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Influence of sampling intensity on performance of two-phase forest inventory using airborne laser scanning
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作者 Marek Lisańczuk Krzysztof Mitelsztedt +4 位作者 Karolina Parkitna Grzegorz Krok Krzysztof Stereńczak Emilia Wysocka-Fijorek Stanisław Miścicki 《Forest Ecosystems》 SCIE CSCD 2020年第4期871-886,共16页
Background: Forest inventories have always been a primary information source concerning the forest ecosystem state. Various applied survey approaches arise from the numerous important factors during sampling scheme pl... Background: Forest inventories have always been a primary information source concerning the forest ecosystem state. Various applied survey approaches arise from the numerous important factors during sampling scheme planning. Paramount aspects include the survey goal and scale, target population inherent variation and patterns,and available resources. The last factor commonly inhibits the goal, and compromises have to be made. Airborne laser scanning(ALS) has been intensively tested as a cost-effective option for forest inventories. Despite existing foundations, research has provided disparate results. Environmental conditions are one of the factors greatly influencing inventory performance. Therefore, a need for site-related sampling optimization is well founded.Moreover, as stands are the basic operational unit of managed forest holdings, few related studies have presented stand-level results. As such, herein, we tested the sampling intensity influence on the performance of the ALSenhanced stand-level inventory.Results: Distributions of possible errors were plotted by comparing ALS model estimates, with reference values derived from field surveys of 3300 sample plots and more than 300 control stands located in 5 forest districts. No improvement in results was observed due to the scanning density. The variance in obtained errors stabilized in the interval of 200–300 sample plots, maintaining the bias within +/-5% and the precision above 80%. The sample plot area affected scores mostly when transitioning from 100 to 200 m2. Only a slight gain was observed when bigger plots were used.Conclusions: ALS-enhanced inventories effectively address the demand for comprehensive and detailed information on the structure of single stands over vast areas. Knowledge of the relation between the sampling intensity and accuracy of ALS estimates allows the determination of certain sampling intensity thresholds. This should be useful when matching the required sample size and accuracy with available resources. Site optimization may be necessary, as certain errors may occur due to the sampling scheme, estimator type or forest site, making these factors worth further consideration. 展开更多
关键词 Forest inventory Sampling intensity Airborne laser scanning Growing stock volume Regression
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Pore-scale gas–water two-phase flow and relative permeability characteristics of disassociated hydrate reservoir 被引量:1
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作者 Yu-Xuan Xia Derek Elsworth +3 位作者 Sai Xu Xuan-Zhe Xia Jian-Chao Cai Cheng Lu 《Petroleum Science》 2025年第8期3344-3356,共13页
Clayey-silt natural gas hydrate reservoirs in the South China Sea exhibit loose and unconsolidated structures, heterogeneous pore structures, high clay mineral contents, and strong hydrophilicity. These characteristic... Clayey-silt natural gas hydrate reservoirs in the South China Sea exhibit loose and unconsolidated structures, heterogeneous pore structures, high clay mineral contents, and strong hydrophilicity. These characteristics complicate the gas-water two-phase flow process in porous media following hydrate decomposition, posing challenges for efficient development. This study examines the transport response of clayey-silt reservoir samples from the Shenhu area using gas-water two-phase flow experiments and CT scanning to explore changes in pore structure, gas-water distribution, and relative permeability under varying flow conditions. The results indicate that pore heterogeneity significantly influences flow characteristics. Gas preferentially displaces water in larger pores, forming fracture-like pores, which serve as preferential flow channels for gas migration. The preferential flow channels enhance gas-phase permeability up to 19 times that of the water phase when fluid pressures exceed total stresses. However,small pores retain liquid, leading to a high residual water saturation of 0.561. CT imaging reveals that these hydro-fractures improve gas permeability but also confine gas flow to specific channels. Pore network analysis shows that gas injection expands the pore-throat network, enhancing connectivity and forming fracture-like pores. Residual water remains trapped in smaller pores and throats, while structural changes, including new fractures, improve gas flow pathways and overall connectivity. Relative permeability curves demonstrate a narrow gas-water cocurrent-flow zone, a right-shifted iso-permeability point and high reservoir capillary pressure, indicating a strong "water-blocking" effect. The findings suggest that optimizing reservoir stimulation techniques to enhance fracture formation, reduce residual water saturation, and improve gas flow capacity is critical for efficient hydrate reservoir development. 展开更多
关键词 Clayey-silt reservoir Gasewater two-phase flow CT scanning Relative permeability Pore network model
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In-situ observation of nonmetallic inclusions in steel using confocal scanning laser microscopy:A review 被引量:2
