Objective:It intended to examine whether there is BDV infection in the human tumor tissues of central nervous system in China and investigate the correlation between BDV infection and tumors of central nervous system....Objective:It intended to examine whether there is BDV infection in the human tumor tissues of central nervous system in China and investigate the correlation between BDV infection and tumors of central nervous system.Methods:Nested reverse transcriptase polymerase chain reaction(nRT-PCR)and fluorescence quantitative polymerase chain reaction(FQ-PCR)was used to detect the BDV p24 fragments in 60 samples of human tumor tissues of central nervous system and 14 normal brain tissues.Results:The study indicated the positive rate of the BDV p24 fragment in human tumor tissues of the central nervous system(6.67%)was higher than that in normal brain tissues(0),but no statistical significance(P>0.05).Conclusion:It suggests that the BDV infection is present in the human tumor tissues of central nervous system in China, while the sample size wasn't large enough and we could not certify the possible correlation between BDV infection and cenfral nervous system tumors.展开更多
Mixed adenoneuroendocrine carcinoma(MANEC) is a rare tumor of the gastrointestinal tract that consists of a dual adenocarcinomatous and neuroendocrine differentiation, each component representing at least 30% of the t...Mixed adenoneuroendocrine carcinoma(MANEC) is a rare tumor of the gastrointestinal tract that consists of a dual adenocarcinomatous and neuroendocrine differentiation, each component representing at least 30% of the tumor. To date, only seven cases have been reported in the cecum, and less than 40 in the stomach. Our first case was diagnosed in a 74-years-old female as a polypoid lesion of the cecum with direct invasion in the transverse colon, without lymph node metastases. The second case was diagnosed in the stomach of a 46-years-old male as a polypoid tumor of the antral region that invaded the pancreas and presented metastases in 22 regional lymph nodes. The metastatic tissue was represented by the glandular component. In both cases, the tumor consisted of a moderately-differentiated tubular adenocarcinoma(with mucinous component in Case 1) intermingled with neuroendocrine carcinoma. Ki67 index was lower than 20% in Case 1, respectively higher than 20% in Case 2. The neuroendocrine component was marked by synaptophysin and neuron specific enolase, being negative for Keratins 7/20. The neuroendocrine component represented 60% in Case 1, and 40% in Case 2, respectively. The glandular components were marked by carcinoembryonic antigen, maspin and keratin 20/7(Case 1/2). Both cases were proved to be microsatellite stable. Independently by the localization and tumor stage, MANECs appear to be highly malignant tumors, with high risk for distant metastases. The aggressiveness seems to depend on the endocrine component, independent of its proportion. The neuroendocrine component could be a dedifferentiated adenocarcinoma with a neuroendocrine phenotype.展开更多
弥漫大B细胞淋巴瘤(diffuse large B cell lymphoma,DLBCL)是临床上较常见的恶性肿瘤,近年来其发病率有明显上升的趋势。乙型肝炎病毒(hepatitis B virus,HBV)感染在世界范围内普遍存在。有研究发现DLBCL患者HBV感染率较一般人群高,二...弥漫大B细胞淋巴瘤(diffuse large B cell lymphoma,DLBCL)是临床上较常见的恶性肿瘤,近年来其发病率有明显上升的趋势。乙型肝炎病毒(hepatitis B virus,HBV)感染在世界范围内普遍存在。有研究发现DLBCL患者HBV感染率较一般人群高,二者存在一定的相关性。展开更多
