The flower of Syringa pubescens Turcz.(SP),was used as both medicine and food in China,exhibited various biological activities.However,it remains unknown whether SP affects ameliorative nonalcoholic steatohepatitis(NA...The flower of Syringa pubescens Turcz.(SP),was used as both medicine and food in China,exhibited various biological activities.However,it remains unknown whether SP affects ameliorative nonalcoholic steatohepatitis(NASH).In this study,the improvement of SP ethyl acetate extract(SPE)on NASH was evaluated and the potential mechanisms were explored.The glycosides of SPE were determined by HPLC method.Hep G2 cell lines were treated with free fatty acid to clarify the improvement effect and mechanisms of SPE on NASH in vitro.C57BL/6J mice were treated by 60%kcal high-fat diet(HFD)combined with carbon tetrachloride(CCl_4)to establish the NASH model in vivo.The results showed SPE could inhibit the hepatic injury and lipid steatosis by decreasing the levels of alanine aminotransferase,aspartate aminotransferase,triglyceride and total cholesterol in vitro and in vivo.The SPE suppressed the oxidative stress and inflammation by restraining the generation of reactive oxygen species,malondialdehyde,interleukin(IL)-1β,IL-6 and tumor necrosis factor-α(TNF-α)and enhancing the activity of antioxidant enzymes including superoxide dismutase,catalase and glutathione peroxidase in NASH model.Moreover,SPE declined the level of fibrosis markers including hydroxyproline,type I collagen andα-smooth muscle actin(α-SMA)in HFD combined CCl_4-induced mice.Mechanically,SPE inhibited hepatocellular Kelch like ECH associated protein 1 expression and promoted nuclear erythroid 2-related factor 2(Nrf2)nuclear translocation,which suppressed the phosphorylation of IκBα/NF-κB.In addition,SPE down-regulated the level of transforming growth factorβ1(TGF-β1)and phosphorylation of Smad2 to mitigate fibrosis.In brief,SPE could significantly alleviate lipid accumulation,oxidative stress,inflammation and fibrosis via regulating Nrf2/HO-1,IκBα/NF-κB and TGF-β1/Smad2 pathways in the progression of NASH,which indicated that SPE had the potential to be a novel and effective drug or food supplements for the improvement of NASH.展开更多
[Objectives]To explore the action mechanism of Mongolian medicine Rhododendron micranthum Turcz.on lung cancer by network pharmacology.[Methods]Based on the high-throughput experiment and reference database(HERB)of tr...[Objectives]To explore the action mechanism of Mongolian medicine Rhododendron micranthum Turcz.on lung cancer by network pharmacology.[Methods]Based on the high-throughput experiment and reference database(HERB)of traditional Chinese medicine,component target database(Swiss ADME),small molecule drug target prediction online platform(SWISS Target Prediction),human gene business card database(GENECARD),the database of genes and mutation sites related to human diseases(DISGENET)and other databases,the target genes of drugs and diseases were screened out.Venny software was used for obtaining the target intersection of active components of the Mongolian medicine R.micranthum Turcz.and the lung cancer,a CytoNCA plug-in in cytoscape 3.10.0 software was used for screening candidate core target genes,and related effective components were obtained in a reverse direction.A drug-active ingredient-gene-disease regulation network was established,a protein-protein interaction(PPI)network was established by means of the STRING database to screen core genes,and common targets were screened by the David database.Gene Ontology(GO)function and Kyoto Encyclopedia of Genes and Genomes(KEGG)were used for enrichment analysis.[Results]There were 13 effective components of Mongolian medicine R.micranthum Turcz.for treating lung cancer and 115 drug disease intersection target genes.Core genes affecting the disease included SRC,HSP90AB1,EGFR,AKT1,and ERBB2.GO functional enrichment analysis involved 462 items of biological processes,64 items of cellular components and 126 items of molecular functions.Enrichment analysis of KEGG signaling pathways screened out cancer pathways,endocrine resistance,PI3K-Akt signaling pathways,proteoglycans in cancer and other signaling pathways.[Conclusions]Mongolian medicine R.micranthum Turcz.can inhibit the proliferation of lung cancer cells from multiple targets and pathways,and the results of network pharmaceutical analysis provide a theoretical basis for further experimental research.展开更多
[Objective] The aim of this study is to establish the rapid micro-propagation system in Thesium chinense Turcz.[Method]With stem fragments of wild Thesium chinense Turcz as explants,different culture media were design...[Objective] The aim of this study is to establish the rapid micro-propagation system in Thesium chinense Turcz.[Method]With stem fragments of wild Thesium chinense Turcz as explants,different culture media were designed to conduct induction culture,strengthening plantlet culture and in vitro rooting.[Result]The optimum medium for inducing clustered shoots was determined to be MS medium appended with 1.5 mg/L 6-BA,0.01 mg/L NAA and 0.3 mg/L 2,4-D;in addition,60 mg/kg ABT was suitable for rooting,by which the percentage of rooted plantlets reached 76.6%.[Conclusion]This study simplified the procedures of tissue culture in Thesium chinense Turcz and enhanced the proliferation rate,providing basis for artificial cultivation and resource protection of Thesium chinense Turcz.展开更多