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作者 Ying Ren Lifeng Zhang 《International Journal of Minerals,Metallurgy and Materials》 2025年第5期975-991,共17页
The characteristics of nonmetallic inclusions formed during steel production have a significant influence on steel performance.In this paper,studies on inclusions using confocal scanning laser microscopy(CSLM)are revi... The characteristics of nonmetallic inclusions formed during steel production have a significant influence on steel performance.In this paper,studies on inclusions using confocal scanning laser microscopy(CSLM)are reviewed and summarized,particularly the col-lision of various inclusions,dissolution of inclusions in liquid slag,and reactions between inclusions and steel.Solid inclusions exhibited a high collision tendency,whereas pure liquid inclusions exhibited minimal collisions because of the small attraction force induced by their<90°contact angle with molten steel.The collision of complex inclusions in molten steel was not included in the scope of this study and should be evaluated in future studies.Higher CaO/Al_(2)O_(3)and CaO/SiO_(2)ratios in liquid slag promoted the dissolution of Al_(2)O_(3)-based in-clusions.The formation of solid phases in the slag should be prevented to improve dissolution of inclusions.To accurately simulate the dissolution of inclusions in liquid slag,in-situ observation of the dissolution of inclusions at the steel-slag interface is necessary.Using a combination of CSLM and scanning electron microscopy-energy dispersive spectroscopy,the composition and morphological evolution of the inclusions during their modification by the dissolved elements in steel were observed and analyzed.Although the in-situ observa-tion of MnS and TiN precipitations has been widely studied,the in-situ observation of the evolution of oxide inclusions in steel during so-lidification and heating processes has rarely been reported.The effects of temperature,heating and cooling rates,and inclusion character-istics on the formation of acicular ferrites(AFs)have been widely studied.At a cooling rate of 3-5 K/s,the order of AF growth rate in-duced by different inclusions,as reported in literature,is Ti-O<Ti-Ca-Zr-Al-O<Mg-O<Ti-Zr-Al-O<Mn-Ti-Al-O<Ti-Al-O<Zr-Ti-Al-O.Further comprehensive experiments are required to investigate the quantitative relationship between the formation of AFs and inclusions. 展开更多
关键词 INCLUSION STEEL in-situ observation confocal scanning laser microscopy
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Sparse pipeline wall information-based data-driven reconstruction for solid–liquid two-phase flow in flexible vibrating pipelines 被引量:1
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作者 Shengpeng Xiao Chuyi Wan +6 位作者 Hongbo Zhu Dai Zhou Juxi Hu Mengmeng Zhang Yuankun Sun Yan Bao Ke Zhao 《International Journal of Mining Science and Technology》 2025年第11期1885-1903,共19页
Deep-sea mineral resource transportation predominantly utilizes hydraulic pipeline methodology.Environmental factors induce vibrations in flexible pipelines,thereby affecting the internal flow characteristics.Therefor... Deep-sea mineral resource transportation predominantly utilizes hydraulic pipeline methodology.Environmental factors induce vibrations in flexible pipelines,thereby affecting the internal flow characteristics.Therefore,real-time monitoring of solid–liquid two-phase flow in pipelines is crucial for system maintenance.This study develops an autoencoder-based deep learning framework to reconstruct three-dimensional solid–liquid two-phase flow within flexible vibrating pipelines utilizing sparse wall information from sensors.Within this framework,separate X-model and F-model with distinct hidden-layer structures are established to reconstruct the coordinates and flow field information on the computational domain grid of the pipeline under traveling wave vibration.Following hyperparameter optimization,the models achieved high reconstruction accuracy,demonstrating R^(2)values of 0.990 and 0.945,respectively.The models’robustness is evaluated across three aspects:vibration parameters,physical fields,and vibration modes,demonstrating good reconstruction performance.Results concerning sensors show that 20 sensors(0.06%of total grids)achieve a balance between accuracy and cost,with superior accuracy obtained when arranged along the full length of the pipe compared to a dense arrangement at the front end.The models exhibited a signal-to-noise ratio tolerance of approximately 27 dB,with reconstruction accuracy being more affected by sensor failures at both ends of the pipeline. 展开更多
关键词 Particles Solid-liquid two-phase flow Vibration Flexible pipelines Deep learning RECONSTRUCTION
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Mechanism of the Fluidelastic Instability of a Flexible Tube with a Squeeze Film Within a Rigid Tube Array Subjected to Two-Phase Flow 被引量:1
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作者 YANG Shi-hao LAI Jiang ZHU Hong-jun 《China Ocean Engineering》 2025年第5期855-865,共11页