目的 :建立测定肺组织趋化因子 eotaxin m RNA、α-肿瘤坏死因子 (TNF- α) m RNA表达的方法 ,观察抗炎药物对其表达的影响。方法 :采用半定量 RT- PCR法测定肺组织 eotaxin m RNA、TNF- α m RNA表达 ,并通过观察支气管肺灌洗液中白细...目的 :建立测定肺组织趋化因子 eotaxin m RNA、α-肿瘤坏死因子 (TNF- α) m RNA表达的方法 ,观察抗炎药物对其表达的影响。方法 :采用半定量 RT- PCR法测定肺组织 eotaxin m RNA、TNF- α m RNA表达 ,并通过观察支气管肺灌洗液中白细胞总数和嗜酸性粒细胞的数目来验证 eotaxin和 TNF- α m RNA表达增多的功能。结果 :致敏小鼠抗原攻击后肺组织 eotaxin m RNA和 TNF- α m RNA表达与正常小鼠比较明显增多 ,支气管肺灌洗液中白细胞总数和嗜酸性粒细胞数目相应增加。抗炎药地塞米松对 eotaxin m RNA、TNF- α m RNA表达以及炎症细胞浸润均有明显抑制作用 ;受试的复方中药对 eotaxin m RNA表达以及炎症细胞浸润有明显抑制作用 ,对 TNF- α表达无明显影响。结论 :肺组织 eotaxin m RNA表达与嗜酸性粒细胞增多一致 ,表明半定量 RT- PCR测定肺组织 eo-taxin m RNA的方法可用于探索平喘药物的作用机制。展开更多
Endoscopic submucosal dissection(ESD) is very useful in en bloc resection of large superficial colorectal tumors but is a technically difficult procedure because the colonic wall is thin and endoscopic maneuverability...Endoscopic submucosal dissection(ESD) is very useful in en bloc resection of large superficial colorectal tumors but is a technically difficult procedure because the colonic wall is thin and endoscopic maneuverability is poor because of colonic flexure and extensibility. A high risk of perforation has been reported in colorectal ESD. To prevent complications such as perforation and unexpected bleeding, it is crucial to ensure good visualization of the submucosal layer by creating a mucosal flap, which is an exfoliated mucosa for inserting the tip of the endoscope under it. The creation of a mucosal flap is often technically difficult; however, various types of equipment, appropriate strategy, and novel procedures including our clip-flap method, appear to facilitate mucosal flap creation, improving the safety and success rate of ESD. Favorable treatment outcomes with colorectal ESD have already been reported in many advanced institutions, and appropriate understanding of techniques and development of training systems are required for world-wide standardization of colorectal ESD. Here, we describe recent technical advances for safe and successful colorectal ESD.展开更多
AIM:To analyze the role of CYLD for receptor-mediated cell death of murine hepatocytes in acute liver injury models.METHODS:Hepatocyte cell death in CYLD knockout mice(CYLD-/-)was analyzed by application of liver inju...AIM:To analyze the role of CYLD for receptor-mediated cell death of murine hepatocytes in acute liver injury models.METHODS:Hepatocyte cell death in CYLD knockout mice(CYLD-/-)was analyzed by application of liver injury models for CD95-(Jo2)and tumor necrosis factor(TNF)-α-[D-Gal N/lipopolysaccharide(LPS)]induced apoptosis.Liver injury was assessed by measurement of serum transaminases and histological analysis.Apoptosis induction was quantified by cleaved PARP staining and Western blotting of activated caspases.Nuclear factor(NF)-κB,ERK,Akt and jun amino-terminal kinases signaling were assessed.Primary Hepatocytes were isolated by two step-collagenase perfusion and treated with recombinant TNF-αand with the CD95-ligand Jo2.Cell viability was analyzed by MTT-assay.RESULTS:Livers of CYLD-/-mice showed increased anti-apoptotic NF-κB signaling.In both applied liver injury models CYLD-/-mice showed a significantly reduced apoptosis sensitivity.After D-Gal N/LPS treatment CYLD-/-mice exhibited significantly lower levels of alanine aminotransferase(ALT)(295 U/L vs 859 U/L,P<0.05)and aspartate aminotransferase(AST)(560 U/L vs 1025 U/L,P<0.01).After Jo injection CYLD-/-mice showed 2-fold lower ALT(50 U/L vs 110 U/L,P<0.01)and lower AST(250 U/L vs 435 U/L,P<0.01)serumlevels compared to WT mice.In