Phytochemical investigation of the leaves of Premna microphylla Turcz led to the isolation of 13 known compounds. Based on spectroscopic and chemical evidences, their structures were identified as diosmetin （1）, blu...Phytochemical investigation of the leaves of Premna microphylla Turcz led to the isolation of 13 known compounds. Based on spectroscopic and chemical evidences, their structures were identified as diosmetin （1）, blumenol A （2）, （3S,5R,6S,7E,9R）-5,6-epoxy-3,9-dihydroxy-7-megastigmene （3）, 3β-hydroxy-5a,6a-epoxy-γ-megastigmen-9-one （4）, ixerol B （5）, （-）-dehydrovomifoliol （6）, 3S,5R-dihydroxy-6S,7-megastigmadien-9-one （7）, loliotide （8）, （＋）-dehydrololiolide （9）, （＋）-medioresinol （10）, 4-oxopinoresinol （11）, tormentic acid （12）, and indole-3-carboxylic acid （13）. Compounds 2-13 described above were isolated from this genus for the first time.展开更多
[Objective] This paper aimed to study the tissue culture and rapid propagation technology of superior individuals of Lonicera edulis Turcz. [Method] Several superior individuals of Lonicera edulis Turcz were used as m...[Objective] This paper aimed to study the tissue culture and rapid propagation technology of superior individuals of Lonicera edulis Turcz. [Method] Several superior individuals of Lonicera edulis Turcz were used as materials for selecting the primary medium, subculture medium, rooting medium and acclimatization substrate during the tissue culture and rapid propagation. [Result] 6-BA was the optimal cytokinin for tissue culture of Lonicera edulis Turcz, compared with ZT; modified MS＋1.0 mg/L of 6-BA ＋ 0.2 mg/L of IBA was the optimal medium as primary and subculture medium, modified MS＋ 1.5 mg/L of IBA was the optimal medium for rooting of Lonicera edulis Turcz, the rooting rate had achieved 100% after cultured for 30 d. The optimal substrate for transplanting plantlets of Lonicera edulis Turcz was composed of humus and perlite （1∶ 1, V/V）, survival rate was as high as 95% after 30 d. [Conclusion] This study provided basis for the rapid propagation of superior seedlings of Lonicera edulis Turcz, as well as the establishment of industrialized breeding technical system and the implementation of scale production.展开更多
[Objective] This study was to reveal the anatomic structure of Premna microphylla Turcz.stem and leaf.[Method] Using plant anatomy method,we performed anatomic analysis of Premna microphylla Turcz.stem and leaf.[Resul...[Objective] This study was to reveal the anatomic structure of Premna microphylla Turcz.stem and leaf.[Method] Using plant anatomy method,we performed anatomic analysis of Premna microphylla Turcz.stem and leaf.[Result] The stem of Premna microphylla Turcz.is axially symmetric organ,its primary structure consists of three parts of epidermis,cortex and vascular cylinder.The petiole consists of epidermis,cortex and vascular cylinder,of which epidermis is a layer of cells with cuticle and epidermal hair.The sun leaf in Premna microphylla is dorsi-ventral leaf,wich is consist of palisade tissue and spongy tissue;while its shade leaf of Premna microphylla almost are isobilateral leaf.[Conclusion] Our results provided reference for further the exploitation and utilization of Premna microphylla Turcz.resources.展开更多
This study was to investigate the decoloration effects of activated clay, activated carbon, diatomite, Ca group bentonite, kaolin, activated aluminum oxide, magnesium oxide and attapulgite. Ultraviolet-visible spectro...This study was to investigate the decoloration effects of activated clay, activated carbon, diatomite, Ca group bentonite, kaolin, activated aluminum oxide, magnesium oxide and attapulgite. Ultraviolet-visible spectroscopywas adopted to de- tect the absorbanees of the extracts of Schisandra chinensis fruit and rattan stems, the contents of total triterpenoids in S. chinensis fruit and rattan stems were deter- mined, and the decoloration rate and the retention rate of total triterpenoids of S. chi- nensis fruit and rattan stems were calculated. The results indicated that, attapulgite exhibited the best decoloration effect on the extract of S. chinensis fruit, with a de- coloration rate up to 60.47%, activated carbon exhibited a better decoloration effect on the extract of S. chinensis rattan stems, with a decoloration rate up to 69.24%, and they had relatively higher retention rates of total triterpenoids. In the eight de- colorants, attapulgite showed the best decoloration effect on the extract of S. chi- nensis fruit, and activated carbon exhibited the best decoloration effect on the ex- tract of S. chinensisrattan stems.展开更多
The study aimed to optimize the induction and differentiation medium by exploreing different tissue culture of Saposhnikovia divaricata （Turcz.） Schischk. In tissue culture with the root, stem segments, young leaf, ...The study aimed to optimize the induction and differentiation medium by exploreing different tissue culture of Saposhnikovia divaricata （Turcz.） Schischk. In tissue culture with the root, stem segments, young leaf, cotyledonary node and axillary bud of Saposhnikovia divaricata （Turcz.） Schischk as explants, a lot of plantleles were obtained and the corresponding plant regeneration-system was established. The results showed that when use MS＋1.0 mg·L^-1 6-BA＋0.2 mg·L^-1 NAA as callus induction medium, the cotyledonary node had the highest bourgeon rate, and its callus was better than any others; MS＋2 mg·L^-1 6-BA＋0.4 mg·L^-1 NAA was the best adventitious buds induction medium, and the best adventitious buds induced condition was 3% sucrose as carbon source, illumination for 12-14 h·d^-1 and pH 5.8, The best rootage medium was 1/2 MS＋0.5 mg·L^-1 NAA.展开更多