The influence of the squeeze film between the tube and the support structure on flow-induced vibrations is a critical factor in tube bundles subjected to two-phase cross-flow.This aspect can significantly alter the th... The influence of the squeeze film between the tube and the support structure on flow-induced vibrations is a critical factor in tube bundles subjected to two-phase cross-flow.This aspect can significantly alter the threshold for fluidelastic instability and affect heat transfer efficiency.This paper presents a mathematical model incorporating the squeeze film force between the tube and the support structure.We aim to clarify the mechanisms underlying fluidelastic instability in tube bundle systems exposed to two-phase flow.Using a self-developed computer program,we performed numerical calculations to examine the influence of the squeeze film on the threshold of fluidelastic instability in the tube bundle system.Furthermore,we analyzed how the thickness and length of the squeeze film affect both the underlying mechanisms and the critical velocity of fluidelastic instability. 展开更多
关键词 fluidelastic instability tube bundles squeeze film eigenvalue problem two-phase flow
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Dynamic Behavior of a Pipe Conveying a Gas-Liquid Two-Phase Flow Under External Excitations 被引量:1
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作者 FU Guang-ming WANG Xiao +4 位作者 JIAO Hui-lin WANG Bo-ying SHAN Zheng-feng SUN Bao-jiang SU Jian 《China Ocean Engineering》 2025年第5期822-838,共17页
This work investigated the dynamic behavior of vertical pipes conveying gas-liquid two-phase flow when subjected to external excitations at both ends.Even with minimal excitation amplitude,resonance can occur when the... This work investigated the dynamic behavior of vertical pipes conveying gas-liquid two-phase flow when subjected to external excitations at both ends.Even with minimal excitation amplitude,resonance can occur when the excitation frequency aligns with the natural frequency of the pipe,significantly increasing the degree of operational risk.The governing equation of motion based on the Euler-Bernoulli beam is derived for the relative deflection with stationary simply supported ends,with the effects of the external excitations represented by source terms distributed along the pipe length.The fourth-order partial differential equation is solved via the generalized integral transform technique(GITT),with the solution successfully verified via comparison with results in the literature.A comprehensive analysis of the vibration phenomena and changes in the motion state of the pipe is conducted for three classes of external excitation conditions:same frequency and amplitude(SFSA),same frequency but different amplitudes(SFDA),and different frequencies and amplitudes(DFDA).The numerical results show that with increasing gas volume fraction,the position corresponding to the maximum vibration displacement shifts upward.Compared with conditions without external excitation,the vibration displacement of the pipe conveying two-phase flow under external excitation increases significantly.The frequency of external excitation has a significant effect on the dynamic behavior of a pipe conveying two-phase flow. 展开更多
关键词 pipe conveying fluid integral transform two-phase flow external excitations dynamic response forced vibrations
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In-situ observation and analysis of high temperature behavior of carbides in GCr15 bearing steel by confocal laser scanning microscopy 被引量:2
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作者 Jun Ren Yue Teng +4 位作者 Xiang Liu Xi Xu Hui-gai Li Ke Han Qi-jie Zhai 《Journal of Iron and Steel Research International》 2025年第2期409-417,共9页
The high-temperature dissolution behavior of primary carbides in samples taken from GCr15 continuous-casting bloom was observed in-situ by confocal laser scanning microscopy.Equations were fitted to the dissolution ki... The high-temperature dissolution behavior of primary carbides in samples taken from GCr15 continuous-casting bloom was observed in-situ by confocal laser scanning microscopy.Equations were fitted to the dissolution kinetics of primary carbides during either heating or soaking.Dissolution of carbides proceeded in three stages(fast→slow→faster)as either temperature or holding time was increased.During the heating process and during the first and third stages of the soaking process,the original size of the carbides determined the steepness of the slope,but during the middle(“slow”)stage of the soaking process,the slope remained zero.The initial size of the carbides varied greatly,but their final dissolution temperature fell within the narrow range of 1210-1235℃,and the holding time remained within 50 min.Fractal analysis was used to study the morphological characteristics of small and medium-sized carbides during the dissolution process.According to changes in the fractal dimension before and after soaking,the carbides tended to evolve towards a more regular morphology. 展开更多
关键词 Bearing steel High-temperature confocal laser scanning microscope In-situ observation Primary carbide Fractal analysis
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A centroid measurement method based on 3D scanning 被引量:1
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作者 HE Xin LI Zhen 《Journal of Measurement Science and Instrumentation》 2025年第2期186-194,共9页