addition,isolated CYLD-/-primary murine hepatocytes(PMH)were less sensitive towards death receptor-mediated apoptosis and showed increased levels of Bcl-2,XIAP,c IAP1/2,survivin and c-FLIP expression upon TNF-and CD95-receptor triggering,respectively.Inhibition of NF-κB activation by the inhibitor of NF-κB phosphorylation inhibitor BAY 11-7085 inhibited the expression of antiapoptotic proteins and re-sensitized CYLD-/-PMH towards TNF-and CD95-receptor mediated cell death.CONCLUSION:CYLD is a central regulator of apoptotic cell death in murine hepatocytes by controlling NF-κB dependent anti-apoptotic signaling.展开更多
文摘Objective:It intended to examine whether there is BDV infection in the human tumor tissues of central nervous system in China and investigate the correlation between BDV infection and tumors of central nervous system.Methods:Nested reverse transcriptase polymerase chain reaction(nRT-PCR)and fluorescence quantitative polymerase chain reaction(FQ-PCR)was used to detect the BDV p24 fragments in 60 samples of human tumor tissues of central nervous system and 14 normal brain tissues.Results:The study indicated the positive rate of the BDV p24 fragment in human tumor tissues of the central nervous system(6.67%)was higher than that in normal brain tissues(0),but no statistical significance(P>0.05).Conclusion:It suggests that the BDV infection is present in the human tumor tissues of central nervous system in China, while the sample size wasn't large enough and we could not certify the possible correlation between BDV infection and cenfral nervous system tumors.
基金Supported by Romanian government,the research project frame POSDRU/159/1.5/S/136893University of Medicine and Pharmacy of Tirgu-Mures,Romania,the team research project POS-UMFTGM-CC-13-01-V01,No.15/16189/2013
文摘Mixed adenoneuroendocrine carcinoma(MANEC) is a rare tumor of the gastrointestinal tract that consists of a dual adenocarcinomatous and neuroendocrine differentiation, each component representing at least 30% of the tumor. To date, only seven cases have been reported in the cecum, and less than 40 in the stomach. Our first case was diagnosed in a 74-years-old female as a polypoid lesion of the cecum with direct invasion in the transverse colon, without lymph node metastases. The second case was diagnosed in the stomach of a 46-years-old male as a polypoid tumor of the antral region that invaded the pancreas and presented metastases in 22 regional lymph nodes. The metastatic tissue was represented by the glandular component. In both cases, the tumor consisted of a moderately-differentiated tubular adenocarcinoma(with mucinous component in Case 1) intermingled with neuroendocrine carcinoma. Ki67 index was lower than 20% in Case 1, respectively higher than 20% in Case 2. The neuroendocrine component was marked by synaptophysin and neuron specific enolase, being negative for Keratins 7/20. The neuroendocrine component represented 60% in Case 1, and 40% in Case 2, respectively. The glandular components were marked by carcinoembryonic antigen, maspin and keratin 20/7(Case 1/2). Both cases were proved to be microsatellite stable. Independently by the localization and tumor stage, MANECs appear to be highly malignant tumors, with high risk for distant metastases. The aggressiveness seems to depend on the endocrine component, independent of its proportion. The neuroendocrine component could be a dedifferentiated adenocarcinoma with a neuroendocrine phenotype.