We investigated the diversity and composition of microflora in feces of Lycopus lucidus Turcz.-fed mice.In addition,we evaluated the production of major cytokines(Interleukin-6 and-10)which are related to inflammation...We investigated the diversity and composition of microflora in feces of Lycopus lucidus Turcz.-fed mice.In addition,we evaluated the production of major cytokines(Interleukin-6 and-10)which are related to inflammation and fatty acid composition of several tissues.16S ribosomal DNA sequencing-based microbiome taxonomic profiling analysis was performed utilizing the EzBioCloud data base.Male mice fed on L.lucidus showed a significantly reduced number of lactic acid bacteria and coliform in the feces compared with the control group(p<0.05).16S rDNA sequencing analysis of fecal samples showed that L.lucidus supplementation decreased the community of harmful microflora(Enterobacteriaceae including Escherichia coli and Bacteroides sp.)in feces compared with the control group(p<0.05).There were no significant differences in mRNA expression of cytokine IL-6 and IL-10 between the control and L.lucidus fed groups.The fecal fatty acid composition in the L.lucidus group had percentages of 4:0,6:0,8:0 and 10:0 in the intestine but those short chain fatty acids were not detected in the control group.Our results showed that L.lucidus supplementation influenced gut environment by decreasing harmful microflora and increased the percentages of several short fatty acids.展开更多
Objective:The fruit stalk of Schisandra chinensis(Turcz.)Baill.(S.chinensis)has been found to contain bioactive components similar to the fruit of S.chinensis.Here,we report a recent discovery about new nortriterpenoi...Objective:The fruit stalk of Schisandra chinensis(Turcz.)Baill.(S.chinensis)has been found to contain bioactive components similar to the fruit of S.chinensis.Here,we report a recent discovery about new nortriterpenoids with a novel skeleton and anti-gastric cancer activity,which were isolated from the fruit stalk of S.chinensis.Methods:The chemical components of ethyl acetate extract from 70%ethanol extract from S.chinensis fruit stalk were separated,purified,and identified by liquid chromatography methods(silica gel,ODS,HPLC)and extensive spectroscopic analyses(NMR,IR,UV,MS,CD).Results:Two new nortriterpenoids,schilancitrilactone M and 25-hydroxyl schindilactone D(1 and 2),along with ten known nortriterpenoids(3-12)were isolated from the fruit stalk of S.chinensis.The isolated compounds were tested for their cytotoxic activities against MGC-803 cells,and the results showed that compounds 6-8 possessed significant activities with IC50 of 9.01,11.77,and 2.74μmol/L,respectively.Conclusion:Twelve nortriterpenoids including two new compounds were isolated from the fruit stalk of S.chinensis for the first time.Among them,compounds 6-8 showed significant anti-gastric cancer activities.We postulated that the fruit stalk of S.chinensis could be used as an anti-gastric cancer drug.展开更多
[Objectives]The research aimed to optimize the extraction method of triterpenoids from A. selengnesis. [Methods]The ultrasonic extraction of triterpenoids in A. selengnesis was optimized by single factor analysis( inc...[Objectives]The research aimed to optimize the extraction method of triterpenoids from A. selengnesis. [Methods]The ultrasonic extraction of triterpenoids in A. selengnesis was optimized by single factor analysis( including infusion time,ethanol concentration,ultrasonic power,extraction time,and liquid-solid ratio) and response surface method. [Results]The optimum extracting process of triterpenoids was as follows: infusion time of 65 min,ethanol concentration of 80%,ultrasonic power of 275 W,extraction time of 30 min,and liquid-solid ratio of 34 m L/g. Under the optimized condition,the extraction content of triterpenoids reached( 18. 26 ± 0. 10) mg/g. [Conclusions] The optimized extraction had a good modal on practice in developing and utilization.展开更多
Schisandra chinensis (Turcz.) Baill. is a deciduous woody vine, and belongs to the Schisandraceae family. This species is a horticultural plant with edible fruit. It is used medicinally and for making rope. It is dist...Schisandra chinensis (Turcz.) Baill. is a deciduous woody vine, and belongs to the Schisandraceae family. This species is a horticultural plant with edible fruit. It is used medicinally and for making rope. It is distributed in Hebei, Heilongjiang, Jilin, Liaoning, Nei Mongol, Shanxi展开更多
The qualitative characterization and quantitative analysis of five bioactive flavonoids in Salix bordensis Turcz. were achieved via reversed-phase high-performance liquid chromatography coupled with diode array detect...The qualitative characterization and quantitative analysis of five bioactive flavonoids in Salix bordensis Turcz. were achieved via reversed-phase high-performance liquid chromatography coupled with diode array detection and tandem mass spectrometry, by using an Agilent ZORBAX SB-C18 HPLC column with a gradient elution of 0.3% (v/v) formic acid in water and methanol as the mobile phase. The compounds in the mixture were clearly identified by comparing their HPLC-DAD ultraviolet spectra, retention times, and MS data with those of corresponding reference compounds. All calibration curves showed good linearity (r2 > 0.9998) within the test ranges. The LOD, LOQ, specificity, precision, and accuracy for the method were validated. The results demonstrated that this analytical approach is ideal for the determination of bioactive compounds, such as flavonoids, and that it constructed a basis for the comprehensive evaluation of the quality of Salix bordensis Turcz.展开更多