The centroid coordinate serves as a critical control parameter in motion systems,including aircraft,missiles,rockets,and drones,directly influencing their motion dynamics and control performance.Traditional methods fo... The centroid coordinate serves as a critical control parameter in motion systems,including aircraft,missiles,rockets,and drones,directly influencing their motion dynamics and control performance.Traditional methods for centroid measurement often necessitate custom equipment and specialized positioning devices,leading to high costs and limited accuracy.Here,we present a centroid measurement method that integrates 3D scanning technology,enabling accurate measurement of centroid across various types of objects without the need for specialized positioning fixtures.A theoretical framework for centroid measurement was established,which combined the principle of the multi-point weighing method with 3D scanning technology.The measurement accuracy was evaluated using a designed standard component.Experimental results demonstrate that the discrepancies between the theoretical and the measured centroid of a standard component with various materials and complex shapes in the X,Y,and Z directions are 0.003 mm,0.009 mm,and 0.105 mm,respectively,yielding a spatial deviation of 0.106 mm.Qualitative verification was conducted through experimental validation of three distinct types.They confirmed the reliability of the proposed method,which allowed for accurate centroid measurements of various products without requiring positioning fixtures.This advancement significantly broadened the applicability and scope of centroid measurement devices,offering new theoretical insights and methodologies for the measurement of complex parts and systems. 展开更多
关键词 centroid measurement mass characteristic parameter 3D scanning 3D point cloud data no specialized positioning fixtures multi-point weighing method
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Dual-scale insights of two-phase flow in inter-cleats based on microfluidics:Interface jumps and energy dissipation
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作者 Jicheng Zhang Dawei Lv +3 位作者 Jon Jincai Zhang Feng Wang Dawei Yin Haiyang Yu 《International Journal of Mining Science and Technology》 2025年第3期451-465,共15页
Cleat serves as the primary flow pathway for coalbed methane(CBM)and water.However,few studies consider the impact of local contact on two-phase flow within cleats.A visual generalized model of endogenous cleats was c... Cleat serves as the primary flow pathway for coalbed methane(CBM)and water.However,few studies consider the impact of local contact on two-phase flow within cleats.A visual generalized model of endogenous cleats was constructed based on microfluidics.A microscopic and mesoscopic observation technique was proposed to simultaneously capture gas-liquid interface morphology of pores and throat and the two-phase flow characteristics in entire cleat system.The local contact characteristics of cleats reduced absolute permeability,which resulted in a sharp increase in the starting pressure.The reduced gas flow capacity narrowed the co-infiltration area and decreased water saturation at the isotonic point in a hydrophilic environment.The increased local contact area of cleats weakened gas phase flow capacity and narrowed the co-infiltration area.Jumping events occurred in methane-water flow due to altered porosity caused by local contact in cleats.The distribution of residual phases changed the jumping direction on the micro-scale as well as the dominant channel on the mesoscale.Besides,jumping events caused additional energy dissipation,which was ignored in traditional two-phase flow models.This might contribute to the overestimation of relative permeability.The work provides new methods and insights for investigating unsaturated flow in complex porous media. 展开更多
关键词 Inter-cleat MICROFLUIDICS two-phase flow Dual-scale Interface jump Inertial effect
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An investigation of single-phased metallic solidification process using high-temperature confocal laser scanning microscope combined with differential scanning colorimetry
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作者 Xing-zhi Zhou De-yong Wang +6 位作者 Tian-peng Qu Dong Hou Shao-yan Hu Jun Tian Xiang-long Li Lei Fan Zhi-xiao Zhang 《Journal of Iron and Steel Research International》 2025年第2期437-451,共15页
To investigate the nucleation behavior during the single-phased metallic solidification process,the commercial ultrapure ferritic stainless steels with no(Initial steel)and various melt treatments(R1,MR1,Y2,MY1,and M1... To investigate the nucleation behavior during the single-phased metallic solidification process,the commercial ultrapure ferritic stainless steels with no(Initial steel)and various melt treatments(R1,MR1,Y2,MY1,and M1 steels)were used to carry out the differential scanning colorimetry(DSC)and high-temperature confocal laser scanning microscope(HT-CLSM)experiments.Based on the results of DSC experiments,the equilibrium solidification process as well as the relationship among the critical undercooling degree(△T_(c)^(DSC)),latent heat of fusion/crystallization(△H_(f)/△H_(c)),equiaxed grain ratio(ER),and average grain size(△_(ave)^(ingot))was revealed.ER is increased with the decreasing△T_(c)^(DSC)and increasing△H_(f)/△H_(c);however,△_(ave)^(ingot)is decreased with them.Referring to the results of HT-CLSM experiments,the average sizes of micro-/macrostructures(d_(ave)/D_(ave)/)are decreased with the increasing cooling rate,as well as the difference between and apparent critical undercooling degree(△T_(c)^(CLSM))was revealed.The heterogeneous nucleation of the crystal nuclei occurs only if△T_(c)^(CLSM)>△T_(c)^(DSC).Combining with the interfacial wetting-lattice mismatch heterogeneous nucleation model,the dynamic mechanism of the metallic solidification was revealed.The as-cast grains of the melt-treated samples were obviously refined,owing to the much higher actual heterogeneous nucleation rates(I_(heter.,i))obtained through melt treatments,and the heterogeneous nucleation rates(I_(heter.,ij))for all samples are increased with the cooling rates,firmly confirming that the as-cast grains of each sample could be refined by the increasing cooling rates. 展开更多
