Accurate determination of lung cancer margins at the molecular level is of great significance to determine the optimal extent of resection during surgical operation and reduce the risk of postoperative recurrence.In t...Accurate determination of lung cancer margins at the molecular level is of great significance to determine the optimal extent of resection during surgical operation and reduce the risk of postoperative recurrence.In this study,internal extractive electrospray ionization mass spectrometry(i EESI-MS)was used to trace potential molecular tumor margins in lung cancer tissue.Molecular differential model for the determination of lung cancer tumor margin was established via partial least-squares discriminant analysis(PLS-DA)of iEESI-MS data collected from lung tissue pieces within cancer tumor area and iEESI-MS data collected from lung tissue pieces outside cancer tumor area.Proof-of-concept data demonstrate that the developed molecular differential model yields ca.1-2 mm wider potential molecular tumor margin of a lung cancer compared to the conventional histological analysis,showing promising potential of iEESI-MS to increase the accuracy of tumor margins determination and lower risk of lung cancer postoperative recurrence.Furthermore,our results revealed that creatine and taurine showed positive correlations with lung cancer.展开更多
目的探讨非小细胞肺癌患者水平裂分化情况及肿瘤边缘-水平裂距离与预后的关系。方法选取2018年7月至2021年7月在烟台市莱阳中心医院胸心外科接受胸腔镜肺叶切除术的91例非小细胞肺癌患者作为研究对象。采用胸部增强薄层电子计算机断层扫...目的探讨非小细胞肺癌患者水平裂分化情况及肿瘤边缘-水平裂距离与预后的关系。方法选取2018年7月至2021年7月在烟台市莱阳中心医院胸心外科接受胸腔镜肺叶切除术的91例非小细胞肺癌患者作为研究对象。采用胸部增强薄层电子计算机断层扫描(CT)联合术中直视探查评估患者水平裂分化情况,良好33例,不全58例。采用胸部增强薄层CT测量58例水平裂分化不全患者肿瘤边缘-水平裂距离,以4 cm为距离临界值,≤4 cm 25例,>4 cm 33例。收集患者一般资料,包括性别、年龄、吸烟、组织学类型、分化程度、TNM分期;术后采用电话回访或门诊复查等随访36个月,每2个月随访1次,记录患者复发情况(淋巴结转移、原位复发及远处转移)和无病生存期。采用χ^(2)检验进行统计学分析;生存分析采用Kaplan-Meier曲线;单因素分析采用Log-rank检验;多因素分析采用COX比例风险回归模型。结果不同水平裂分化情况非小细胞肺癌患者一般资料比较,差异均无统计学意义(均P>0.05);水平裂分化良好患者无病生存期为30.5个月,不全患者为24.6个月,二者比较,差异无统计学意义(P>0.05)。TNM分期(HR=2.592,95%CI:1.739~3.865)和分化程度(HR=3.199,95%CI:1.711~5.982)均是非小细胞肺癌患者预后的独立危险因素(均P<0.05)。不同肿瘤边缘-水平裂距离的水平裂分化不全非小细胞肺癌患者一般资料比较,差异均无统计学意义(均P>0.05);肿瘤边缘-水平裂距离>4 cm患者无病生存期为29.4个月,≤4 cm患者为18.5个月,二者比较,差异有统计学意义(P<0.05);肿瘤边缘-水平裂距离(HR=2.381,95%CI:1.073~5.287)、TNM分期(HR=2.609,95%CI:1.566~4.347)和分化程度(HR=3.384,95%CI:1.539~7.441)均是水平裂分化不全非小细胞肺癌患者预后的独立危险因素(均P<0.05)。结论肿瘤边缘-水平裂距离是水平裂分化不全非小细胞肺癌患者预后的独立危险因素,可评估患者预后,指导综合治疗。展开更多
基金supported by Jiangxi Provincial International Science and Technology Cooperation Project(Nos.20203BDH80W010 and 20232BBH80012)the National Natural Science Foundation of China(Nos.82160410 and 81860379)+1 种基金Foundation of Jiangxi Provincial Department of Science and Technology(No.20212ACB206018)Key Research and Development Program of Jiangxi Province(No.20223BBG71009)。
文摘Accurate determination of lung cancer margins at the molecular level is of great significance to determine the optimal extent of resection during surgical operation and reduce the risk of postoperative recurrence.In this study,internal extractive electrospray ionization mass spectrometry(i EESI-MS)was used to trace potential molecular tumor margins in lung cancer tissue.Molecular differential model for the determination of lung cancer tumor margin was established via partial least-squares discriminant analysis(PLS-DA)of iEESI-MS data collected from lung tissue pieces within cancer tumor area and iEESI-MS data collected from lung tissue pieces outside cancer tumor area.Proof-of-concept data demonstrate that the developed molecular differential model yields ca.1-2 mm wider potential molecular tumor margin of a lung cancer compared to the conventional histological analysis,showing promising potential of iEESI-MS to increase the accuracy of tumor margins determination and lower risk of lung cancer postoperative recurrence.Furthermore,our results revealed that creatine and taurine showed positive correlations with lung cancer.
文摘目的探讨非小细胞肺癌患者水平裂分化情况及肿瘤边缘-水平裂距离与预后的关系。方法选取2018年7月至2021年7月在烟台市莱阳中心医院胸心外科接受胸腔镜肺叶切除术的91例非小细胞肺癌患者作为研究对象。采用胸部增强薄层电子计算机断层扫描(CT)联合术中直视探查评估患者水平裂分化情况,良好33例,不全58例。采用胸部增强薄层CT测量58例水平裂分化不全患者肿瘤边缘-水平裂距离,以4 cm为距离临界值,≤4 cm 25例,>4 cm 33例。收集患者一般资料,包括性别、年龄、吸烟、组织学类型、分化程度、TNM分期;术后采用电话回访或门诊复查等随访36个月,每2个月随访1次,记录患者复发情况(淋巴结转移、原位复发及远处转移)和无病生存期。采用χ^(2)检验进行统计学分析;生存分析采用Kaplan-Meier曲线;单因素分析采用Log-rank检验;多因素分析采用COX比例风险回归模型。结果不同水平裂分化情况非小细胞肺癌患者一般资料比较,差异均无统计学意义(均P>0.05);水平裂分化良好患者无病生存期为30.5个月,不全患者为24.6个月,二者比较,差异无统计学意义(P>0.05)。TNM分期(HR=2.592,95%CI:1.739~3.865)和分化程度(HR=3.199,95%CI:1.711~5.982)均是非小细胞肺癌患者预后的独立危险因素(均P<0.05)。不同肿瘤边缘-水平裂距离的水平裂分化不全非小细胞肺癌患者一般资料比较,差异均无统计学意义(均P>0.05);肿瘤边缘-水平裂距离>4 cm患者无病生存期为29.4个月,≤4 cm患者为18.5个月,二者比较,差异有统计学意义(P<0.05);肿瘤边缘-水平裂距离(HR=2.381,95%CI:1.073~5.287)、TNM分期(HR=2.609,95%CI:1.566~4.347)和分化程度(HR=3.384,95%CI:1.539~7.441)均是水平裂分化不全非小细胞肺癌患者预后的独立危险因素(均P<0.05)。结论肿瘤边缘-水平裂距离是水平裂分化不全非小细胞肺癌患者预后的独立危险因素,可评估患者预后,指导综合治疗。