The main components of Artemisia leucophylla(Turcz.ex Bess.) C.B.Clarke extract are volatile oils,flavonoids,lignans,terpenoids,etc.Studies have shown that A.leucophylla has bactericidal,anti-inflammatory,anti-oxidant...The main components of Artemisia leucophylla(Turcz.ex Bess.) C.B.Clarke extract are volatile oils,flavonoids,lignans,terpenoids,etc.Studies have shown that A.leucophylla has bactericidal,anti-inflammatory,anti-oxidant,anti-ultraviolet functions,and it has large development space and application value in many fields.This paper first introduced the growth resource distribution,active component extraction method,composition and efficacy of A.leucophylla,and analyzed the application prospects in daily necessities,in order to provide reference for the in-depth research,development and utilization of A.leucophylla.展开更多
[ Objectives] This study was conducted to provide high-quality polyploid for returning the grain plots to forestry and promote the rapid development of Lonicera edulis Turcz. [ Methods] With superior individuals of wi...[ Objectives] This study was conducted to provide high-quality polyploid for returning the grain plots to forestry and promote the rapid development of Lonicera edulis Turcz. [ Methods] With superior individuals of wild L. edulis in Changbai Mountains as experimental materials and colchicine as inducer, polyploid induction was performed twice on diploid plants by addition method, and 7 polyploidy plants were identified from 11 regenerated plantlets. [Results] Among the 7 polyploidy plants, four materials were tetraploid, the concentration and treatment time of which were 300 mg/L and 7 and 14 d, respectively; and three materials were octaploid, the concentrations and treatment time of which were 300 and 500 mg/L and 14 d, respectively. [ Conclusions] The results of this study showed that colchicine had a higher polyploid induction rate for L. edulis with lower toxic and side effects, and the polyploidy plants could restore to normal growth state after several times of cubculture. Therefore, colchicine is an polyploid inducer suitable for L. edulis.展开更多
Corydalis bungeana Turcz.(CB)is a medicinal herb with significant medicinal value in traditional Chinese medicine.This paper reviews the progress of research on CB’s botany,quality control,phytochemistry,pharmacology...Corydalis bungeana Turcz.(CB)is a medicinal herb with significant medicinal value in traditional Chinese medicine.This paper reviews the progress of research on CB’s botany,quality control,phytochemistry,pharmacology and toxicity.The plant’s information was gathered from scientific databases such as PubMed,GeenMedical,Springer Link(https://link.springer.com),Chinese National Knowledge Infrastructure,Pharmacopoeia and Flora.Currently,137 phytochemicals have been identified and isolated from CB,including alkaloids,flavonoids,amino acids,terpenoids,coumarins and organic acids.In addition,many phytochemicals reported various antiinflammatory,antibacterial,antiviral,antitumor,analgesic,hepatoprotective,immunomodulatory,neuromodulatory,and lipid reduction activities.However,the study of its toxicity is still at the preliminary exploration stage and needs further intensive exploration.Herein,we provide an in-depth investigation of the progress of CB to elucidate the underlying mechanisms of activity of CB extracts and its major components,deliver valuable resources and information for further research and rational drug use,and explore the potential research directions and prospects of CB.展开更多
Objective: To study the effects of n-butanol extract of Rumex gmelini Turcz on the endotoxin-induced acute lung injury (ALI) in mice. Methods: Kunming mice were selected as experimental animals and randomly divided in...Objective: To study the effects of n-butanol extract of Rumex gmelini Turcz on the endotoxin-induced acute lung injury (ALI) in mice. Methods: Kunming mice were selected as experimental animals and randomly divided into normal control group (NC group), acute lung injury group (ALI group) and n-butanol extract of Rumex gmelini Turcz group (Rum group). ALI group and Rum group were established into ALI models by intraperitoneal injection of endotoxin, and Rum group were given intragastric administration of n-butanol extract of Rumex gmelini Turcz for intervention before model establishment. 12 h after endotoxin injection, the superior lobe of right lung was taken to determine the water content, and the inferior lobe of right lung was taken to determine the contents of AQPs molecules, inflammatory response molecules and oxidative stress molecules. Results: 12 h after endotoxin injection, the water content of lung tissue in Rum group was (6.82±0.97)%. After variance analysis, the water content of lung tissue in ALI group was significantly higher than that in NC group, AQP1 and AQP5 protein levels in lung tissue were significantly lower than those of NC group, AQP3 and AQP4 protein levels were not different from those of NC group, and MPO, NF-kB, TNF-α, HMGB1, IL-8, ROS, ATP, MDA, Bax and Caspase-3 protein levels were significantly higher than those of NC group;the water content of lung tissue in Rum group was significantly lower than that in ALI group, AQP1 and AQP5 protein levels in lung tissue were significantly higher than those of ALI group, AQP3 and AQP4 protein levels were not different from those of ALI group, and MPO, NF-kB, TNF-α, HMGB1, IL-8, ROS, ATP, MDA, Bax and Caspase-3 protein levels were significantly lower than those of ALI group. Conclusion:The n-butanol extract of Rumex gmelini Turcz reduce the pulmonary edema, inflammatory response, oxidative stress and apoptosis during the endotoxin-induced ALI in mice.展开更多