关键词 NUCLEATION Metallic solidification process Differential scanning colorimetry High-temperature confocal laser scanning microscope Interfacial wetting-lattice mismatch heterogeneous nucleation model
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The role of isolators in two-phase kerosene/air rotating detonation engines
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作者 Wenbo Cao Fang Wang +1 位作者 Chunsheng Weng Huangwei Zhang 《Defence Technology(防务技术)》 2025年第7期260-274,共15页
In this study, the three-dimensional non-premixed two-phase kerosene/air rotating detonation engines with different isolator configurations and throat area ratios are simulated by the Eulerian-Lagrangian method. The e... In this study, the three-dimensional non-premixed two-phase kerosene/air rotating detonation engines with different isolator configurations and throat area ratios are simulated by the Eulerian-Lagrangian method. The effects of the divergence, straight, and convergence isolators on the rotating detonation wave dynamics and the upstream oblique shock wave propagation mechanism are analyzed. The differences in the rotating detonation wave behaviors between ground and flight operations are clarified.The results indicate that the propagation regimes of the upstream oblique shock wave depend on the isolator configurations and operation conditions. With a divergence isolator, the airflow is accelerated throughout the isolator and divergence section, leading to a maximum Mach number(~1.8) before the normal shock. The total pressure loss reaches the largest, and the detonation pressure drops. The upstream oblique shock wave can be suppressed within the divergence section with the divergence isolator.However, for the straight and convergence isolators, the airflow in the isolator with a larger ψ_(1)(0.3 and0.4) can suffer from the disturbance of the upstream oblique shock wave. The critical incident angle is around 39° at ground operation conditions. The upstream oblique shock wave tends to be suppressed when the engine operates under flight operation conditions. The critical pressure ratio β_(cr0) is found to be able to help in distinguishing the propagation regimes of the upstream oblique shock wave. Slightly below or above the β_(cr0) can obtain different marginal propagation results. The high-speed airflow in the divergence section affects the fuel droplet penetration distance, which deteriorates the reactant mixing and the detonation area. Significant detonation velocity deficits are observed and the maximum velocity deficit reaches 26%. The results indicate the engine channel design should adopt different isolator configurations based on the purpose of total pressure loss or disturbance suppression. This study can provide useful guidance for the channel design of a more complete two-phase rotating detonation engine. 展开更多
关键词 Rotating detonation two-phase ISOLATOR Upstream oblique shock wave
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Efficient prediction of gaseous n-hexane removal in two-phase partitioning bioreactors with silicone oil based on the mechanism and kinetic models
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作者 Lichao Lu Tuo Ju +6 位作者 Yangdan Fang Jingtao Hu Zhuqiu Sun Zhuowei Cheng Qian Li Jianmeng Chen Dong-zhi Chen 《Journal of Environmental Sciences》 2025年第8期729-740,共12页
Two-phase partitioning bioreactors(TPPBs)have been widely used because they overcome the mass-transfer limitation of hydrophobic volatile organic compounds(VOCs)in waste gas biological treatments.Understanding the mec... Two-phase partitioning bioreactors(TPPBs)have been widely used because they overcome the mass-transfer limitation of hydrophobic volatile organic compounds(VOCs)in waste gas biological treatments.Understanding the mechanisms of mass-transfer enhancement in TPPBs would enable efficient predictions for further industrial applications.In this study,influences of gradually increasing silicone oil ratio on the TPPB was explored,and a 94.35%reduction of the n-hexane partition coefficient was observed with 0.1 vol.%silicone,which increased to 80.7%along with a 40-fold removal efficiency enhancement in the stabilised removal period.The elimination capacity increased from 1.47 to 148.35 g/(m^(3)·h),i.e.a 101-fold increase compared with that of the single-phase reactors,when 10 vol.%(3 Critical Micelle Concentration)silicone oil was added.The significantly promoted partition coefficient was the main reason for the mass transfer enhancement,which covered the negative influences of the decreased total mass-transfer coefficient with increasing silicone oil volume ratio.The gradually rising stirring rate was benefit to the n-hexane removal,which became negative when the dominant resistance shifted from mass transfer to biodegradation.Moreover,a mass-transfer-reaction kinetic model of the TPPB was constructed based on the balance of n-hexane concentration,dissolved oxygen and biomass.Similar to the mechanism,the partition factor was predicted sensitive to the removal performance,and another five sensitive parameters were found simultaneously.This forecasting method enables the optimisation of TPPB performance and provides theoretical support for hydrophobic VOCs degradation. 展开更多
关键词 Mass transfer N-HEXANE two-phase partitioning bioreactors Silicone oil
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A Review of Pressure Drop Characteristics and Optimization Measures of Two-Phase Flow with Low Boiling Point Working Fluids in Microchannels
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作者 Zongyu Jie Chao Dang Qingliang Meng 《Frontiers in Heat and Mass Transfer》 2025年第4期1053-1089,共37页
