMD） for the percentage intraocular pressure （IOP） reduction from baseline to end point, relative risk （RR） for the proportion of patients with a best-corrected visual acuity （BCVA） of 0.5 or better after surgery and complete success rates.Tolerability estimates were measured by RR for adverse events.All of outcomes were reported with 95% confidence interval （95% CI）.Data were synthesised by Stata 10.1 for Windows.Results Two-site phacotrabeculectomy was associated with greater reductions in IOP than the one-site procedure （WMD： -5.99, 95% CI： -10.74-1.24, P=0.01）.A greater proportion of patients also achieved a BCVA of 0.5 or better （RR：0.91, 95% CI： 0.74-1.12, P=0.36） and the target IOP without anti-glaucoma medication at the study end point （RR： 0.94,95% CI： 0.83-1.07, P=0.34） after two-site than one-site phacotrabeculectomy, but the differences were not significant.There were no significant differences in adverse events between two surgical procedures.Conclusions Two-site phacotrabeculectomy is superior to one-site phacotrabeculectomy in reducing IOP, but other post-operative effects are similar.One-site and two-site phacotrabeculectomies have similar adverse event rates.
文摘目的探讨肥胖患者施行经脐单孔腹腔镜手术(transumbilical laparoendoscopic single site surgery,TU-LESS)加一个操作孔的两孔腹腔镜子宫肌瘤剔除术的安全性。方法回顾性分析2020年7月~2023年7月139例两孔腹腔镜子宫肌瘤剔除术资料,其中24例体重指数(BMI)≥28,115例BMI<28。TU-LESS入路基础上在左下腹增加一个5 mm辅助操作孔,使用传统腹腔镜及手术器械实施手术。比较2组围术期指标。结果1例(BMI 31.6)因手术操作困难增加第2个辅助孔,其余138例均未中转手术方式,术中、术后未出现严重并发症。2组手术时间、术中出血量、手术前后血红蛋白差值、术后首次排气时间、术后24 h疼痛视觉模拟评分(Visual Analogue Scale,VAS)、术后发热、术后住院时间、住院费用差异均无显著性(P>0.05)。结论肥胖患者行TU-LESS加一个操作孔的两孔腹腔镜子宫肌瘤剔除术安全、可行。