基金supported by grants from the National Natural Science Foundation of China(U1804175,32270418)the Joint Fund of Provincial Science and Technology Research and Development plan of Henan Province(232103810055,232301420100)。
文摘The flower of Syringa pubescens Turcz.(SP),was used as both medicine and food in China,exhibited various biological activities.However,it remains unknown whether SP affects ameliorative nonalcoholic steatohepatitis(NASH).In this study,the improvement of SP ethyl acetate extract(SPE)on NASH was evaluated and the potential mechanisms were explored.The glycosides of SPE were determined by HPLC method.Hep G2 cell lines were treated with free fatty acid to clarify the improvement effect and mechanisms of SPE on NASH in vitro.C57BL/6J mice were treated by 60%kcal high-fat diet(HFD)combined with carbon tetrachloride(CCl_4)to establish the NASH model in vivo.The results showed SPE could inhibit the hepatic injury and lipid steatosis by decreasing the levels of alanine aminotransferase,aspartate aminotransferase,triglyceride and total cholesterol in vitro and in vivo.The SPE suppressed the oxidative stress and inflammation by restraining the generation of reactive oxygen species,malondialdehyde,interleukin(IL)-1β,IL-6 and tumor necrosis factor-α(TNF-α)and enhancing the activity of antioxidant enzymes including superoxide dismutase,catalase and glutathione peroxidase in NASH model.Moreover,SPE declined the level of fibrosis markers including hydroxyproline,type I collagen andα-smooth muscle actin(α-SMA)in HFD combined CCl_4-induced mice.Mechanically,SPE inhibited hepatocellular Kelch like ECH associated protein 1 expression and promoted nuclear erythroid 2-related factor 2(Nrf2)nuclear translocation,which suppressed the phosphorylation of IκBα/NF-κB.In addition,SPE down-regulated the level of transforming growth factorβ1(TGF-β1)and phosphorylation of Smad2 to mitigate fibrosis.In brief,SPE could significantly alleviate lipid accumulation,oxidative stress,inflammation and fibrosis via regulating Nrf2/HO-1,IκBα/NF-κB and TGF-β1/Smad2 pathways in the progression of NASH,which indicated that SPE had the potential to be a novel and effective drug or food supplements for the improvement of NASH.
基金Supported by Inner Mongolia Autonomous Region Department of Education Science and Technology Leading Talents and Innovation Team Building Project and Inner Mongolia Natural Science Foundation Project(2024FX36)Key Research Project of Science and Technology in Colleges and Universities of Inner Mongolia Autonomous Region(NJZZ21029).
文摘[Objectives]To explore the action mechanism of Mongolian medicine Rhododendron micranthum Turcz.on lung cancer by network pharmacology.[Methods]Based on the high-throughput experiment and reference database(HERB)of traditional Chinese medicine,component target database(Swiss ADME),small molecule drug target prediction online platform(SWISS Target Prediction),human gene business card database(GENECARD),the database of genes and mutation sites related to human diseases(DISGENET)and other databases,the target genes of drugs and diseases were screened out.Venny software was used for obtaining the target intersection of active components of the Mongolian medicine R.micranthum Turcz.and the lung cancer,a CytoNCA plug-in in cytoscape 3.10.0 software was used for screening candidate core target genes,and related effective components were obtained in a reverse direction.A drug-active ingredient-gene-disease regulation network was established,a protein-protein interaction(PPI)network was established by means of the STRING database to screen core genes,and common targets were screened by the David database.Gene Ontology(GO)function and Kyoto Encyclopedia of Genes and Genomes(KEGG)were used for enrichment analysis.[Results]There were 13 effective components of Mongolian medicine R.micranthum Turcz.for treating lung cancer and 115 drug disease intersection target genes.Core genes affecting the disease included SRC,HSP90AB1,EGFR,AKT1,and ERBB2.GO functional enrichment analysis involved 462 items of biological processes,64 items of cellular components and 126 items of molecular functions.Enrichment analysis of KEGG signaling pathways screened out cancer pathways,endocrine resistance,PI3K-Akt signaling pathways,proteoglycans in cancer and other signaling pathways.[Conclusions]Mongolian medicine R.micranthum Turcz.can inhibit the proliferation of lung cancer cells from multiple targets and pathways,and the results of network pharmaceutical analysis provide a theoretical basis for further experimental research.
基金Supported by the Key Project for Supporting New Subject from Anhui Academy of Agricultural Sciences~~
文摘[Objective] The aim of this study is to establish the rapid micro-propagation system in Thesium chinense Turcz.[Method]With stem fragments of wild Thesium chinense Turcz as explants,different culture media were designed to conduct induction culture,strengthening plantlet culture and in vitro rooting.[Result]The optimum medium for inducing clustered shoots was determined to be MS medium appended with 1.5 mg/L 6-BA,0.01 mg/L NAA and 0.3 mg/L 2,4-D;in addition,60 mg/kg ABT was suitable for rooting,by which the percentage of rooted plantlets reached 76.6%.[Conclusion]This study simplified the procedures of tissue culture in Thesium chinense Turcz and enhanced the proliferation rate,providing basis for artificial cultivation and resource protection of Thesium chinense Turcz.