With the increasing miniaturization of systems and surging demand for power density,accurate prediction and control of two-phase flow pressure drop have become a core challenge restricting the performance of microchan... With the increasing miniaturization of systems and surging demand for power density,accurate prediction and control of two-phase flow pressure drop have become a core challenge restricting the performance of microchannel heat exchangers.Pressure drop,a critical hydraulic characteristic,serves as both a natural constraint for cooling systems and determines the power required to pump the working fluid through microchannels.This paper reviews the characteristics,prediction models,and optimization measures of two-phase flow pressure drop for low-boiling-point working fluids in microchannels.It systematically analyzes key influencing factors such as fluid physical properties,operating conditions,channel geometry,and flow patterns,and discusses the complex mechanisms of pressure drop under the coupling effect of multi-physical fields.Mainstream prediction models are reviewed:the homogeneous flow model simplifies calculations but shows large deviations at low quality;the separated flow model considers interphase interactions and can be applied to micro-scales after modification;the flow-pattern-based model performs zoned modeling but relies on subjective classification;machine learning improves prediction accuracy but faces the“black-box”problem.In terms of optimization,channel designs are improved through porous structures and micro-rib arrays,and flow rate distribution is optimized using splitters to balance pressure drop and heat transfer performance.This study provides theoretical support for microchannel thermal management in high-power-density devices. 展开更多
关键词 Pressure drop two-phase flow microchannels bubble shape prediction model
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Exosomes separation with aqueous two-phase systems from bovine milk
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作者 Jingjing Sun Ruoque Mao +2 位作者 Xiaoqian Fu Shanjing Yao Dongqiang Lin 《Chinese Journal of Chemical Engineering》 2025年第5期1-10,共10页
The exosomes hold significant potential in disease diagnosis and therapeutic interventions.The objective of this study was to investigate the potential of aqueous two-phase systems(ATPSs)for the separation of bovine m... The exosomes hold significant potential in disease diagnosis and therapeutic interventions.The objective of this study was to investigate the potential of aqueous two-phase systems(ATPSs)for the separation of bovine milk exosomes.The milk exosome partition behaviors and bovine milk separation were investigated,and the ATPSs and bovine milk whey addition was optimized.The optimal separation conditions were identified as 16%(mass)polyethylene glycol 4000,10%(mass)dipotassium phosphate,and 1%(mass)enzymatic hydrolysis bovine milk whey.During the separation process,bovine milk exosomes were predominantly enriched in the interphase,while protein impurities were primarily found in the bottom phase.The process yielded bovine milk exosomes of 2.0×10^(11)particles per ml whey with high purity(staining rate>90%,7.01×10^(10)particles per mg protein)and high uniformity(polydispersity index<0.03).The isolated exosomes were characterized and identified by transmission electron microscopy,zeta potential and size distribution.The results demonstrated aqueous two-phase extraction possesses a robust capability for the enrichment and separation of exosomes directly from bovine milk whey,presenting a novel approach for the large-scale isolation of exosomes. 展开更多
关键词 Aqueous two-phase extraction Bovine milk exosomes SEPARATION PURIFICATION
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Numerical investigation on the engraving process of a pyrotechnic actuator with an improved two-phase flow model of interior ballistic
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作者 Yue Li Cong Liu +1 位作者 Cheng Cheng Genghui Jiang 《Defence Technology(防务技术)》 2025年第4期120-132,共13页
By combining with an improved model on engraving process,a two-phase flow interior ballistic model has been proposed to accurately predict the flow and energy conversion behaviors of pyrotechnic actuators.Using comput... By combining with an improved model on engraving process,a two-phase flow interior ballistic model has been proposed to accurately predict the flow and energy conversion behaviors of pyrotechnic actuators.Using computational fluid dynamics(CFD),the two-phase flow and piston engraving characteristics of a pyrotechnic actuator are investigated.Initially,the current model was utilized to examine the intricate,multi-dimensional flow,and energy conversion characteristics of the propellant grains and combustion gas within the pyrotechnic actuator chamber.It was discovered that the combustion gas on the wall's constant transition from potential to kinetic energy,along with the combined effect of the propellant motion,are what create the pressure oscillation within the chamber.Additionally,a numerical analysis was conducted to determine the impact of various parameters on the pressure oscillation and piston motion,including pyrotechnic charge,pyrotechnic particle size,and chamber structural dimension.The findings show that decreasing the pyrotechnic charge will lower the terminal velocity,while increasing and decreasing the pyrotechnic particle size will reduce the pressure oscillation in the chamber.The pyrotechnic particle size has minimal bearing on the terminal velocity.The results of this investigation offer a trustworthy forecasting instrument for comprehending and creating pyrotechnic actuator designs. 展开更多