文摘弥漫大B细胞淋巴瘤(diffuse large B cell lymphoma,DLBCL)是临床上较常见的恶性肿瘤,近年来其发病率有明显上升的趋势。乙型肝炎病毒(hepatitis B virus,HBV)感染在世界范围内普遍存在。有研究发现DLBCL患者HBV感染率较一般人群高,二者存在一定的相关性。
文摘目的 :建立测定肺组织趋化因子 eotaxin m RNA、α-肿瘤坏死因子 (TNF- α) m RNA表达的方法 ,观察抗炎药物对其表达的影响。方法 :采用半定量 RT- PCR法测定肺组织 eotaxin m RNA、TNF- α m RNA表达 ,并通过观察支气管肺灌洗液中白细胞总数和嗜酸性粒细胞的数目来验证 eotaxin和 TNF- α m RNA表达增多的功能。结果 :致敏小鼠抗原攻击后肺组织 eotaxin m RNA和 TNF- α m RNA表达与正常小鼠比较明显增多 ,支气管肺灌洗液中白细胞总数和嗜酸性粒细胞数目相应增加。抗炎药地塞米松对 eotaxin m RNA、TNF- α m RNA表达以及炎症细胞浸润均有明显抑制作用 ;受试的复方中药对 eotaxin m RNA表达以及炎症细胞浸润有明显抑制作用 ,对 TNF- α表达无明显影响。结论 :肺组织 eotaxin m RNA表达与嗜酸性粒细胞增多一致 ,表明半定量 RT- PCR测定肺组织 eo-taxin m RNA的方法可用于探索平喘药物的作用机制。
文摘Endoscopic submucosal dissection(ESD) is very useful in en bloc resection of large superficial colorectal tumors but is a technically difficult procedure because the colonic wall is thin and endoscopic maneuverability is poor because of colonic flexure and extensibility. A high risk of perforation has been reported in colorectal ESD. To prevent complications such as perforation and unexpected bleeding, it is crucial to ensure good visualization of the submucosal layer by creating a mucosal flap, which is an exfoliated mucosa for inserting the tip of the endoscope under it. The creation of a mucosal flap is often technically difficult; however, various types of equipment, appropriate strategy, and novel procedures including our clip-flap method, appear to facilitate mucosal flap creation, improving the safety and success rate of ESD. Favorable treatment outcomes with colorectal ESD have already been reported in many advanced institutions, and appropriate understanding of techniques and development of training systems are required for world-wide standardization of colorectal ESD. Here, we describe recent technical advances for safe and successful colorectal ESD.
基金Supported by Research grants from the Dietmar Hopp Stiftung,http://www.dietmar-hopp-stiftung.de and from German Research Foundation(Deutsche Forschungsgemeinschaft,http://www.dfg.de/,DFG SCHU 1443/3-2)to HSB(SFB/TRR 77,associated project)
文摘AIM:To analyze the role of CYLD for receptor-mediated cell death of murine hepatocytes in acute liver injury models.METHODS:Hepatocyte cell death in CYLD knockout mice(CYLD-/-)was analyzed by application of liver injury models for CD95-(Jo2)and tumor necrosis factor(TNF)-α-[D-Gal N/lipopolysaccharide(LPS)]induced apoptosis.Liver injury was assessed by measurement of serum transaminases and histological analysis.Apoptosis induction was quantified by cleaved PARP staining and Western blotting of activated caspases.Nuclear factor(NF)-κB,ERK,Akt and jun amino-terminal kinases signaling were assessed.Primary Hepatocytes were isolated by two step-collagenase perfusion and treated with recombinant TNF-αand with the CD95-ligand Jo2.Cell viability was analyzed by MTT-assay.RESULTS:Livers of CYLD-/-mice showed increased anti-apoptotic NF-κB signaling.In both applied liver injury models CYLD-/-mice showed a significantly reduced apoptosis sensitivity.After D-Gal N/LPS treatment CYLD-/-mice exhibited significantly lower levels of alanine aminotransferase(ALT)(295 U/L vs 859 U/L,P<0.05)and aspartate aminotransferase(AST)(560 U/L vs 1025 U/L,P<0.01).After Jo injection CYLD-/-mice showed 2-fold lower ALT(50 U/L vs 110 U/L,P<0.01)and lower AST(250 U/L vs 435 U/L,P<0.01)serumlevels compared to WT mice.In addition,isolated CYLD-/-primary murine hepatocytes(PMH)were less sensitive towards death receptor-mediated apoptosis and showed increased levels of Bcl-2,XIAP,c IAP1/2,survivin and c-FLIP expression upon TNF-and CD95-receptor triggering,respectively.Inhibition of NF-κB activation by the inhibitor of NF-κB phosphorylation inhibitor BAY 11-7085 inhibited the expression of antiapoptotic proteins and re-sensitized CYLD-/-PMH towards TNF-and CD95-receptor mediated cell death.CONCLUSION:CYLD is a central regulator of apoptotic cell death in murine hepatocytes by controlling NF-κB dependent anti-apoptotic signaling.