基金National Natural Science Foundation of China(Grant No.31270390)Program for New Century Excellent Talents in University(Grant No.NCET-08-0224)
文摘Phytochemical investigation of the leaves of Premna microphylla Turcz led to the isolation of 13 known compounds. Based on spectroscopic and chemical evidences, their structures were identified as diosmetin （1）, blumenol A （2）, （3S,5R,6S,7E,9R）-5,6-epoxy-3,9-dihydroxy-7-megastigmene （3）, 3β-hydroxy-5a,6a-epoxy-γ-megastigmen-9-one （4）, ixerol B （5）, （-）-dehydrovomifoliol （6）, 3S,5R-dihydroxy-6S,7-megastigmadien-9-one （7）, loliotide （8）, （＋）-dehydrololiolide （9）, （＋）-medioresinol （10）, 4-oxopinoresinol （11）, tormentic acid （12）, and indole-3-carboxylic acid （13）. Compounds 2-13 described above were isolated from this genus for the first time.
基金Supported by the National Natural Science Fundation of China(30960231)Project of the State Forestry Administration(2005-06)~~
文摘[Objective] This paper aimed to study the tissue culture and rapid propagation technology of superior individuals of Lonicera edulis Turcz. [Method] Several superior individuals of Lonicera edulis Turcz were used as materials for selecting the primary medium, subculture medium, rooting medium and acclimatization substrate during the tissue culture and rapid propagation. [Result] 6-BA was the optimal cytokinin for tissue culture of Lonicera edulis Turcz, compared with ZT; modified MS＋1.0 mg/L of 6-BA ＋ 0.2 mg/L of IBA was the optimal medium as primary and subculture medium, modified MS＋ 1.5 mg/L of IBA was the optimal medium for rooting of Lonicera edulis Turcz, the rooting rate had achieved 100% after cultured for 30 d. The optimal substrate for transplanting plantlets of Lonicera edulis Turcz was composed of humus and perlite （1∶ 1, V/V）, survival rate was as high as 95% after 30 d. [Conclusion] This study provided basis for the rapid propagation of superior seedlings of Lonicera edulis Turcz, as well as the establishment of industrialized breeding technical system and the implementation of scale production.
基金Supported by the Supporting Program of the"Eleventh Five-year Plan"for Sci&Tech Research of China(2007BAD87B06)Major Fund of Anhui Province Education Department(KJ2010A114)~~
文摘[Objective] This study was to reveal the anatomic structure of Premna microphylla Turcz.stem and leaf.[Method] Using plant anatomy method,we performed anatomic analysis of Premna microphylla Turcz.stem and leaf.[Result] The stem of Premna microphylla Turcz.is axially symmetric organ,its primary structure consists of three parts of epidermis,cortex and vascular cylinder.The petiole consists of epidermis,cortex and vascular cylinder,of which epidermis is a layer of cells with cuticle and epidermal hair.The sun leaf in Premna microphylla is dorsi-ventral leaf,wich is consist of palisade tissue and spongy tissue;while its shade leaf of Premna microphylla almost are isobilateral leaf.[Conclusion] Our results provided reference for further the exploitation and utilization of Premna microphylla Turcz.resources.
基金Supported by Key Science and Technology Research and Development Program of Jilin Province(20140204068YY,20140204062YY)Special Fund for Pharmaceutical Industry Development of Jilin Province(YYZW201246)~~
文摘This study was to investigate the decoloration effects of activated clay, activated carbon, diatomite, Ca group bentonite, kaolin, activated aluminum oxide, magnesium oxide and attapulgite. Ultraviolet-visible spectroscopywas adopted to de- tect the absorbanees of the extracts of Schisandra chinensis fruit and rattan stems, the contents of total triterpenoids in S. chinensis fruit and rattan stems were deter- mined, and the decoloration rate and the retention rate of total triterpenoids of S. chi- nensis fruit and rattan stems were calculated. The results indicated that, attapulgite exhibited the best decoloration effect on the extract of S. chinensis fruit, with a de- coloration rate up to 60.47%, activated carbon exhibited a better decoloration effect on the extract of S. chinensis rattan stems, with a decoloration rate up to 69.24%, and they had relatively higher retention rates of total triterpenoids. In the eight de- colorants, attapulgite showed the best decoloration effect on the extract of S. chi- nensis fruit, and activated carbon exhibited the best decoloration effect on the ex- tract of S. chinensisrattan stems.
基金Supported by Natural Science Foundation of Heilongjiang Province (C2005-31)
文摘The study aimed to optimize the induction and differentiation medium by exploreing different tissue culture of Saposhnikovia divaricata （Turcz.） Schischk. In tissue culture with the root, stem segments, young leaf, cotyledonary node and axillary bud of Saposhnikovia divaricata （Turcz.） Schischk as explants, a lot of plantleles were obtained and the corresponding plant regeneration-system was established. The results showed that when use MS＋1.0 mg·L^-1 6-BA＋0.2 mg·L^-1 NAA as callus induction medium, the cotyledonary node had the highest bourgeon rate, and its callus was better than any others; MS＋2 mg·L^-1 6-BA＋0.4 mg·L^-1 NAA was the best adventitious buds induction medium, and the best adventitious buds induced condition was 3% sucrose as carbon source, illumination for 12-14 h·d^-1 and pH 5.8, The best rootage medium was 1/2 MS＋0.5 mg·L^-1 NAA.