关键词 Pyrotechnic actuator Engraving process two-phase flow Pressure oscillation
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Modeling and Experimental Study of an Open Two-Phase Loop Driven by Osmotic Pressure and Capillary Force
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作者 Hanli Bi Zheng Peng +5 位作者 Chenpeng Liu Zhichao Jia Guoguang Li Yuandong Guo Hongxing Zhang Jianyin Miao 《Frontiers in Heat and Mass Transfer》 2025年第1期55-70,共16页
As space technology advances,thermal control systems must effectively collect and dissipate heat from distributed,multi-source environments.Loop heat pipe is a highly reliable two-phase heat transfer component,but it ... As space technology advances,thermal control systems must effectively collect and dissipate heat from distributed,multi-source environments.Loop heat pipe is a highly reliable two-phase heat transfer component,but it has several limitations when addressing multi-source heat dissipation.Inspired by the transport and heat dissipation system of plants,large trees achieve stable and efficient liquid supply under the influence of two driving forces:capillary force during transpiration in the leaves(pull)and root pressure generated by osmotic pressure in the roots(push).The root pressure provides an effective liquid supply with a driving force exceeding 2 MPa,far greater than the driving force in conventional capillary-pumped two-phase loops.Research has shown that osmotic heat pipes offer a powerful driving force,and combining osmotic pressure with capillary force has significant advantages.Therefore,this paper designs a multi-evaporator,dual-drive two-phase loop,using both osmotic pressure and capillary force to solve the multi-source heat dissipation challenge.First,a transmembrane water flux model for the osmotic pressure-driven device was established to predict the maximum heat transfer capacity of the dual-drive two-phase loop.Then,an experimental setup for a multi-evaporator“osmotic pressure+capillary force”dual-drive two-phase loop was constructed,capable of transferring at least 235 W of power under a reverse gravity condition of 20 m.The study also analyzed the effects of reverse gravity height,heat load distribution among the three evaporators,startup sequence,and varying branch resistances on the performance of the dual-drive two-phase loop. 展开更多
关键词 Multi-heat sources osmotic pressure two-phase loop dual-drive loop heat pipe
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Structural Optimization of Nozzles for Gas-Liquid Two-Phase Jets
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作者 Fengxia Shi Jian Zhao +3 位作者 Xiaodong Dai Guoxin Zhang Yuan Lu Yuyan Shang 《Fluid Dynamics & Materials Processing》 2025年第12期2963-2980,共18页
Gas–liquid two-phase jets exhibit markedly enhanced impact performance due to the violent collapse of entrained bubbles,which generates transient microjets and shock waves.The geometry of the nozzle is a decisive fac... Gas–liquid two-phase jets exhibit markedly enhanced impact performance due to the violent collapse of entrained bubbles,which generates transient microjets and shock waves.The geometry of the nozzle is a decisive factor in controlling jet formation,flow modulation,and impact efficiency.In this work,the structural optimization of gas–liquid two-phase nozzles was investigated numerically using the Volume of Fluid(VOF).Simulation results show that the aero-shaped nozzle delivers a significantly stronger impact on the target surface than conventional geometries.Specifically,its impact pressure is 21%higher than that of a conical straight nozzle and 37%higher than that of a conical nozzle.The aero nozzle not only increases peak impact pressure but also sustains it over a longer duration,leading to an overall improvement in energy transfer efficiency.Parametric analyses further reveal the key geometric conditions governing performance.When the nozzle curvature is set to 0.01,the jet achieves a higher and more stable surface pressure profile,maintaining elevated impact for a prolonged period.At an aspect ratio of 15,the jet exhibits pronounced pulsation under high pressure,thereby enhancing impact intensity.The contraction ratio exerts a non-monotonic influence:as it increases,impact pressure initially rises and subsequently declines,with an optimal value of 4 yielding the highest and most persistent impact pressure.Likewise,when the ratio of inlet length to outlet diameter is 2.5,the jet demonstrates the strongest impact on the target surface. 展开更多
关键词 Gas-liquid two-phase jet impact MODULATION nozzle optimization fluid volume method
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Numerical Simulation of Gas-Water Two-Phase Flow in a Proppant-Filled Layer
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作者 Jian Yang Xinghao Gou +4 位作者 Jiayi Sun Fei Liu Xiaojin Zhou Xu Liu Tao Zhang 《Fluid Dynamics & Materials Processing》 2025年第8期1935-1954,共20页