基金This research was supported by Basic Science Research Program of the National Research Foundation of Korea(NRF)in the Ministry of ScienceICT and Future Planning(NRF-2017R1A2B4005915)supported this research.
文摘We investigated the diversity and composition of microflora in feces of Lycopus lucidus Turcz.-fed mice.In addition,we evaluated the production of major cytokines(Interleukin-6 and-10)which are related to inflammation and fatty acid composition of several tissues.16S ribosomal DNA sequencing-based microbiome taxonomic profiling analysis was performed utilizing the EzBioCloud data base.Male mice fed on L.lucidus showed a significantly reduced number of lactic acid bacteria and coliform in the feces compared with the control group(p<0.05).16S rDNA sequencing analysis of fecal samples showed that L.lucidus supplementation decreased the community of harmful microflora(Enterobacteriaceae including Escherichia coli and Bacteroides sp.)in feces compared with the control group(p<0.05).There were no significant differences in mRNA expression of cytokine IL-6 and IL-10 between the control and L.lucidus fed groups.The fecal fatty acid composition in the L.lucidus group had percentages of 4:0,6:0,8:0 and 10:0 in the intestine but those short chain fatty acids were not detected in the control group.Our results showed that L.lucidus supplementation influenced gut environment by decreasing harmful microflora and increased the percentages of several short fatty acids.
基金This study was supported by the National Nature Science Foundation(81973440)National Key Research and Development Project(2018YFC1707100+1 种基金2018YFC1707103)Heilongjiang Touyan Innovation Team Program.
文摘Objective:The fruit stalk of Schisandra chinensis(Turcz.)Baill.(S.chinensis)has been found to contain bioactive components similar to the fruit of S.chinensis.Here,we report a recent discovery about new nortriterpenoids with a novel skeleton and anti-gastric cancer activity,which were isolated from the fruit stalk of S.chinensis.Methods:The chemical components of ethyl acetate extract from 70%ethanol extract from S.chinensis fruit stalk were separated,purified,and identified by liquid chromatography methods(silica gel,ODS,HPLC)and extensive spectroscopic analyses(NMR,IR,UV,MS,CD).Results:Two new nortriterpenoids,schilancitrilactone M and 25-hydroxyl schindilactone D(1 and 2),along with ten known nortriterpenoids(3-12)were isolated from the fruit stalk of S.chinensis.The isolated compounds were tested for their cytotoxic activities against MGC-803 cells,and the results showed that compounds 6-8 possessed significant activities with IC50 of 9.01,11.77,and 2.74μmol/L,respectively.Conclusion:Twelve nortriterpenoids including two new compounds were isolated from the fruit stalk of S.chinensis for the first time.Among them,compounds 6-8 showed significant anti-gastric cancer activities.We postulated that the fruit stalk of S.chinensis could be used as an anti-gastric cancer drug.
基金Supported by Project of Huai'an Municipal Science and Technology Bureau in Jiangsu Province(SN1082)
文摘[Objectives]The research aimed to optimize the extraction method of triterpenoids from A. selengnesis. [Methods]The ultrasonic extraction of triterpenoids in A. selengnesis was optimized by single factor analysis( including infusion time,ethanol concentration,ultrasonic power,extraction time,and liquid-solid ratio) and response surface method. [Results]The optimum extracting process of triterpenoids was as follows: infusion time of 65 min,ethanol concentration of 80%,ultrasonic power of 275 W,extraction time of 30 min,and liquid-solid ratio of 34 m L/g. Under the optimized condition,the extraction content of triterpenoids reached( 18. 26 ± 0. 10) mg/g. [Conclusions] The optimized extraction had a good modal on practice in developing and utilization.
文摘Schisandra chinensis (Turcz.) Baill. is a deciduous woody vine, and belongs to the Schisandraceae family. This species is a horticultural plant with edible fruit. It is used medicinally and for making rope. It is distributed in Hebei, Heilongjiang, Jilin, Liaoning, Nei Mongol, Shanxi
文摘The qualitative characterization and quantitative analysis of five bioactive flavonoids in Salix bordensis Turcz. were achieved via reversed-phase high-performance liquid chromatography coupled with diode array detection and tandem mass spectrometry, by using an Agilent ZORBAX SB-C18 HPLC column with a gradient elution of 0.3% (v/v) formic acid in water and methanol as the mobile phase. The compounds in the mixture were clearly identified by comparing their HPLC-DAD ultraviolet spectra, retention times, and MS data with those of corresponding reference compounds. All calibration curves showed good linearity (r2 > 0.9998) within the test ranges. The LOD, LOQ, specificity, precision, and accuracy for the method were validated. The results demonstrated that this analytical approach is ideal for the determination of bioactive compounds, such as flavonoids, and that it constructed a basis for the comprehensive evaluation of the quality of Salix bordensis Turcz.