Shale gas production involves complex gas-water two-phase flow,with flow patterns in proppant-filled fractures playing a critical role in determining production efficiency.In this study,3D geometric models of 40/70 me... Shale gas production involves complex gas-water two-phase flow,with flow patterns in proppant-filled fractures playing a critical role in determining production efficiency.In this study,3D geometric models of 40/70 mesh ceramic particles and quartz sand proppant clusters were elaborated using computed tomography(CT)scanning.These models were used to develop a numerical simulation framework based on the lattice Boltzmann method(LBM),enabling the investigation of gas-water flow behavior within proppant-filled fractures under varying driving forces and surface tensions.Simulation results at a closure pressure of 15 MPa have revealed that ceramic particles exhibit a simpler and more porous internal structure than quartz sand of the same size.Under identical flow conditions,ceramic proppants demonstrate higher fluid replacement efficiency.Replacement efficiency increases with higher porosity,greater driving force,and lower surface tension.Furthermore,fluid displacement is strongly influenced by pore geometry:flow is faster in straighter and wider channels,with preferential movement through larger pores forming dominant flow paths.The replacement velocity exhibits a characteristic time evolution,initially rapid,then gradually decreasing,correlating positively with the development of these dominant channels. 展开更多
关键词 Proppant fractures gas-water two-phase flow numerical simulation lattice Boltzmann method flow behavior
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A layer-specific constraint-based enriched physics-informed neural network for solving two-phase flow problems in heterogeneous porous media
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作者 Jing-Qi Lin Xia Yan +4 位作者 Er-Zhen Wang Qi Zhang Kai Zhang Pi-Yang Liu Li-Ming Zhang 《Petroleum Science》 2025年第11期4714-4735,共22页
In this study,we propose a constraint learning strategy based on interpretability analysis to improve the convergence and accuracy of the enriched physics-informed neural network(EPINN),which is applied to simulate tw... In this study,we propose a constraint learning strategy based on interpretability analysis to improve the convergence and accuracy of the enriched physics-informed neural network(EPINN),which is applied to simulate two-phase flow in heterogeneous porous media.Specifically,we first analyze the layerwise outputs of EPINN,and identify the distinct functions across layers,including dimensionality adjustment,pointwise construction of non-equilibrium potential,extraction of high-level features,and the establishment of long-range dependencies.Then,inspired by these distinct modules,we propose a novel constraint learning strategy based on regularization approaches,which improves neural network(NN)learning through layer-specific differentiated updates to enhance cross-timestep generalization.Since different neu ral network layers exhibit varying sensitivities to global generalization and local regression,we decrease the update frequency of layers more sensitive to local learning under this constraint learning strategy.In other words,the entire neural network is encouraged to extract more generalized features.The superior performance of the proposed learning strategy is validated through evaluations on numerical examples with varying computational complexities.Post hoc analysis reveals that gradie nt propagation exhibits more pronounced staged characte ristics,and the partial differential equation(PDE)residuals are more uniformly distributed under the constraint guidance.Interpretability analysis of the adaptive constraint process suggests that maintaining a stable information compression mode facilitates progressive convergence acceleration. 展开更多
关键词 Physics-informed learning Explainable artificial intelligence Constraint learning two-phase flow Heterogeneous porous media
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Robust,High-Magnetic-Field-Resistance Design of Scanning Head Equipped with 12 T Superconductor Magnet and sub-Kelvin He-3 Cryostat
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作者 Junwei Liu Kesen Zhao +5 位作者 Aile Wang Min Zhang Jihao Wang Yubin Hou Wenjie Meng Qingyou Lu 《Chinese Journal of Chemical Physics》 2025年第6期855-864,I0238,I0239,共12页
We have constructed the robust and highfield-stability scanning tunneling microscopy(STM)system which is equipped with a 12 Tsuperconductor magnet and the He-3 cryostat,obtaining the high-quality data of graphite whic... We have constructed the robust and highfield-stability scanning tunneling microscopy(STM)system which is equipped with a 12 Tsuperconductor magnet and the He-3 cryostat,obtaining the high-quality data of graphite which can be clearly characterized up to the 12-T high magnetic field and bulk 2H-NbSe2 whose vortex structure and surface defects can be clearly visualized by the scanning and dI/dV mapping.The pivotal factor of our superior data stems from the sapphire frame and its unique structure,which ensure an exceptional performance for the noise,which has the current spectral density low to 2×10^(-13)A√Hz at the low frequency with the cut-off frequency of 250 Hz and the engaging status,of vibration,promising to the robust platform for the investigation of the surface of materials.The low drifting rate from the image of graphite is v_(x)=-8:79×10^(-7)nm/s and v_(y)=-3.26×10^(-6)nm/s at 12 T and 0.3 K.Besides,equipped with the high-capacity He-3 cryostat(with 15 normal temperature and pressure liter He-3 gas),the system,which has the base temperature for 0.28 K,can operate at the low temperature for more than 72 h,ensuring the enduring research at the sub-Kelvin temperature for the dI/dV mapping and large-scale scanning.When the magnetic field is applied up to 12 T,the STM system also obtains the excellent-clarity and undistorted images of graphite,which proves the outstanding performance of the system at the high-field researches. 展开更多
关键词 sub-Kelvin High magnetic field Low noise High-stability scanning tunneling microscopy
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