基金Supported by Project of National Natural Science Foundation of China(21503072)Project of China Scholarship Council(20163035)+2 种基金Project of Introduction of Return Talents(XDB-2017-19)Student’s Platform for Innovation and Entrepreneurship Training Program at State Level(201710223002)Guiding Science and Technology Program of Daqing City(ZD-2017-59)
文摘The main components of Artemisia leucophylla(Turcz.ex Bess.) C.B.Clarke extract are volatile oils,flavonoids,lignans,terpenoids,etc.Studies have shown that A.leucophylla has bactericidal,anti-inflammatory,anti-oxidant,anti-ultraviolet functions,and it has large development space and application value in many fields.This paper first introduced the growth resource distribution,active component extraction method,composition and efficacy of A.leucophylla,and analyzed the application prospects in daily necessities,in order to provide reference for the in-depth research,development and utilization of A.leucophylla.
基金Supported by The Cooperative Project between the Yanbian University,ChinaNational Institute of Horticultural and Herbal Science of the Rural Development Administration(RDA)of the Republic of Korea
文摘[ Objectives] This study was conducted to provide high-quality polyploid for returning the grain plots to forestry and promote the rapid development of Lonicera edulis Turcz. [ Methods] With superior individuals of wild L. edulis in Changbai Mountains as experimental materials and colchicine as inducer, polyploid induction was performed twice on diploid plants by addition method, and 7 polyploidy plants were identified from 11 regenerated plantlets. [Results] Among the 7 polyploidy plants, four materials were tetraploid, the concentration and treatment time of which were 300 mg/L and 7 and 14 d, respectively; and three materials were octaploid, the concentrations and treatment time of which were 300 and 500 mg/L and 14 d, respectively. [ Conclusions] The results of this study showed that colchicine had a higher polyploid induction rate for L. edulis with lower toxic and side effects, and the polyploidy plants could restore to normal growth state after several times of cubculture. Therefore, colchicine is an polyploid inducer suitable for L. edulis.
基金This research was funded by the National Natural Science Foundation of China(82260773)National Key R&D Program:Intergovernmental Cooperation in International Science and Technology Innovation(2022YFE0119300)+1 种基金Central Government Guided Local Scientific and Technological Development Project(2021ZY0015)Science and Technology Young Talents Development Project of Inner Mongolia Autonomous Region(NJYT22048).
文摘Corydalis bungeana Turcz.(CB)is a medicinal herb with significant medicinal value in traditional Chinese medicine.This paper reviews the progress of research on CB’s botany,quality control,phytochemistry,pharmacology and toxicity.The plant’s information was gathered from scientific databases such as PubMed,GeenMedical,Springer Link(https://link.springer.com),Chinese National Knowledge Infrastructure,Pharmacopoeia and Flora.Currently,137 phytochemicals have been identified and isolated from CB,including alkaloids,flavonoids,amino acids,terpenoids,coumarins and organic acids.In addition,many phytochemicals reported various antiinflammatory,antibacterial,antiviral,antitumor,analgesic,hepatoprotective,immunomodulatory,neuromodulatory,and lipid reduction activities.However,the study of its toxicity is still at the preliminary exploration stage and needs further intensive exploration.Herein,we provide an in-depth investigation of the progress of CB to elucidate the underlying mechanisms of activity of CB extracts and its major components,deliver valuable resources and information for further research and rational drug use,and explore the potential research directions and prospects of CB.
文摘Objective: To study the effects of n-butanol extract of Rumex gmelini Turcz on the endotoxin-induced acute lung injury (ALI) in mice. Methods: Kunming mice were selected as experimental animals and randomly divided into normal control group (NC group), acute lung injury group (ALI group) and n-butanol extract of Rumex gmelini Turcz group (Rum group). ALI group and Rum group were established into ALI models by intraperitoneal injection of endotoxin, and Rum group were given intragastric administration of n-butanol extract of Rumex gmelini Turcz for intervention before model establishment. 12 h after endotoxin injection, the superior lobe of right lung was taken to determine the water content, and the inferior lobe of right lung was taken to determine the contents of AQPs molecules, inflammatory response molecules and oxidative stress molecules. Results: 12 h after endotoxin injection, the water content of lung tissue in Rum group was (6.82±0.97)%. After variance analysis, the water content of lung tissue in ALI group was significantly higher than that in NC group, AQP1 and AQP5 protein levels in lung tissue were significantly lower than those of NC group, AQP3 and AQP4 protein levels were not different from those of NC group, and MPO, NF-kB, TNF-α, HMGB1, IL-8, ROS, ATP, MDA, Bax and Caspase-3 protein levels were significantly higher than those of NC group;the water content of lung tissue in Rum group was significantly lower than that in ALI group, AQP1 and AQP5 protein levels in lung tissue were significantly higher than those of ALI group, AQP3 and AQP4 protein levels were not different from those of ALI group, and MPO, NF-kB, TNF-α, HMGB1, IL-8, ROS, ATP, MDA, Bax and Caspase-3 protein levels were significantly lower than those of ALI group. Conclusion:The n-butanol extract of Rumex gmelini Turcz reduce the pulmonary edema, inflammatory response, oxidative stress and apoptosis during the endotoxin-induced ALI in mice.
